FastQCFastQC Report
Fri 27 May 2016
SRR939976_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939976_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1746718
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA42240.24182495399944354No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC34590.19802853122255568No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA25290.14478582118006456No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24980.1430110641786482No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA22150.1268092502624923No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA22110.1265802493590837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19080.109233430925885No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18260.10453891240600943No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA71000.034.739721
TAGGACC31600.032.8036084
ATCAACG80950.030.3943353
GTACATG58050.030.173761
TCAACGC81850.030.0601254
TACATGG56400.029.892812
ACCGTGC2001.8662831E-929.7479618
CAACGCA82900.029.6075275
AACGCAG83200.029.3904136
TATCAAC85350.028.8274332
GCACCGT1454.0706564E-628.722176
ACATGGG54450.028.4468963
GTCGTAT1107.6939084E-427.080811
GGTATCA50050.026.426111
ACGCAGA92600.026.4069397
CGCAGAG93100.026.2012128
CATGGGG26250.026.0992454
TAAGACG3059.094947E-1225.3923534
AGAGTAC72800.025.1713510-11
GAGTACT45750.023.603312-13
Produced by FastQC (version 0.11.5)