FastQCFastQC Report
Fri 27 May 2016
SRR939975_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939975_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2751994
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT200220.7275451908688754No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT111930.40672327047224666No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT87620.3183873220653824No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT64490.23433917370459384No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC63880.23212259910450386No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT59200.21511674807430542No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA52700.19149751053236308No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC51200.18604691725345332No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC46140.16766024925926437No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC43010.156286677950606No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA41930.15236225078979096No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG40040.14549450325836466No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA38800.14098867948113258No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC38050.13826338284167772No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA36220.1316136590414078No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA35860.13030551665446946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC4600.02564.7722108-109
CCGCGCG1400.02282.3418108-109
CGCGACG150.00198418762030.2605110-111
CTCCGCC2550.01910.8334110-111
CGACGCG404.7530193E-91843.43108-109
CCGCTCG1800.01775.1548108-109
CGTCGCT601.0189979E-81522.6953110-111
CCGCGCC3150.01482.5469108-109
CCGCACG850.01445.8275108-109
CCGCGCA357.851535E-61404.5181108-109
CCGCGAC551.6978447E-81340.6763108-109
CCGTTCG602.4043402E-81228.9532108-109
CGCGATC250.0055104291218.1562110-111
TTGTTCG3100.01110.0225108-109
CGCCGCC2750.01107.4149110-111
CGGCGCC1450.01017.0649108-109
CGGATCG300.0079341511015.13025110-111
CAGCTCG1750.0983.1627108-109
CGCCGCT652.6405967E-5937.04333110-111
CGACGCC807.593735E-8921.715108-109
Produced by FastQC (version 0.11.5)