FastQCFastQC Report
Fri 27 May 2016
SRR939975_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939975_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2751994
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT205690.7474216876926331No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT108870.39560406018327077No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT87680.3186053457965388No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT65180.2368464466128923No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC63520.2308144567175655No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT58880.2139539548414713No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA53710.19516757667349566No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC52210.1897169833945859No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC51360.18662831386987036No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC43260.15719511016375762No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG41550.15098143382580048No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA40880.14854683549455414No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA39770.14451339646816092No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA37640.13677355401210903No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA36400.13226773023487698No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC36250.13172267090698597No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29120.10581418418790157No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCGC1004.442175E-429.7477726
GTACATG89650.029.3071021
TACATGG89850.028.6409232
GTACGGC850.00717131528.032543
ACATGGG88250.026.3251763
TAGGACC43900.024.8317874
CATGGGG46200.023.9823844
GAGTACT56250.023.00494612-13
GTCCTAC130450.022.8354991
AGTACTT61800.022.14235512-13
AGAGTAC112750.021.89858210-11
CGTATAC1653.1916998E-421.6615073
CTGGTCG1653.2184803E-421.6347459
AGTGGAC91050.021.5634618
TCCTACA142150.021.4138812
TACAGTG147800.021.2804535
GGGCCGT1400.00315813521.2484116
CTACAGT145350.021.1883374
CCTACAG137800.020.965923
GTAGGAC147100.020.9362683
Produced by FastQC (version 0.11.5)