FastQCFastQC Report
Fri 27 May 2016
SRR939974_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939974_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295894
Sequences flagged as poor quality0
Sequence length125
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT137841.0636672443888158No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT79790.6157139395660448No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT61090.47141201363691787No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT41190.3178500710706277No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC39880.30774121957505784No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT38690.2985583697432043No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC35370.27293899038038605No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC34480.26607114470782334No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA30400.23458708814146836No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG29000.22378373539811125No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC27410.21151421335387No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA24780.1912193435574206No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA18570.1432987574601009No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC18190.14036641885833254No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC16790.12956306611497545No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT16520.12747956237161373No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG15590.12030304947781223No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA14530.11212336811498472No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC14140.10911386270790666No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG13140.1013971821769373No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG52.1363462E-46183.44110-111
CCGCTCC7500.0896.91113108-109
CGGTGCG753.85861E-5824.4587110-111
CCGCGCC7500.0736.7484108-109
CCTCGCC6300.0724.54553108-109
CGACGCC1002.0160951E-7720.7321108-109
CCGTTCG758.234917E-5640.65076108-109
CTCCGCC3200.0579.6975110-111
GTTGTCG1101.2163527E-4562.1309110-111
CGTCGCT1101.2163527E-4562.1309110-111
CGTCACT2001.1695629E-6463.75803110-111
CCGCGCT1052.25756E-4457.6077108-109
CCGCTCG2157.612471E-9446.96567108-109
CGTCGCG1101.3151111E-7436.80734108-109
CCGCACG1152.965038E-4417.81573108-109
CCGCTAC1203.3683187E-4400.40674108-109
TTTTTCG6400.0384.1111592-93
CGGCGCG2255.145748E-6320.32538108-109
TGTCGCT1956.7623466E-4317.09952110-111
CCCCTCG5950.0316.7571792-93
Produced by FastQC (version 0.11.5)