##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939974_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1295894 Sequences flagged as poor quality 0 Sequence length 125 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.021673840607335 34.0 31.0 34.0 30.0 34.0 2 32.13003224029126 34.0 31.0 34.0 30.0 34.0 3 32.233231267372176 34.0 31.0 34.0 30.0 34.0 4 35.47667478975904 37.0 35.0 37.0 35.0 37.0 5 35.50349488461248 37.0 35.0 37.0 35.0 37.0 6 35.63755754714506 37.0 37.0 37.0 35.0 37.0 7 35.632070215619486 37.0 37.0 37.0 35.0 37.0 8 35.63605202277347 37.0 37.0 37.0 35.0 37.0 9 37.351791890386096 39.0 39.0 39.0 35.0 39.0 10-11 37.337887203737345 39.0 39.0 39.0 35.0 39.0 12-13 37.2909937078187 39.0 39.0 39.0 35.0 39.0 14-15 38.81795270292169 41.0 40.0 41.0 36.0 41.0 16-17 38.8248518011504 41.0 40.0 41.0 36.0 41.0 18-19 38.849846901058264 41.0 40.0 41.0 36.0 41.0 20-21 38.88262041494134 41.0 40.0 41.0 36.0 41.0 22-23 38.85894911157857 41.0 39.5 41.0 35.0 41.0 24-25 38.8609434876618 41.0 39.0 41.0 35.0 41.0 26-27 38.79919383838493 41.0 39.0 41.0 35.0 41.0 28-29 38.74390150737638 41.0 39.0 41.0 35.0 41.0 30-31 38.680041731808316 41.0 39.0 41.0 35.0 41.0 32-33 38.54273806345272 41.0 39.0 41.0 34.0 41.0 34-35 38.415581444161326 40.0 38.5 41.0 34.0 41.0 36-37 38.5864565311669 41.0 39.0 41.0 35.0 41.0 38-39 38.55603969151798 41.0 39.0 41.0 34.0 41.0 40-41 38.517933951388 41.0 39.0 41.0 34.0 41.0 42-43 38.39826791388802 41.0 39.0 41.0 34.0 41.0 44-45 38.25637474978663 41.0 38.0 41.0 33.5 41.0 46-47 38.12201576672166 40.5 38.0 41.0 33.0 41.0 48-49 37.975804734029175 40.0 38.0 41.0 33.0 41.0 50-51 37.81428072049103 40.0 38.0 41.0 33.0 41.0 52-53 37.67194809143341 40.0 37.5 41.0 33.0 41.0 54-55 37.4525011304937 40.0 37.0 41.0 32.5 41.0 56-57 37.18062318368632 40.0 36.5 41.0 31.5 41.0 58-59 36.954778322918386 40.0 36.0 41.0 31.0 41.0 60-61 36.640760741233464 39.0 35.0 41.0 31.0 41.0 62-63 36.374577704657945 39.0 35.0 41.0 31.0 41.0 64-65 36.04804482465387 38.5 35.0 41.0 31.0 41.0 66-67 35.660103372652394 37.5 35.0 40.0 30.0 41.0 68-69 35.23605595828054 37.0 35.0 40.0 30.0 41.0 70-71 34.79870305750316 36.0 35.0 39.0 29.5 41.0 72-73 34.4041985687101 36.0 34.0 39.0 29.0 41.0 74-75 34.02369329590229 35.0 34.0 38.0 29.0 40.0 76-77 33.61132855002029 35.0 34.0 37.0 29.0 39.0 78-79 33.16780230481814 35.0 34.0 37.0 28.5 39.0 80-81 32.73681296464063 35.0 34.0 36.0 27.0 38.5 82-83 32.625270662569626 35.0 34.0 36.0 28.0 37.0 84-85 32.306857273820235 35.0 34.0 35.5 27.5 37.0 86-87 31.738965918508768 35.0 34.0 35.0 23.5 36.5 88-89 16.434576824956363 18.0 16.5 18.5 2.0 19.0 90-91 2.814751438003417 2.0 2.0 2.0 2.0 2.0 92-93 2.3006781418850615 2.0 2.0 2.0 2.0 2.0 94-95 2.1024153981729987 2.0 2.0 2.0 2.0 2.0 96-97 2.0000235358756195 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.11477954204922725 1101 2 0.19397413453398116 1101 3 0.19790431230499905 1101 4 0.2678314471349381 1101 5 0.24951925907767958 1101 6 0.13389998801169867 1101 7 0.1730172701254986 1101 8 0.29283831475179056 1101 9 0.28758438837860467 1101 10-11 0.33893180532549394 1101 12-13 0.36902845034421716 1101 14-15 0.3296659169101801 1101 16-17 0.32875315751995515 1101 18-19 0.27191251071837996 1101 20-21 0.311619843883328 1101 22-23 0.2989225311809278 1101 24-25 0.2703329051349286 1101 26-27 0.31912253969075977 1101 28-29 0.3552570651614815 1101 30-31 0.2606352870950417 1101 32-33 0.37430245539359674 1101 34-35 0.3207901311905772 1101 36-37 0.34378125938436455 1101 38-39 0.31690581059284284 1101 40-41 0.23860112662136856 1101 42-43 0.15863131906034056 1101 44-45 0.2612536530683798 1101 46-47 0.2814103959338823 1101 48-49 0.25384973761760676 1101 50-51 0.29220566448615415 1101 52-53 0.23722724482074398 1101 54-55 0.21007978721587506 1101 56-57 0.3541572488835385 1101 58-59 0.24283829353512232 1101 60-61 0.28430571424920714 1101 62-63 0.2709196480618772 1101 64-65 0.22790041021738006 1101 66-67 0.15969908149556744 1101 68-69 0.2781224740761772 1101 70-71 0.24342062674801923 1101 72-73 0.2833965070343183 1101 74-75 0.30693697925639896 1101 76-77 0.22774501424561322 1101 78-79 0.25569834299306393 1101 80-81 0.28825202170268227 1101 82-83 0.39198597802665347 1101 84-85 0.3884163720188596 1101 86-87 0.4880324003653449 1101 88-89 1.1414884307664543 1101 90-91 0.05304645127993135 1101 92-93 -0.16533414927201884 1101 94-95 -0.008362320486837937 1101 96-97 -2.419098984773882E-4 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.04267424480158155 1102 2 0.034434188457076687 1102 3 0.14822662745221038 1102 4 0.24660405221202808 1102 5 0.19276065097665196 1102 6 0.25140942389967336 1102 7 0.2643240841810979 1102 8 0.25134265208715334 1102 9 0.25076103592161303 1102 10-11 0.3116827142391614 1102 12-13 0.29470917875747915 1102 14-15 0.30668971778857923 1102 16-17 0.37104732954655617 1102 18-19 0.32064202703956823 1102 20-21 0.26592129376172835 1102 22-23 0.2975123001856801 1102 24-25 0.29925768823544985 1102 26-27 0.3373946975895237 1102 28-29 0.3297592317028588 1102 30-31 0.33459455886497125 1102 32-33 0.3177126432084947 1102 34-35 0.3255957443744535 1102 36-37 0.36095820657283184 1102 38-39 0.27503640890893877 1102 40-41 0.330529312464364 1102 42-43 0.3035303457359788 1102 44-45 0.2226877117661772 1102 46-47 0.38883051392092227 1102 48-49 0.31537519472414033 1102 50-51 0.298389286964273 1102 52-53 0.34339464195501535 1102 54-55 0.2450153078302435 1102 56-57 0.21063132464411893 1102 58-59 0.32491219997638865 1102 60-61 0.1322994308539478 1102 62-63 0.22907396285840775 1102 64-65 0.1936081786895798 1102 66-67 0.24444460856735617 1102 68-69 0.10653275675699803 1102 70-71 0.18656502642010508 1102 72-73 0.2640026413919401 1102 74-75 0.1793809151322705 1102 76-77 0.12155766255539646 1102 78-79 0.08473097883288716 1102 80-81 0.0333629919324423 1102 82-83 0.019554472857777228 1102 84-85 0.05531922211827833 1102 86-87 0.11608929113149458 1102 88-89 1.2167344442714345 1102 90-91 0.14213569263388415 1102 92-93 0.005007263366608061 1102 94-95 0.13438832018525781 1102 96-97 -2.419098984773882E-4 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.0017821287840433797 1103 2 0.2272459274001406 1103 3 0.16051065014235633 1103 4 0.06414080844329817 1103 5 0.044918294368549994 1103 6 -0.05938117332018322 1103 7 -0.0179422243607803 1103 8 0.08973048920719151 1103 9 0.11796818872931425 1103 10-11 0.0371794996348882 1103 12-13 0.07243263509597853 1103 14-15 0.02028144300130208 1103 16-17 0.14049061170011612 1103 18-19 0.10522219922008702 1103 20-21 0.17033222942955462 1103 22-23 0.20004032461967114 1103 24-25 0.0972568914746077 1103 26-27 0.19942728961790834 1103 28-29 0.09946935357218933 1103 30-31 0.08029599654224029 1103 32-33 0.18765271075778145 1103 34-35 0.1029089189903516 1103 36-37 0.1844537567386979 1103 38-39 0.09087096577962939 1103 40-41 0.12591273482777865 1103 42-43 0.13582252394984096 1103 44-45 0.09281538141610213 1103 46-47 0.11041960080293478 1103 48-49 0.018528225421981404 1103 50-51 0.10272812579810164 1103 52-53 0.07123253870683044 1103 54-55 0.032276404854670204 1103 56-57 0.1324056333663961 1103 58-59 0.1730227743457391 1103 60-61 0.2112554862363467 1103 62-63 0.017833997535042556 1103 64-65 -0.054362962532522374 1103 66-67 0.07659804265669834 1103 68-69 0.02891872975732923 1103 70-71 0.19392097055665403 1103 72-73 0.24902203425016012 1103 74-75 0.18211351668169584 1103 76-77 0.2143273711113025 1103 78-79 0.2254757748320415 1103 80-81 0.3104340690127927 1103 82-83 0.3552764183573274 1103 84-85 0.22268127976568053 1103 86-87 0.3004918207218843 1103 88-89 0.8457173089022696 1103 90-91 -0.6084969555185329 1103 92-93 -0.341821728705872 1103 94-95 -0.11985856543195617 1103 96-97 -2.419098984773882E-4 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 0.038188452676038764 1104 2 0.21082457520311237 1104 3 0.1774119132679175 1104 4 0.13197621819124805 1104 5 0.22840623900601997 1104 6 0.2452254916827954 1104 7 0.26692810370632714 1104 8 0.1391229219716692 1104 9 0.3074574195280775 1104 10-11 0.22375867054076792 1104 12-13 0.3111805234878986 1104 14-15 0.38193452019017116 1104 16-17 0.36407451452774353 1104 18-19 0.2925410328445679 1104 20-21 0.2571883089412026 1104 22-23 0.2059426291519486 1104 24-25 0.2543377372696298 1104 26-27 0.301348373064819 1104 28-29 0.33729461320400134 1104 30-31 0.3157277004180017 1104 32-33 0.3882170860097389 1104 34-35 0.3353159842390454 1104 36-37 0.37133467164089495 1104 38-39 0.3765529432046151 1104 40-41 0.41855162105049715 1104 42-43 0.3999972931126763 1104 44-45 0.2644045344692998 1104 46-47 0.36173335696978626 1104 48-49 0.34709264386592764 1104 50-51 0.3698705441652521 1104 52-53 0.2827424824612734 1104 54-55 0.3736010549995967 1104 56-57 0.3151311195161668 1104 58-59 0.2582861494633235 1104 60-61 0.24635917099244864 1104 62-63 0.27191683975952685 1104 64-65 0.10495666774409784 1104 66-67 0.18494153410468783 1104 68-69 0.24061703855121408 1104 70-71 0.12004768143911093 1104 72-73 0.29281390647867056 1104 74-75 0.15274836685350124 1104 76-77 0.2037903267867307 1104 78-79 0.16728177563829405 1104 80-81 0.1333613337124646 1104 82-83 0.05703577182132591 1104 84-85 0.14132617927233326 1104 86-87 0.19020588979066488 1104 88-89 0.919679381541588 1104 90-91 -0.6254402108503436 1104 92-93 -0.341821728705872 1104 94-95 -0.11985856543195617 1104 96-97 -2.419098984773882E-4 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 0.023458170191346994 1105 2 -0.024654435512466932 1105 3 0.02810959608072494 1105 4 0.1589232271323482 1105 5 0.17291297655875582 1105 6 0.06807466202009493 1105 7 0.06650264759851865 1105 8 0.07892623875948601 1105 9 0.10247641601521451 1105 10-11 0.12450668269199383 1105 12-13 0.20662197786315062 1105 14-15 0.23577698615892473 1105 16-17 0.2872652199302479 1105 18-19 0.22701465025775747 1105 20-21 0.2141674948925285 1105 22-23 0.09871945215685685 1105 24-25 0.06532123483554386 1105 26-27 0.07658683341205119 1105 28-29 0.007924023934485547 1105 30-31 -0.01945375882426248 1105 32-33 -0.044764553242139016 1105 34-35 0.034757558543077494 1105 36-37 -0.03167073796249298 1105 38-39 -0.015664697272164574 1105 40-41 0.07332336047202404 1105 42-43 0.015669918959318352 1105 44-45 -0.002870013963892859 1105 46-47 0.11517956308662036 1105 48-49 0.04646261020435105 1105 50-51 -0.07750560607407664 1105 52-53 -0.036250336948100426 1105 54-55 0.012194488258060687 1105 56-57 0.02173465473738645 1105 58-59 -0.09956010415928063 1105 60-61 -0.1559134213206903 1105 62-63 0.0376211070121002 1105 64-65 0.03938065443316674 1105 66-67 0.04621390868303621 1105 68-69 0.1331777277575057 1105 70-71 -0.010390012062494236 1105 72-73 0.04393722005286804 1105 74-75 -0.04331759048677952 1105 76-77 0.02322778508818857 1105 78-79 -0.04674846621033879 1105 80-81 -0.15448536818764325 1105 82-83 -0.07197005345756224 1105 84-85 -0.05179439107638473 1105 86-87 0.11939652766045228 1105 88-89 0.9408481225105412 1105 90-91 -0.8374792564034887 1105 92-93 -0.341821728705872 1105 94-95 -0.11985856543195617 1105 96-97 -2.419098984773882E-4 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.09811867225099036 1106 2 -0.03911924698820002 1106 3 0.022789411204300336 1106 4 -0.013162679323485804 1106 5 -0.03723292895264052 1106 6 0.021371039069194353 1106 7 0.01680530296873428 1106 8 0.01138587702603644 1106 9 0.0663349248042806 1106 10-11 0.016731165433945705 1106 12-13 0.024199293086610396 1106 14-15 0.01577532639536372 1106 16-17 -0.03863159027802254 1106 18-19 -0.012750210982929389 1106 20-21 -0.03133682314722108 1106 22-23 -0.05648297053910056 1106 24-25 -0.10911348853579739 1106 26-27 -0.2723322106423751 1106 28-29 -0.03260703893170813 1106 30-31 0.039675876812928834 1106 32-33 0.058423142824409524 1106 34-35 -0.093652116928439 1106 36-37 0.00944724971110844 1106 38-39 -0.011663521466601878 1106 40-41 0.1574392835003522 1106 42-43 -0.24749020379613285 1106 44-45 0.13906408001058423 1106 46-47 -0.06530267925953837 1106 48-49 -0.04340240966390496 1106 50-51 -0.11115787107852526 1106 52-53 -0.03694679497354514 1106 54-55 -0.06682562533794112 1106 56-57 -0.2621317169719717 1106 58-59 -0.12220106740334558 1106 60-61 0.12394744355024301 1106 62-63 0.22628662637418984 1106 64-65 0.140424908368054 1106 66-67 0.03700277977278432 1106 68-69 0.06900201971047437 1106 70-71 0.052116571806003265 1106 72-73 0.11458684424495402 1106 74-75 0.0882984464057941 1106 76-77 0.024240827644355534 1106 78-79 0.016404978371859613 1106 80-81 0.010785448502645067 1106 82-83 0.10592712111630931 1106 84-85 0.17197526727218104 1106 86-87 0.18154335638039854 1106 88-89 0.9276570538267883 1106 90-91 -0.2539290221504955 1106 92-93 -0.30303984427068187 1106 94-95 -0.11985856543195617 1106 96-97 -2.419098984773882E-4 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.03362878715661566 1107 2 0.019438258843088363 1107 3 0.125569598003068 1107 4 0.09082796818485406 1107 5 0.10655388466445714 1107 6 0.08867126564329908 1107 7 0.026744862731760577 1107 8 -0.007467075234750098 1107 9 0.08571458238705532 1107 10-11 0.07046338812774167 1107 12-13 0.18025724562917844 1107 14-15 0.23944293153410712 1107 16-17 0.1887875883661536 1107 18-19 0.07550818658823744 1107 20-21 0.04867337442335895 1107 22-23 0.12887309908536082 1107 24-25 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46-47 -0.20097659369619691 2215 48-49 -0.2494342977531474 2215 50-51 -0.1952310286199932 2215 52-53 -0.29550206057832185 2215 54-55 -0.2510551764064459 2215 56-57 -0.22474556887370767 2215 58-59 -0.15001044494361793 2215 60-61 -0.25371672749098195 2215 62-63 -0.35749499384476024 2215 64-65 -0.22395503233507696 2215 66-67 -0.2110285843812818 2215 68-69 -0.22907932066412684 2215 70-71 -0.1707686020651522 2215 72-73 -0.268936068924603 2215 74-75 -0.2841297458042078 2215 76-77 -0.28206292209002015 2215 78-79 -0.273726713747628 2215 80-81 -0.2598229121986222 2215 82-83 -0.2347711742954317 2215 84-85 -0.31963974605668 2215 86-87 -0.44351704794915037 2215 88-89 -1.3003502048116786 2215 90-91 -0.8374792564034887 2215 92-93 -0.341821728705872 2215 94-95 -0.11985856543195617 2215 96-97 -2.419098984773882E-4 2215 98-99 0.0 2215 100-101 0.0 2215 102-103 0.0 2215 104-105 0.0 2215 106-107 0.0 2215 108-109 0.0 2215 110-111 0.0 2215 112-113 0.0 2215 114-115 0.0 2215 116-117 0.0 2215 118-119 0.0 2215 120-121 0.0 2215 122-123 0.0 2215 124-125 0.0 2216 1 0.10976038108717745 2216 2 -0.016772713389265448 2216 3 -0.021472919639712984 2216 4 0.04226778541409004 2216 5 0.04530868929005294 2216 6 0.0706616928602557 2216 7 -0.0034444385680671985 2216 8 -0.0619190770408693 2216 9 -0.05283254352248434 2216 10-11 -0.0840367600636327 2216 12-13 -0.11617787711669791 2216 14-15 -0.08366200018192416 2216 16-17 -0.24261519577656543 2216 18-19 -0.12981190466223325 2216 20-21 -0.1726583411014886 2216 22-23 -0.19239451260518337 2216 24-25 -0.20310223549508777 2216 26-27 -0.2314250892914984 2216 28-29 -0.21539687594911783 2216 30-31 -0.20409897767988383 2216 32-33 -0.250831229876745 2216 34-35 -0.29386934578375445 2216 36-37 -0.20477906952056202 2216 38-39 -0.33059716454228294 2216 40-41 -0.3361416968590163 2216 42-43 -0.36515255440183125 2216 44-45 -0.3783465229475951 2216 46-47 -0.4144534185904689 2216 48-49 -0.3140134863893067 2216 50-51 -0.25072243811577977 2216 52-53 -0.29317407919153027 2216 54-55 -0.3025170085149256 2216 56-57 -0.40325732901752787 2216 58-59 -0.28811497916099427 2216 60-61 -0.24434197706016647 2216 62-63 -0.33591606609160607 2216 64-65 -0.4354724839874322 2216 66-67 -0.4540595478203926 2216 68-69 -0.49581567444275976 2216 70-71 -0.5281207994275761 2216 72-73 -0.5303662046369908 2216 74-75 -0.4908925605719574 2216 76-77 -0.5658855145570669 2216 78-79 -0.5312677407037683 2216 80-81 -0.5568380203408267 2216 82-83 -0.5634250890488133 2216 84-85 -0.6124868491019129 2216 86-87 -0.7752511567142868 2216 88-89 -1.4880305823288893 2216 90-91 -0.8374792564034887 2216 92-93 -0.341821728705872 2216 94-95 -0.11985856543195617 2216 96-97 -2.419098984773882E-4 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores warn #Quality Count 2 9359.0 3 1729.0 4 1608.0 5 1803.0 6 2107.0 7 2100.0 8 2075.0 9 2277.0 10 2664.0 11 3183.0 12 3816.0 13 4077.0 14 4434.0 15 4819.0 16 5352.0 17 6542.0 18 8189.0 19 10112.0 20 11270.0 21 15531.0 22 18983.0 23 26237.0 24 42269.0 25 76865.0 26 171277.0 27 434847.0 28 407871.0 29 14048.0 30 450.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.874884828543074 18.864274392812995 14.100458833824373 23.160381944819562 2 15.17162775120651 21.56462837008811 37.986674753797786 25.27706912490759 3 19.88236420314447 24.985293595418703 29.08008158387591 26.05226061756091 4 12.710659898477159 17.304047403061126 37.7742178301905 32.21107486827122 5 14.124619183133344 38.09928955361237 33.46571528718186 14.310375976072425 6 31.209342739452456 35.89830649729067 17.812799503663108 15.079551259593762 7 28.120046855684183 32.40990389646067 21.591657959678802 17.87839128817635 8 25.794085010039403 32.8232864725047 20.67082647191823 20.711802045537674 9 25.669460619464246 17.957795930840025 20.933579443997736 35.439164005698 10-11 25.07726380041439 25.988147096385866 27.286641947395022 21.64794715580472 12-13 26.72986769455152 23.70073271521339 26.958325134176786 22.611074456058308 14-15 23.229849995331406 25.493076974719365 26.03471119914993 25.242361830799304 16-17 22.598737085140648 28.373451162173364 26.42934475198917 22.598467000696818 18-19 22.53955355217002 28.755527927973812 26.678481702322067 22.0264368175341 20-21 24.07673353855082 26.754528456369936 25.684660623323026 23.484077381756222 22-23 23.224977525011862 26.802648383151283 25.951646943980368 24.020727147856487 24-25 24.309434259283552 26.93491905973791 25.576320285455445 23.179326395523088 26-27 23.56431158721315 26.270512866021452 27.13678742242807 23.02838812433733 28-29 22.64816697565935 28.17866657920984 26.159609994000206 23.013556451130604 30-31 23.308387877403554 26.927511046428183 25.859445294136712 23.904655782031554 32-33 23.297733191858782 27.04844217227742 26.04888588791357 23.60493874795023 34-35 24.257133883738856 26.753559128724362 25.641777523298604 23.347529464238175 36-37 24.005116165114003 26.20745933786946 26.641947538086686 23.14547695892985 38-39 23.406351136744206 27.02447113729981 26.352772680481586 23.216405045474396 40-41 23.250397023213317 27.160709132074075 26.38931116279572 23.199582681916887 42-43 23.26737372038145 27.218275568835104 25.24376993797332 24.27058077281012 44-45 23.57901876816076 26.344257987647886 25.662835198780144 24.41388804541121 46-47 24.084956022637655 27.09700793429092 25.77367439003499 23.044361653036436 48-49 23.764480373364446 26.664760643633883 26.07370058615996 23.497058396841712 50-51 22.64257157033798 27.00840269991427 27.03363626472247 23.315389465025277 52-53 23.539695376319358 27.519573360166806 25.287986517415778 23.65274474609806 54-55 22.81187506052858 26.50277746589082 25.999334040963042 24.686013432617564 56-57 23.799979010628956 26.299874835441788 25.878042494216345 24.022103659712908 58-59 24.742417203876244 25.999155795149914 25.57427536511474 23.684151635859106 60-61 23.57735277731049 26.547888947707143 26.864118515866263 23.0106397591161 62-63 22.974534558588342 26.78020994780821 26.529070483029667 23.716185010573785 64-65 22.798380266881704 27.64179899181452 25.853263291515304 23.706557449788466 66-67 23.412948898598188 26.488123256994783 25.43483494792012 24.664092896486903 68-69 22.95073681221311 26.509343217943854 26.07562467855017 24.464295291292874 70-71 23.9901566023147 26.486695671096555 25.59156072950411 23.93158699708464 72-73 23.55157906433705 25.704147098450953 26.9093768471804 23.83489699003159 74-75 22.75571921777553 27.694934925233085 25.81445704664116 23.73488881035023 76-77 23.336823845160175 26.18269704157902 25.914928227154384 24.565550886106426 78-79 23.540026784711802 26.368742372511218 26.267306176418725 23.823924666358256 80-81 23.851294936159903 26.40293110393288 25.974578167658773 23.771195792248438 82-83 23.36570118710425 26.096230026672902 26.76889799978316 23.769170786439688 84-85 23.186868051606112 26.446666507188475 26.41363908902973 23.952826352175684 86-87 23.246623815427142 26.620319729048674 26.177454136325277 23.955602319198906 88-89 32.64261102603369 17.21362940275651 32.10796324655437 18.035796324655436 90-91 31.632046360514497 13.254810787123683 15.263525869403452 39.849616982958366 92-93 23.972228808669904 15.989174248978061 32.440426388317285 27.598170554034752 94-95 16.31428385716073 6.589958085887238 26.799381876916346 50.296376180035686 96-97 28.442329390679937 7.160188032712214 14.748778650725367 49.64870392588248 98-99 57.746073718391976 14.267933107999712 18.76427927354801 9.221713900060298 100-101 32.369865537516404 6.803728610046092 11.969876973021737 48.85652887941577 102-103 24.91378014614871 2.058188442638233 35.634856557595846 37.39317485361721 104-105 45.32214532765968 4.558530241620485 36.400997484843195 13.718326945876644 106-107 5.997081066737429 1.8252809947118027 6.987959926795007 85.18967801175576 108-109 19.568456223852905 9.079865042693955 19.17764861217609 52.17403012127705 110-111 41.36515692968135 2.504176799448929 23.319734156387998 32.81093211448173 112-113 29.2040729237522 2.8350680852702235 37.27123092521529 30.689628065762285 114-115 29.236722555264144 3.9349358373922816 22.25728269014612 44.571058917197455 116-117 20.175534366190384 4.225742205591495 19.754109382021117 55.84461404619701 118-119 21.144172471050553 4.394799561919618 15.166223241631767 59.294804725398066 120-121 26.521999430753073 4.283484968936784 29.07811441863204 40.1164011816781 122-123 35.064953734540246 1.6185323794326751 33.365119373351796 29.951394512675282 124-125 39.563733238119134 1.6963877598447432 17.99117524389662 40.748703758139506 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 72.0 1 159.5 2 321.5 3 467.5 4 538.5 5 540.0 6 468.5 7 366.5 8 327.0 9 275.5 10 243.0 11 231.5 12 200.0 13 171.0 14 167.5 15 179.0 16 186.0 17 216.0 18 283.5 19 371.5 20 525.0 21 775.0 22 1157.0 23 1777.0 24 2664.0 25 3755.5 26 5083.5 27 6594.5 28 8439.5 29 10446.0 30 12553.0 31 15053.0 32 17623.0 33 20166.0 34 22858.5 35 25505.5 36 28147.5 37 30918.0 38 33433.5 39 35912.5 40 38343.5 41 40795.0 42 44976.5 43 52271.5 44 60155.0 45 62912.0 46 60629.5 47 58656.0 48 58602.0 49 58930.0 50 58132.0 51 55020.0 52 49753.0 53 44196.5 54 39704.0 55 35839.0 56 31976.0 57 28122.5 58 24347.0 59 20783.0 60 17573.5 61 14602.0 62 11749.0 63 9148.5 64 7115.5 65 5541.0 66 4271.0 67 3274.5 68 2426.0 69 1804.0 70 1345.0 71 997.5 72 758.5 73 598.0 74 445.0 75 313.5 76 220.0 77 139.0 78 71.0 79 45.0 80 33.0 81 20.0 82 13.0 83 11.5 84 13.0 85 10.0 86 5.5 87 7.5 88 8.0 89 6.5 90 5.5 91 7.5 92 7.5 93 4.5 94 5.0 95 5.5 96 5.5 97 4.0 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 6.173344424775483E-4 3 0.041670074867234515 4 0.0477662524867003 5 0.050312757061920184 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.945012477872418E-4 12-13 0.002237837353981113 14-15 7.716680530969354E-5 16-17 2.315004159290806E-4 18-19 0.006752095464598184 20-21 0.0025465045752198867 22-23 6.945012477872418E-4 24-25 0.0 26-27 0.0 28-29 0.0012732522876099433 30-31 0.0 32-33 0.0014661693008841772 34-35 7.716680530969354E-5 36-37 1.157502079645403E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 1.9291701327423387E-4 46-47 0.0 48-49 1.5433361061938707E-4 50-51 7.716680530969354E-5 52-53 0.0 54-55 0.0014275858982293304 56-57 0.0 58-59 0.0 60-61 0.0 62-63 3.858340265484677E-5 64-65 1.157502079645403E-4 66-67 0.0 68-69 5.787510398227015E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 4.2441742920331445E-4 80-81 0.0 82-83 7.330846504420886E-4 84-85 7.716680530969354E-5 86-87 0.0011960854823002498 88-89 19.37612181243219 90-91 26.21630318529139 92-93 40.28095662145206 94-95 44.795523399290374 96-97 48.1594945265585 98-99 93.025316885486 100-101 51.41439037452137 102-103 53.02366551585238 104-105 74.81113424400453 106-107 81.67940433399646 108-109 12.596670715351719 110-111 39.17071921005885 112-113 51.89915224547686 114-115 86.81867498421938 116-117 66.42692226370367 118-119 71.60500781699739 120-121 80.34387843450159 122-123 75.84443635050397 124-125 49.8598650815576 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1295894.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.765147644106264 #Duplication Level Percentage of deduplicated Percentage of total 1 67.6033487174725 28.910671891387135 2 15.011442592655111 12.839331176518423 3 6.284065745134424 8.06216998187833 4 3.36836598134332 5.761946740461278 5 2.039466218593433 4.360903697665763 6 1.2937392595738022 3.319617026919022 7 0.9421720941126425 2.8204490097618757 8 0.6436812519792506 2.202169902130866 9 0.47925086838181 1.8445710730422817 >10 2.1622981897648383 15.577826433460052 >50 0.10825025324928625 3.1878326764691947 >100 0.056335246195504826 4.304794760777832 >500 0.0028889834453672486 0.8043734186993738 >1k 0.00415291370271542 3.8511394724212895 >5k 3.611229306709061E-4 1.0878384105439933 >10k+ 1.8056146533545304E-4 1.0643643278633166 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13784 1.0636672443888158 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7979 0.6157139395660448 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6109 0.47141201363691787 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4119 0.3178500710706277 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3988 0.30774121957505784 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3869 0.2985583697432043 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3537 0.27293899038038605 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3448 0.26607114470782334 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3040 0.23458708814146836 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2900 0.22378373539811125 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2741 0.21151421335387 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2478 0.1912193435574206 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1857 0.1432987574601009 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1819 0.14036641885833254 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1679 0.12956306611497545 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1652 0.12747956237161373 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1559 0.12030304947781223 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1453 0.11212336811498472 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1414 0.10911386270790666 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1314 0.1013971821769373 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.858340265484677E-5 0.0 22-23 0.0 0.0 0.0 1.9291701327423384E-4 0.0 24-25 0.0 0.0 0.0 8.102514557517821E-4 0.0 26-27 0.0 0.0 0.0 0.002469337769910193 0.0 28-29 0.0 0.0 0.0 0.006829262269907878 0.0 30-31 0.0 0.0 0.0 0.016243612517690492 0.0 32-33 0.0 0.0 0.0 0.03565106405307841 0.0 34-35 0.0 0.0 0.0 0.06146336042917091 0.0 36-37 0.0 0.0 0.0 0.10193734981410517 0.0 38-39 0.0 0.0 0.0 0.15769036665035874 0.0 40-41 0.0 0.0 0.0 0.22308923415032403 0.0 42-43 0.0 0.0 0.0 0.2878321838051569 0.0 44-45 0.0 0.0 0.0 0.3626454015529048 0.0 46-47 0.0 0.0 0.0 0.4566345704201115 0.0 48-49 0.0 0.0 0.0 0.5472283998536918 0.0 50-51 0.0 0.0 0.0 0.6453074094023122 0.0 52-53 0.0 0.0 0.0 0.7393351616721737 0.0 54-55 0.0 0.0 0.0 0.8451308517517636 0.0 56-57 0.0 0.0 0.0 0.9775876730658526 0.0 58-59 0.0 0.0 0.0 1.1138642512427714 0.0 60-61 0.0 0.0 0.0 1.2579346767559692 0.0 62-63 0.0 0.0 0.0 1.4022366026850963 0.0 64-65 0.0 0.0 0.0 1.5542552091451922 0.0 66-67 0.0 0.0 0.0 1.7315459443442134 0.0 68-69 0.0 0.0 0.0 1.939356151043218 0.0 70-71 0.0 0.0 0.0 2.163680054078497 0.0 72-73 0.0 0.0 0.0 2.394331635149171 0.0 74-75 0.0 0.0 0.0 2.6349377341047955 0.0 76-77 0.0 0.0 0.0 2.8836077642152826 0.0 78-79 0.0 0.0 0.0 2.9530578889940076 3.858340265484677E-5 80-81 0.0 0.0 0.0 2.954639808502856 7.716680530969354E-5 82-83 0.0 0.0 0.0 2.954639808502856 7.716680530969354E-5 84-85 0.0 0.0 0.0 2.954639808502856 7.716680530969354E-5 86-87 0.0 0.0 0.0 2.954639808502856 7.716680530969354E-5 88-89 0.0 0.0 0.0 2.954639808502856 7.716680530969354E-5 90-91 0.0 0.0 0.0 2.954639808502856 7.716680530969354E-5 92-93 0.0 0.0 0.0 2.954639808502856 7.716680530969354E-5 94-95 0.0 0.0 0.0 2.954639808502856 7.716680530969354E-5 96-97 0.0 0.0 0.0 2.954639808502856 7.716680530969354E-5 98-99 0.0 0.0 0.0 2.954639808502856 7.716680530969354E-5 100-101 0.0 0.0 0.0 2.954639808502856 7.716680530969354E-5 102-103 0.0 0.0 0.0 2.954639808502856 7.716680530969354E-5 104-105 0.0 0.0 0.0 2.954639808502856 7.716680530969354E-5 106-107 0.0 0.0 0.0 2.954639808502856 7.716680530969354E-5 108-109 0.0 0.0 0.0 2.954639808502856 7.716680530969354E-5 110-111 0.0 0.0 0.0 2.954639808502856 7.716680530969354E-5 112-113 0.0 0.0 0.0 2.954639808502856 7.716680530969354E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATCG 5 2.1363462E-4 6183.44 110-111 CCGCTCC 750 0.0 896.91113 108-109 CGGTGCG 75 3.85861E-5 824.4587 110-111 CCGCGCC 750 0.0 736.7484 108-109 CCTCGCC 630 0.0 724.54553 108-109 CGACGCC 100 2.0160951E-7 720.7321 108-109 CCGTTCG 75 8.234917E-5 640.65076 108-109 CTCCGCC 320 0.0 579.6975 110-111 GTTGTCG 110 1.2163527E-4 562.1309 110-111 CGTCGCT 110 1.2163527E-4 562.1309 110-111 CGTCACT 200 1.1695629E-6 463.75803 110-111 CCGCGCT 105 2.25756E-4 457.6077 108-109 CCGCTCG 215 7.612471E-9 446.96567 108-109 CGTCGCG 110 1.3151111E-7 436.80734 108-109 CCGCACG 115 2.965038E-4 417.81573 108-109 CCGCTAC 120 3.3683187E-4 400.40674 108-109 TTTTTCG 640 0.0 384.11115 92-93 CGGCGCG 225 5.145748E-6 320.32538 108-109 TGTCGCT 195 6.7623466E-4 317.09952 110-111 CCCCTCG 595 0.0 316.75717 92-93 >>END_MODULE