FastQCFastQC Report
Fri 27 May 2016
SRR939974_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939974_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295894
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT135161.0429865405658179No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT78320.6043704191855198No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT60360.4657788368493102No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT41510.3203194088405379No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC41250.31831307190248587No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT40290.3109050585927553No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39350.3036513788936441No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC35790.2761799962033932No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC34010.2624443048582677No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG30110.23234925078748722No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA30020.2316547495397No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28490.2198482283273169No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC26890.20750153947776592No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA25200.19446034938042772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20040.15464227784062584No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA18970.14638542967248866No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC18160.14013491844240347No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC18040.13920891677868713No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT17240.13303557235391167No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG15330.11829671253976021No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA15150.11690771004418572No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC14950.11536437393799184No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG13410.10348068592029903No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAA1501.4613761E-731.7755991
CATGGGG24750.027.9229324
GTACATG67750.025.5904981
AGTACTT47450.025.3273512-13
GAGTACT45100.025.1300312-13
TACATGG67450.024.6433932
ACATGGG65350.024.2501433
GTACTTT47800.023.02821514-15
CTTAGCG1300.002036935922.9142113
CCTAATA3005.702532E-921.8448812
TACTTTT56300.020.87253214-15
ATGGGGA14550.019.2447875
ACTTTTT60000.018.69469816-17
ATGGGAG12450.018.6626595
GTATAGG3854.9385562E-918.5701561
ATGGGGG13050.018.2611335
CTGTACG1650.00809944918.0536214
GTGTAAG4651.4551915E-1017.937841
AGAGTAC104400.017.89384710-11
GTCCAAG11250.017.476581
Produced by FastQC (version 0.11.5)