FastQCFastQC Report
Fri 27 May 2016
SRR939973_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939973_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819803
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT88461.079039720518222No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT52590.6414955788158863No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT39480.4815791110791251No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT25940.31641748078501786No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT25720.3137339092440501No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC25490.3109283571784929No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC22830.2784815376377008No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG22630.27604192714591186No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC22520.27470014137542803No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA22290.27189458930987076No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC18100.22078474950689375No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA16100.1963886445890049No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA15150.18480049475300772No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC14260.1739442280645472No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA13780.16808916288425388No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG12540.15296357783516285No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT12410.15137783101550006No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC12380.15101188944173172No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11620.14174136957293398No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC10360.12637182347466402No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9800.11954091409765517No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA9600.11710130360586628No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC9080.1107583163272152No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG8470.10331750432725911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCG1150.01914.9576108-109
CGACGCG150.0030665591631.2603108-109
CCGCGCC2800.01573.0009108-109
CGTTGCA200.00368335961487.8336110-111
CCGTTCG551.7034836E-81334.6674108-109
CCGCGCG1350.01268.7579108-109
CGTCTAG250.005754611190.267110-111
CCGCTCC2850.01116.1254108-109
TTTCGCG300.008285717991.88916110-111
CGCTATG300.008285717991.88916110-111
GTGCGCG703.503587E-5850.1907110-111
GGTCGCC1153.2494972E-7638.3192108-109
CTCCGCC1951.5734258E-9610.3933110-111
CGTCTCG851.1298206E-4575.7389108-109
CGTCGAG151.2250079E-4562.030992-93
CTGTTCG1407.1315844E-7524.3336108-109
CCTCGCC4700.0520.615108-109
GGTCTCG1151.5517655E-4517.5074110-111
CGTCGCC1954.2109605E-9501.92624108-109
CAGTTCG1001.8388877E-4489.37805108-109
Produced by FastQC (version 0.11.5)