##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939973_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 819803 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.674351033114053 33.0 31.0 34.0 30.0 34.0 2 31.691566144549363 34.0 31.0 34.0 30.0 34.0 3 31.852898806176604 34.0 31.0 34.0 30.0 34.0 4 35.0966659063214 37.0 35.0 37.0 33.0 37.0 5 35.09992400613318 37.0 35.0 37.0 33.0 37.0 6 35.21152398808006 37.0 36.0 37.0 33.0 37.0 7 35.12488122146418 37.0 36.0 37.0 33.0 37.0 8 35.13070823112382 37.0 36.0 37.0 33.0 37.0 9 36.749725238868365 39.0 38.0 39.0 33.0 39.0 10-11 36.77275150249511 39.0 38.0 39.0 33.0 39.0 12-13 36.73985457481858 39.0 38.0 39.0 33.0 39.0 14-15 38.19331839478509 41.0 39.0 41.0 33.5 41.0 16-17 38.19177595105165 41.0 39.0 41.0 33.0 41.0 18-19 38.22165873996558 41.0 39.0 41.0 33.5 41.0 20-21 38.28598333989995 41.0 39.0 41.0 34.0 41.0 22-23 38.27397435725412 41.0 39.0 41.0 33.0 41.0 24-25 38.30781053496999 41.0 39.0 41.0 33.0 41.0 26-27 38.272258091273144 40.5 39.0 41.0 33.0 41.0 28-29 38.227474771378 40.0 38.0 41.0 33.0 41.0 30-31 38.16419676434461 40.0 38.0 41.0 33.0 41.0 32-33 38.03462051248897 40.0 38.0 41.0 32.5 41.0 34-35 37.89471007059013 40.0 38.0 41.0 32.0 41.0 36-37 38.07846153283167 40.0 38.0 41.0 33.0 41.0 38-39 38.04342262714335 40.0 38.0 41.0 33.0 41.0 40-41 38.01845809298088 40.0 38.0 41.0 33.0 41.0 42-43 37.88871777731967 40.0 38.0 41.0 32.0 41.0 44-45 37.72591890978686 40.0 38.0 41.0 32.0 41.0 46-47 37.594776427995505 40.0 38.0 41.0 31.5 41.0 48-49 37.461762764956944 40.0 38.0 41.0 31.0 41.0 50-51 37.29749098258972 40.0 37.0 41.0 31.0 41.0 52-53 37.17671440577797 40.0 37.0 41.0 30.5 41.0 54-55 36.983511892491244 40.0 37.0 41.0 30.0 41.0 56-57 36.73571028649566 40.0 36.0 41.0 30.0 41.0 58-59 36.52426375604871 39.5 36.0 41.0 30.0 41.0 60-61 36.218367095509535 39.0 35.0 41.0 30.0 41.0 62-63 35.95099676385668 39.0 35.0 41.0 30.0 41.0 64-65 35.62190428676158 38.5 35.0 40.5 29.0 41.0 66-67 35.253357818890635 37.5 35.0 40.0 29.0 41.0 68-69 34.830930113698045 37.0 34.0 40.0 28.5 41.0 70-71 34.38250286959184 36.5 34.0 39.0 28.0 41.0 72-73 33.96341438125989 36.0 34.0 39.0 27.5 41.0 74-75 33.55273035107214 35.5 34.0 38.5 27.0 40.0 76-77 33.098532818250234 35.0 33.5 37.0 26.0 39.5 78-79 32.62868030490252 35.0 33.0 37.0 26.0 39.0 80-81 32.157960510024964 35.0 33.0 36.5 25.0 39.0 82-83 32.00882529095405 35.0 33.0 36.0 25.0 37.5 84-85 31.611004107084263 35.0 33.0 36.0 22.0 37.0 86-87 30.909108651712668 35.0 33.0 35.0 2.0 37.0 88-89 15.614697067466208 18.0 16.0 18.5 2.0 19.0 90-91 2.7371325794123713 2.0 2.0 2.0 2.0 2.0 92-93 2.2815719142281745 2.0 2.0 2.0 2.0 2.0 94-95 2.096514040568283 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24375321736713929 1101 2 0.43797545973930596 1101 3 0.4238045011445202 1101 4 0.32419974836901133 1101 5 0.24635207484450206 1101 6 0.23318491273333564 1101 7 0.31862270925307 1101 8 0.3668475112157381 1101 9 0.3670001084669039 1101 10-11 0.2737423534734944 1101 12-13 0.42969783081129975 1101 14-15 0.5815117554766189 1101 16-17 0.5960893512978558 1101 18-19 0.5593190839339144 1101 20-21 0.4902930261617797 1101 22-23 0.49242495526666374 1101 24-25 0.3914301677792906 1101 26-27 0.45353513775179977 1101 28-29 0.5600445377350027 1101 30-31 0.458571025071457 1101 32-33 0.591781308528553 1101 34-35 0.4726520946327142 1101 36-37 0.4630061951386537 1101 38-39 0.4576809268622668 1101 40-41 0.45109390011997164 1101 42-43 0.5041277615438418 1101 44-45 0.39402150330323593 1101 46-47 0.465168130380917 1101 48-49 0.4852331926834168 1101 50-51 0.4230284327592173 1101 52-53 0.5063066350220637 1101 54-55 0.5408880377986662 1101 56-57 0.4982665105415123 1101 58-59 0.40267547131084314 1101 60-61 0.3922977440007642 1101 62-63 0.3667374983748246 1101 64-65 0.42923946795834667 1101 66-67 0.5628926783085149 1101 68-69 0.4847817229147324 1101 70-71 0.5251194341818461 1101 72-73 0.4619990188420857 1101 74-75 0.49308848363424573 1101 76-77 0.5174372496131028 1101 78-79 0.7100804661669073 1101 80-81 0.594479631753039 1101 82-83 0.6465254928271023 1101 84-85 0.7479348295077521 1101 86-87 0.9184439739049459 1101 88-89 1.0652781021753217 1101 90-91 0.023229248582719464 1101 92-93 -0.09396090925117839 1101 94-95 0.05486167863081137 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.2850015499354299 1102 2 0.26021183609057985 1102 3 0.4280119743192792 1102 4 0.44353548670623155 1102 5 0.3006525853173585 1102 6 0.38965162109221296 1102 7 0.5223882552618662 1102 8 0.378663556144744 1102 9 0.3901870364184532 1102 10-11 0.41718576553938647 1102 12-13 0.40227724369049866 1102 14-15 0.41102514268121837 1102 16-17 0.47983277784305756 1102 18-19 0.48611190034544904 1102 20-21 0.5330520595597008 1102 22-23 0.4652666131789971 1102 24-25 0.5641110128783495 1102 26-27 0.44929300664314553 1102 28-29 0.4987138579157744 1102 30-31 0.4259513627783704 1102 32-33 0.4792554691133901 1102 34-35 0.5083000901718577 1102 36-37 0.5243060679055347 1102 38-39 0.6554383274864151 1102 40-41 0.5591126677762617 1102 42-43 0.5872216984078662 1102 44-45 0.5830512659965095 1102 46-47 0.7147102605653544 1102 48-49 0.7170216036864545 1102 50-51 0.741528683836691 1102 52-53 0.8243220601131824 1102 54-55 0.7632156030248964 1102 56-57 0.74029595592215 1102 58-59 0.8722250105913716 1102 60-61 0.8881514998425502 1102 62-63 0.7389533110186548 1102 64-65 0.8333540578702667 1102 66-67 0.7162536507023134 1102 68-69 0.5352887299787952 1102 70-71 0.7182842734768542 1102 72-73 0.6593844243091098 1102 74-75 0.6390172546485076 1102 76-77 0.6089392012398704 1102 78-79 0.6641163440600693 1102 80-81 0.6159190296600059 1102 82-83 0.7206280063745041 1102 84-85 0.7676039771690277 1102 86-87 0.974900978042335 1102 88-89 1.4163225382676305 1102 90-91 0.1512063651042337 1102 92-93 -0.055298058551396245 1102 94-95 0.05406685668017008 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.2186985523737981 1103 2 0.3276033520885804 1103 3 0.21522528659962958 1103 4 0.2460473509715726 1103 5 0.22573513922903032 1103 6 0.20825189033033098 1103 7 0.30123820295880677 1103 8 0.2414977887685268 1103 9 0.48662669538350656 1103 10-11 0.3322223996383471 1103 12-13 0.36534667535504184 1103 14-15 0.4102920214881962 1103 16-17 0.48097985813208766 1103 18-19 0.5264811474669528 1103 20-21 0.6548120335582439 1103 22-23 0.5815440997335557 1103 24-25 0.5064220978318303 1103 26-27 0.4844012033260583 1103 28-29 0.46928433088326216 1103 30-31 0.40942630599166563 1103 32-33 0.44683526146770447 1103 34-35 0.48266646922662204 1103 36-37 0.4548531746938025 1103 38-39 0.41303142070151466 1103 40-41 0.49144957118653565 1103 42-43 0.4954076937800167 1103 44-45 0.5538709928493901 1103 46-47 0.5490900210034582 1103 48-49 0.5598661139153833 1103 50-51 0.590947128038593 1103 52-53 0.5526854686179732 1103 54-55 0.4754569334916283 1103 56-57 0.5223280082881558 1103 58-59 0.6576895195465866 1103 60-61 0.6417470145902584 1103 62-63 0.5855150348453506 1103 64-65 0.6459843122409907 1103 66-67 0.5607598535005494 1103 68-69 0.5851713500653659 1103 70-71 0.6474280918706938 1103 72-73 0.5812636448801243 1103 74-75 0.6770480537908696 1103 76-77 0.4909447543675398 1103 78-79 0.4703362716336983 1103 80-81 0.6852643154674638 1103 82-83 0.6961890472235908 1103 84-85 0.6523048395655131 1103 86-87 0.7864884654240853 1103 88-89 0.809399575652785 1103 90-91 -0.538982035696014 1103 92-93 -0.30420825907449967 1103 94-95 -0.10591570652819948 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 0.19776588465863298 1104 2 0.37919567617768024 1104 3 0.42560184776609233 1104 4 0.2332059830515405 1104 5 0.31077712767338994 1104 6 0.2622524463953866 1104 7 0.14289314661226626 1104 8 0.18586707682543846 1104 9 0.34420073018875286 1104 10-11 0.30951537260833106 1104 12-13 0.373354288743208 1104 14-15 0.37835701193687754 1104 16-17 0.318280687941602 1104 18-19 0.4652538206044454 1104 20-21 0.3785773824060641 1104 22-23 0.24493178081780087 1104 24-25 0.3679169445473818 1104 26-27 0.41902171659346266 1104 28-29 0.4346246717428386 1104 30-31 0.4334743904028997 1104 32-33 0.3942521871472593 1104 34-35 0.4222587158539213 1104 36-37 0.3099857796095833 1104 38-39 0.41777837652200844 1104 40-41 0.4681228497709782 1104 42-43 0.41424951540486177 1104 44-45 0.3917977416532281 1104 46-47 0.4192557477541925 1104 48-49 0.3915004748624611 1104 50-51 0.1690044138048492 1104 52-53 0.22009701346905075 1104 54-55 0.31545128984632953 1104 56-57 0.2655283148155547 1104 58-59 0.21681673256791356 1104 60-61 0.1862861653046366 1104 62-63 0.2537164133887089 1104 64-65 0.20571358325630484 1104 66-67 0.3739697182605468 1104 68-69 0.27826805143767785 1104 70-71 0.23829493088986453 1104 72-73 0.2976051103190258 1104 74-75 0.1581453001622677 1104 76-77 0.214561058416173 1104 78-79 0.2191079488337877 1104 80-81 0.24079820016281417 1104 82-83 0.29752152847714086 1104 84-85 0.45490693352391176 1104 86-87 0.6717941697938983 1104 88-89 0.5769273823634666 1104 90-91 -0.6141535236679951 1104 92-93 -0.30420825907449967 1104 94-95 -0.10591570652819948 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 0.11786473144611165 1105 2 0.10736701060930898 1105 3 0.12494286918289532 1105 4 0.04622081006966283 1105 5 -5.902528867380852E-4 1105 6 0.027491326131794835 1105 7 0.053931037880801114 1105 8 0.021946154255424233 1105 9 0.03365707285761488 1105 10-11 0.036862983810962646 1105 12-13 0.06734110917154368 1105 14-15 0.04227793450689887 1105 16-17 0.2733877719613744 1105 18-19 0.17736255207067586 1105 20-21 0.29908809080804133 1105 22-23 0.11354792579309247 1105 24-25 0.17145895443206172 1105 26-27 0.06976306749956507 1105 28-29 0.07136272775601782 1105 30-31 0.07433106256105049 1105 32-33 0.12324724480788518 1105 34-35 0.14672329575508058 1105 36-37 -0.12494008529980505 1105 38-39 -0.03362195296728032 1105 40-41 0.03525629293407917 1105 42-43 0.02256912001771383 1105 44-45 -0.01841642638630958 1105 46-47 -8.430990332755073E-4 1105 48-49 -0.00594598312765271 1105 50-51 0.07468810408491322 1105 52-53 0.04093721116262117 1105 54-55 0.05268357979690563 1105 56-57 -0.047898373817062634 1105 58-59 0.012533371777131208 1105 60-61 0.0759072526192952 1105 62-63 0.17958132277915695 1105 64-65 0.2501946376220374 1105 66-67 0.253294265212773 1105 68-69 0.24120050613289834 1105 70-71 0.127092294975526 1105 72-73 0.21482092580502155 1105 74-75 0.2882935703434839 1105 76-77 0.3764226729136979 1105 78-79 0.37396791588485456 1105 80-81 0.20784927100301331 1105 82-83 0.2052645268296942 1105 84-85 0.1447751378072688 1105 86-87 0.12443008412668277 1105 88-89 0.9224792769268948 1105 90-91 -0.7241205747388353 1105 92-93 -0.30420825907449967 1105 94-95 -0.10591570652819948 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 0.04641940374288467 1106 2 0.015533866545716535 1106 3 0.16657154861327328 1106 4 -0.015157233574335294 1106 5 -0.030109667696699205 1106 6 0.06628095948165225 1106 7 -0.11385925124871932 1106 8 0.030575254877611258 1106 9 -0.10447118271518008 1106 10-11 -0.08609703495606169 1106 12-13 0.004134346643752451 1106 14-15 0.005810187669837319 1106 16-17 -0.1301519720179769 1106 18-19 -0.07216627291080613 1106 20-21 0.08299203241699615 1106 22-23 0.06539343903397565 1106 24-25 -0.05950010998896005 1106 26-27 -0.10822512846659293 1106 28-29 0.015842049370618838 1106 30-31 0.08213444062602093 1106 32-33 0.18779089147593453 1106 34-35 0.020461053201906054 1106 36-37 0.12418954047926434 1106 38-39 0.10355928746925969 1106 40-41 0.1074500358364574 1106 42-43 -0.26687496010470113 1106 44-45 0.16208959025876624 1106 46-47 -0.010863065657829907 1106 48-49 0.029708703564288896 1106 50-51 0.27371656385576415 1106 52-53 0.11838452219944173 1106 54-55 0.06240964517227354 1106 56-57 -0.09762299967610488 1106 58-59 0.14211050052853835 1106 60-61 0.2639214131946801 1106 62-63 0.2513318812581744 1106 64-65 0.11144125875733835 1106 66-67 0.1641668786813142 1106 68-69 0.1444570119469546 1106 70-71 0.2656937248950726 1106 72-73 0.3473124086667809 1106 74-75 0.3322767919810161 1106 76-77 0.33160039986410794 1106 78-79 0.3155028934657267 1106 80-81 0.35248733927873843 1106 82-83 0.3768135530235597 1106 84-85 0.4196874339424319 1106 86-87 0.45170412005061067 1106 88-89 1.3874403833284763 1106 90-91 -0.29046361681003274 1106 92-93 -0.30420825907449967 1106 94-95 -0.10591570652819948 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 0.058123017763723084 1107 2 0.012518563980982833 1107 3 0.07529127745315378 1107 4 0.0628760126587693 1107 5 -0.16363674156330887 1107 6 -0.025531874647327868 1107 7 -0.12220465345508558 1107 8 -0.19433873045583283 1107 9 -0.21519511075660347 1107 10-11 -0.08042785745296044 1107 12-13 -0.16309042558021503 1107 14-15 -0.06326591136389226 1107 16-17 0.009572691649985643 1107 18-19 -0.05924268100984165 1107 20-21 -0.11436245784233989 1107 22-23 0.015033033111159 1107 24-25 -0.0201054565635701 1107 26-27 -0.11023086022343875 1107 28-29 -0.023709690973639397 1107 30-31 -0.08225989351701202 1107 32-33 0.006852942086020164 1107 34-35 -0.026168538397641328 1107 36-37 -0.18237771443320838 1107 38-39 -0.14377949409818314 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-1.7005955119979106 2216 64-65 -1.624034782583088 2216 66-67 -1.5703200197423257 2216 68-69 -1.5773093519741224 2216 70-71 -1.4279995103069254 2216 72-73 -1.51018837429595 2216 74-75 -1.5984936433462593 2216 76-77 -1.6432403977139245 2216 78-79 -1.6246696193226668 2216 80-81 -1.6209597846381598 2216 82-83 -1.6615646701730142 2216 84-85 -1.7638912277132697 2216 86-87 -1.910713668862634 2216 88-89 -2.2052336953853757 2216 90-91 -0.7241205747388353 2216 92-93 -0.30420825907449967 2216 94-95 -0.10591570652819948 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores warn #Quality Count 2 8119.0 3 1473.0 4 1273.0 5 1111.0 6 1535.0 7 1782.0 8 1713.0 9 1844.0 10 2310.0 11 2776.0 12 3224.0 13 3483.0 14 3875.0 15 4482.0 16 5103.0 17 6248.0 18 7663.0 19 7734.0 20 7773.0 21 10123.0 22 13270.0 23 18565.0 24 29458.0 25 51937.0 26 109013.0 27 265613.0 28 240143.0 29 7916.0 30 244.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.15353810610598 19.511516791229113 14.476282716701453 22.858662385963456 2 14.969992681141742 22.18309343742376 38.39363259331544 24.45328128811905 3 20.354815996251222 25.372833805388133 29.63645882018434 24.635891378176304 4 12.883605535073814 17.839373514972014 37.9697325820785 31.307288367875678 5 13.471261674544277 38.91258477125643 33.8513883753086 13.764765178890691 6 29.931825084806963 36.385936621359036 18.29866443523627 15.383573858597735 7 26.59212030207257 33.24286444426283 22.225217521770475 17.939797731894124 8 24.327551863069544 33.8360557353413 21.308411898956212 20.52798050263295 9 24.7844909081816 19.02298479024839 21.66496097233116 34.52756332923885 10-11 24.03764344243548 27.159267989960934 27.677261762813377 21.125826804790208 12-13 25.970513018344434 25.17164550970955 26.944487307960436 21.91335416398558 14-15 22.5468466776124 27.11032663984718 26.42095286422819 23.921873818312225 16-17 21.39001230180343 29.366362587879824 27.459003645407986 21.784621464908753 18-19 21.52821743951576 29.57503363793505 27.62842493952491 21.268323983024278 20-21 23.408432612836467 27.50678077024183 26.42368728740524 22.661099329516464 22-23 22.393863839296603 27.82509307193497 26.469267964225246 23.31177512454318 24-25 23.471248580451647 27.66780555816458 26.566992314007145 22.293953547376628 26-27 22.596709209407624 27.084128748004094 28.123646778555333 22.195515264032945 28-29 21.790845599155396 28.944005884423053 27.016808542172004 22.24833997424955 30-31 22.413616441998872 27.614744029968175 26.733373749547145 23.238265778485808 32-33 22.226078216229368 27.954607970441263 27.045962296510762 22.773351516818604 34-35 23.288320404390575 27.567283055715315 26.61138092574849 22.53301561414562 36-37 22.724234295884003 27.05613923378966 27.77265458324642 22.446971887079915 38-39 22.528705066949012 27.795885108983498 27.34461815826485 22.33079166580264 40-41 22.515470180031055 27.84199374727831 27.243496303380205 22.399039769310434 42-43 22.097503912525326 28.110838823473443 26.27942322728753 23.512234036713696 44-45 22.551536960234202 27.36246645523298 26.565259819468164 23.52073676506465 46-47 23.327067600386922 27.833150159245573 26.568212119253037 22.27157012111446 48-49 22.63921366282793 27.464713997665285 27.080230446169253 22.81584189333753 50-51 21.649814009854826 28.008548410804323 28.005071959492632 22.33656561984822 52-53 22.33756158491735 28.49324776806135 26.29918407227102 22.870006574750278 54-55 21.59804780225276 27.521243141569713 27.114189662281014 23.766519393896516 56-57 22.812004835307995 27.229285572265532 26.552354651056415 23.40635494137006 58-59 23.5849954196313 26.980567282627653 26.370908620729615 23.063528677011426 60-61 22.3959292659334 27.50801107095241 27.80039838839331 22.295661274720878 62-63 22.14446641449226 27.521916850755606 27.50282689865736 22.83078983609477 64-65 21.74119880019956 28.656274739175142 26.720626784727553 22.881899675897746 66-67 22.33182850026165 27.497886687411487 26.230874978500935 23.93940983382593 68-69 22.159381653684836 27.438050401348136 26.942197451937943 23.460370493029085 70-71 23.06700512196223 27.377003987543347 26.394572842499965 23.161418047994456 72-73 22.56328654567012 26.688118974924464 27.620538104886172 23.128056374519243 74-75 21.868729438657823 28.556555660323273 26.681227075285157 22.893487825733743 76-77 22.367080871867998 27.09504600495485 26.768077208792846 23.76979591438431 78-79 22.344853833162865 27.246601150647198 27.289965412274586 23.11857960391535 80-81 22.820543472029257 27.317233530494523 26.902804698201887 22.959418299274336 82-83 22.307412688750997 26.924775279733886 27.823290399940475 22.944521631574645 84-85 22.243453610196596 27.405852381608753 27.275638171609522 23.075055836585133 86-87 22.17702119250617 27.781413512776677 26.94718005548952 23.094385239227634 88-89 32.24570435997769 17.73754300157588 32.8107012089758 17.20605142947064 90-91 32.20571541792394 13.225541470421906 15.741712313782472 38.82703079787168 92-93 24.100000817902245 16.002973074658115 34.23779689851469 25.65922920892495 94-95 17.602720229833825 7.051679512441609 28.639642363034177 46.705957894690386 96-97 30.149782669449447 7.756233016405336 15.599636097682797 46.49434821646242 98-99 58.06246416284404 14.200473050458715 18.620090309633028 9.11697247706422 100-101 32.701167095677455 7.15516299445293 11.923789389161485 48.21988052070814 102-103 26.05703928893198 2.217740873239585 36.222134995442545 35.50308484238589 104-105 46.00304696104451 4.950341327562867 36.67900653544033 12.367605175952297 106-107 7.58931222167244 2.129308922975425 8.247732145802408 82.03364670954973 108-109 19.693154919243586 9.699766637048775 20.964655207285723 49.64242323642192 110-111 42.18434446233858 2.5817200478971523 24.643402524727996 30.59053296503627 112-113 30.202956268668146 3.0687243113527862 38.31967526997013 28.408644150008932 114-115 31.981575226889227 4.293793040543622 23.383955853513932 40.34067587905322 116-117 22.868706757458913 4.724660668544498 21.335630486504225 51.07100208749237 118-119 23.292593827048766 4.752991451104867 15.47784858324081 56.476566138605556 120-121 28.76310206647285 4.915219611848825 28.80961734894319 37.51206097273513 122-123 36.89960667999227 1.834547681991102 34.43884196273132 26.827003675285315 124-125 40.877083409873 1.9783974429626365 19.04307040425193 38.101448742912424 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 34.0 1 82.0 2 181.0 3 237.0 4 253.0 5 228.5 6 184.0 7 164.5 8 155.5 9 147.5 10 132.5 11 133.0 12 121.5 13 109.0 14 130.5 15 142.5 16 154.0 17 193.5 18 266.0 19 401.0 20 564.0 21 800.5 22 1131.5 23 1582.5 24 2237.0 25 3130.5 26 4162.5 27 5343.5 28 6883.5 29 8657.0 30 10491.0 31 12597.0 32 14866.0 33 17000.5 34 19150.0 35 21121.5 36 22688.5 37 24067.0 38 25389.5 39 26265.5 40 26907.0 41 28013.0 42 30088.0 43 34346.5 44 39249.0 45 40765.0 46 39000.5 47 36891.0 48 35951.5 49 35647.0 50 34573.0 51 31928.5 52 27854.5 53 23694.5 54 20503.0 55 17961.5 56 15651.0 57 13519.5 58 11461.5 59 9580.5 60 7924.5 61 6420.0 62 5062.0 63 3892.0 64 2940.0 65 2227.0 66 1716.5 67 1273.5 68 901.0 69 643.0 70 447.5 71 328.0 72 246.5 73 182.5 74 137.5 75 99.0 76 69.5 77 36.5 78 18.0 79 13.5 80 8.5 81 7.5 82 5.5 83 2.0 84 4.5 85 4.5 86 2.5 87 3.5 88 4.5 89 5.0 90 5.0 91 6.0 92 3.5 93 1.0 94 2.0 95 2.5 96 2.0 97 2.5 98 3.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 3.6594157376833216E-4 3 0.0409854562620532 4 0.045620716196452075 5 0.04781636563906207 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.708928852419423E-4 12-13 0.002134659180315271 14-15 1.2198052458944406E-4 16-17 5.489123606524982E-4 18-19 0.005367143081935539 20-21 0.002622581278673047 22-23 8.538636721261084E-4 24-25 0.0 26-27 0.0 28-29 0.0014027760327786065 30-31 0.0 32-33 0.0014027760327786065 34-35 1.8297078688416608E-4 36-37 2.4396104917888813E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 3.6594157376833216E-4 46-47 0.0 48-49 2.4396104917888813E-4 50-51 1.8297078688416608E-4 52-53 0.0 54-55 0.0010978247213049965 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 4.269318360630542E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 4.269318360630542E-4 80-81 0.0 82-83 7.318831475366643E-4 84-85 0.0 86-87 0.0010368344590102744 88-89 18.995844123527235 90-91 26.123410136337633 92-93 40.34469256638485 94-95 44.718792197637725 96-97 48.71420329030267 98-99 93.19251088371232 100-101 51.9956623725456 102-103 53.69643682689622 104-105 74.85896001844345 106-107 81.51080198535502 108-109 12.551064097106257 110-111 38.573169407772355 112-113 52.219008713068874 114-115 86.6266651866363 116-117 66.5465361800335 118-119 72.36891057973683 120-121 80.59442329437681 122-123 76.3525505517789 124-125 50.197181517998835 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 819803.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.95509140433064 #Duplication Level Percentage of deduplicated Percentage of total 1 70.35876614721182 35.14778593979891 2 14.69981507751543 14.686612116480818 3 6.061512418691589 9.084102207726708 4 3.0961476343037955 6.186733522917926 5 1.7286994595087863 4.31786697551892 6 1.096818189604772 3.28749917493833 7 0.725817951834614 2.538081148876153 8 0.4814309853878631 1.9239943103942134 9 0.3349151015890455 1.5057643061314308 >10 1.3415112019051605 10.57075979127797 >50 0.04180620390552892 1.4246461972951974 >100 0.02542538335223654 2.455742377617908 >500 0.0024447483964331 0.9514963622479293 >1k 0.004400547113579581 4.1963053532074 >5k 4.8894967928662E-4 1.7226102155701504 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8846 1.079039720518222 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5259 0.6414955788158863 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3948 0.4815791110791251 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2594 0.31641748078501786 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2572 0.3137339092440501 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2549 0.3109283571784929 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2283 0.2784815376377008 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2263 0.27604192714591186 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2252 0.27470014137542803 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2229 0.27189458930987076 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1810 0.22078474950689375 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1610 0.1963886445890049 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1515 0.18480049475300772 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1426 0.1739442280645472 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1378 0.16808916288425388 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1254 0.15296357783516285 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1241 0.15137783101550006 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1238 0.15101188944173172 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1162 0.14174136957293398 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1036 0.12637182347466402 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 980 0.11954091409765517 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 960 0.11710130360586628 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 908 0.1107583163272152 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 847 0.10331750432725911 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.2198052458944405E-4 0.0 0.0 0.0 0.0 3 1.2198052458944405E-4 0.0 0.0 0.0 0.0 4 1.2198052458944405E-4 0.0 0.0 0.0 0.0 5 1.2198052458944405E-4 0.0 0.0 1.2198052458944405E-4 0.0 6 1.2198052458944405E-4 0.0 0.0 1.2198052458944405E-4 0.0 7 2.439610491788881E-4 0.0 0.0 1.2198052458944405E-4 0.0 8 2.439610491788881E-4 0.0 0.0 1.2198052458944405E-4 0.0 9 2.439610491788881E-4 0.0 0.0 1.2198052458944405E-4 0.0 10-11 2.439610491788881E-4 0.0 0.0 1.2198052458944405E-4 0.0 12-13 4.879220983577762E-4 0.0 0.0 1.2198052458944405E-4 0.0 14-15 4.879220983577762E-4 0.0 0.0 1.2198052458944405E-4 0.0 16-17 4.879220983577762E-4 0.0 0.0 2.439610491788881E-4 0.0 18-19 4.879220983577762E-4 0.0 0.0 2.439610491788881E-4 0.0 20-21 4.879220983577762E-4 0.0 0.0 3.6594157376833216E-4 0.0 22-23 4.879220983577762E-4 0.0 0.0 7.318831475366643E-4 0.0 24-25 4.879220983577762E-4 0.0 0.0 0.0013417857704838844 0.0 26-27 4.879220983577762E-4 0.0 0.0 0.0028665423278519354 0.0 28-29 4.879220983577762E-4 0.0 0.0 0.010673295901576355 0.0 30-31 4.879220983577762E-4 0.0 0.0 0.02439610491788881 0.0 32-33 4.879220983577762E-4 0.0 0.0 0.051963703475103165 0.0 34-35 4.879220983577762E-4 0.0 0.0 0.09648659495025025 0.0 36-37 6.099026229472202E-4 0.0 0.0 0.15808675986791948 0.0 38-39 6.099026229472202E-4 0.0 0.0 0.2414604484248045 0.0 40-41 6.708928852419422E-4 0.0 0.0 0.33636129655539193 0.0 42-43 7.318831475366643E-4 0.0 0.0 0.42821263157124334 0.0 44-45 7.318831475366643E-4 0.0 0.0 0.5359824250460171 0.0 46-47 8.538636721261083E-4 0.0 0.0 0.6685752552747428 0.0 48-49 8.538636721261083E-4 0.0 0.0 0.791165682487134 0.0 50-51 8.538636721261083E-4 0.0 0.0 0.9160127494044301 0.0 52-53 8.538636721261083E-4 0.0 0.0 1.0361635661250326 0.0 54-55 8.538636721261083E-4 0.0 0.0 1.1816253416979445 0.0 56-57 0.0010978247213049965 0.0 0.0 1.3680725735329098 0.0 58-59 0.0010978247213049965 0.0 0.0 1.5391502592696051 0.0 60-61 0.0012198052458944405 0.0 0.0 1.7127285457603838 0.0 62-63 0.0013417857704838844 0.0 0.0 1.889173374579015 0.0 64-65 0.0013417857704838844 0.0 0.0 2.0811707202828 0.0 66-67 0.0015857468196627726 0.0 0.0 2.3203745290026996 0.0 68-69 0.0017077273442522166 0.0 0.0 2.596294475624022 0.0 70-71 0.0018297078688416608 0.0 0.0 2.877337604278101 0.0 72-73 0.0018297078688416608 0.0 0.0 3.1486222909650246 0.0 74-75 0.0018297078688416608 0.0 0.0 3.455403310307476 0.0 76-77 0.0019516883934311048 0.0 0.0 3.783957853289144 0.0 78-79 0.0019516883934311048 0.0 0.0 3.8750773051574585 0.0 80-81 0.0019516883934311048 0.0 0.0 3.876297110403353 0.0 82-83 0.0019516883934311048 0.0 0.0 3.876297110403353 0.0 84-85 0.0019516883934311048 0.0 0.0 3.876297110403353 0.0 86-87 0.0019516883934311048 0.0 0.0 3.876297110403353 0.0 88-89 0.0019516883934311048 0.0 0.0 3.876297110403353 0.0 90-91 0.0019516883934311048 0.0 0.0 3.876297110403353 0.0 92-93 0.0019516883934311048 0.0 0.0 3.876297110403353 0.0 94-95 0.0019516883934311048 0.0 0.0 3.876297110403353 0.0 96-97 0.0019516883934311048 0.0 0.0 3.876297110403353 0.0 98-99 0.0019516883934311048 0.0 0.0 3.876297110403353 0.0 100-101 0.0019516883934311048 0.0 0.0 3.876297110403353 0.0 102-103 0.0019516883934311048 0.0 0.0 3.876297110403353 0.0 104-105 0.0019516883934311048 0.0 0.0 3.876297110403353 0.0 106-107 0.0019516883934311048 0.0 0.0 3.876297110403353 0.0 108-109 0.0019516883934311048 0.0 0.0 3.876297110403353 0.0 110-111 0.0019516883934311048 0.0 0.0 3.876297110403353 0.0 112-113 0.0019516883934311048 0.0 0.0 3.876297110403353 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCTCG 115 0.0 1914.9576 108-109 CGACGCG 15 0.003066559 1631.2603 108-109 CCGCGCC 280 0.0 1573.0009 108-109 CGTTGCA 20 0.0036833596 1487.8336 110-111 CCGTTCG 55 1.7034836E-8 1334.6674 108-109 CCGCGCG 135 0.0 1268.7579 108-109 CGTCTAG 25 0.00575461 1190.267 110-111 CCGCTCC 285 0.0 1116.1254 108-109 TTTCGCG 30 0.008285717 991.88916 110-111 CGCTATG 30 0.008285717 991.88916 110-111 GTGCGCG 70 3.503587E-5 850.1907 110-111 GGTCGCC 115 3.2494972E-7 638.3192 108-109 CTCCGCC 195 1.5734258E-9 610.3933 110-111 CGTCTCG 85 1.1298206E-4 575.7389 108-109 CGTCGAG 15 1.2250079E-4 562.0309 92-93 CTGTTCG 140 7.1315844E-7 524.3336 108-109 CCTCGCC 470 0.0 520.615 108-109 GGTCTCG 115 1.5517655E-4 517.5074 110-111 CGTCGCC 195 4.2109605E-9 501.92624 108-109 CAGTTCG 100 1.8388877E-4 489.37805 108-109 >>END_MODULE