FastQCFastQC Report
Fri 27 May 2016
SRR939972_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939972_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016150
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA30790.3030064458987354No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC26210.25793436008463316No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA20290.19967524479653592No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19380.19071987403434532No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA17690.17408847119027704No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA16980.16710131378241402No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA11360.11179451852580821No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10780.10608669979825813No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTCG52.2809466E-45982.2686110-111
CGTCGCT450.03988.1787110-111
CGACGCG100.00129784562508.415108-109
CCGCGCT301.371518E-92508.4148108-109
CGTGTCG251.5769638E-62392.9072110-111
CGTAGCG150.0020523981994.0894110-111
CCGCTCG404.3328328E-91881.3113108-109
CTCCGCC1150.01820.6903110-111
CCGCGCG456.9394446E-91672.2767108-109
CCGCTAT150.0029197671672.2766108-109
CCGAACG150.0029197671672.2766108-109
CCGCTCC2550.01573.9075108-109
CGGCGTG406.4568376E-61495.5671110-111
CCGCACG357.3461615E-61433.3799108-109
CCGCGCC2000.01379.6282108-109
CGTTGGT459.192287E-61329.3928110-111
TCGTTCG200.00519001061254.2075108-109
CGACGCC401.0964071E-51254.2075108-109
CAGTTCG602.1920641E-81254.2074108-109
CTTATCG250.00569984271196.4536110-111
Produced by FastQC (version 0.11.5)