##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939972_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1016150 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.817346848398365 34.0 31.0 34.0 30.0 34.0 2 31.963371549475962 34.0 31.0 34.0 30.0 34.0 3 32.125101609014415 34.0 31.0 34.0 30.0 34.0 4 35.34674113073857 37.0 35.0 37.0 33.0 37.0 5 35.40119175318605 37.0 37.0 37.0 33.0 37.0 6 35.463523101904244 37.0 37.0 37.0 35.0 37.0 7 35.40645869212223 37.0 37.0 37.0 35.0 37.0 8 35.4166687988978 37.0 37.0 37.0 35.0 37.0 9 37.06921911135167 39.0 39.0 39.0 35.0 39.0 10-11 37.04555872656596 39.0 39.0 39.0 34.5 39.0 12-13 36.98754662205383 39.0 38.0 39.0 34.0 39.0 14-15 38.50769620626876 41.0 39.5 41.0 34.5 41.0 16-17 38.51402598041628 41.0 39.0 41.0 34.0 41.0 18-19 38.525039118240414 41.0 39.5 41.0 34.0 41.0 20-21 38.56446292378094 41.0 39.5 41.0 34.0 41.0 22-23 38.55197461004773 41.0 39.0 41.0 34.0 41.0 24-25 38.567601732027754 41.0 39.0 41.0 34.0 41.0 26-27 38.52085764896915 41.0 39.0 41.0 34.0 41.0 28-29 38.46098902721055 41.0 39.0 41.0 34.0 41.0 30-31 38.359661467303056 41.0 39.0 41.0 34.0 41.0 32-33 38.26151995276288 40.5 39.0 41.0 33.0 41.0 34-35 38.219527628794964 40.0 38.5 41.0 33.0 41.0 36-37 38.37995620725286 41.0 39.0 41.0 34.0 41.0 38-39 38.31599911430399 41.0 39.0 41.0 33.0 41.0 40-41 38.19489888303892 41.0 39.0 41.0 33.0 41.0 42-43 38.168932244255274 41.0 39.0 41.0 33.0 41.0 44-45 38.11619150715938 41.0 39.0 41.0 33.0 41.0 46-47 37.99946858239433 41.0 38.0 41.0 33.0 41.0 48-49 37.906515278256165 40.0 38.0 41.0 33.0 41.0 50-51 37.798038183339074 40.0 38.0 41.0 33.0 41.0 52-53 37.71181420065935 40.0 38.0 41.0 32.5 41.0 54-55 37.57744181469271 40.0 38.0 41.0 32.0 41.0 56-57 37.36088717216947 40.0 38.0 41.0 31.0 41.0 58-59 37.19727845298431 40.0 37.0 41.0 31.0 41.0 60-61 36.98985139989175 40.0 37.0 41.0 31.0 41.0 62-63 36.74167888599124 40.0 36.0 41.0 31.0 41.0 64-65 36.454395020420215 39.5 36.0 41.0 30.0 41.0 66-67 36.13262608866801 39.0 35.0 41.0 30.0 41.0 68-69 35.69258229592088 39.0 35.0 41.0 29.0 41.0 70-71 35.2426526595483 38.0 35.0 40.0 28.5 41.0 72-73 34.853186045367316 37.0 35.0 40.0 28.0 41.0 74-75 34.431869310633274 37.0 34.0 39.0 28.0 41.0 76-77 33.92421296068494 36.0 34.0 39.0 26.5 40.5 78-79 33.42066082763371 36.0 34.0 38.5 26.0 40.0 80-81 32.94197116567436 35.0 34.0 37.0 26.0 39.0 82-83 32.70115140481228 35.0 34.0 37.0 26.0 39.0 84-85 32.20263642178812 35.0 34.0 37.0 24.0 39.0 86-87 31.494655808689664 35.0 34.0 36.0 4.5 37.0 88-89 15.926714067804951 18.0 16.5 18.5 2.0 19.5 90-91 2.762594105201004 2.0 2.0 2.0 2.0 2.0 92-93 2.3022329380504845 2.0 2.0 2.0 2.0 2.0 94-95 2.102089258475619 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.1240039413213978 1101 2 -0.07999971776872172 1101 3 -0.10102416209711151 1101 4 -0.01715560814663064 1101 5 -0.16953202301910864 1101 6 -0.12399431395947857 1101 7 -0.16689839998015543 1101 8 -0.09097215371569689 1101 9 -0.0641045280346475 1101 10-11 -0.09972293280404187 1101 12-13 -0.027448896732202854 1101 14-15 -0.17390963527225267 1101 16-17 -0.13627320444818736 1101 18-19 -0.13908982821998706 1101 20-21 -0.17924389970363563 1101 22-23 -0.14610609571546718 1101 24-25 -0.23241991109190252 1101 26-27 -0.288702523872594 1101 28-29 -0.09817354220871977 1101 30-31 -0.1469626470124581 1101 32-33 -0.16327993342564184 1101 34-35 -0.20437019143910362 1101 36-37 -0.12006541046621066 1101 38-39 -0.15556326276833943 1101 40-41 -0.20850184855671472 1101 42-43 -0.25452802266177343 1101 44-45 -0.3578200450221516 1101 46-47 -0.19496404960688096 1101 48-49 -0.22370696599096362 1101 50-51 -0.3446720554188758 1101 52-53 -0.3124059146419853 1101 54-55 -0.2990413559057359 1101 56-57 -0.22777405252558225 1101 58-59 -0.11924296756482988 1101 60-61 -0.025447065174233785 1101 62-63 -0.17730047163103535 1101 64-65 -0.12812278284454948 1101 66-67 -0.013743754915871875 1101 68-69 0.007625293614751172 1101 70-71 0.07229000124517881 1101 72-73 -0.0871475968484674 1101 74-75 -0.07876327438001596 1101 76-77 -0.05005775397216894 1101 78-79 -0.13357815807477635 1101 80-81 -0.1823942968006378 1101 82-83 -0.1622794650892203 1101 84-85 -0.14678650411590866 1101 86-87 -0.0617501931660378 1101 88-89 0.39476517389170596 1101 90-91 -0.002830556940570439 1101 92-93 -0.09596335346628582 1101 94-95 0.04347836898009305 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.04118994253366992 1102 2 -0.0030646448853417496 1102 3 0.14627618098734985 1102 4 0.14255521225483392 1102 5 0.11511332886315273 1102 6 0.08867391806683855 1102 7 0.1532117636764312 1102 8 0.1622600785695525 1102 9 0.28447634986142134 1102 10-11 0.1977071703566864 1102 12-13 0.11995089965334671 1102 14-15 0.17783285718047637 1102 16-17 0.18750319628387757 1102 18-19 0.021870043255745486 1102 20-21 0.0817857067790726 1102 22-23 0.1834153514771799 1102 24-25 0.19192937596327653 1102 26-27 0.1447152564945 1102 28-29 0.06542419567004742 1102 30-31 0.09908864499773529 1102 32-33 0.10155126077900434 1102 34-35 0.14496394222847897 1102 36-37 0.1315073051973883 1102 38-39 0.17005508371754274 1102 40-41 0.065926000721511 1102 42-43 0.09899315382958207 1102 44-45 0.1524842585399213 1102 46-47 0.06983919174685838 1102 48-49 0.07251086616246027 1102 50-51 0.03887489654341181 1102 52-53 0.08973409677454924 1102 54-55 0.05844912541292757 1102 56-57 0.07890255947118874 1102 58-59 0.07183389394791106 1102 60-61 0.035153244366810554 1102 62-63 0.1255730814411251 1102 64-65 0.07171509062025194 1102 66-67 0.048605814610226616 1102 68-69 0.018784365933193214 1102 70-71 0.1279956194739711 1102 72-73 0.018139022857411646 1102 74-75 0.14010019212658165 1102 76-77 0.045289420435963734 1102 78-79 0.03815533061755616 1102 80-81 -0.01006791342876312 1102 82-83 0.16905303260098492 1102 84-85 0.05828737968261777 1102 86-87 0.23116184656820238 1102 88-89 0.488520507001418 1102 90-91 -0.03768629080589081 1102 92-93 -9.236604180129504E-4 1102 94-95 0.10839371597795644 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.12567027435643396 1103 2 0.1691836686660224 1103 3 0.3265369045236284 1103 4 0.3121649808484648 1103 5 0.2724582752098925 1103 6 0.17727437917178435 1103 7 0.18082398011112133 1103 8 0.28218436627626886 1103 9 0.36954167306182484 1103 10-11 0.37208595952925805 1103 12-13 0.3851260910688268 1103 14-15 0.372073314869823 1103 16-17 0.44990464221790205 1103 18-19 0.295444745103012 1103 20-21 0.3338621021907997 1103 22-23 0.422353102309728 1103 24-25 0.40095934778464226 1103 26-27 0.3753004612877149 1103 28-29 0.3092391275005255 1103 30-31 0.2954524624912551 1103 32-33 0.3381294066817162 1103 34-35 0.37609279544341234 1103 36-37 0.33607139024563537 1103 38-39 0.2864413871929088 1103 40-41 0.3507621946132886 1103 42-43 0.36132059526640603 1103 44-45 0.31095919663231086 1103 46-47 0.30157431257343603 1103 48-49 0.3120790783845919 1103 50-51 0.2944608129183308 1103 52-53 0.27954993942960726 1103 54-55 0.28127524330778897 1103 56-57 0.35018312190536705 1103 58-59 0.27804742538559424 1103 60-61 0.3069361834838773 1103 62-63 0.28513673954034857 1103 64-65 0.4016527560629939 1103 66-67 0.3356692592349333 1103 68-69 0.37633851730084444 1103 70-71 0.4155059374086747 1103 72-73 0.4165422335140576 1103 74-75 0.35382233370287963 1103 76-77 0.3355962544666511 1103 78-79 0.4341519719519198 1103 80-81 0.48254729164908383 1103 82-83 0.42106965514958716 1103 84-85 0.3438963513047426 1103 86-87 0.46351746664050353 1103 88-89 0.7823710179170824 1103 90-91 -0.5524444953429355 1103 92-93 -0.3220533675309838 1103 94-95 -0.11580433144184799 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.09984883859431903 1104 2 0.03779566685683733 1104 3 0.12928266367349295 1104 4 0.1519565589975187 1104 5 0.1671120400066073 1104 6 0.2040635770537591 1104 7 0.1906986620188107 1104 8 0.1403378089831051 1104 9 0.15701443248124036 1104 10-11 0.2326304056488766 1104 12-13 0.2113510605730866 1104 14-15 0.26705830685184395 1104 16-17 0.29135098569820883 1104 18-19 0.2510043363001415 1104 20-21 0.11850640988028971 1104 22-23 0.04145051965141988 1104 24-25 0.17104610646394036 1104 26-27 0.2051643611794347 1104 28-29 0.20836011908049556 1104 30-31 0.23694243658700742 1104 32-33 0.21490741136915403 1104 34-35 0.2813207025901505 1104 36-37 0.1766999452270568 1104 38-39 0.17518217565066863 1104 40-41 0.19691447688281727 1104 42-43 0.18892438359168295 1104 44-45 0.22646565442026656 1104 46-47 0.2529465068507406 1104 48-49 0.162850951942616 1104 50-51 0.028952527670050188 1104 52-53 0.044226501793971806 1104 54-55 0.1316151509296688 1104 56-57 0.10960984759900327 1104 58-59 0.008990897468848402 1104 60-61 0.06772825047857367 1104 62-63 0.020958160116641977 1104 64-65 -0.16536683888042347 1104 66-67 -0.07074946675109572 1104 68-69 0.06709705039843783 1104 70-71 0.07531688663725333 1104 72-73 0.21194731294005464 1104 74-75 0.14154476989024545 1104 76-77 0.09457319648590357 1104 78-79 0.16269507488819102 1104 80-81 0.00169313668958182 1104 82-83 0.1796594040108559 1104 84-85 0.12442955854330506 1104 86-87 -0.018437961601986075 1104 88-89 0.7217365693760804 1104 90-91 -0.6429620321091529 1104 92-93 -0.3220533675309838 1104 94-95 -0.11580433144184799 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.20372746299937816 1105 2 -0.08643966762501876 1105 3 -0.05995562727169812 1105 4 -0.10924880205600118 1105 5 -0.051151603524211 1105 6 -0.11774928246678229 1105 7 -0.015636016931175334 1105 8 -0.27242872896182746 1105 9 -0.18541063394793156 1105 10-11 -0.10743701814006812 1105 12-13 -0.02843590981848365 1105 14-15 -0.13796439586664633 1105 16-17 -0.05991632421215343 1105 18-19 0.1258457913911002 1105 20-21 0.030324872081358478 1105 22-23 -0.1187234098168588 1105 24-25 0.014457573161756443 1105 26-27 -0.029471610584515417 1105 28-29 0.00791555459309734 1105 30-31 -0.06754249092489317 1105 32-33 -0.10426744336265159 1105 34-35 -0.05506893555332937 1105 36-37 -0.13128802634709302 1105 38-39 -0.1335503866905583 1105 40-41 -0.15848617864905634 1105 42-43 -0.13125648991548644 1105 44-45 -0.15498837804973675 1105 46-47 -0.07792417160284515 1105 48-49 -0.004797400467822399 1105 50-51 -0.09799426693970048 1105 52-53 -0.07404320978660195 1105 54-55 -0.19951307745530045 1105 56-57 -3.21998621103603E-4 1105 58-59 -0.13327087911011404 1105 60-61 -0.21894558226590277 1105 62-63 -0.18839457515944247 1105 64-65 -0.11513730235167685 1105 66-67 -0.15615407335897658 1105 68-69 -0.1475431005636878 1105 70-71 -0.2892310117187975 1105 72-73 -0.2757769767787579 1105 74-75 -0.24206091089931192 1105 76-77 -0.23747892669276638 1105 78-79 -0.22770796223915113 1105 80-81 -0.23613061429590232 1105 82-83 -0.17187298386253502 1105 84-85 -0.18744561482953515 1105 86-87 -0.16145619130093536 1105 88-89 0.7898771181832736 1105 90-91 -0.7514240871796702 1105 92-93 -0.3220533675309838 1105 94-95 -0.11580433144184799 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.04228584342705943 1106 2 -0.07540503552891664 1106 3 0.038510315703909725 1106 4 -0.06490438380137675 1106 5 0.012961792096724878 1106 6 -0.04603216094916007 1106 7 -0.06232953211022618 1106 8 -0.02899102484686722 1106 9 -0.014611674756721982 1106 10-11 -0.0992815162657621 1106 12-13 -0.0036914084979500217 1106 14-15 0.01795635783227567 1106 16-17 -0.02636714670668283 1106 18-19 -0.13029962187957267 1106 20-21 -0.11460784097915422 1106 22-23 -0.1017625661653625 1106 24-25 -0.10843560549385955 1106 26-27 -0.14896272537166766 1106 28-29 -0.0586401208676719 1106 30-31 0.030252313195745728 1106 32-33 0.08349258134413162 1106 34-35 -0.15897387655524398 1106 36-37 -0.04787267725726707 1106 38-39 -0.023127966348639006 1106 40-41 0.020282138449744025 1106 42-43 -0.3449111593557532 1106 44-45 -0.11894433891645662 1106 46-47 -0.3114001783333151 1106 48-49 -0.2711909376557884 1106 50-51 -0.23128266540049225 1106 52-53 -0.33551735463368004 1106 54-55 -0.2369954614470018 1106 56-57 -0.3709324177134832 1106 58-59 -0.08073546072770199 1106 60-61 -0.04658292500863581 1106 62-63 -0.054942884732618325 1106 64-65 -0.04302814699712343 1106 66-67 -0.07445506482518738 1106 68-69 -0.08492151904550127 1106 70-71 -0.05044446626683907 1106 72-73 0.04579548935591049 1106 74-75 0.033754306491992736 1106 76-77 -0.056816112178239564 1106 78-79 0.05034164834501098 1106 80-81 0.01829956773913466 1106 82-83 -0.017207759016599766 1106 84-85 -0.09087106317247873 1106 86-87 0.03352573121347291 1106 88-89 0.22603129064798821 1106 90-91 -0.3170674575258494 1106 92-93 -0.30311149947089255 1106 94-95 -0.11580433144184799 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 0.01847364163698373 1107 2 -0.01643634381522574 1107 3 0.08208685384623493 1107 4 0.1454398913331545 1107 5 0.18244504154947805 1107 6 0.1566453598347053 1107 7 0.14449647578473446 1107 8 0.07556045451246973 1107 9 0.08404514523794404 1107 10-11 0.18943334090211295 1107 12-13 0.18534119337520139 1107 14-15 0.18633512889927317 1107 16-17 0.22476378561501065 1107 18-19 0.1651496211978838 1107 20-21 0.1262967858886057 1107 22-23 0.2858436904773356 1107 24-25 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2215 118-119 0.0 2215 120-121 0.0 2215 122-123 0.0 2215 124-125 0.0 2216 1 0.2362663454761318 2216 2 0.11736468020107083 2216 3 0.09202910030122524 2216 4 0.061086916160839166 2216 5 0.10411807176472365 2216 6 0.1259903983257331 2216 7 0.07058096160487537 2216 8 0.13895507676189567 2216 9 0.032513829453279186 2216 10-11 0.04113807129306224 2216 12-13 -0.00668818004412941 2216 14-15 0.03687737628526122 2216 16-17 0.003043740454884869 2216 18-19 0.0017100250179566956 2216 20-21 -0.0888017789918365 2216 22-23 -0.13233186468752223 2216 24-25 -0.16671331291420444 2216 26-27 -0.15004295070558982 2216 28-29 -0.08543887578665732 2216 30-31 -0.19244359851983006 2216 32-33 -0.1974326882718458 2216 34-35 -0.26727913780980117 2216 36-37 -0.3216645054334961 2216 38-39 -0.37483495603563455 2216 40-41 -0.38573232614492525 2216 42-43 -0.23292648166012242 2216 44-45 -0.27835450353129687 2216 46-47 -0.28311301309982184 2216 48-49 -0.3069179976059573 2216 50-51 -0.3371504997410213 2216 52-53 -0.45303760301720075 2216 54-55 -0.35080555819278203 2216 56-57 -0.5050473357152114 2216 58-59 -0.43365010998518017 2216 60-61 -0.4199465453919373 2216 62-63 -0.5723808850108512 2216 64-65 -0.6770740456097926 2216 66-67 -0.620161537585723 2216 68-69 -0.7302515485548824 2216 70-71 -0.6776029847512746 2216 72-73 -0.705933140785362 2216 74-75 -0.6467630297765865 2216 76-77 -0.6318317403966773 2216 78-79 -0.6175719206871406 2216 80-81 -0.6482109665248572 2216 82-83 -0.7047181613529077 2216 84-85 -0.8077202777354273 2216 86-87 -0.7958509697819522 2216 88-89 -1.2785822241392335 2216 90-91 -0.7514240871796702 2216 92-93 -0.3220533675309838 2216 94-95 -0.11580433144184799 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11619.0 3 2067.0 4 1412.0 5 1404.0 6 1293.0 7 1185.0 8 1363.0 9 1658.0 10 1925.0 11 2367.0 12 2762.0 13 3145.0 14 3822.0 15 4320.0 16 5167.0 17 6020.0 18 7485.0 19 9390.0 20 10559.0 21 14463.0 22 17389.0 23 21660.0 24 32781.0 25 55525.0 26 97927.0 27 261312.0 28 420201.0 29 15311.0 30 618.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.08453476356837 20.86001082517345 15.307090488608965 23.748363922649215 2 12.59853445679836 20.326489801622213 46.09345937870888 20.981516362870543 3 18.716520681001715 26.532800256758872 29.4347268343541 25.31595222788531 4 11.221227715909661 18.260770253945342 40.14212984067005 30.37587218947495 5 11.751350574344848 39.08892474886259 34.890157224871245 14.269567451921326 6 28.184421591300495 37.724154898390985 20.84524922501599 13.246174285292525 7 27.27510702160114 31.91074152438124 23.191359543374503 17.622791910643112 8 23.41898341780249 40.08689661959357 21.06608276337155 15.428037199232397 9 25.193918220735128 18.92958716724893 22.840525512965606 33.03596909905034 10-11 23.33976124388188 26.640925501025205 31.376092798099474 18.64322045699344 12-13 26.506501334711103 24.688243470986947 29.803348464159622 19.00190673014233 14-15 19.856280990149582 31.34002427792367 25.912574872102972 22.891119859823775 16-17 19.24137869951759 30.97438753879856 31.03899439647452 18.74523936520933 18-19 20.918814995465418 28.454438403256432 31.65172444506119 18.97502215621695 20-21 21.592729713345456 29.06313721785273 28.719826873568106 20.624306195233707 22-23 20.098716275096702 31.840107386301852 29.17144166011822 18.88973467848323 24-25 21.17669635388476 29.227181026423267 31.128376716036016 18.467745903655956 26-27 18.7086060128918 31.365448014564777 30.347586478374254 19.578359494169167 28-29 19.05904651871126 31.338315260847548 27.969191238014073 21.633446982427117 30-31 20.206514786202824 29.128229100034442 29.931604585937116 20.733651527825618 32-33 18.96054575210675 30.75952207325966 31.09520449466067 19.18472767997292 34-35 19.35238796297982 31.037638691588874 30.76661531262409 18.84335803280721 36-37 18.64784660131211 30.362953482730642 30.225178480135057 20.764021435822187 38-39 20.49790877331103 31.076219062146336 28.838311272941986 19.58756089160065 40-41 22.36913841460414 28.096540865029766 30.914973183093046 18.61934753727304 42-43 19.10303596909905 31.371746297298625 30.79717561383654 18.728042119765785 44-45 18.587735946074808 31.466808247022946 29.524129593263186 20.42132621363905 46-47 21.047433941839294 29.058505141957387 29.537715888402303 20.356345027801016 48-49 19.959533473699956 29.882492568753484 29.264472663198344 20.89350129434822 50-51 18.49954165208958 31.67073348951114 29.72284097959995 20.10688387879933 52-53 18.668946513802094 30.428234020567828 30.63302662008562 20.26979284554446 54-55 19.095953624112628 29.37060803645985 30.72988092584474 20.80355741358279 56-57 19.972445013039415 29.875166068001775 28.03587068838262 22.116518230576194 58-59 20.319637848742804 30.578015056832157 27.494661221276385 21.60768587314865 60-61 19.303596909905032 28.243812429267333 31.68213354327609 20.77045711755154 62-63 20.113270678541554 28.432219652610343 30.823844904787677 20.630664764060423 64-65 20.63954203566603 29.98625201619054 28.624493061549046 20.749712886594388 66-67 20.571027899424298 31.721891453033507 27.459085764896912 20.24799488264528 68-69 21.506003317435034 28.41076557366482 30.55799532842951 19.52523578047063 70-71 22.528416080303103 28.450622447473307 29.33331693155538 19.68764454066821 72-73 20.962948383604783 30.578654726172317 28.456773114205582 20.001623776017322 74-75 19.842198494316783 31.162722039069035 30.416916793780445 18.578162672833734 76-77 20.444865423411898 29.117354721251782 30.873443881316735 19.564335974019585 78-79 19.94621843777601 28.950619865718313 30.085150039733406 21.01801165677227 80-81 20.647591398907643 29.96088175958274 29.743787826600403 19.647739014909217 82-83 19.89785876383419 29.859064973202564 31.128917021439918 19.11415924152333 84-85 19.68430826369545 29.569418673138152 31.080859656969768 19.665413406196627 86-87 18.36149949441146 32.11035853731145 28.353651185734286 21.174490782542804 88-89 28.520372260216593 18.90594489208414 35.969780512695536 16.60390233500373 90-91 30.65074025124488 15.775361448195763 17.67451640033642 35.89938190022294 92-93 22.387670555853045 17.89449278997486 36.89823291058612 22.81960374358598 94-95 15.866209491518784 7.185639405624822 28.296749527436297 48.6514015754201 96-97 27.264228943934928 7.333240935007336 15.3740937396948 50.02843638136294 98-99 57.25111145831854 13.899265656293055 18.669658963396447 10.17996392199196 100-101 30.542024413268333 7.288938018741087 13.012815535567606 49.15622203242298 102-103 23.126978247693447 2.1528175297999863 37.61459104990235 37.105613172604215 104-105 40.754829391233116 4.575893513319654 42.496095135626675 12.173181959820562 106-107 5.8924942219934255 1.9696165334105522 6.741365164653078 85.39652407994295 108-109 18.266553795081748 11.343594284101835 23.147179702940463 47.242672217875956 110-111 40.2951724382978 2.8703242358440058 26.15343120193616 30.681072123922032 112-113 27.190116607592334 2.7868700878409616 39.52021369497098 30.502799609595726 114-115 28.908809914776317 4.070229665569483 24.254199885575872 42.76676053407833 116-117 20.008033588609898 4.35910141086235 20.642511170792922 54.99035382973483 118-119 20.6734070763737 4.374927366274709 16.007944808933683 58.9437207484179 120-121 25.603332755079204 4.244888107826796 30.329177446077804 39.822601691016196 122-123 34.38519953111462 1.7230518322205801 34.10896488456246 29.782783752102336 124-125 38.916995821563724 2.090104859932235 20.24694252950571 38.74595678899833 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 23.0 1 60.5 2 147.5 3 237.0 4 284.5 5 295.5 6 264.5 7 190.5 8 145.0 9 125.0 10 111.5 11 106.0 12 106.0 13 117.5 14 124.5 15 157.0 16 209.0 17 264.5 18 383.0 19 567.0 20 817.0 21 1220.0 22 1767.0 23 2490.5 24 3483.0 25 4743.5 26 6358.0 27 8617.0 28 11543.5 29 15300.5 30 20288.0 31 26936.0 32 35131.5 33 44622.5 34 53492.0 35 59438.0 36 61887.5 37 60343.5 38 56943.0 39 53092.5 40 49026.5 41 45030.5 42 40835.0 43 37452.5 44 34553.5 45 32129.0 46 29952.5 47 27542.5 48 25503.0 49 23997.0 50 22071.5 51 19662.0 52 17315.5 53 14888.5 54 12604.0 55 10504.5 56 8667.5 57 7086.5 58 5729.5 59 4562.5 60 3612.5 61 2823.0 62 2120.0 63 1579.0 64 1201.0 65 870.5 66 621.0 67 448.0 68 326.5 69 240.5 70 179.0 71 133.0 72 103.5 73 86.5 74 70.0 75 51.0 76 43.0 77 27.5 78 14.0 79 12.5 80 5.5 81 3.5 82 5.5 83 4.5 84 2.5 85 2.0 86 4.5 87 4.5 88 2.0 89 1.0 90 1.5 91 2.0 92 1.5 93 2.0 94 2.0 95 1.0 96 2.5 97 2.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 7.872853417310436E-4 3 0.04044678443143237 4 0.045170496481818624 5 0.04635142449441519 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.364906755892339E-4 12-13 0.0012301333464547557 14-15 4.9205333858190226E-5 16-17 3.936426708655218E-4 18-19 0.0068395414062884415 20-21 0.0013777493480293264 22-23 5.412586724400925E-4 24-25 0.0 26-27 0.0 28-29 0.0010333120110219947 30-31 0.0 32-33 0.001082517344880185 34-35 1.4761600157457068E-4 36-37 4.9205333858190226E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 1.4761600157457068E-4 46-47 0.0 48-49 1.4761600157457068E-4 50-51 4.9205333858190226E-5 52-53 0.0 54-55 0.0018205973527530386 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 9.841066771638045E-5 66-67 0.0 68-69 3.444373370073316E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.460266692909511E-4 80-81 0.0 82-83 7.872853417310436E-4 84-85 4.9205333858190226E-5 86-87 7.380800078728534E-4 88-89 19.238055405205927 90-91 26.28352113369089 92-93 40.36347980121045 94-95 44.73606258918467 96-97 48.521625744230676 98-99 93.07159376076368 100-101 51.83722875559711 102-103 53.238399842542925 104-105 74.89243714018599 106-107 81.64808345224623 108-109 12.637455100132854 110-111 39.067706539388865 112-113 52.10598828913054 114-115 86.75520346405551 116-117 66.43527038330956 118-119 72.05540520592433 120-121 80.4286276632387 122-123 75.86355360921124 124-125 50.05791467795109 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1016150.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.71696361685194 #Duplication Level Percentage of deduplicated Percentage of total 1 86.86651635771018 63.166693095062854 2 9.11470319877795 13.255870817678803 3 1.9901277432876345 4.341481401046037 4 0.7348578473690882 2.137465254027845 5 0.3389072685105764 1.2322153756885128 6 0.20575577575117093 0.8977161155553026 7 0.13981384023745838 0.7116786553575728 8 0.0953264063081306 0.5545477455386864 9 0.06968032826737386 0.45602477058860325 >10 0.38138279985969203 5.021703059018107 >50 0.03411285986380428 1.7155456977706252 >100 0.024892328911483914 3.6018904629010424 >500 0.002840970622516049 1.3973134771547675 >1k 0.0010822745228632569 1.5098540726113328 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 3079 0.3030064458987354 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 2621 0.25793436008463316 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 2029 0.19967524479653592 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1938 0.19071987403434532 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 1769 0.17408847119027704 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 1698 0.16710131378241402 No Hit GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA 1136 0.11179451852580821 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1078 0.10608669979825813 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 9.841066771638045E-5 0.0 0.0 0.0 0.0 8 9.841066771638045E-5 0.0 0.0 0.0 0.0 9 9.841066771638045E-5 0.0 0.0 0.0 0.0 10-11 9.841066771638045E-5 0.0 0.0 0.0 0.0 12-13 9.841066771638045E-5 0.0 0.0 0.0 0.0 14-15 9.841066771638045E-5 0.0 0.0 4.9205333858190226E-5 0.0 16-17 1.4761600157457068E-4 0.0 0.0 9.841066771638045E-5 0.0 18-19 1.968213354327609E-4 0.0 0.0 9.841066771638045E-5 0.0 20-21 1.968213354327609E-4 0.0 0.0 1.4761600157457068E-4 0.0 22-23 1.968213354327609E-4 0.0 0.0 5.412586724400925E-4 0.0 24-25 1.968213354327609E-4 0.0 0.0 8.364906755892339E-4 0.0 26-27 1.968213354327609E-4 0.0 0.0 0.001082517344880185 0.0 28-29 1.968213354327609E-4 0.0 0.0 0.00216503468976037 0.0 30-31 4.4284800472371206E-4 0.0 0.0 0.0037396053732224573 0.0 32-33 4.920533385819023E-4 0.0 0.0 0.007183978743295773 0.0 34-35 4.920533385819023E-4 0.0 0.0 0.011661664124391083 0.0 36-37 4.920533385819023E-4 0.0 0.0 0.020371008217290754 0.0 38-39 4.920533385819023E-4 0.0 0.0 0.03365644835900211 0.0 40-41 4.920533385819023E-4 0.0 0.0 0.053043349899129066 0.0 42-43 4.920533385819023E-4 0.0 0.0 0.07060965408650298 0.0 44-45 4.920533385819023E-4 0.0 0.0 0.09029178762977907 0.0 46-47 4.920533385819023E-4 0.0 0.0 0.11701028391477636 0.0 48-49 4.920533385819023E-4 0.0 0.0 0.14407321753678098 0.0 50-51 4.920533385819023E-4 0.0 0.0 0.17590906854303007 0.0 52-53 4.920533385819023E-4 0.0 0.0 0.20990995423903952 0.0 54-55 4.920533385819023E-4 0.0 0.0 0.24720759730354772 0.0 56-57 4.920533385819023E-4 0.0 0.0 0.2894257737538749 0.0 58-59 5.904640062982827E-4 0.0 0.0 0.339615214289229 0.0 60-61 5.904640062982827E-4 0.0 0.0 0.4003838016040939 0.0 62-63 5.904640062982827E-4 0.0 0.0 0.4641047089504502 0.0 64-65 5.904640062982827E-4 0.0 0.0 0.5241352162574423 0.0 66-67 5.904640062982827E-4 0.0 0.0 0.5987797077203169 0.0 68-69 6.396693401564729E-4 0.0 0.0 0.6926634847217439 0.0 70-71 6.888746740146632E-4 0.0 0.0 0.7945185258081976 0.0 72-73 7.872853417310436E-4 0.0 0.0 0.8919942921812725 0.0 74-75 7.872853417310436E-4 0.0 0.0 0.9900605225606456 0.0 76-77 7.872853417310436E-4 0.0 0.0 1.0963932490281947 0.0 78-79 7.872853417310436E-4 0.0 0.0 1.127540225360429 0.0 80-81 7.872853417310436E-4 0.0 0.0 1.1281798947005854 0.0 82-83 7.872853417310436E-4 0.0 0.0 1.1281798947005854 0.0 84-85 7.872853417310436E-4 0.0 0.0 1.1281798947005854 0.0 86-87 7.872853417310436E-4 0.0 0.0 1.1281798947005854 0.0 88-89 7.872853417310436E-4 0.0 0.0 1.1281798947005854 0.0 90-91 7.872853417310436E-4 0.0 0.0 1.1281798947005854 0.0 92-93 7.872853417310436E-4 0.0 0.0 1.1281798947005854 0.0 94-95 7.872853417310436E-4 0.0 0.0 1.1281798947005854 0.0 96-97 7.872853417310436E-4 0.0 0.0 1.1281798947005854 0.0 98-99 7.872853417310436E-4 0.0 0.0 1.1281798947005854 0.0 100-101 7.872853417310436E-4 0.0 0.0 1.1281798947005854 0.0 102-103 7.872853417310436E-4 0.0 0.0 1.1281798947005854 0.0 104-105 7.872853417310436E-4 0.0 0.0 1.1281798947005854 0.0 106-107 7.872853417310436E-4 0.0 0.0 1.1281798947005854 0.0 108-109 7.872853417310436E-4 0.0 0.0 1.1281798947005854 0.0 110-111 7.872853417310436E-4 0.0 0.0 1.1281798947005854 0.0 112-113 7.872853417310436E-4 0.0 0.0 1.1281798947005854 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTCG 5 2.2809466E-4 5982.2686 110-111 CGTCGCT 45 0.0 3988.1787 110-111 CGACGCG 10 0.0012978456 2508.415 108-109 CCGCGCT 30 1.371518E-9 2508.4148 108-109 CGTGTCG 25 1.5769638E-6 2392.9072 110-111 CGTAGCG 15 0.002052398 1994.0894 110-111 CCGCTCG 40 4.3328328E-9 1881.3113 108-109 CTCCGCC 115 0.0 1820.6903 110-111 CCGCGCG 45 6.9394446E-9 1672.2767 108-109 CCGCTAT 15 0.002919767 1672.2766 108-109 CCGAACG 15 0.002919767 1672.2766 108-109 CCGCTCC 255 0.0 1573.9075 108-109 CGGCGTG 40 6.4568376E-6 1495.5671 110-111 CCGCACG 35 7.3461615E-6 1433.3799 108-109 CCGCGCC 200 0.0 1379.6282 108-109 CGTTGGT 45 9.192287E-6 1329.3928 110-111 TCGTTCG 20 0.0051900106 1254.2075 108-109 CGACGCC 40 1.0964071E-5 1254.2075 108-109 CAGTTCG 60 2.1920641E-8 1254.2074 108-109 CTTATCG 25 0.0056998427 1196.4536 110-111 >>END_MODULE