FastQCFastQC Report
Fri 27 May 2016
SRR939972_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939972_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016150
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA31710.3120602273286424No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC25620.25212813068936674No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23390.23018255178861388No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21550.21207498892879986No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA18530.18235496727845296No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA18070.17782807656349947No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA17150.1687742951335925No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17050.16779018845642868No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11630.11445160655415047No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10800.1062835211336909No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGA558.531405E-443.269179
TACCTGG10100.040.701252
GTACCTG10650.038.6031071
TAGGACC21750.035.6093564
GCGTATA851.6973591E-435.0454032
CACCGTC953.2876356E-431.3132137
GTACACG1152.7374424E-531.0868131
TAGAACG2550.030.3726834
ACCTGGG14400.029.7885953
GTACATG48800.028.082181
GTATCAA68200.028.0442281
TACATGG48700.027.647732
ACATGGG46600.027.1037833
ACGGACC900.00946842126.478754
TGATCGC450.00953447426.44226852-53
AGAACGT3200.026.0650225
AGTACTT32000.025.56430212-13
ATCAACG75350.025.1433933
TCAACGC75750.025.010624
CAACGCA75800.024.9941235
Produced by FastQC (version 0.11.5)