FastQCFastQC Report
Fri 27 May 2016
SRR939971_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939971_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1544956
Sequences flagged as poor quality0
Sequence length125
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT86030.5568443373144607No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT50790.32874722645822924No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT35440.22939164610513177No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC29610.19165594359968827No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT28390.1837592785813965No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT22950.14854791981130855No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC21050.13624983494675577No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC19450.12589355295555343No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG16840.10899986795740461No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC15790.10220355790067807No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA15770.10207410437578805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC7300.01552.7362108-109
CCGCGCC6250.01143.3539108-109
CCGCGCT1001.4733814E-10985.64984108-109
CTCCGCC3850.0937.3565110-111
CCGCGCG3150.0782.2618108-109
CCGTTCG1001.8251376E-7739.23737108-109
TTGTTCG2200.0672.03406108-109
CCGCGCA1153.1906347E-7642.8151108-109
CTCCGCT2102.2027962E-9572.829110-111
CGACGCC901.319457E-4547.5833108-109
GAGCGCG951.551575E-4518.7631108-109
CCGCACC5800.0509.8189108-109
TGTCGTT1201.7179165E-4501.2254110-111
CGTCGCC3250.0454.91534108-109
CCGCTCA1152.750633E-4428.54343108-109
CCGCTCG2905.820766E-11424.8491108-109
CTTCGCT2901.1039447E-8414.8072110-111
GTTCGCT1453.0289596E-4414.8072110-111
CGGCGCC3800.0389.07236108-109
TTTCGCG855.3376425E-7353.80615110-111
Produced by FastQC (version 0.11.5)