FastQCFastQC Report
Fri 27 May 2016
SRR939971_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939971_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1544956
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT93430.6047421415237716No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT47830.30958810477450494No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35220.22796765733134144No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT34760.2249902262588708No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT29910.1935977464730387No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC28790.1863483490791971No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24270.15709185245405047No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT23560.15249625232045444No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC23400.1514606241213342No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC21430.13870945191966633No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG19480.12608773324288847No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA17120.110812217305865No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA16410.10621661717226899No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC15600.1009737494142228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG71800.027.058241
TACATGG70750.026.5229362
GAGTACT53900.025.82866712-13
ACATGGG70750.025.5967373
GTACTTT55600.024.8804514-15
AGAGTAC94550.024.35142310-11
AGTACTT56800.024.03860712-13
CATGGGG37150.023.251274
GTACCTG16600.022.9762041
TACCTGG17000.022.4268632
ATTCGCG953.3104327E-521.9189380-81
GTAGGAC19350.021.8582253
GTAAGGT9650.021.6061924
TAAGGTG10050.021.3389935
GGACCGA4201.8189894E-1221.2479446
TACTTTT64150.021.05422614-15
GTGTAGC12150.020.6005761
ACTTTTT69800.020.03353716-17
TATGCGA1057.147329E-519.83462316-17
TAGGACG19350.018.7796024
Produced by FastQC (version 0.11.5)