FastQCFastQC Report
Fri 27 May 2016
SRR939970_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939970_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1318493
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28470.21592833636583583No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15910.12066806573868803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC4900.02090.4714108-109
CGTCGGT200.00354476371517.8259110-111
CTCCGCC2550.01428.542110-111
CGTCGCC1900.01051.9446108-109
CGTCGCG552.8892646E-5908.49756108-109
CGGCGCG951.4040597E-7788.95844108-109
CGTATCT804.9479022E-5758.91296110-111
CCGCGCC5850.0726.02155108-109
CGGCGCC2500.0699.5431108-109
TTGTTCG1903.6379788E-12657.46533108-109
TGTCGCG500.005007817607.1304110-111
CATCGCG1001.7342692E-4499.67365108-109
CCGTTCG1052.007334E-4475.87973108-109
CCTCTCG2653.092282E-11471.3903108-109
CTGCGCG550.0020700172454.24878108-109
GGTCTCG1352.3744725E-4449.72623110-111
CGTGGTT1503.2559672E-4404.75357110-111
CGCTGTC1503.2559672E-4404.75357110-111
TTTTTCG8100.0402.0623592-93
CGTGTCT2352.3976463E-6387.53003110-111
Produced by FastQC (version 0.11.5)