##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939970_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1318493 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.85261886107852 34.0 31.0 34.0 30.0 34.0 2 32.00289648864272 34.0 31.0 34.0 30.0 34.0 3 32.11630930160418 34.0 31.0 34.0 30.0 34.0 4 35.33418759143962 37.0 35.0 37.0 33.0 37.0 5 35.3740285310578 37.0 35.0 37.0 33.0 37.0 6 35.443130149344746 37.0 37.0 37.0 33.0 37.0 7 35.39403546321444 37.0 37.0 37.0 33.0 37.0 8 35.338929368604916 37.0 37.0 37.0 33.0 37.0 9 36.99660976584631 39.0 39.0 39.0 34.0 39.0 10-11 36.99312131349958 39.0 39.0 39.0 34.0 39.0 12-13 36.93648999274171 39.0 38.0 39.0 34.0 39.0 14-15 38.44420220661012 41.0 39.0 41.0 34.0 41.0 16-17 38.45513665980783 41.0 39.0 41.0 34.0 41.0 18-19 38.49274778098936 41.0 39.0 41.0 34.0 41.0 20-21 38.55200975659332 41.0 39.0 41.0 34.0 41.0 22-23 38.54976628620706 41.0 39.0 41.0 34.0 41.0 24-25 38.57775543745777 41.0 39.0 41.0 34.0 41.0 26-27 38.5488610861036 41.0 39.0 41.0 34.0 41.0 28-29 38.4981308205656 41.0 39.0 41.0 34.0 41.0 30-31 38.42975161794564 41.0 39.0 41.0 34.0 41.0 32-33 38.325612270979065 41.0 39.0 41.0 33.0 41.0 34-35 38.24539227739548 40.0 38.0 41.0 33.0 41.0 36-37 38.40967680526177 41.0 39.0 41.0 33.5 41.0 38-39 38.382941358050445 41.0 39.0 41.0 33.0 41.0 40-41 38.33048980919884 41.0 39.0 41.0 33.0 41.0 42-43 38.24339302521894 41.0 39.0 41.0 33.0 41.0 44-45 38.13774210405364 41.0 38.0 41.0 33.0 41.0 46-47 38.04792175612612 41.0 38.0 41.0 33.0 41.0 48-49 37.94740548489829 40.5 38.0 41.0 33.0 41.0 50-51 37.82898923240397 40.0 38.0 41.0 33.0 41.0 52-53 37.71598104806017 40.0 38.0 41.0 32.5 41.0 54-55 37.553169034647894 40.0 37.5 41.0 32.0 41.0 56-57 37.36373571949187 40.0 37.0 41.0 32.0 41.0 58-59 37.18613902387044 40.0 37.0 41.0 31.0 41.0 60-61 36.95690458728261 40.0 36.0 41.0 31.0 41.0 62-63 36.70644705736018 39.5 36.0 41.0 31.0 41.0 64-65 36.41439355385277 39.0 35.0 41.0 31.0 41.0 66-67 36.098846941166926 39.0 35.0 41.0 30.5 41.0 68-69 35.7272302545406 38.0 35.0 40.5 30.0 41.0 70-71 35.3301413052629 37.0 35.0 40.0 30.0 41.0 72-73 34.93686617979769 37.0 35.0 39.0 30.0 41.0 74-75 34.525106693778426 36.0 35.0 39.0 29.0 41.0 76-77 34.08924506993969 36.0 34.0 38.5 29.0 40.0 78-79 33.646927211596875 35.0 34.0 37.0 29.0 39.0 80-81 33.22173458638005 35.0 34.0 37.0 28.0 39.0 82-83 33.01750407472774 35.0 34.0 36.5 29.0 39.0 84-85 32.626638139148255 35.0 34.0 36.0 29.0 37.0 86-87 32.01570277582058 35.0 34.0 36.0 26.0 37.0 88-89 16.560255154938254 18.0 17.0 18.5 2.0 19.0 90-91 2.8101882224630694 2.0 2.0 2.0 2.0 2.0 92-93 2.3054180037360834 2.0 2.0 2.0 2.0 2.0 94-95 2.1039489781136496 2.0 2.0 2.0 2.0 2.0 96-97 2.0000109974038542 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08522344921717817 1101 2 0.19047650943890915 1101 3 0.2260024001024732 1101 4 0.2206780234307999 1101 5 0.197632363194451 1101 6 0.1108764084972762 1101 7 0.18292347132610587 1101 8 0.15154736463846774 1101 9 0.1976898279201933 1101 10-11 0.1796820726139714 1101 12-13 0.1950808672141875 1101 14-15 0.1472034047036459 1101 16-17 0.2895076036730586 1101 18-19 0.24318636283190642 1101 20-21 0.2502085719261018 1101 22-23 0.1886444898167241 1101 24-25 0.21161605287572627 1101 26-27 0.19880462995183734 1101 28-29 0.2669204286199971 1101 30-31 0.2126662074595984 1101 32-33 0.20282350326523613 1101 34-35 0.24886991861605878 1101 36-37 0.2787387842399198 1101 38-39 0.2489928169969602 1101 40-41 0.22384577260058336 1101 42-43 0.24767005537231768 1101 44-45 0.19995010547506809 1101 46-47 0.22008480237806793 1101 48-49 0.2849571979115453 1101 50-51 0.26817635135871143 1101 52-53 0.26094722316976515 1101 54-55 0.34907619297973014 1101 56-57 0.2899986633460685 1101 58-59 0.31190306271363966 1101 60-61 0.2926368319165533 1101 62-63 0.19946111533779742 1101 64-65 0.16036065613293005 1101 66-67 0.20094880566304596 1101 68-69 0.22919143498882733 1101 70-71 0.16177931792103095 1101 72-73 0.2767481180079443 1101 74-75 0.3234484382628082 1101 76-77 0.33961945142785055 1101 78-79 0.3478814428451855 1101 80-81 0.2806289121562031 1101 82-83 0.2939111244237935 1101 84-85 0.3755004252217091 1101 86-87 0.3326748744446917 1101 88-89 0.9418418082050124 1101 90-91 -0.010817357633357894 1101 92-93 -0.18008673552039056 1101 94-95 -0.0020907385566526493 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.08012088409288509 1102 2 0.14390957313813146 1102 3 0.2690942232051654 1102 4 0.2616851980610093 1102 5 0.16720572331642813 1102 6 0.18234377871532104 1102 7 0.2115443945695219 1102 8 0.11088833049763025 1102 9 0.2381328471790738 1102 10-11 0.14843856092504382 1102 12-13 0.16493001332623436 1102 14-15 0.2154211141489526 1102 16-17 0.2273404081544257 1102 18-19 0.09053724440332189 1102 20-21 0.13184814585247295 1102 22-23 0.17675947684534776 1102 24-25 0.20718669917966537 1102 26-27 0.17163079669435177 1102 28-29 0.15295370330075286 1102 30-31 0.14876250395784751 1102 32-33 0.23160171889843184 1102 34-35 0.10232374491073415 1102 36-37 0.19632700954134208 1102 38-39 0.18898052721824143 1102 40-41 0.18234365125055518 1102 42-43 0.2229353027722638 1102 44-45 0.22533919441657702 1102 46-47 0.24682765448757493 1102 48-49 0.21710440681051324 1102 50-51 0.16649940476052194 1102 52-53 0.14352530835513022 1102 54-55 0.15921064651450934 1102 56-57 0.14845753348337354 1102 58-59 0.15751009599041765 1102 60-61 0.11709957354387512 1102 62-63 0.19518485956778875 1102 64-65 0.18725346032068302 1102 66-67 0.1360594031853708 1102 68-69 0.1617654305720606 1102 70-71 0.2111649338465753 1102 72-73 0.11099549489519234 1102 74-75 0.19308660284936963 1102 76-77 0.12640122816074495 1102 78-79 0.1597461126674915 1102 80-81 0.1179602684989689 1102 82-83 0.18904428816097862 1102 84-85 0.13668548705859251 1102 86-87 0.2609810031853783 1102 88-89 0.6953971405218873 1102 90-91 -0.09863369884459328 1102 92-93 -0.13821969582608284 1102 94-95 0.021527238075989885 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.09441038388276013 1103 2 0.043752151990695154 1103 3 0.08339930568015319 1103 4 -0.059641799592967004 1103 5 -0.08373115823034993 1103 6 -0.08840137232014911 1103 7 -0.06548828233267301 1103 8 0.035875770299696796 1103 9 -0.02659797139632758 1103 10-11 -0.025584182862935734 1103 12-13 -6.278549361837804E-5 1103 14-15 -0.008510540899230534 1103 16-17 -0.021702468356622262 1103 18-19 0.10187258639749075 1103 20-21 0.11556896432593788 1103 22-23 0.18769129062530965 1103 24-25 0.1439785406575922 1103 26-27 0.13282597416085196 1103 28-29 0.2304159753786621 1103 30-31 0.11577556510744813 1103 32-33 0.19510607965352733 1103 34-35 0.17052525562949938 1103 36-37 0.16549780347691723 1103 38-39 0.186007437351293 1103 40-41 0.08518087254157791 1103 42-43 0.04786332737715071 1103 44-45 0.03737921812440703 1103 46-47 0.02705812137743635 1103 48-49 0.009666439625767964 1103 50-51 0.02796690188078088 1103 52-53 0.06418286566240283 1103 54-55 0.0016191172576256463 1103 56-57 0.0521584595158302 1103 58-59 0.07758005447259109 1103 60-61 0.04203298629163754 1103 62-63 -0.023966202114799273 1103 64-65 -0.05560062440131475 1103 66-67 -0.1463892310660313 1103 68-69 -0.1257841731081868 1103 70-71 -0.07617962915318799 1103 72-73 -0.02918505157899176 1103 74-75 -0.06298969325384718 1103 76-77 -0.00890232087790821 1103 78-79 -0.04374190890838037 1103 80-81 -0.13577466108383618 1103 82-83 -0.13989033036578746 1103 84-85 -0.09551128791945729 1103 86-87 -0.1105920287226283 1103 88-89 0.7319099635738198 1103 90-91 -0.5728402219924917 1103 92-93 -0.32018601847681616 1103 94-95 -0.10992306067468904 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.057931199562933244 1104 2 0.09516972967617221 1104 3 0.11750590529954508 1104 4 0.1570551957813251 1104 5 0.04326880009661238 1104 6 0.1256591091422976 1104 7 0.20616294215995623 1104 8 0.16404118446018856 1104 9 0.10071175647876629 1104 10-11 0.1568978630051845 1104 12-13 0.2190463839628265 1104 14-15 0.21033053204246954 1104 16-17 0.2008857048365016 1104 18-19 0.15491541238478845 1104 20-21 0.11543838882135304 1104 22-23 -0.1091533661972619 1104 24-25 0.1315259437330596 1104 26-27 0.15068911991922818 1104 28-29 0.16222777995992033 1104 30-31 0.1718230168014614 1104 32-33 0.178595918378889 1104 34-35 0.222119249156286 1104 36-37 0.21640071920015203 1104 38-39 0.10932836138135826 1104 40-41 0.25085479494639173 1104 42-43 0.2763933805797265 1104 44-45 0.23741741840500197 1104 46-47 0.1991712219900279 1104 48-49 0.18893966192361944 1104 50-51 0.10432717914535772 1104 52-53 0.1513371531229808 1104 54-55 0.1793136261409245 1104 56-57 0.12106759841204706 1104 58-59 0.18190154803411218 1104 60-61 0.23598067274374301 1104 62-63 0.11685392318212706 1104 64-65 0.0612790377693031 1104 66-67 0.16894505611673338 1104 68-69 0.08530988292815067 1104 70-71 0.06325070048050208 1104 72-73 0.05989730822597039 1104 74-75 0.06951527278263114 1104 76-77 0.09629847368792355 1104 78-79 0.1200449196348714 1104 80-81 0.06445870790309272 1104 82-83 0.1601860701954081 1104 84-85 0.189757989331234 1104 86-87 0.2171003965090037 1104 88-89 0.7458954335761803 1104 90-91 -0.601562665699169 1104 92-93 -0.32018601847681616 1104 94-95 -0.10992306067468904 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.20268160704739557 1105 2 -0.1880697386725565 1105 3 -0.13258089220386893 1105 4 -0.07939180473547935 1105 5 -0.18815852190224547 1105 6 -0.15318747792686338 1105 7 -0.0798434450761718 1105 8 -0.17850681919723144 1105 9 -0.02538845157021541 1105 10-11 -0.09702127617561018 1105 12-13 -0.03475282889645115 1105 14-15 -0.14935103375798775 1105 16-17 -0.07557958278789556 1105 18-19 -0.11775331268577105 1105 20-21 -0.07746806864787459 1105 22-23 -0.24889668411465493 1105 24-25 -0.129741829877851 1105 26-27 -0.10714783328963051 1105 28-29 -0.11649954336409962 1105 30-31 -0.17711884874854888 1105 32-33 -0.20979508118381318 1105 34-35 -0.17974524072617015 1105 36-37 -0.18441888775955562 1105 38-39 -0.21349408140517312 1105 40-41 -0.08823986071266887 1105 42-43 -0.05982370593950037 1105 44-45 -0.14785284649302355 1105 46-47 -0.02485180916431773 1105 48-49 -0.08335139462064944 1105 50-51 -0.15644167446502166 1105 52-53 -0.09803472448591322 1105 54-55 -0.17112897486884293 1105 56-57 -0.10804600693096944 1105 58-59 -0.1897176560791607 1105 60-61 -0.16480381766877628 1105 62-63 -0.026952975562956283 1105 64-65 -0.03480977396625207 1105 66-67 -0.018936422223646332 1105 68-69 -0.09639572114271999 1105 70-71 -0.22765278864662264 1105 72-73 -0.12050037010645553 1105 74-75 -0.12827702277942876 1105 76-77 -0.10179330638972317 1105 78-79 -0.06680095185181756 1105 80-81 -0.05583733121540746 1105 82-83 0.006525335795771525 1105 84-85 -0.0934158839576682 1105 86-87 0.13365486691171924 1105 88-89 1.0451838265634734 1105 90-91 -0.8025989351292209 1105 92-93 -0.32018601847681616 1105 94-95 -0.10992306067468904 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.06999551030706641 1106 2 0.029983335247102616 1106 3 0.07046973576355242 1106 4 0.11844719321562991 1106 5 0.04269940395620608 1106 6 0.16614722306358942 1106 7 0.03577548464779312 1106 8 0.19001916044881284 1106 9 0.24066990612532635 1106 10-11 0.046590636195716684 1106 12-13 0.1508824893563201 1106 14-15 0.17768160445778136 1106 16-17 0.13693694523156097 1106 18-19 0.08354531277989707 1106 20-21 0.12138629144040891 1106 22-23 0.10781009097841832 1106 24-25 0.04240532691353138 1106 26-27 -0.05190694267754736 1106 28-29 0.09124069521032396 1106 30-31 0.13128653312492844 1106 32-33 0.18011838271276304 1106 34-35 0.034601274797516623 1106 36-37 0.1744992357733608 1106 38-39 0.22202581644874897 1106 40-41 0.17821893245319842 1106 42-43 -0.12467167411227109 1106 44-45 0.05573628024363586 1106 46-47 -0.0665733962175068 1106 48-49 0.0014918762857547563 1106 50-51 -0.04824364705738304 1106 52-53 -0.10604291485755368 1106 54-55 -0.05265444456303925 1106 56-57 -0.1841297843635914 1106 58-59 -0.08536658602371716 1106 60-61 0.09167055952170955 1106 62-63 0.04631425866843841 1106 64-65 -0.03923843974796881 1106 66-67 0.08911471829079431 1106 68-69 0.035403993647570076 1106 70-71 0.04670338092729054 1106 72-73 0.061342748982227135 1106 74-75 -0.03411824220015802 1106 76-77 -0.05828390342266232 1106 78-79 0.01984946902064877 1106 80-81 0.23334091966273007 1106 82-83 0.15811247981888954 1106 84-85 0.1501070139183156 1106 86-87 0.25696693278763405 1106 88-89 1.0275790690890467 1106 90-91 -0.27900684989330005 1106 92-93 -0.3057263533322194 1106 94-95 -0.10992306067468904 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.2343476629507535 1107 2 -0.27426653044142313 1107 3 -0.15722474483924032 1107 4 -0.2442335446831052 1107 5 -0.21558592300008428 1107 6 -0.22097275653069204 1107 7 -0.3251481223313917 1107 8 -0.2788093066425361 1107 9 -0.2359710431559776 1107 10-11 -0.31410702862110895 1107 12-13 -0.2860450721172967 1107 14-15 -0.27529013022837745 1107 16-17 -0.3433727779057065 1107 18-19 -0.37048482922170933 1107 20-21 -0.40531338507221193 1107 22-23 -0.28698596962372847 1107 24-25 -0.28805129662401896 1107 26-27 -0.37367834442829206 1107 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2216 2 -0.040549657071128564 2216 3 -0.054346594532233894 2216 4 0.032211862614410336 2216 5 0.11957764919503688 2216 6 0.08813417377346866 2216 7 0.019399363101541667 2216 8 -0.012253338332740782 2216 9 -0.08746318073511361 2216 10-11 -0.11290098264190362 2216 12-13 -0.07457070428070267 2216 14-15 -0.07616464585334626 2216 16-17 -0.12040904587492918 2216 18-19 -0.06684885042911048 2216 20-21 -0.1424314197922314 2216 22-23 -0.12557684744608366 2216 24-25 -0.21911960827468135 2216 26-27 -0.20486275758651828 2216 28-29 -0.23702225965448775 2216 30-31 -0.18679241089302678 2216 32-33 -0.26990199019758165 2216 34-35 -0.26944841767462435 2216 36-37 -0.29101889611683873 2216 38-39 -0.27023375292085916 2216 40-41 -0.31315079665404966 2216 42-43 -0.3212457093959884 2216 44-45 -0.3716054407348537 2216 46-47 -0.4194046781252325 2216 48-49 -0.3979285425778798 2216 50-51 -0.4735868673630321 2216 52-53 -0.5396651673043777 2216 54-55 -0.5945227414026135 2216 56-57 -0.5745103486283796 2216 58-59 -0.4908274612150336 2216 60-61 -0.5438164146749429 2216 62-63 -0.5648405314523259 2216 64-65 -0.5901848501729461 2216 66-67 -0.6116647235403434 2216 68-69 -0.5668668472445617 2216 70-71 -0.5942584542457681 2216 72-73 -0.6382442877678756 2216 74-75 -0.662484049973834 2216 76-77 -0.5976291263737181 2216 78-79 -0.4498024400921494 2216 80-81 -0.4340530928483801 2216 82-83 -0.4203648847830834 2216 84-85 -0.5100233174448974 2216 86-87 -0.5030027745970074 2216 88-89 -1.1078837806910808 2216 90-91 -0.8025989351292209 2216 92-93 -0.32018601847681616 2216 94-95 -0.10992306067468904 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12803.0 3 2512.0 4 1793.0 5 1563.0 6 1764.0 7 1807.0 8 1930.0 9 2196.0 10 2737.0 11 3347.0 12 4027.0 13 4583.0 14 4807.0 15 5527.0 16 6339.0 17 7599.0 18 9723.0 19 12097.0 20 12107.0 21 17522.0 22 20219.0 23 24898.0 24 38663.0 25 66311.0 26 133908.0 27 391550.0 28 507079.0 29 18432.0 30 650.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.97451181007408 20.638486514528328 15.162689525086595 22.224312150311 2 13.869165087835594 21.444747991256637 41.479014566707576 23.207072354200196 3 18.3669737578891 27.520603843218066 29.29554659556678 24.816875803326056 4 11.844504456789215 18.31642861966633 39.7002148151914 30.138852108353053 5 13.206877136964534 39.41833589554085 34.111303508529645 13.26348345896497 6 29.06720020508262 37.966451092269736 19.740567450870046 13.225781251777596 7 27.675080565463755 33.07222715630648 22.406338145139944 16.84635413308982 8 25.554250193213008 35.668069530896254 21.152634105755585 17.625046170135146 9 26.331121970310043 18.143289346246057 21.54922324198915 33.97636544145475 10-11 24.00014713896251 28.0176475741222 28.347496722555455 19.634708564359833 12-13 25.292451052110838 25.469436698715214 28.31322856784216 20.92488368133179 14-15 22.054657117882734 27.53110085950432 26.209098648646084 24.20514337396686 16-17 21.39237731762036 30.17639170988543 27.438402834766546 20.992828137727667 18-19 21.600491205554658 29.100705482221105 28.684136472619237 20.614666839605004 20-21 23.086549626877844 28.117915552053173 27.68457203013495 21.11096279093403 22-23 22.339381055005912 28.591857855092073 27.55403832654967 21.514722763352346 24-25 22.26022436220746 28.512400141676896 28.050129958975887 21.17724553713975 26-27 21.576565063295746 28.78528744559129 28.16078659499899 21.47736089611397 28-29 21.39566280046493 28.873568187549655 27.800777791050663 21.929991220934753 30-31 21.756391577353842 28.370306099463555 28.005078525255726 21.868223797926873 32-33 21.474536311065318 28.802469215393078 27.862027015291524 21.860967458250077 34-35 22.12138725254856 28.72344217745893 27.89131666427757 21.263853905714942 36-37 21.892868438237347 28.10493207765544 28.098599080312265 21.903600403794947 38-39 21.8322357418659 28.728063023466945 27.72839901311573 21.711302221551424 40-41 22.265609297887814 28.21565984802346 28.124533084362223 21.394197769726496 42-43 21.67758949042581 28.583466123824703 27.78323434405795 21.955710041691535 44-45 21.61219334700807 28.588547728290592 27.707387885910357 22.09187103879098 46-47 22.132351100840125 28.438982990429224 27.509171455593624 21.919494453137027 48-49 21.794257747217554 28.301818103215837 27.75141554028303 22.15250860928358 50-51 21.379955282246687 28.649927341235287 27.926866450460068 22.04325092605795 52-53 21.898637307896212 28.660030807899624 27.589300815400613 21.85203106880355 54-55 21.56473554235509 28.31239495415906 28.043371417823053 22.079498085662795 56-57 21.831818598961085 28.45984013567004 27.538257692684 22.170083572684877 58-59 22.142855517625044 28.13590970903903 27.398894040393085 22.322340732942834 60-61 21.650171824954704 28.234469200822453 28.265868684930446 21.849490289292397 62-63 21.641921225157226 28.19952642867761 28.262477132967057 21.896075213198106 64-65 21.538949275705814 28.464558523554018 27.735884331362264 22.2606078693779 66-67 21.686159881015673 28.7092157485857 27.280311689178475 22.324312681220153 68-69 21.954103453428058 28.047792659313288 27.983779893165423 22.01432399409323 70-71 22.164622792840007 28.002423979497802 27.81706084143033 22.015892386231858 72-73 22.102885642927188 28.07019074048933 27.806215125905105 22.020708490678373 74-75 21.55544246347914 28.824536800726285 27.804053567216513 21.815967168578066 76-77 21.777969242157525 28.12559490266539 28.00466138235091 22.091774472826174 78-79 21.80746636389527 28.164035050794983 27.892056536035824 22.13644204927392 80-81 21.95275211927557 28.352748175379013 27.76165667925427 21.93284302609115 82-83 21.927766767616323 28.33613580496921 28.02684001792975 21.709257409484717 84-85 21.657009154411487 28.31216012256445 28.06445251765277 21.966378205371296 86-87 21.500667435228447 28.59322856622778 27.780550330683816 22.12555366785996 88-89 31.045804276430882 17.869683872485734 33.945490980834535 17.139020870248846 90-91 30.908927295072274 14.772999573201146 16.73442312735575 37.58365000437083 92-93 22.74518251083208 17.314494817311253 34.93627195883586 25.004050713020803 94-95 15.761906199484589 6.885876679959052 27.66728234289964 49.684934777656714 96-97 27.38928526816221 7.167672106574181 15.088211737561814 50.354830887701794 98-99 57.616817340159024 14.051301601132774 18.766474240278836 9.565406818429365 100-101 31.217051929765145 7.016508139604169 12.43073261141583 49.33570731921486 102-103 23.770596407340395 2.0282211165675883 36.764264021711035 37.43691845438098 104-105 42.957606345932945 4.504985271815652 39.41470165251133 13.122706729740077 106-107 5.770885921804083 1.780182084247504 6.768613480673931 85.68031851327447 108-109 18.892783014938637 10.645295926584106 20.9716620619759 49.49025899650136 110-111 40.610543716641736 2.626305912162478 24.776718839828664 31.986431531367117 112-113 27.88394612868031 2.7480788176561535 38.476974648372476 30.891000405291063 114-115 28.66070144143626 3.922375348850591 23.27790085450413 44.13902235520902 116-117 19.830414362985184 4.1884124020523075 20.23357040190077 55.74760283306174 118-119 20.50445479601156 4.2950322356421395 15.672341468802959 59.52817149954333 120-121 25.597437126118745 4.2386734268004265 29.758242777425686 40.40564666965514 122-123 34.397756378063484 1.6055017075130573 33.78851320811571 30.208228706307754 124-125 38.6992833395799 1.8392210357966037 19.261898945266445 40.19959667935705 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 31.0 1 75.0 2 148.0 3 203.5 4 241.0 5 255.0 6 250.5 7 225.5 8 206.0 9 181.0 10 166.0 11 156.5 12 143.0 13 151.5 14 170.5 15 181.0 16 216.5 17 278.0 18 372.5 19 544.0 20 804.0 21 1227.5 22 1867.5 23 2843.0 24 4218.0 25 5950.5 26 8045.5 27 10527.5 28 13509.5 29 17066.5 30 21396.5 31 26334.5 32 31663.0 33 37048.5 34 41969.5 35 46213.0 36 49086.5 37 50894.0 38 52071.5 39 52950.0 40 53449.5 41 53222.5 42 53015.5 43 53220.0 44 53757.5 45 53491.0 46 51693.0 47 49291.5 48 47133.0 49 45059.5 50 42856.5 51 40048.0 52 36567.5 53 32777.0 54 28961.5 55 25585.5 56 22381.0 57 19077.5 58 15940.5 59 13292.5 60 10942.0 61 8767.5 62 6981.0 63 5442.0 64 4014.0 65 2946.5 66 2235.0 67 1680.5 68 1252.0 69 962.5 70 718.5 71 482.0 72 327.0 73 245.5 74 201.0 75 164.0 76 135.5 77 98.0 78 55.0 79 29.0 80 18.5 81 10.5 82 9.5 83 12.5 84 10.5 85 7.5 86 5.0 87 6.0 88 7.0 89 4.5 90 3.0 91 5.5 92 5.0 93 2.0 94 2.0 95 2.5 96 3.5 97 5.5 98 5.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 5.309091515844225E-4 3 0.040576628013952294 4 0.04672000533942918 5 0.04694753783296536 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.72207891888694E-4 12-13 0.0018581820305454788 14-15 3.792208225603018E-5 16-17 5.309091515844225E-4 18-19 0.007887793109254276 20-21 0.0018202599482894488 22-23 5.688312338404526E-4 24-25 0.0 26-27 0.0 28-29 0.0011755845499369358 30-31 0.0 32-33 0.0013272728789610563 34-35 1.5168832902412072E-4 36-37 1.5168832902412072E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 2.6545457579221126E-4 46-47 0.0 48-49 2.6545457579221126E-4 50-51 7.584416451206036E-5 52-53 0.0 54-55 0.0015927274547532675 56-57 0.0 58-59 0.0 60-61 0.0 62-63 7.584416451206036E-5 64-65 1.896104112801509E-4 66-67 0.0 68-69 4.550649870723622E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 4.929870693283923E-4 80-81 0.0 82-83 0.001061818303168845 84-85 2.275324935361811E-4 86-87 9.859741386567845E-4 88-89 19.252548174317194 90-91 26.252547415875547 92-93 40.31834071170647 94-95 44.72947524181016 96-97 48.39070059530084 98-99 93.03674725614773 100-101 51.630687459091554 102-103 53.049428400454154 104-105 74.84423504713335 106-107 81.62671322487111 108-109 12.593544296405062 110-111 39.116589925012875 112-113 51.99985892985401 114-115 86.8194218702716 116-117 66.46652655721343 118-119 71.84869392556502 120-121 80.37365386088511 122-123 75.83665593977366 124-125 50.01649610578137 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1318493.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.29740211335932 #Duplication Level Percentage of deduplicated Percentage of total 1 74.63219829001059 40.523344811566716 2 14.055240726616141 15.263261150662824 3 4.948325733783028 8.060436964653025 4 2.205130254374352 4.789313765363939 5 1.1449048762345886 3.1082680223227666 6 0.6905146388274688 2.2495890605745688 7 0.4420053468233551 1.679981943690579 8 0.30971991364886103 1.3453589355125706 9 0.21714816923820002 1.0611523316975713 >10 1.2040157132021354 12.228238481956012 >50 0.10027049488182331 3.7957454107967594 >100 0.04759480208229371 4.575518549637501 >500 0.002512320237577836 0.8952446753890978 >1k 4.187200395963061E-4 0.4245458961762233 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2847 0.21592833636583583 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1591 0.12066806573868803 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.1376624676809053E-4 0.0 20-21 0.0 0.0 0.0 1.8961041128015088E-4 0.0 22-23 0.0 0.0 0.0 3.0337665804824144E-4 0.0 24-25 0.0 0.0 0.0 4.5506498707236214E-4 0.0 26-27 0.0 0.0 0.0 0.0010238962209128148 0.0 28-29 3.792208225603018E-5 0.0 0.0 0.001896104112801509 0.0 30-31 7.584416451206036E-5 0.0 0.0 0.004550649870723622 0.0 32-33 7.584416451206036E-5 0.0 0.0 0.009177143905959303 0.0 34-35 7.584416451206036E-5 0.0 0.0 0.01448623542180353 0.0 36-37 7.584416451206036E-5 0.0 0.0 0.024801041795443735 0.0 38-39 7.584416451206036E-5 0.0 0.0 0.04459636873309149 0.0 40-41 7.584416451206036E-5 0.0 0.0 0.0689802676237189 0.0 42-43 7.584416451206036E-5 0.0 0.0 0.09397091983044278 0.0 44-45 7.584416451206036E-5 0.0 0.0 0.12222287111118527 0.0 46-47 7.584416451206036E-5 0.0 0.0 0.15684573221094084 0.0 48-49 7.584416451206036E-5 0.0 0.0 0.1956400223588597 0.0 50-51 7.584416451206036E-5 0.0 0.0 0.2426254822740811 0.0 52-53 7.584416451206036E-5 0.0 0.0 0.2882078251458294 0.0 54-55 7.584416451206036E-5 0.0 0.0 0.336786012515804 0.0 56-57 7.584416451206036E-5 0.0 0.0 0.3916971876225357 0.0 58-59 7.584416451206036E-5 0.0 0.0 0.4582504419818687 0.0 60-61 7.584416451206036E-5 0.0 0.0 0.5351943468793539 0.0 62-63 7.584416451206036E-5 0.0 0.0 0.610621368486598 0.0 64-65 7.584416451206036E-5 0.0 0.0 0.6901818970597493 0.0 66-67 7.584416451206036E-5 0.0 0.0 0.7817258036258061 0.0 68-69 7.584416451206036E-5 0.0 0.0 0.8835845165655032 0.0 70-71 7.584416451206036E-5 0.0 0.0 1.011647388344117 0.0 72-73 7.584416451206036E-5 0.0 0.0 1.1389518184776104 0.0 74-75 7.584416451206036E-5 0.0 0.0 1.2635258586886695 0.0 76-77 7.584416451206036E-5 0.0 0.0 1.3994006794120257 0.0 78-79 7.584416451206036E-5 0.0 0.0 1.433833930100501 0.0 80-81 7.584416451206036E-5 0.0 0.0 1.4345923717456217 0.0 82-83 7.584416451206036E-5 0.0 0.0 1.4345923717456217 0.0 84-85 7.584416451206036E-5 0.0 0.0 1.4345923717456217 0.0 86-87 7.584416451206036E-5 0.0 0.0 1.4345923717456217 0.0 88-89 7.584416451206036E-5 0.0 0.0 1.4345923717456217 0.0 90-91 7.584416451206036E-5 0.0 0.0 1.4345923717456217 0.0 92-93 7.584416451206036E-5 0.0 0.0 1.4345923717456217 0.0 94-95 7.584416451206036E-5 0.0 0.0 1.4345923717456217 0.0 96-97 7.584416451206036E-5 0.0 0.0 1.4345923717456217 0.0 98-99 7.584416451206036E-5 0.0 0.0 1.4345923717456217 0.0 100-101 7.584416451206036E-5 0.0 0.0 1.4345923717456217 0.0 102-103 7.584416451206036E-5 0.0 0.0 1.4345923717456217 0.0 104-105 7.584416451206036E-5 0.0 0.0 1.4345923717456217 0.0 106-107 7.584416451206036E-5 0.0 0.0 1.4345923717456217 0.0 108-109 7.584416451206036E-5 0.0 0.0 1.4345923717456217 0.0 110-111 7.584416451206036E-5 0.0 0.0 1.4345923717456217 0.0 112-113 7.584416451206036E-5 0.0 0.0 1.4345923717456217 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCTCC 490 0.0 2090.4714 108-109 CGTCGGT 20 0.0035447637 1517.8259 110-111 CTCCGCC 255 0.0 1428.542 110-111 CGTCGCC 190 0.0 1051.9446 108-109 CGTCGCG 55 2.8892646E-5 908.49756 108-109 CGGCGCG 95 1.4040597E-7 788.95844 108-109 CGTATCT 80 4.9479022E-5 758.91296 110-111 CCGCGCC 585 0.0 726.02155 108-109 CGGCGCC 250 0.0 699.5431 108-109 TTGTTCG 190 3.6379788E-12 657.46533 108-109 TGTCGCG 50 0.005007817 607.1304 110-111 CATCGCG 100 1.7342692E-4 499.67365 108-109 CCGTTCG 105 2.007334E-4 475.87973 108-109 CCTCTCG 265 3.092282E-11 471.3903 108-109 CTGCGCG 55 0.0020700172 454.24878 108-109 GGTCTCG 135 2.3744725E-4 449.72623 110-111 CGTGGTT 150 3.2559672E-4 404.75357 110-111 CGCTGTC 150 3.2559672E-4 404.75357 110-111 TTTTTCG 810 0.0 402.06235 92-93 CGTGTCT 235 2.3976463E-6 387.53003 110-111 >>END_MODULE