FastQCFastQC Report
Fri 27 May 2016
SRR939970_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939970_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1318493
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51110.3876395248211405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33890.2570358735313726No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29580.22434703862667457No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20110.15252261483375337No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15740.119378714941983No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA113850.032.1324841
TATCAAC131300.027.719572
TCAACGC131400.027.6531414
ATCAACG131500.027.632113
CAACGCA133050.027.3549775
GTACATG85650.027.1297741
AGTACTT58950.027.09836812-13
AACGCAG134350.027.0574076
GAGTACT59400.027.04331612-13
GTACTTT60750.026.49232514-15
TACATGG87550.025.9906542
GGTATCA82800.025.329191
ACATGGG86200.025.153833
ACGCAGA147500.024.5644997
CGCAGAG148300.024.351758
AGAGTAC113100.024.040110-11
CATGGGG31750.023.4518054
GCAGAGT157350.022.7242939
GTCCTAC15550.022.2231981
GTATAGG4900.021.8869551
Produced by FastQC (version 0.11.5)