##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939970_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1318493 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.83801658408501 34.0 31.0 34.0 31.0 34.0 2 32.906508415289274 34.0 31.0 34.0 31.0 34.0 3 32.940992481567974 34.0 33.0 34.0 31.0 34.0 4 36.35999129308991 37.0 37.0 37.0 35.0 37.0 5 36.26217355723542 37.0 37.0 37.0 35.0 37.0 6 36.39451100612593 37.0 37.0 37.0 35.0 37.0 7 36.38646242338791 37.0 37.0 37.0 35.0 37.0 8 36.408624088258335 37.0 37.0 37.0 35.0 37.0 9 38.23505850998071 39.0 39.0 39.0 37.0 39.0 10-11 38.2301953821522 39.0 39.0 39.0 37.0 39.0 12-13 38.21880283020084 39.0 39.0 39.0 37.0 39.0 14-15 39.73803273889205 41.0 40.0 41.0 37.0 41.0 16-17 39.761985842928254 41.0 40.0 41.0 37.0 41.0 18-19 39.82355158502927 41.0 40.0 41.0 37.5 41.0 20-21 39.839717389474195 41.0 40.0 41.0 38.0 41.0 22-23 39.78860221480129 41.0 40.0 41.0 37.0 41.0 24-25 39.74597172681236 41.0 40.0 41.0 37.0 41.0 26-27 39.65176417318864 41.0 40.0 41.0 37.0 41.0 28-29 39.56541369578754 41.0 40.0 41.0 37.0 41.0 30-31 39.429972703685195 41.0 39.0 41.0 36.0 41.0 32-33 39.26386601976651 41.0 39.0 41.0 35.0 41.0 34-35 39.15558330609264 41.0 39.0 41.0 35.0 41.0 36-37 39.133416711351515 41.0 39.0 41.0 35.0 41.0 38-39 39.18357624955157 41.0 39.0 41.0 35.0 41.0 40-41 39.11889293306828 41.0 39.0 41.0 35.0 41.0 42-43 38.987078808912905 41.0 39.0 41.0 35.0 41.0 44-45 38.806473375285265 41.0 39.0 41.0 35.0 41.0 46-47 38.56068215758445 41.0 39.0 41.0 35.0 41.0 48-49 38.2972556547513 41.0 39.0 41.0 35.0 41.0 50-51 38.12747318339953 41.0 39.0 41.0 34.5 41.0 52-53 38.00888590231423 41.0 38.5 41.0 34.0 41.0 54-55 37.8790964381305 41.0 38.0 41.0 34.0 41.0 56-57 37.725654971243685 40.5 38.0 41.0 34.0 41.0 58-59 37.55556874401305 40.0 37.0 41.0 34.0 41.0 60-61 37.35843876304236 40.0 37.0 41.0 33.0 41.0 62-63 37.1364774026104 40.0 36.5 41.0 33.0 41.0 64-65 36.87778585096773 39.0 36.0 41.0 33.0 41.0 66-67 36.59967250489764 39.0 35.5 41.0 32.5 41.0 68-69 36.267167137026895 39.0 35.0 41.0 32.0 41.0 70-71 35.906186456810914 37.5 35.0 40.0 32.0 41.0 72-73 35.54974656672428 37.0 35.0 39.5 31.5 41.0 74-75 35.1834389716138 36.5 35.0 39.0 31.0 41.0 76-77 34.79933150953399 36.0 35.0 39.0 31.0 40.5 78-79 34.4314922415212 36.0 35.0 37.5 31.0 39.5 80-81 34.14829619876632 35.0 35.0 37.0 31.0 39.0 82-83 33.9092353922243 35.0 35.0 37.0 31.0 39.0 84-85 33.649927606744974 35.0 35.0 36.0 31.0 37.0 86-87 33.445651209373125 35.0 35.0 36.0 31.0 37.0 88-89 33.255220922674596 35.0 35.0 36.0 31.0 37.0 90-91 33.10700208495608 35.0 35.0 35.0 31.0 36.0 92-93 32.97149548765143 35.0 34.5 35.0 30.0 36.0 94-95 32.88053444348965 35.0 34.0 35.0 30.0 36.0 96-97 32.8114191732531 35.0 34.0 35.0 30.0 36.0 98-99 32.740855658695196 35.0 34.0 35.0 30.0 36.0 100-101 32.697290391378644 35.0 34.0 35.0 30.0 35.5 102-103 31.87412523236756 34.5 32.5 35.0 28.0 35.0 104-105 32.5434837348397 35.0 34.0 35.0 29.5 35.0 106-107 32.60647610567519 35.0 34.0 35.0 30.0 35.0 108-109 32.610809082793764 35.0 34.0 35.0 30.0 35.0 110-111 32.5861908254348 35.0 34.0 35.0 30.0 35.0 112-113 32.52814652789207 35.0 34.0 35.0 30.0 35.0 114-115 32.484394304709994 35.0 34.0 35.0 29.5 35.0 116-117 32.43213047016556 35.0 34.0 35.0 29.0 35.0 118-119 32.36183013485851 35.0 34.0 35.0 29.0 35.0 120-121 32.28142773605927 35.0 34.0 35.0 29.0 35.0 122-123 32.12772233147996 35.0 34.0 35.0 29.0 35.0 124-125 30.17426524069525 33.5 30.5 34.5 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03711456863795348 1101 2 0.11364387359729733 1101 3 0.08157629544130174 1101 4 -0.004150965636256387 1101 5 -0.06472712914297318 1101 6 0.0015497692187125267 1101 7 0.03944409740098109 1101 8 0.08399970366937737 1101 9 0.09995966729182015 1101 10-11 0.10688903595726629 1101 12-13 0.12695154416512366 1101 14-15 0.20870197501448473 1101 16-17 0.1774118435558094 1101 18-19 0.19850576225450567 1101 20-21 0.19817039837488437 1101 22-23 0.19466145377968758 1101 24-25 0.2744638875901231 1101 26-27 0.1782168501625705 1101 28-29 0.2010373797799403 1101 30-31 0.198800354308581 1101 32-33 0.25206632871727663 1101 34-35 0.30452471347221177 1101 36-37 0.26428736548199794 1101 38-39 0.13769043419921445 1101 40-41 0.23656064083684214 1101 42-43 0.17514740028958187 1101 44-45 0.2522468680931098 1101 46-47 0.36838778611326717 1101 48-49 0.21884406409265011 1101 50-51 0.23639172880445614 1101 52-53 0.21986566995698098 1101 54-55 0.3241533748640322 1101 56-57 0.28242712884591725 1101 58-59 0.22454945657737113 1101 60-61 0.25314808702731284 1101 62-63 0.1861597169431306 1101 64-65 0.23217222426031014 1101 66-67 0.20953101183990697 1101 68-69 0.2263452231525207 1101 70-71 0.27455992766181936 1101 72-73 0.2845811249744514 1101 74-75 0.28783508894313314 1101 76-77 0.28870083800229907 1101 78-79 0.30141805388229415 1101 80-81 0.35671058955411183 1101 82-83 0.3166634066027072 1101 84-85 0.17203986380589242 1101 86-87 0.16162681183455163 1101 88-89 0.13657254996323331 1101 90-91 0.210482630268217 1101 92-93 0.18719486365126414 1101 94-95 0.2057124973145079 1101 96-97 0.22782311087243556 1101 98-99 0.23471558379908686 1101 100-101 0.28608028155328924 1101 102-103 0.33339183236384784 1101 104-105 0.2639511292446315 1101 106-107 0.23716251415266498 1101 108-109 0.26263425860138057 1101 110-111 0.25563349155039816 1101 112-113 0.30155680049363554 1101 114-115 0.24257724967021232 1101 116-117 0.36410099616112745 1101 118-119 0.41012894348838813 1101 120-121 0.4180039257141672 1101 122-123 0.3693345562030075 1101 124-125 0.4756241862024311 1102 1 -0.04631121766670532 1102 2 0.053482680356260914 1102 3 -0.011251381308156283 1102 4 -0.07752681685023788 1102 5 -0.08090098127554768 1102 6 -0.06699825424512085 1102 7 -0.054518181786718856 1102 8 8.801132168159143E-4 1102 9 -0.04651100606641734 1102 10-11 0.06228615729681053 1102 12-13 0.012411225931160175 1102 14-15 0.06170279922660882 1102 16-17 0.11953663304615958 1102 18-19 0.01039979332290386 1102 20-21 -0.11270857247149024 1102 22-23 0.05928828282802101 1102 24-25 0.042385258173091245 1102 26-27 -0.014758891156461118 1102 28-29 -0.004824802725373445 1102 30-31 -0.010701574096124489 1102 32-33 -0.02501365341430528 1102 34-35 0.007216930015594869 1102 36-37 0.03450101049195098 1102 38-39 0.01623134804702886 1102 40-41 -0.13842965946578545 1102 42-43 -0.1780253969353609 1102 44-45 -0.09636549844429254 1102 46-47 0.12473872850440415 1102 48-49 0.09195419602950494 1102 50-51 0.039632581203370876 1102 52-53 0.03735406702168831 1102 54-55 0.1374273844987144 1102 56-57 0.021560846252306476 1102 58-59 -0.015758710672677978 1102 60-61 0.08282454706770892 1102 62-63 0.11261084410039501 1102 64-65 0.08003692763831793 1102 66-67 0.13378528861998262 1102 68-69 -0.06230204132309325 1102 70-71 0.020611364370502372 1102 72-73 0.07132095707006414 1102 74-75 0.028667564159100323 1102 76-77 0.07205783510183039 1102 78-79 0.11885297153448704 1102 80-81 0.0739376715286113 1102 82-83 0.053022486253475165 1102 84-85 0.047724347855393034 1102 86-87 0.015788453883210707 1102 88-89 0.03058264603743055 1102 90-91 0.11808908051454381 1102 92-93 0.12244178124473848 1102 94-95 0.05473172683346661 1102 96-97 0.026241378600865062 1102 98-99 0.08686702910642197 1102 100-101 0.12640648499736784 1102 102-103 0.15476849730378817 1102 104-105 0.21313310395620988 1102 106-107 0.059614010422741615 1102 108-109 0.12344936873751067 1102 110-111 0.05990513341030379 1102 112-113 0.21677620240505036 1102 114-115 0.13865731359384625 1102 116-117 0.18770387272435585 1102 118-119 0.15398143258802577 1102 120-121 0.1579712927863639 1102 122-123 0.11997683346125854 1102 124-125 0.173141483122226 1103 1 -0.00881644198091891 1103 2 0.09894928624855481 1103 3 0.07156903480210275 1103 4 0.030310601035196783 1103 5 0.01531202803138143 1103 6 0.06761182624208573 1103 7 0.003917538396827069 1103 8 -0.004495214622551202 1103 9 0.015280799005140011 1103 10-11 0.08203413783542146 1103 12-13 0.09621286654785877 1103 14-15 0.03741654612660028 1103 16-17 0.07429982416649494 1103 18-19 0.03095002464897334 1103 20-21 0.09336255392705084 1103 22-23 0.05204283253218733 1103 24-25 0.15521165949071047 1103 26-27 0.10763175502749078 1103 28-29 0.08289110525679888 1103 30-31 -0.006179320337892591 1103 32-33 0.014237511225495325 1103 34-35 -0.03959379908750549 1103 36-37 0.0592336056982532 1103 38-39 0.008553681316776363 1103 40-41 -0.022508134126226764 1103 42-43 -0.028911163937500817 1103 44-45 0.009516897327905838 1103 46-47 -0.11351592095321195 1103 48-49 -0.24204105366722928 1103 50-51 -0.21826447031746454 1103 52-53 -0.2624438048074964 1103 54-55 -0.21452246790985185 1103 56-57 -0.1966469991749804 1103 58-59 -0.264021617095203 1103 60-61 -0.3314059751598464 1103 62-63 -0.35568211942187844 1103 64-65 -0.37598971017509086 1103 66-67 -0.25518154226369205 1103 68-69 -0.19285776272989352 1103 70-71 -0.20952670315021038 1103 72-73 -0.24567208057558076 1103 74-75 -0.22604130865597227 1103 76-77 -0.14874971645322432 1103 78-79 -0.19845403465313183 1103 80-81 -0.26321313676564273 1103 82-83 -0.26849309640136454 1103 84-85 -0.2644015948875733 1103 86-87 -0.15957957811944823 1103 88-89 -0.1742398874673583 1103 90-91 -0.15702061188075334 1103 92-93 -0.1949756090592274 1103 94-95 -0.1814512544987963 1103 96-97 -0.21457103684534218 1103 98-99 -0.1711794341602726 1103 100-101 -0.116330516836058 1103 102-103 -0.19039156694617176 1103 104-105 -0.1367940104894032 1103 106-107 -0.15527473898929856 1103 108-109 -0.1671330312578334 1103 110-111 -0.08663895543288902 1103 112-113 -0.07075679997009132 1103 114-115 -0.1468226607178238 1103 116-117 -0.16609527769657717 1103 118-119 -0.24432363241191268 1103 120-121 -0.2944327655046024 1103 122-123 -0.22943054363447501 1103 124-125 -0.10728033565268547 1104 1 -0.03943361960527625 1104 2 0.061228245534003634 1104 3 0.022642115411642294 1104 4 0.01442900801404079 1104 5 -0.016610904638888258 1104 6 -0.021137843846403825 1104 7 -0.015518764371648786 1104 8 -0.049470141347470076 1104 9 -0.06549820889884472 1104 10-11 -0.03594638696698382 1104 12-13 0.010248344190273428 1104 14-15 -0.012933507353785956 1104 16-17 -0.0033828773573461035 1104 18-19 0.04935769804352219 1104 20-21 0.08648650341933717 1104 22-23 0.05925296185750284 1104 24-25 0.07153012481179388 1104 26-27 0.04100560380994267 1104 28-29 0.01456261062567421 1104 30-31 0.014333535792907526 1104 32-33 -0.01975448755209186 1104 34-35 0.0067229993122452925 1104 36-37 0.008739780252696505 1104 38-39 -0.033107544703874225 1104 40-41 -0.05948461664438298 1104 42-43 -0.014635371802590669 1104 44-45 -0.018053981556370502 1104 46-47 -0.029572540714568163 1104 48-49 -0.02629281435288533 1104 50-51 -0.03866064195479879 1104 52-53 -0.10177481371163566 1104 54-55 0.01784844986925549 1104 56-57 -0.08833183991453808 1104 58-59 -0.14334762523532874 1104 60-61 -0.1141109717565385 1104 62-63 -0.03000805810695084 1104 64-65 -0.052218717384519664 1104 66-67 -0.09243792865606082 1104 68-69 -0.08794520664493888 1104 70-71 0.03856328281987231 1104 72-73 -0.07426603242933538 1104 74-75 -0.11183010972279561 1104 76-77 -0.01221982994279358 1104 78-79 -0.06339451167045951 1104 80-81 -0.08362875581855178 1104 82-83 -0.03959979325782825 1104 84-85 -0.14508058751144404 1104 86-87 -0.1344806046651712 1104 88-89 -0.07767443996843326 1104 90-91 -0.10895285152847123 1104 92-93 -0.07378070428210748 1104 94-95 -0.0889822661811408 1104 96-97 -0.0535999162574754 1104 98-99 -0.03554968517084234 1104 100-101 -0.06326754888262087 1104 102-103 -0.09739888750904058 1104 104-105 -0.04136068039729679 1104 106-107 -0.044257083514153805 1104 108-109 -0.03336884506271076 1104 110-111 -0.08582285852919824 1104 112-113 -0.07147218704312053 1104 114-115 -0.026020871555594738 1104 116-117 -0.051422897194001393 1104 118-119 -0.019985886367379635 1104 120-121 -0.11583672999918093 1104 122-123 -0.0525271278348427 1104 124-125 -0.1374655028310059 1105 1 0.02550813993251211 1105 2 0.0344429335267975 1105 3 -0.02253764155535265 1105 4 0.05255232672651999 1105 5 -0.06948052286239204 1105 6 -0.06330155321390407 1105 7 -0.019777805226105727 1105 8 -0.015293127446618371 1105 9 -0.03761169497811778 1105 10-11 0.05279379033845544 1105 12-13 0.035622368546583516 1105 14-15 -0.012540599571160271 1105 16-17 0.022098484369593052 1105 18-19 -0.009459868180982767 1105 20-21 0.012883545678462838 1105 22-23 0.054206117708716306 1105 24-25 -0.05230738496120324 1105 26-27 0.02397672873210155 1105 28-29 -0.028135135289545588 1105 30-31 -5.678065868011117E-4 1105 32-33 -0.02317669446144066 1105 34-35 -0.004514295786016476 1105 36-37 0.031035474831497822 1105 38-39 0.0339988518083274 1105 40-41 0.016836229333108577 1105 42-43 0.08774990310033814 1105 44-45 -0.1005288367834325 1105 46-47 -0.026355069901050854 1105 48-49 0.07173403324129879 1105 50-51 0.06481390848713886 1105 52-53 -0.02234383429125586 1105 54-55 -0.07393361551378774 1105 56-57 0.05951544948556631 1105 58-59 -0.013609872959385427 1105 60-61 0.07077102339339802 1105 62-63 0.13493038854902295 1105 64-65 0.16024103110149213 1105 66-67 0.05462420775516108 1105 68-69 0.039393356624479736 1105 70-71 0.19555478859091124 1105 72-73 0.08012552369751091 1105 74-75 -0.04570697653170441 1105 76-77 0.05168499758858047 1105 78-79 0.0275136154911948 1105 80-81 -0.0780366113238955 1105 82-83 -0.004701896275200568 1105 84-85 -0.19852664716982815 1105 86-87 -0.14714840212882052 1105 88-89 -0.11403199267638797 1105 90-91 -0.02104288240452945 1105 92-93 -0.061085741187618225 1105 94-95 -0.04771634778404632 1105 96-97 -0.1085652635137464 1105 98-99 -0.1376056596219044 1105 100-101 -0.08515178354066677 1105 102-103 -0.050569977977215075 1105 104-105 0.026922451733675246 1105 106-107 -0.05976998387591692 1105 108-109 0.00359165213458823 1105 110-111 -0.038405581187987536 1105 112-113 -0.15141202510945817 1105 114-115 -0.038258656960358906 1105 116-117 -0.05089879929171559 1105 118-119 0.008400872706602058 1105 120-121 -0.17737881022392799 1105 122-123 -0.19680710610233376 1105 124-125 -0.27816889389682586 1106 1 0.0281139549406646 1106 2 0.05059452287671462 1106 3 0.05161454727994652 1106 4 0.04612407876795288 1106 5 -0.025454420594336113 1106 6 0.06284729822661461 1106 7 0.011728654812742434 1106 8 0.03319771353275058 1106 9 0.06319267944851248 1106 10-11 0.059831774614288236 1106 12-13 0.11467969725531191 1106 14-15 -0.03343084833164056 1106 16-17 0.10292779842597355 1106 18-19 0.0774851050303198 1106 20-21 0.10329735927484052 1106 22-23 0.11326554850902681 1106 24-25 0.09091732733123337 1106 26-27 0.18855609631395254 1106 28-29 0.15659329913233933 1106 30-31 0.08481720705733409 1106 32-33 0.14794095649014594 1106 34-35 0.11741350385611327 1106 36-37 0.07220221351425948 1106 38-39 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2216 92-93 -0.07655197613244269 2216 94-95 -0.19095267584835085 2216 96-97 -0.20191939721108554 2216 98-99 -0.1487692835567529 2216 100-101 -0.11229379424013786 2216 102-103 -0.06348038248072285 2216 104-105 -0.127711075781626 2216 106-107 -0.18638977115076472 2216 108-109 -0.15814803545280398 2216 110-111 -0.15550438041697134 2216 112-113 -0.1390222368595957 2216 114-115 -0.1658812117836348 2216 116-117 -0.2892570419895364 2216 118-119 -0.10438095811829129 2216 120-121 -0.16117981245574953 2216 122-123 -0.21052934624153608 2216 124-125 -0.23241873612620623 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 109.0 3 12.0 4 22.0 5 44.0 6 136.0 7 248.0 8 559.0 9 936.0 10 2048.0 11 2812.0 12 3800.0 13 6135.0 14 10618.0 15 11307.0 16 4354.0 17 2890.0 18 2625.0 19 2229.0 20 2108.0 21 2078.0 22 2187.0 23 2324.0 24 2798.0 25 3566.0 26 4554.0 27 6038.0 28 7522.0 29 9696.0 30 12713.0 31 16022.0 32 20734.0 33 27995.0 34 40675.0 35 68679.0 36 158361.0 37 498697.0 38 378393.0 39 2469.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.01056812389252 20.71456962885647 15.533670534262203 21.74119171298881 2 14.056654074007218 21.71008871491923 41.276973029056656 22.95628418201689 3 18.25280831980147 27.892829161777875 29.74676391911068 24.10759859930997 4 11.816824207637053 18.64568109197394 39.598996733391836 29.93849796699717 5 12.788587275960895 39.018381800149754 34.108696081394484 14.084334842494869 6 28.482820917517195 38.57692077242731 19.215119079130492 13.725139230925004 7 26.699345389016095 33.81087347448943 22.47725243895872 17.012528697535746 8 25.56016603804495 34.99366322005501 21.017934869582167 18.428235872317867 9 26.45952614082896 17.383179129506185 21.8903702939644 34.26692443570046 10-11 23.936759618746553 27.782893045317646 28.168939842684033 20.111407493251765 12-13 25.78493021957644 25.254551977143603 28.64603755954715 20.314480243732806 14-15 22.228824878099466 26.831579689842872 27.405568326870146 23.534027105187512 16-17 20.595202926981326 29.73071877975702 28.433175337118215 21.24090295614344 18-19 20.825404457968304 27.790363695522085 31.180825381704718 20.203406464804893 20-21 22.225824973055218 26.8824752339323 30.47790679418129 20.41379299883119 22-23 21.448656626649946 27.35389447250473 30.39214161952627 20.805307281319056 24-25 21.380052833045 27.240265970316113 30.91017548064343 20.46950571599546 26-27 20.767687048774626 27.527070678418468 31.00319834841747 20.702043924389436 28-29 20.57409481885759 27.61558081840404 30.644379606110917 21.165944756627454 30-31 20.948234082395583 27.11284777393585 30.82299261353682 21.115925530131747 32-33 20.820588353521785 27.467457165111988 30.671835193664283 21.040119287701945 34-35 21.39484244512485 27.40609923602173 30.640094410815983 20.558963908037434 36-37 21.227643984458012 26.90514852941957 30.717455458618286 21.149752027504128 38-39 21.189456447626192 27.46476469727181 30.35021801405089 20.99556084105111 40-41 21.68058533492404 27.06047737834027 30.535770762529644 20.723166524206043 42-43 21.184640343179677 27.429042095786627 30.119234611029412 21.267082950004284 44-45 21.153013326578147 27.50898184518234 29.853931723566223 21.48407310467329 46-47 21.733638328000225 27.45152989056445 29.328635040155692 21.486196741279628 48-49 21.651688708244944 27.527146522582978 29.108838651399743 21.712326117772335 50-51 21.302995161900746 27.981718522586014 29.02192123886892 21.69336507664432 52-53 21.703338584277656 28.127946071765265 28.6067502823299 21.561965061627177 54-55 21.39745906880052 27.838373051658216 28.92575083826763 21.838417041273637 56-57 21.768033656606445 27.875764224762666 28.47588117646434 21.88032094216655 58-59 21.997120955515122 27.559456136665116 28.42335909253974 22.020063815280018 60-61 21.55320506062603 27.664841603254626 29.270386721810432 21.51156661430891 62-63 21.55362220353085 27.509209377675877 29.322112442007658 21.61505597678562 64-65 21.383048677543226 27.694458749496587 28.94175395697967 21.980738615980517 66-67 21.561585840804614 28.079898793546874 28.402236492723134 21.956278872925377 68-69 21.8284435336403 27.415731444914766 29.062004879813546 21.69382014163139 70-71 22.033033167411585 27.418916899824268 28.841867192317288 21.70618274044686 72-73 21.959047184930068 27.522368340218716 28.784187705205866 21.734396769645343 74-75 21.477019597373666 28.201704521753246 28.746265622949835 21.57501025792325 76-77 21.657945851817185 27.571818735480587 28.91448797983759 21.855747432864643 78-79 21.65544299438829 27.62581978061317 28.759955494644263 21.958781730354275 80-81 21.790369763055246 27.82946136232805 28.6054609315332 21.774707943083506 82-83 21.79529963374853 27.812130970737048 28.783998094794587 21.608571300719838 84-85 21.564429996973818 27.78725408477709 28.76469575492627 21.883620163322824 86-87 21.383882963352857 27.982932029218205 28.516533648642806 22.11665135878613 88-89 21.6111879243955 27.70196732178328 28.421045845522126 22.265798908299097 90-91 21.657895864532346 27.68200915520812 28.75183591464291 21.908259065616623 92-93 21.68104039991111 27.91448267074607 28.741146141845274 21.663330787497546 94-95 21.285547047872875 27.88939397329428 28.680379664030177 22.144679314802673 96-97 21.63564766745064 27.598022894319502 28.69893886429431 22.067390573935548 98-99 21.595544256331923 27.630836464468878 28.44575336107239 22.32786591812681 100-101 21.29846874249853 27.568361267402935 28.79844124895762 22.334728741140918 102-103 21.596442288439484 27.538703423921945 28.547434637251158 22.317419650387414 104-105 21.69635015237405 27.261908915360728 28.54908659002204 22.49265434224318 106-107 21.62883647656824 27.272285970979514 28.60130755639259 22.497569996059656 108-109 21.410382283019207 27.54806363205084 28.283641887876744 22.757912197053212 110-111 21.30997212583196 27.77689288355424 28.415230293721677 22.497904696892125 112-113 21.412984116870703 27.39422624736419 28.38248456438963 22.810305071375474 114-115 21.063731426094428 27.650994697802023 28.136387959637915 23.148885916465638 116-117 21.35714933109943 27.576860898185355 27.842661510182833 23.223328260532384 118-119 21.23603516143467 27.418524220889047 28.303741401148287 23.041699216528 120-121 20.944796489910768 27.75670561288145 28.40153661209646 22.89696128511132 122-123 21.113664986569717 27.79027748335104 28.09271010344902 23.003347426630224 124-125 21.071972082930564 27.64292727249974 28.048546940351233 23.23655370421847 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 116.0 1 89.0 2 153.5 3 215.0 4 118.0 5 83.0 6 245.5 7 547.5 8 1013.0 9 1198.5 10 902.0 11 663.5 12 679.0 13 802.5 14 910.5 15 1044.0 16 1160.0 17 1351.0 18 1585.5 19 1740.0 20 1915.5 21 2278.5 22 2813.0 23 3356.5 24 4167.5 25 5331.5 26 6893.0 27 8891.5 28 10920.5 29 13595.5 30 17326.5 31 21592.5 32 26827.0 33 32558.5 34 38222.5 35 42905.0 36 47198.0 37 50706.0 38 51394.0 39 50578.0 40 48737.0 41 47966.0 42 48302.5 43 48301.0 44 48509.0 45 48537.5 46 47501.5 47 48501.0 48 53097.5 49 51634.0 50 46576.5 51 41889.5 52 36655.5 53 34323.5 54 32897.5 55 30188.0 56 26187.5 57 22929.5 58 20757.0 59 18167.0 60 15021.5 61 11837.5 62 9146.5 63 6804.5 64 4892.5 65 3921.5 66 3026.5 67 2219.5 68 1574.0 69 1048.5 70 734.5 71 545.0 72 468.0 73 471.0 74 375.5 75 260.5 76 164.0 77 103.0 78 69.5 79 35.0 80 21.0 81 15.5 82 13.0 83 7.5 84 4.5 85 2.5 86 1.0 87 0.0 88 0.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 1.0 98 6.0 99 7.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014334547092779409 2 0.0 3 0.0 4 0.0 5 0.1249153389513634 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.1376624676809055E-4 18-19 0.0 20-21 0.00424727321267538 22-23 1.896104112801509E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 6.44675398352513E-4 92-93 0.0 94-95 8.72207891888694E-4 96-97 0.0 98-99 0.0011376624676809053 100-101 1.1376624676809055E-4 102-103 3.792208225603018E-4 104-105 0.0020477924418256296 106-107 0.006029611078708799 108-109 4.929870693283923E-4 110-111 0.005157403186820104 112-113 0.00857039058986282 114-115 0.005877922749684678 116-117 0.0017823378660334185 118-119 2.275324935361811E-4 120-121 6.067533160964829E-4 122-123 0.0013272728789610563 124-125 0.001251428714448996 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1318493.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.99409202442642 #Duplication Level Percentage of deduplicated Percentage of total 1 73.12266166174119 38.01946399511569 2 14.778499205185744 15.367892953146805 3 5.248285457848787 8.186395111975463 4 2.389425845423929 4.969441091700587 5 1.2399428446531422 3.2234851184962277 6 0.7434644751436215 2.319345620250561 7 0.50765036072966 1.8476373700407847 8 0.3075338444323873 1.2791954406434547 9 0.2183152811924647 1.0215994338593588 >10 1.2725887243901917 12.390934424817743 >50 0.11254629795571718 3.990076983866225 >100 0.055438732497607494 5.151222148246816 >500 0.0027719242922601304 1.0031008596861535 >1k 7.29453761121087E-4 0.8425167308328877 >5k 1.458907522242174E-4 0.3876927173212289 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5111 0.3876395248211405 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3389 0.2570358735313726 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2958 0.22434703862667457 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2011 0.15252261483375337 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1574 0.119378714941983 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 7.584416451206036E-5 0.0 0.0 0.0 0.0 8 7.584416451206036E-5 0.0 0.0 0.0 0.0 9 7.584416451206036E-5 0.0 0.0 0.0 0.0 10-11 7.584416451206036E-5 0.0 0.0 0.0 0.0 12-13 7.584416451206036E-5 0.0 0.0 0.0 0.0 14-15 7.584416451206036E-5 0.0 0.0 0.0 0.0 16-17 7.584416451206036E-5 0.0 0.0 0.0 0.0 18-19 7.584416451206036E-5 0.0 0.0 1.1376624676809053E-4 0.0 20-21 7.584416451206036E-5 0.0 0.0 1.8961041128015088E-4 0.0 22-23 7.584416451206036E-5 0.0 0.0 3.4129874030427163E-4 0.0 24-25 7.584416451206036E-5 0.0 0.0 5.309091515844225E-4 0.0 26-27 7.584416451206036E-5 0.0 0.0 0.0010997403854248753 0.0 28-29 7.584416451206036E-5 0.0 0.0 0.00208571452408166 0.0 30-31 7.584416451206036E-5 0.0 0.0 0.004778182364259803 0.0 32-33 7.584416451206036E-5 0.0 0.0 0.009480520564007545 0.0 34-35 7.584416451206036E-5 0.0 0.0 0.01475168999759574 0.0 36-37 7.584416451206036E-5 0.0 0.0 0.025142340535748008 0.0 38-39 7.584416451206036E-5 0.0 0.0 0.044899745391139737 0.0 40-41 7.584416451206036E-5 0.0 0.0 0.06939741052853524 0.0 42-43 1.5168832902412072E-4 0.0 0.0 0.09438806273525911 0.0 44-45 1.5168832902412072E-4 0.0 0.0 0.12256416985148955 0.0 46-47 1.5168832902412072E-4 0.0 0.0 0.15711118678673303 0.0 48-49 1.5168832902412072E-4 0.0 0.0 0.19582963277013984 0.0 50-51 1.5168832902412072E-4 0.0 0.0 0.24270132643859316 0.0 52-53 1.5168832902412072E-4 0.0 0.0 0.28843535763936556 0.0 54-55 1.5168832902412072E-4 0.0 0.0 0.33720315542062035 0.0 56-57 1.5168832902412072E-4 0.0 0.0 0.39237978510314425 0.0 58-59 1.5168832902412072E-4 0.0 0.0 0.45942602653180564 0.0 60-61 1.5168832902412072E-4 0.0 0.0 0.5361044768534986 0.0 62-63 1.5168832902412072E-4 0.0 0.0 0.6113039659672065 7.584416451206036E-5 64-65 1.5168832902412072E-4 0.0 0.0 0.6904473516355415 7.584416451206036E-5 66-67 1.5168832902412072E-4 0.0 0.0 0.7822567127773905 7.584416451206036E-5 68-69 1.5168832902412072E-4 0.0 0.0 0.8848738673622082 7.584416451206036E-5 70-71 1.5168832902412072E-4 0.0 0.0 1.0130884274698462 7.584416451206036E-5 72-73 1.5168832902412072E-4 0.0 0.0 1.140923766754924 7.584416451206036E-5 74-75 1.5168832902412072E-4 0.0 0.0 1.2658011836240313 7.584416451206036E-5 76-77 1.5168832902412072E-4 0.0 0.0 1.4028895109795805 7.584416451206036E-5 78-79 1.8961041128015088E-4 0.0 0.0 1.5574219961729034 7.584416451206036E-5 80-81 2.2753249353618107E-4 0.0 0.0 1.7358453931875255 7.584416451206036E-5 82-83 2.2753249353618107E-4 3.792208225603018E-5 0.0 1.92981684392712 7.584416451206036E-5 84-85 2.2753249353618107E-4 7.584416451206036E-5 0.0 2.1211337489087922 7.584416451206036E-5 86-87 2.2753249353618107E-4 7.584416451206036E-5 0.0 2.318214810393381 7.584416451206036E-5 88-89 2.2753249353618107E-4 7.584416451206036E-5 0.0 2.5380111991493317 7.584416451206036E-5 90-91 3.0337665804824144E-4 7.584416451206036E-5 0.0 2.7902309682341886 7.584416451206036E-5 92-93 3.0337665804824144E-4 7.584416451206036E-5 0.0 3.0620943759276686 7.584416451206036E-5 94-95 3.0337665804824144E-4 7.584416451206036E-5 0.0 3.3257666138538466 7.584416451206036E-5 96-97 3.0337665804824144E-4 7.584416451206036E-5 0.0 3.5944824887200766 7.584416451206036E-5 98-99 3.0337665804824144E-4 7.584416451206036E-5 0.0 3.8886061586978466 7.584416451206036E-5 100-101 3.0337665804824144E-4 7.584416451206036E-5 0.0 4.225847236200723 7.584416451206036E-5 102-103 3.792208225603018E-4 7.584416451206036E-5 0.0 4.584893511000817 7.584416451206036E-5 104-105 3.792208225603018E-4 7.584416451206036E-5 0.0 4.942347058346158 7.584416451206036E-5 106-107 3.792208225603018E-4 7.584416451206036E-5 0.0 5.29968683944473 7.584416451206036E-5 108-109 3.792208225603018E-4 7.584416451206036E-5 0.0 5.677049479974486 7.584416451206036E-5 110-111 3.792208225603018E-4 7.584416451206036E-5 0.0 6.094040696461794 7.584416451206036E-5 112-113 3.792208225603018E-4 7.584416451206036E-5 0.0 6.541445422918438 7.584416451206036E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11385 0.0 32.132484 1 TATCAAC 13130 0.0 27.71957 2 TCAACGC 13140 0.0 27.653141 4 ATCAACG 13150 0.0 27.63211 3 CAACGCA 13305 0.0 27.354977 5 GTACATG 8565 0.0 27.129774 1 AGTACTT 5895 0.0 27.098368 12-13 AACGCAG 13435 0.0 27.057407 6 GAGTACT 5940 0.0 27.043316 12-13 GTACTTT 6075 0.0 26.492325 14-15 TACATGG 8755 0.0 25.990654 2 GGTATCA 8280 0.0 25.32919 1 ACATGGG 8620 0.0 25.15383 3 ACGCAGA 14750 0.0 24.564499 7 CGCAGAG 14830 0.0 24.35175 8 AGAGTAC 11310 0.0 24.0401 10-11 CATGGGG 3175 0.0 23.451805 4 GCAGAGT 15735 0.0 22.724293 9 GTCCTAC 1555 0.0 22.223198 1 GTATAGG 490 0.0 21.886955 1 >>END_MODULE