FastQCFastQC Report
Fri 27 May 2016
SRR939969_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939969_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1517216
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23010.1516593550292114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAT306.1118044E-103072.097110-111
CCGCTCC4450.01825.1362108-109
CCGCGCC3150.01718.9114108-109
CGTCGCC1850.01596.4336108-109
CCGCTCG1600.01538.2303108-109
CGTCGCT953.8198777E-111293.5144110-111
CCGCGCG1350.01276.1615108-109
CCGTTCG602.37942E-81230.5842108-109
CGTCGTC752.3861503E-81228.8387110-111
CGTCGAG250.0054092591228.8387110-111
CGCGGTT551.5537704E-51117.1262110-111
TTGCGCG551.5537704E-51117.1262110-111
CGTCGCG551.5537704E-51117.1262110-111
TTGTTCG2500.01082.9142108-109
CCGCACG807.515155E-8922.9382108-109
CTCCGCC3000.0921.6291110-111
CGGTGTT2050.0899.15027110-111
CGGGTCG753.9379665E-5819.2258110-111
CGGCGCG753.1081254E-5819.2258110-111
CCGCGCT757.666022E-5656.31165108-109
Produced by FastQC (version 0.11.5)