##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939969_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1517216 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.867217324362517 34.0 31.0 34.0 30.0 34.0 2 32.04696430831206 34.0 31.0 34.0 30.0 34.0 3 32.11834570687364 34.0 31.0 34.0 30.0 34.0 4 35.414805802206146 37.0 35.0 37.0 33.0 37.0 5 35.443700830995716 37.0 35.0 37.0 33.0 37.0 6 35.514882521671275 37.0 37.0 37.0 35.0 37.0 7 35.426835071604835 37.0 37.0 37.0 35.0 37.0 8 35.44045145846076 37.0 37.0 37.0 35.0 37.0 9 37.11234458376395 39.0 39.0 39.0 35.0 39.0 10-11 37.089140570624096 39.0 39.0 39.0 35.0 39.0 12-13 37.035360159660854 39.0 38.0 39.0 35.0 39.0 14-15 38.56085422247063 41.0 39.5 41.0 35.0 41.0 16-17 38.55997827600025 41.0 39.0 41.0 35.0 41.0 18-19 38.57696926475861 41.0 39.5 41.0 34.0 41.0 20-21 38.625664045198576 41.0 39.5 41.0 34.0 41.0 22-23 38.61750634056061 41.0 39.0 41.0 34.0 41.0 24-25 38.63949398108114 41.0 39.0 41.0 34.0 41.0 26-27 38.605656676438954 41.0 39.0 41.0 34.0 41.0 28-29 38.55251493524983 41.0 39.0 41.0 34.0 41.0 30-31 38.48094701084091 41.0 39.0 41.0 34.0 41.0 32-33 38.38566987165967 41.0 39.0 41.0 34.0 41.0 34-35 38.32082478697826 40.0 39.0 41.0 34.0 41.0 36-37 38.48992793379453 41.0 39.0 41.0 34.0 41.0 38-39 38.46344389987978 41.0 39.0 41.0 34.0 41.0 40-41 38.40954715742518 41.0 39.0 41.0 34.0 41.0 42-43 38.34172589796047 41.0 39.0 41.0 33.0 41.0 44-45 38.25473136323372 41.0 39.0 41.0 33.0 41.0 46-47 38.168787766540824 41.0 38.5 41.0 33.0 41.0 48-49 38.082184408811926 41.0 38.0 41.0 33.0 41.0 50-51 37.97045608535634 40.5 38.0 41.0 33.0 41.0 52-53 37.867903449475875 40.0 38.0 41.0 33.0 41.0 54-55 37.72971383112227 40.0 38.0 41.0 33.0 41.0 56-57 37.5587042319617 40.0 37.5 41.0 32.0 41.0 58-59 37.40210194197794 40.0 37.0 41.0 32.0 41.0 60-61 37.195104059013346 40.0 37.0 41.0 31.5 41.0 62-63 36.96340731972244 40.0 36.0 41.0 31.0 41.0 64-65 36.68787502900049 39.0 36.0 41.0 31.0 41.0 66-67 36.39817600130766 39.0 35.0 41.0 31.0 41.0 68-69 36.029044974479575 39.0 35.0 41.0 30.5 41.0 70-71 35.64224606120684 37.5 35.0 40.0 30.0 41.0 72-73 35.25433952713391 37.0 35.0 39.5 30.0 41.0 74-75 34.83568028547023 37.0 35.0 39.0 30.0 41.0 76-77 34.39510260898909 36.0 34.5 39.0 29.0 40.5 78-79 33.939732048699724 36.0 34.0 38.0 29.0 39.5 80-81 33.49842672368338 35.0 34.0 37.0 29.0 39.0 82-83 33.272148131841476 35.0 34.0 37.0 29.0 39.0 84-85 32.851746883766054 35.0 34.0 36.5 29.0 38.0 86-87 32.22599946217282 35.0 34.0 36.0 27.5 37.0 88-89 16.648942536856982 18.0 17.0 18.5 2.0 19.0 90-91 2.8029608177082235 2.0 2.0 2.0 2.0 2.0 92-93 2.3105553197435302 2.0 2.0 2.0 2.0 2.0 94-95 2.106055103558096 2.0 2.0 2.0 2.0 2.0 96-97 2.000059319174066 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0582060963236799 1101 2 0.1287717772346042 1101 3 0.1745316724673387 1101 4 0.11387177054441366 1101 5 0.16627238286272927 1101 6 0.20192018514411814 1101 7 0.2556616571608643 1101 8 0.22999535420329664 1101 9 0.21314219370696463 1101 10-11 0.2759466269298798 1101 12-13 0.290632409270863 1101 14-15 0.3366203941773307 1101 16-17 0.2829401997452763 1101 18-19 0.2333072493876287 1101 20-21 0.2139215776429424 1101 22-23 0.21665168455157868 1101 24-25 0.11676857806797614 1101 26-27 0.19332964999264135 1101 28-29 0.26479352258585465 1101 30-31 0.22107470611904745 1101 32-33 0.29302420633239024 1101 34-35 0.3154052705231578 1101 36-37 0.3499461465071434 1101 38-39 0.2589781690509625 1101 40-41 0.20188837999216247 1101 42-43 0.19464300717963567 1101 44-45 0.21906645008180448 1101 46-47 0.13940407885387174 1101 48-49 0.2115863211548401 1101 50-51 0.12917718069586925 1101 52-53 0.01679891185665383 1101 54-55 0.09682261427229832 1101 56-57 0.0386443442751343 1101 58-59 -0.034382254787075794 1101 60-61 -0.020588837727849807 1101 62-63 -0.0287774334912001 1101 64-65 0.054553565593757014 1101 66-67 0.03156440359136781 1101 68-69 0.08379730935076424 1101 70-71 0.10797933464360199 1101 72-73 -0.07243698215512495 1101 74-75 0.0618842892088054 1101 76-77 0.08719020072091865 1101 78-79 0.13573006972823265 1101 80-81 0.1454035803527134 1101 82-83 0.21275865281481998 1101 84-85 0.24958037471613892 1101 86-87 0.4846725849103706 1101 88-89 0.7402329033033617 1101 90-91 -0.08181971754516626 1101 92-93 -0.24931452406628285 1101 94-95 -0.040684006827143904 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.11820562053933159 1102 2 0.00526764932761381 1102 3 0.08921390415562058 1102 4 0.05024754951787713 1102 5 -0.008191466339702913 1102 6 -0.016619321782840757 1102 7 0.0027040723375648668 1102 8 0.0785275879859455 1102 9 0.011947355197868603 1102 10-11 0.03394310974749715 1102 12-13 -0.10637229620935074 1102 14-15 -0.016053858331815718 1102 16-17 0.053015657217542866 1102 18-19 0.003269364974400446 1102 20-21 0.04400943552386849 1102 22-23 0.028274782911054785 1102 24-25 0.06275904166988511 1102 26-27 0.040995467939147545 1102 28-29 0.09054851730067526 1102 30-31 0.1122249448201913 1102 32-33 0.0759688957162652 1102 34-35 0.0951310279594253 1102 36-37 0.12440822614259162 1102 38-39 0.14175074004587884 1102 40-41 0.07017402125524086 1102 42-43 0.1302542884155713 1102 44-45 0.16454709919481303 1102 46-47 0.1338712700739535 1102 48-49 0.0921444694620206 1102 50-51 0.09900191964553784 1102 52-53 0.01446700336074258 1102 54-55 0.11684358476107093 1102 56-57 0.09485049569317283 1102 58-59 0.1819441734270839 1102 60-61 0.11631740814922864 1102 62-63 0.10931207031101309 1102 64-65 0.06036233722530682 1102 66-67 0.0660753277555699 1102 68-69 0.0737829041795024 1102 70-71 -0.06295232445536669 1102 72-73 0.018884434152525387 1102 74-75 0.11003599889050264 1102 76-77 0.006431643352556193 1102 78-79 0.06742041611312999 1102 80-81 0.07320278333077823 1102 82-83 0.09489283571949869 1102 84-85 -0.03460009961293764 1102 86-87 -0.05733861532867479 1102 88-89 0.852862706081762 1102 90-91 0.19211417994404245 1102 92-93 0.05920970410571602 1102 94-95 0.14268535623401846 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.17155096209591747 1103 2 -0.0678991955447863 1103 3 -0.08131476845299801 1103 4 -0.049482199334363486 1103 5 -0.10611204675479513 1103 6 -0.1444705309038028 1103 7 -0.19164664051844227 1103 8 -0.14565985667604764 1103 9 -0.09253542827654115 1103 10-11 -0.09589844915263512 1103 12-13 -0.08845286636191219 1103 14-15 -0.14213656563071453 1103 16-17 -0.03476769486210429 1103 18-19 -0.035743143811906464 1103 20-21 -0.08571838910317808 1103 22-23 -0.07306939749795305 1103 24-25 -0.06592864335495108 1103 26-27 0.008567313945583521 1103 28-29 -0.0660080321072769 1103 30-31 -0.017465519420625242 1103 32-33 0.012684699556380963 1103 34-35 0.05029327452871257 1103 36-37 -0.045863221860017234 1103 38-39 -0.043614303989784275 1103 40-41 -0.06521538968718232 1103 42-43 -0.033843107796947436 1103 44-45 -0.06763844372434136 1103 46-47 -0.11062237105605988 1103 48-49 0.009221935065674813 1103 50-51 -0.10948490536303979 1103 52-53 -0.0929814674092242 1103 54-55 -0.10423567414110835 1103 56-57 -0.0985655462615469 1103 58-59 -0.020155348029348374 1103 60-61 -0.0980604554582527 1103 62-63 -0.1372691237269308 1103 64-65 -0.14342170783991293 1103 66-67 -0.17022448636824805 1103 68-69 -0.21124576413102147 1103 70-71 -0.2112532203772588 1103 72-73 -0.18482753512334682 1103 74-75 -0.16747562746331113 1103 76-77 -0.25329842289765736 1103 78-79 -0.26815983035961466 1103 80-81 -0.2741797083358719 1103 82-83 -0.19851150218502056 1103 84-85 -0.18051290642271312 1103 86-87 -0.16135158368079772 1103 88-89 0.8195716263533761 1103 90-91 -0.5798188464390028 1103 92-93 -0.3472600567944246 1103 94-95 -0.12501272159064136 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.07907691052454524 1104 2 0.07066140934163201 1104 3 0.13595068823418188 1104 4 0.1613116495060325 1104 5 0.20282278897835226 1104 6 0.14134127130433427 1104 7 0.24114899590907157 1104 8 0.2750463177325244 1104 9 0.31574611152827003 1104 10-11 0.30109991567012884 1104 12-13 0.35987114155307864 1104 14-15 0.2787091025703887 1104 16-17 0.2596681819080473 1104 18-19 0.2317843157994801 1104 20-21 0.19506905335563118 1104 22-23 0.15396256207984038 1104 24-25 0.15552873095856512 1104 26-27 0.27212404400443546 1104 28-29 0.27139290146784134 1104 30-31 0.22600184419835756 1104 32-33 0.12949299117074276 1104 34-35 0.203255644786573 1104 36-37 0.16649734892791201 1104 38-39 0.21606727559659333 1104 40-41 0.2147586618841828 1104 42-43 0.15877941425082298 1104 44-45 0.19707847428947645 1104 46-47 0.20338719718481713 1104 48-49 0.2862598360457511 1104 50-51 0.21614365624930088 1104 52-53 0.22353963490172646 1104 54-55 0.2176917813371233 1104 56-57 0.27828242358712174 1104 58-59 0.2460963246045509 1104 60-61 0.18675906798962671 1104 62-63 0.1967959062125786 1104 64-65 0.1376579611981512 1104 66-67 0.23820558849342177 1104 68-69 0.1908505821649058 1104 70-71 0.10999000513253065 1104 72-73 0.1818929270660874 1104 74-75 0.1577793361907993 1104 76-77 0.08852401183733605 1104 78-79 0.13066357824870067 1104 80-81 0.11361109747327447 1104 82-83 0.142673846316967 1104 84-85 0.16149907196091817 1104 86-87 0.08059749333527577 1104 88-89 0.4262995540439256 1104 90-91 -0.6223715570100494 1104 92-93 -0.3472600567944246 1104 94-95 -0.12501272159064136 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 9.537917405921803E-5 1105 2 0.05717416559122768 1105 3 0.08053972492847805 1105 4 0.10552313977014904 1105 5 0.043610656082009314 1105 6 0.11837520228563392 1105 7 0.1510439255408471 1105 8 0.13552282952482386 1105 9 0.27174187015560847 1105 10-11 0.1592669679161247 1105 12-13 0.18165316070351167 1105 14-15 0.08816061596927227 1105 16-17 0.176824855643666 1105 18-19 0.19498960466265913 1105 20-21 0.21718363468074386 1105 22-23 0.041044047845545606 1105 24-25 0.12451369336457674 1105 26-27 0.214014025368094 1105 28-29 0.10873866973466306 1105 30-31 0.11279816191572678 1105 32-33 0.08860149962135466 1105 34-35 0.13383931550182382 1105 36-37 0.06031040721465075 1105 38-39 0.10332146877183845 1105 40-41 0.1925758008855354 1105 42-43 0.1453363514656587 1105 44-45 0.01784433123954443 1105 46-47 0.0733987645648071 1105 48-49 0.2143412575482202 1105 50-51 0.103397849424546 1105 52-53 0.08313252738935972 1105 54-55 0.03093884129470581 1105 56-57 0.05816080543919355 1105 58-59 0.15562174070890933 1105 60-61 0.03792142902040041 1105 62-63 -0.018590315752597064 1105 64-65 -0.045697060972663905 1105 66-67 0.09965408152086042 1105 68-69 0.09620290528038566 1105 70-71 0.061447173694972435 1105 72-73 0.12838977816819863 1105 74-75 -0.007818954407483147 1105 76-77 0.08568839715961474 1105 78-79 0.18648823604704745 1105 80-81 0.06836175617129925 1105 82-83 0.0634332769448136 1105 84-85 -0.02792079180104423 1105 86-87 0.020724252540986754 1105 88-89 1.0362418778020412 1105 90-91 -0.8242946339331261 1105 92-93 -0.3472600567944246 1105 94-95 -0.12501272159064136 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 0.029536549295347214 1106 2 0.0858989644585435 1106 3 0.08899206151684069 1106 4 0.08272769994352558 1106 5 0.03843445166776149 1106 6 0.14801054037306471 1106 7 0.01754741875521404 1106 8 0.15530022379071795 1106 9 0.177546286331129 1106 10-11 0.035966088371417015 1106 12-13 0.05392440732215675 1106 14-15 -0.013210439014031294 1106 16-17 0.08351870582558973 1106 18-19 0.05281945804775745 1106 20-21 0.03699154105559899 1106 22-23 0.08056868607202716 1106 24-25 0.012078682347649305 1106 26-27 -0.21076638995838692 1106 28-29 0.0330981867991369 1106 30-31 0.04705662721695347 1106 32-33 0.1471690706765756 1106 34-35 0.07925671029836678 1106 36-37 0.17084655200365972 1106 38-39 0.13247922602328543 1106 40-41 0.1926192173251735 1106 42-43 -0.23162447363965555 1106 44-45 0.19617192998480704 1106 46-47 0.03586615700962881 1106 48-49 0.07240990135130687 1106 50-51 4.3214479382669424E-4 1106 52-53 -0.0382583303144699 1106 54-55 0.0285382739865625 1106 56-57 -0.031521880687684245 1106 58-59 0.016573055201924092 1106 60-61 0.0940564734438567 1106 62-63 0.01787708151626788 1106 64-65 0.1114577873773328 1106 66-67 0.07296533277059325 1106 68-69 0.05835824206532436 1106 70-71 0.07361161587211029 1106 72-73 0.07570694370181741 1106 74-75 0.03951612040748387 1106 76-77 0.04477051332512616 1106 78-79 0.07641761197189822 1106 80-81 0.058321101499629435 1106 82-83 0.047749389629778705 1106 84-85 0.09117713518455872 1106 86-87 0.2176268513766999 1106 88-89 0.7986523464704476 1106 90-91 -0.24297202446038257 1106 92-93 -0.3217908878936204 1106 94-95 -0.12501272159064136 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.09680086414034506 1107 2 0.0026612128525513867 1107 3 0.017569614445342552 1107 4 -0.0049909931921661155 1107 5 -0.06340792753584168 1107 6 0.04373272706474296 1107 7 -0.04123875371592334 1107 8 -0.10991032687305591 1107 9 -0.017011732459401685 1107 10-11 0.004700438203265378 1107 12-13 -0.07528786283837974 1107 14-15 -0.07169661542145889 1107 16-17 -0.07931241519510479 1107 18-19 -0.08433515674716574 1107 20-21 -0.11691705981888134 1107 22-23 -0.09845501235037801 1107 24-25 -0.06291380557163961 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-0.12501272159064136 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15694.0 3 2797.0 4 1822.0 5 1533.0 6 1531.0 7 1698.0 8 1937.0 9 2293.0 10 2795.0 11 3337.0 12 4069.0 13 4803.0 14 5485.0 15 6197.0 16 6899.0 17 7993.0 18 10012.0 19 12702.0 20 13831.0 21 19476.0 22 22808.0 23 28668.0 24 43314.0 25 73313.0 26 141056.0 27 425854.0 28 630948.0 29 23429.0 30 922.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.48427119144538 20.271998186151478 14.431761858561998 23.81196876384114 2 13.510291003355526 20.447680231795566 43.38725506673811 22.654773698110795 3 18.715821861216234 25.79579466707286 29.28962224275191 26.198761228958993 4 11.588427465250346 17.511926131185465 39.96806949007464 30.93157691348955 5 12.485524660175736 39.05011435188727 35.65325658875263 12.81110439918437 6 29.63645255520638 38.21097325627992 19.262122202771394 12.890451985742308 7 27.30316579840972 32.50130502182946 22.77645371522578 17.419075464535045 8 23.880581275177697 38.079218779659584 21.145571889566153 16.894628055596566 9 25.275175057473685 17.807154683314703 21.872561322843946 35.04510893636766 10-11 23.628555016808875 27.427350762537284 29.50287848473953 19.44121573591431 12-13 25.125404902696474 24.89217699483682 29.055082585484765 20.927335516981938 14-15 20.943985563031237 28.098504102250438 26.293751186383478 24.663759148334847 16-17 20.637254175008447 30.10530825444689 28.543002381011227 20.714435189533436 18-19 21.16196193213858 28.8818550312717 29.408087039373964 20.54809599721575 20-21 21.937216497427467 28.42994446282648 28.51763994036312 21.115199099382934 22-23 21.30512800655151 29.25275547346029 28.512118480827443 20.92999803916076 24-25 21.53721025878978 28.629773216206527 29.02480596039061 20.80821056461308 26-27 20.507693037774448 29.301068536055514 28.969902769282683 21.22133565688735 28-29 20.619865746067013 29.39138854184964 28.282470433189573 21.706275278893777 30-31 21.263946596924892 28.616030940881192 28.5000290004851 21.619993461708813 32-33 20.62123730719392 29.273733553563876 28.9217689451212 21.183260194121004 34-35 21.13622657303019 29.38235517049331 28.828247809308504 20.653170447168 36-37 20.9697112702258 28.435596944268593 28.907810991787912 21.686880793717698 38-39 21.153052696517832 29.2635326809103 28.34952307384051 21.23389154873136 40-41 22.042675532027083 28.314261120367835 28.925182703056123 20.717880644548963 42-43 20.750769831058992 29.268047529158668 28.814717218906203 21.166465420876133 44-45 20.56627457752348 29.331416223612415 28.69745840156418 21.404850797299922 46-47 21.62206963280113 28.703988093982662 28.364484687743868 21.309457585472337 48-49 21.123764428593745 28.788706661490508 28.38803780352528 21.699491106390468 50-51 20.761157528380114 29.50556463468769 28.567958869389354 21.165318967542845 52-53 20.930342152996015 29.1609105097758 28.731637420116847 21.17710991711134 54-55 20.718032307136625 28.76667229110697 29.046894649839043 21.468400751917365 56-57 21.058867030139414 28.87133407504271 28.158548288444095 21.91125060637378 58-59 21.320563453061396 28.924424735831945 27.916130597093623 21.838881214013036 60-61 20.7853726825976 28.667144295868223 29.169182239048364 21.378300782485816 62-63 21.02429713369751 28.623445837639466 29.176168719549487 21.176088309113535 64-65 21.00702866302491 29.05436668213359 28.37601897159007 21.562585683251427 66-67 21.05155099867125 29.471446386012275 27.933728618733262 21.543273996583213 68-69 21.578618683935176 28.432833238587214 28.990764312843453 20.997783764634157 70-71 21.889994569000063 28.430032375087 28.445784911311243 21.234188144601692 72-73 21.385517948663868 28.959620779111212 28.409138843777022 21.245722428447895 74-75 20.87880038175184 29.53557700419716 28.78739744373906 20.79822517031194 76-77 21.210295699491702 28.46196586379263 29.013634182608143 21.314104254107523 78-79 21.190241973355743 28.56178682946522 28.706097530833684 21.541873666345353 80-81 21.542384208972223 28.975768776495897 28.409204753970428 21.07264226056145 82-83 21.169679872924643 28.98982342589358 29.078736628416635 20.761760072765142 84-85 21.097424493282432 28.882835403792207 29.04823703414675 20.971503068778606 86-87 20.63585489468604 29.551282410411538 28.328372994622008 21.484489700280417 88-89 30.195769420775648 18.29449926714054 34.59598825791754 16.913743054166275 90-91 30.809679667076516 15.05315789685397 16.87617407954175 37.26098835652776 92-93 22.613982367478457 17.543403108702943 35.34211699653748 24.500497527281127 94-95 15.298554878292508 6.851486495274106 27.515718674065187 50.3342399523682 96-97 26.380131298355796 7.103761232029232 15.068253289648162 51.44785417996681 98-99 57.46869917161553 14.107122593335097 18.635340621099218 9.788837613950145 100-101 30.689119548852194 7.0320087841324 12.579752738396024 49.699118928619384 102-103 22.94267510553267 2.0056124829264728 37.13660789063949 37.91510452090137 104-105 41.410216381158286 4.5266350591525795 40.82000246633065 13.243146093358488 106-107 5.257544061268282 1.6550696271289922 6.346438736207006 86.74094757539572 108-109 18.424771628865855 10.955142266178983 21.379074030584185 49.24101207437098 110-111 40.147210443282034 2.6570943797542346 24.996116783343574 32.19957839362016 112-113 27.182502332445242 2.7227433678173396 38.75225515508184 31.342499144655573 114-115 27.824304392390193 3.778905527361816 23.550411239719008 44.84637884052899 116-117 19.170301127976934 4.052383413884396 20.080210382733835 56.69710507540483 118-119 19.863443802954187 4.187832721001724 15.51733429556872 60.43138918047537 120-121 24.965972448605793 4.143967629296293 29.761420654471642 41.12863926762627 122-123 33.98217568899181 1.583444298005907 33.945785636696435 30.48859437630585 124-125 38.23006713693072 1.8615149958488657 19.620390476988074 40.28802739023234 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6.0 1 19.5 2 67.5 3 98.0 4 121.5 5 165.5 6 175.0 7 169.0 8 163.0 9 159.5 10 167.0 11 168.0 12 161.5 13 175.0 14 202.0 15 240.0 16 307.0 17 430.5 18 615.0 19 918.0 20 1427.5 21 2137.5 22 3130.5 23 4470.0 24 6282.0 25 8668.5 26 11378.5 27 14476.5 28 18199.0 29 22612.0 30 27882.5 31 33973.5 32 40548.0 33 47538.5 34 54116.5 35 59484.5 36 63121.0 37 64764.0 38 65368.0 39 65281.0 40 64666.5 41 63487.0 42 62300.0 43 61480.5 44 60468.0 45 58579.5 46 55825.5 47 52856.0 48 49938.0 49 47369.0 50 44786.5 51 41526.0 52 37661.0 53 33547.0 54 29297.5 55 25359.0 56 21824.5 57 18673.5 58 15645.5 59 12671.5 60 10066.0 61 7826.5 62 6109.5 63 4708.5 64 3378.5 65 2337.5 66 1692.0 67 1189.0 68 797.0 69 563.5 70 373.5 71 242.5 72 163.5 73 106.0 74 72.0 75 55.0 76 35.5 77 24.0 78 18.5 79 13.0 80 14.0 81 9.0 82 9.0 83 8.0 84 3.5 85 5.0 86 5.5 87 4.0 88 3.5 89 3.0 90 4.5 91 4.5 92 2.0 93 1.0 94 3.0 95 3.0 96 2.5 97 4.5 98 5.5 99 2.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 0.0011204732879168161 3 0.046993967899099404 4 0.052596334338683486 5 0.055628203235399576 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.579672241790227E-4 12-13 0.0020102608989095816 14-15 0.0 16-17 2.3068567692405037E-4 18-19 0.007217166178052433 20-21 0.0022409465758336322 22-23 5.602366439584081E-4 24-25 0.0 26-27 0.0 28-29 0.0010875181912133802 30-31 0.0 32-33 0.0011863834813236876 34-35 6.591019340687153E-5 36-37 9.88652901103073E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 9.88652901103073E-5 46-47 0.0 48-49 1.977305802206146E-4 50-51 3.2955096703435765E-5 52-53 0.0 54-55 0.001845485415392403 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.3068567692405037E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.3068567692405037E-4 80-81 0.0 82-83 3.954611604412292E-4 84-85 0.0 86-87 9.556978043996372E-4 88-89 19.282752093307742 90-91 26.266563231603147 92-93 40.35288976651973 94-95 44.7609635015713 96-97 48.4307771602725 98-99 93.03012886761014 100-101 51.64857871258938 102-103 53.096460886254825 104-105 74.87945025625883 106-107 81.64747142134013 108-109 12.57642286925527 110-111 39.10903259654525 112-113 52.03207717292726 114-115 86.81763176765865 116-117 66.46575042709806 118-119 71.87058401704174 120-121 80.38822422120515 122-123 75.82028531204521 124-125 50.02514473878472 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1517216.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.14095005470858 #Duplication Level Percentage of deduplicated Percentage of total 1 73.57835573825027 42.043371503469395 2 14.796536971548594 16.909763601478144 3 5.211230827187313 8.93324041259604 4 2.4074643666605584 5.502592045353664 5 1.2300852595046772 3.5144120188195003 6 0.7300623049010446 2.502987222070561 7 0.4590529762425942 1.8361506231560383 8 0.314502021157007 1.437675542642995 9 0.22342301209006982 1.1489942857420108 >10 0.9644426663252379 9.574921746407014 >50 0.054754383222507405 2.1797215032759856 >100 0.0269768929310896 2.9112461305138995 >500 0.002420895539203062 0.9566005908649773 >1k 6.916844397723035E-4 0.5483227736097005 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2301 0.1516593550292114 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.591019340687153E-5 0.0 10-11 0.0 0.0 0.0 6.591019340687153E-5 0.0 12-13 0.0 0.0 0.0 6.591019340687153E-5 0.0 14-15 3.2955096703435765E-5 0.0 0.0 1.3182038681374306E-4 0.0 16-17 6.591019340687153E-5 0.0 0.0 1.3182038681374306E-4 0.0 18-19 6.591019340687153E-5 0.0 0.0 1.3182038681374306E-4 0.0 20-21 6.591019340687153E-5 0.0 0.0 1.3182038681374306E-4 0.0 22-23 6.591019340687153E-5 0.0 0.0 1.9773058022061458E-4 0.0 24-25 6.591019340687153E-5 0.0 0.0 7.250121274755869E-4 0.0 26-27 6.591019340687153E-5 0.0 0.0 0.0010875181912133802 0.0 28-29 6.591019340687153E-5 0.0 0.0 0.0018125303186889673 0.0 30-31 6.591019340687153E-5 0.0 0.0 0.003625060637377934 0.0 32-33 6.591019340687153E-5 0.0 0.0 0.009095606690148271 0.0 34-35 6.591019340687153E-5 0.0 0.0 0.016378683061607578 0.0 36-37 6.591019340687153E-5 0.0 0.0 0.028539113745175374 0.0 38-39 6.591019340687153E-5 0.0 0.0 0.04992697150570519 0.0 40-41 6.591019340687153E-5 0.0 0.0 0.07523648577394386 0.0 42-43 6.591019340687153E-5 0.0 0.0 0.10202897939383714 0.0 44-45 6.591019340687153E-5 0.0 0.0 0.13290790500495644 0.0 46-47 6.591019340687153E-5 0.0 0.0 0.16741189125345368 0.0 48-49 6.591019340687153E-5 0.0 0.0 0.20540911775251514 0.0 50-51 6.591019340687153E-5 0.0 0.0 0.253194007972497 0.0 52-53 6.591019340687153E-5 0.0 0.0 0.29903454748697617 0.0 54-55 6.591019340687153E-5 0.0 0.0 0.3480058211882817 0.0 56-57 6.591019340687153E-5 0.0 0.0 0.4093023010566722 0.0 58-59 6.591019340687153E-5 0.0 0.0 0.4761681922679434 0.0 60-61 6.591019340687153E-5 0.0 0.0 0.551536498428701 0.0 62-63 6.591019340687153E-5 0.0 0.0 0.6296730327125472 0.0 64-65 6.591019340687153E-5 0.0 0.0 0.7075788813194694 0.0 66-67 6.591019340687153E-5 0.0 0.0 0.7953053487440154 0.0 68-69 6.591019340687153E-5 0.0 0.0 0.9005639276147892 0.0 70-71 6.591019340687153E-5 0.0 0.0 1.0233875730284943 0.0 72-73 6.591019340687153E-5 0.0 0.0 1.1453543859279103 0.0 74-75 6.591019340687153E-5 0.0 0.0 1.2640586442536856 0.0 76-77 6.591019340687153E-5 0.0 0.0 1.3945937822959946 0.0 78-79 6.591019340687153E-5 0.0 0.0 1.4314705355071393 0.0 80-81 6.591019340687153E-5 0.0 0.0 1.4324921435049458 0.0 82-83 6.591019340687153E-5 0.0 0.0 1.4324921435049458 0.0 84-85 6.591019340687153E-5 0.0 0.0 1.4324921435049458 0.0 86-87 6.591019340687153E-5 0.0 0.0 1.4324921435049458 0.0 88-89 6.591019340687153E-5 0.0 0.0 1.4324921435049458 0.0 90-91 6.591019340687153E-5 0.0 0.0 1.4324921435049458 0.0 92-93 6.591019340687153E-5 0.0 0.0 1.4324921435049458 0.0 94-95 6.591019340687153E-5 0.0 0.0 1.4324921435049458 0.0 96-97 6.591019340687153E-5 0.0 0.0 1.4324921435049458 0.0 98-99 6.591019340687153E-5 0.0 0.0 1.4324921435049458 0.0 100-101 6.591019340687153E-5 0.0 0.0 1.4324921435049458 0.0 102-103 6.591019340687153E-5 0.0 0.0 1.4324921435049458 0.0 104-105 6.591019340687153E-5 0.0 0.0 1.4324921435049458 0.0 106-107 6.591019340687153E-5 0.0 0.0 1.4324921435049458 0.0 108-109 6.591019340687153E-5 0.0 0.0 1.4324921435049458 0.0 110-111 6.591019340687153E-5 0.0 0.0 1.4324921435049458 0.0 112-113 6.591019340687153E-5 0.0 0.0 1.4324921435049458 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCGAT 30 6.1118044E-10 3072.097 110-111 CCGCTCC 445 0.0 1825.1362 108-109 CCGCGCC 315 0.0 1718.9114 108-109 CGTCGCC 185 0.0 1596.4336 108-109 CCGCTCG 160 0.0 1538.2303 108-109 CGTCGCT 95 3.8198777E-11 1293.5144 110-111 CCGCGCG 135 0.0 1276.1615 108-109 CCGTTCG 60 2.37942E-8 1230.5842 108-109 CGTCGTC 75 2.3861503E-8 1228.8387 110-111 CGTCGAG 25 0.005409259 1228.8387 110-111 CGCGGTT 55 1.5537704E-5 1117.1262 110-111 TTGCGCG 55 1.5537704E-5 1117.1262 110-111 CGTCGCG 55 1.5537704E-5 1117.1262 110-111 TTGTTCG 250 0.0 1082.9142 108-109 CCGCACG 80 7.515155E-8 922.9382 108-109 CTCCGCC 300 0.0 921.6291 110-111 CGGTGTT 205 0.0 899.15027 110-111 CGGGTCG 75 3.9379665E-5 819.2258 110-111 CGGCGCG 75 3.1081254E-5 819.2258 110-111 CCGCGCT 75 7.666022E-5 656.31165 108-109 >>END_MODULE