FastQCFastQC Report
Fri 27 May 2016
SRR939969_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939969_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1517216
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42100.2774819142429291No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29970.19753284964039397No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25570.16853236454137052No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19280.12707485288844833No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG54400.032.3120231
TACATGG54350.031.7872942
ACATGGG52550.030.3820553
GTATCAA92100.030.213311
CATGGGG27200.028.0347124
TAGGACC16350.027.3274154
GTCCTAC34550.027.0765111
ATCAACG102600.026.8836823
CAACGCA103150.026.7980885
TCAACGC103350.026.746234
GTAGGAC37050.026.6916223
AACGCAG105550.026.1542326
TATCAAC106000.026.0213722
GTCTACG1159.965692E-425.9067671
CCTACAG37400.025.8046823
GACGTGG21100.025.6590847
TCCTACA38200.025.4202212
AGTGGAC23200.024.618728
CTACAGT39450.024.6147634
TACAGTG42200.024.5635935
Produced by FastQC (version 0.11.5)