##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939969_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1517216 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.85031004154978 34.0 31.0 34.0 31.0 34.0 2 32.928505895007696 34.0 31.0 34.0 31.0 34.0 3 32.97400699702613 34.0 33.0 34.0 31.0 34.0 4 36.37892495201738 37.0 37.0 37.0 35.0 37.0 5 36.27694013245313 37.0 37.0 37.0 35.0 37.0 6 36.40624340898066 37.0 37.0 37.0 35.0 37.0 7 36.40512425389661 37.0 37.0 37.0 35.0 37.0 8 36.42591430620294 37.0 37.0 37.0 35.0 37.0 9 38.264839680045554 39.0 39.0 39.0 37.0 39.0 10-11 38.264327887393755 39.0 39.0 39.0 37.0 39.0 12-13 38.25656531436526 39.0 39.0 39.0 37.0 39.0 14-15 39.81281208476578 41.0 40.0 41.0 37.5 41.0 16-17 39.83122607459979 41.0 40.0 41.0 38.0 41.0 18-19 39.86766716143252 41.0 40.0 41.0 38.0 41.0 20-21 39.87877039261384 41.0 40.0 41.0 38.0 41.0 22-23 39.83150586337081 41.0 40.0 41.0 38.0 41.0 24-25 39.79826735283572 41.0 40.0 41.0 37.0 41.0 26-27 39.71120427150781 41.0 40.0 41.0 37.0 41.0 28-29 39.63703256490836 41.0 40.0 41.0 37.0 41.0 30-31 39.518445626726844 41.0 40.0 41.0 36.5 41.0 32-33 39.37639235283572 41.0 39.0 41.0 36.0 41.0 34-35 39.284912299896654 41.0 39.0 41.0 35.0 41.0 36-37 39.26706447862401 41.0 39.0 41.0 35.5 41.0 38-39 39.32729782707274 41.0 40.0 41.0 35.0 41.0 40-41 39.26182494779913 41.0 40.0 41.0 35.0 41.0 42-43 39.16247192225761 41.0 40.0 41.0 35.0 41.0 44-45 39.02538695874549 41.0 39.0 41.0 35.0 41.0 46-47 38.841068114230275 41.0 39.0 41.0 35.0 41.0 48-49 38.63101694155611 41.0 39.0 41.0 35.0 41.0 50-51 38.48372512549301 41.0 39.0 41.0 35.0 41.0 52-53 38.37529231170776 41.0 39.0 41.0 35.0 41.0 54-55 38.26741709815873 41.0 39.0 41.0 35.0 41.0 56-57 38.13042671577415 41.0 38.5 41.0 34.5 41.0 58-59 37.97706127538861 40.5 38.0 41.0 34.0 41.0 60-61 37.80023773806762 40.0 37.5 41.0 34.0 41.0 62-63 37.59141117678696 40.0 37.0 41.0 34.0 41.0 64-65 37.35795529443402 40.0 36.5 41.0 34.0 41.0 66-67 37.09583111435683 39.0 36.0 41.0 33.0 41.0 68-69 36.77197577668572 39.0 35.5 41.0 33.0 41.0 70-71 36.411245333558306 38.5 35.0 41.0 32.5 41.0 72-73 36.06334595733237 37.0 35.0 40.0 32.0 41.0 74-75 35.693959858055806 37.0 35.0 39.0 32.0 41.0 76-77 35.30476510925274 36.5 35.0 39.0 32.0 41.0 78-79 34.92579533830384 36.0 35.0 38.5 31.5 40.0 80-81 34.62131232467888 36.0 35.0 37.0 31.5 39.0 82-83 34.363288088182564 35.0 35.0 37.0 32.0 39.0 84-85 34.07543322770127 35.0 35.0 36.5 31.0 38.5 86-87 33.84378855746314 35.0 35.0 36.0 31.0 37.0 88-89 33.62337795014025 35.0 35.0 36.0 31.0 37.0 90-91 33.44729557294413 35.0 35.0 36.0 31.0 36.5 92-93 33.3077775346424 35.0 35.0 35.0 31.0 36.0 94-95 33.20401215120326 35.0 35.0 35.0 31.0 36.0 96-97 33.109030289688484 35.0 35.0 35.0 31.0 36.0 98-99 33.02280163140911 35.0 34.0 35.0 31.0 36.0 100-101 32.98593608293084 35.0 34.0 35.0 31.0 36.0 102-103 32.16593154830954 34.5 33.0 35.0 28.0 35.0 104-105 32.82867963427752 35.0 34.0 35.0 30.5 35.0 106-107 32.89432948242043 35.0 34.0 35.0 31.0 35.0 108-109 32.898745465378695 35.0 34.0 35.0 31.0 35.0 110-111 32.87260778953029 35.0 34.0 35.0 31.0 35.0 112-113 32.807120410014136 35.0 34.0 35.0 31.0 35.0 114-115 32.76081388543227 35.0 34.0 35.0 30.5 35.0 116-117 32.71405917153523 35.0 34.0 35.0 30.0 35.0 118-119 32.64607939805539 35.0 34.0 35.0 30.0 35.0 120-121 32.561222330900804 35.0 34.0 35.0 30.0 35.0 122-123 32.416129278889755 35.0 34.0 35.0 30.0 35.0 124-125 30.459445128445783 33.5 30.5 34.5 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.035285482798038004 1101 2 0.08025552506756384 1101 3 0.026673809070203447 1101 4 -0.0011166984614590092 1101 5 -0.031177044938559106 1101 6 -0.03595732120187023 1101 7 0.01052633083413923 1101 8 0.02441896754341144 1101 9 -0.0024683508094085482 1101 10-11 0.03549830805209808 1101 12-13 0.01098383192028507 1101 14-15 0.09810913511869046 1101 16-17 0.15643086716988108 1101 18-19 0.10811928072920551 1101 20-21 0.09333361124852502 1101 22-23 0.06745995117003645 1101 24-25 0.12144999531624023 1101 26-27 0.06276531258576767 1101 28-29 0.11632513494164698 1101 30-31 0.11245449270744245 1101 32-33 0.09531031288674541 1101 34-35 0.11799412813157772 1101 36-37 0.06257105549117625 1101 38-39 0.014729069842388753 1101 40-41 -0.006393124910822223 1101 42-43 -0.017826030773001378 1101 44-45 0.03364344719866352 1101 46-47 0.03962567194821531 1101 48-49 0.13561765627242295 1101 50-51 0.1730666139599819 1101 52-53 0.12441684013058563 1101 54-55 0.1541188954178878 1101 56-57 0.16279353138353514 1101 58-59 0.1425166560491533 1101 60-61 0.13571986013725024 1101 62-63 0.19665556708379484 1101 64-65 0.22750568662365112 1101 66-67 0.032635030377193175 1101 68-69 0.06587994798100283 1101 70-71 0.06938448948545783 1101 72-73 0.02331757489111652 1101 74-75 0.047688260973025365 1101 76-77 0.06949864630896485 1101 78-79 0.1422239538690988 1101 80-81 0.07576397558262471 1101 82-83 0.1249947515951888 1101 84-85 0.17817344520803147 1101 86-87 0.14585019532589882 1101 88-89 0.17155995342635322 1101 90-91 0.2124264655196555 1101 92-93 0.2334074644362829 1101 94-95 0.2618832264570301 1101 96-97 0.23135757262216572 1101 98-99 0.19167088336287463 1101 100-101 0.21081928539421568 1101 102-103 0.23853699143709406 1101 104-105 0.2176815196812001 1101 106-107 0.18451792235508435 1101 108-109 0.21037650475761183 1101 110-111 0.2115087298886067 1101 112-113 0.22377056684369734 1101 114-115 0.28661691759265295 1101 116-117 0.26647363609718866 1101 118-119 0.2948161791298176 1101 120-121 0.2563148830422364 1101 122-123 0.3407053835972178 1101 124-125 0.5124912989171868 1102 1 0.058930033685292926 1102 2 0.14318827099425846 1102 3 0.10992723189434628 1102 4 0.0583241766095739 1102 5 0.0101354003364591 1102 6 0.024528718370575575 1102 7 -0.011909762598101281 1102 8 -0.009940871439432897 1102 9 0.020518100355360502 1102 10-11 0.1232494549666967 1102 12-13 0.09151458553854752 1102 14-15 0.06916775504907946 1102 16-17 0.16318400518334641 1102 18-19 0.11821544437518128 1102 20-21 0.08488885679385305 1102 22-23 0.16082622532883306 1102 24-25 0.16214376413329035 1102 26-27 0.07240519674408574 1102 28-29 0.10021860342510536 1102 30-31 0.15223088663903184 1102 32-33 0.11085618286299592 1102 34-35 0.0905040169870972 1102 36-37 0.1005805703856737 1102 38-39 0.04416984859522444 1102 40-41 0.0061956642327345435 1102 42-43 -0.0047367229671237965 1102 44-45 -0.013092720891386023 1102 46-47 -0.05122266518520746 1102 48-49 -0.05479256743443983 1102 50-51 -0.11066452475188981 1102 52-53 -0.089641294400856 1102 54-55 -0.1391029462354325 1102 56-57 -0.09909588680747561 1102 58-59 0.002181370144242578 1102 60-61 -0.1946719600729523 1102 62-63 -0.1188917140661303 1102 64-65 -0.10180557774540233 1102 66-67 -0.08950574696446267 1102 68-69 -0.12039766482385517 1102 70-71 -0.12429764118071773 1102 72-73 -0.08024330907704069 1102 74-75 -0.05173430019966929 1102 76-77 4.8049391161697486E-4 1102 78-79 0.04781710712322962 1102 80-81 -0.016143861784762237 1102 82-83 -0.020307529645450018 1102 84-85 -0.0012581833154996502 1102 86-87 -0.03976046992152504 1102 88-89 -0.10532732896450625 1102 90-91 -0.10257247386013546 1102 92-93 -0.10391545846727013 1102 94-95 -0.052065508515369174 1102 96-97 -0.04827410656923092 1102 98-99 -0.04307281725864698 1102 100-101 -0.04574855207621198 1102 102-103 -0.13342766919019056 1102 104-105 -0.06101609693606491 1102 106-107 -0.07127914549482028 1102 108-109 -0.08369394506407701 1102 110-111 -0.08670642660943173 1102 112-113 -0.13318323782611685 1102 114-115 -0.1461324810345701 1102 116-117 -0.15756949295420242 1102 118-119 -0.21273259204030381 1102 120-121 -0.2589257926277213 1102 122-123 -0.2286902017587451 1102 124-125 -0.10551776350574471 1103 1 -0.018419851952252486 1103 2 0.01554006432585453 1103 3 0.07116963335384696 1103 4 0.03511709493183446 1103 5 -0.07602823022023841 1103 6 8.382660321757385E-4 1103 7 -0.04369452445374833 1103 8 -0.004516672356842832 1103 9 0.07965463239899861 1103 10-11 0.08322854637533084 1103 12-13 0.07231729823320165 1103 14-15 0.04064690233297341 1103 16-17 0.10185577402239687 1103 18-19 0.09704962840119435 1103 20-21 0.0970544565404694 1103 22-23 0.06962390197567458 1103 24-25 0.08918851790851079 1103 26-27 0.08828369151066084 1103 28-29 0.1277370075481059 1103 30-31 -0.006872450866758584 1103 32-33 -0.013970157465656996 1103 34-35 0.04011874213698263 1103 36-37 0.11691433286750907 1103 38-39 -0.004381130478328998 1103 40-41 0.02669296072938465 1103 42-43 -0.05414170095756532 1103 44-45 -0.1773861764758422 1103 46-47 -0.20037789064686962 1103 48-49 -0.07550047071004684 1103 50-51 -0.11099250265569793 1103 52-53 -0.09400022654183005 1103 54-55 -0.0717395248969055 1103 56-57 -0.029904451527237086 1103 58-59 -0.10365630592070829 1103 60-61 -0.036384930474930854 1103 62-63 -0.15704824019822894 1103 64-65 -0.20004851547522406 1103 66-67 -0.03025746438504484 1103 68-69 -0.047815691838394514 1103 70-71 -0.11083666096320144 1103 72-73 -0.16472241602494364 1103 74-75 -0.14940063161473205 1103 76-77 -0.10916066784430711 1103 78-79 -0.14316125228633325 1103 80-81 -0.1808925850844858 1103 82-83 -0.1051840453034174 1103 84-85 -0.1287228942816725 1103 86-87 -0.12283641333740292 1103 88-89 -0.1682898290554249 1103 90-91 -0.11687951804523777 1103 92-93 -0.10099936460474623 1103 94-95 -0.08007951634584742 1103 96-97 -0.11863594686911938 1103 98-99 -0.11156234014998034 1103 100-101 -0.10704405157135 1103 102-103 -0.11125874337734132 1103 104-105 -0.1282247867635249 1103 106-107 -0.12420228162482516 1103 108-109 -0.1523947359801454 1103 110-111 -0.09552182464302206 1103 112-113 -0.011114292374259094 1103 114-115 -0.004519094246120403 1103 116-117 0.029491315868405366 1103 118-119 0.009220833774854498 1103 120-121 -0.029360766710190944 1103 122-123 0.021738450814133614 1103 124-125 0.011195421801108552 1104 1 0.034867422597365305 1104 2 0.04328705875126815 1104 3 0.0972065372116262 1104 4 0.02424562037911926 1104 5 -0.030022402479573884 1104 6 0.011621911159970466 1104 7 0.05020223464047291 1104 8 -0.025768164165455687 1104 9 0.005335439603079806 1104 10-11 0.05035935280444903 1104 12-13 0.05285952868727861 1104 14-15 0.020469383565895782 1104 16-17 0.07574524844078923 1104 18-19 0.061057646711049074 1104 20-21 0.14352074295739214 1104 22-23 0.06737036684132391 1104 24-25 0.14062104166120548 1104 26-27 0.17447697049307465 1104 28-29 0.21788390226129195 1104 30-31 0.1743274024064334 1104 32-33 0.2084055509819862 1104 34-35 0.1535491528170425 1104 36-37 0.2076976680090894 1104 38-39 0.21496995214849335 1104 40-41 0.23440955067446367 1104 42-43 0.3197675350814322 1104 44-45 0.29097940833896985 1104 46-47 0.3641399258102922 1104 48-49 0.309510792122083 1104 50-51 0.3416842282297736 1104 52-53 0.30770637668099 1104 54-55 0.29329312698342136 1104 56-57 0.34438096568835874 1104 58-59 0.3103280113605038 1104 60-61 0.37641304376521134 1104 62-63 0.37920852137587957 1104 64-65 0.3436766533381004 1104 66-67 0.33509163174683465 1104 68-69 0.38578558582142364 1104 70-71 0.4139954944225437 1104 72-73 0.4296422284983734 1104 74-75 0.4132420912007717 1104 76-77 0.3854426660573296 1104 78-79 0.34282317349005353 1104 80-81 0.2654836761718897 1104 82-83 0.3303220330094305 1104 84-85 0.3166210483295515 1104 86-87 0.22980066514593744 1104 88-89 0.22998884337698655 1104 90-91 0.21515381063830574 1104 92-93 0.2100856775275446 1104 94-95 0.22207792306477359 1104 96-97 0.18414663138948129 1104 98-99 0.2746956484528553 1104 100-101 0.3167477771922691 1104 102-103 0.24303611550143955 1104 104-105 0.3006366080710663 1104 106-107 0.31268323530300535 1104 108-109 0.3236210500673806 1104 110-111 0.2931598686049668 1104 112-113 0.2186045372211538 1104 114-115 0.25219662773757534 1104 116-117 0.19283531789364616 1104 118-119 0.22703070373564316 1104 120-121 0.13202160385765183 1104 122-123 0.3469165637214431 1104 124-125 0.3042276422839656 1105 1 0.02107492854171511 1105 2 0.08813475491475486 1105 3 -0.01352702746930845 1105 4 0.06076319637038097 1105 5 -0.04427277199309998 1105 6 0.04335187710046284 1105 7 -0.0676484818944516 1105 8 0.020172006774714646 1105 9 0.06606418454234841 1105 10-11 0.0883268677456428 1105 12-13 0.0975827148578361 1105 14-15 0.08961661060786241 1105 16-17 0.12502710693317454 1105 18-19 0.044124287672431706 1105 20-21 0.06548109254931944 1105 22-23 0.14904490575270302 1105 24-25 0.12239438527664959 1105 26-27 0.09215176645734857 1105 28-29 0.09910538545645409 1105 30-31 0.09203834643317066 1105 32-33 0.13788469756639898 1105 34-35 0.16587162566582947 1105 36-37 0.2577426522646036 1105 38-39 0.25515454825413997 1105 40-41 0.2318068901770758 1105 42-43 0.16857223914929875 1105 44-45 0.07407898420170511 1105 46-47 0.16371096880239122 1105 48-49 0.2187952211433597 1105 50-51 0.11401569792966626 1105 52-53 0.13261319500885804 1105 54-55 0.07469374391035188 1105 56-57 0.2159950971709108 1105 58-59 0.2078723529837987 1105 60-61 0.17143344733824506 1105 62-63 0.08281047497782623 1105 64-65 0.13635948220512972 1105 66-67 0.1177293157003163 1105 68-69 0.14130820647562103 1105 70-71 0.20994771471687557 1105 72-73 0.14953892536875202 1105 74-75 0.17725549008259378 1105 76-77 0.2477145316186693 1105 78-79 0.26246603063291474 1105 80-81 0.15920837252816966 1105 82-83 0.2545396282796517 1105 84-85 0.0809557633221587 1105 86-87 0.0907109536877968 1105 88-89 0.17934139141374317 1105 90-91 0.2053295705509086 1105 92-93 0.054187117023715814 1105 94-95 0.11045672262778083 1105 96-97 0.13386467650227019 1105 98-99 0.11534293975804388 1105 100-101 0.16888613590958101 1105 102-103 0.1610844414445083 1105 104-105 0.06057330879724532 1105 106-107 0.07582506423430857 1105 108-109 0.14972878735932937 1105 110-111 0.14507732249609973 1105 112-113 0.016340061930364413 1105 114-115 0.11627187997072497 1105 116-117 0.10820868721439325 1105 118-119 0.21757597717565602 1105 120-121 0.06236428748980671 1105 122-123 0.06981354977632037 1105 124-125 0.1559603394113971 1106 1 0.03219014009434318 1106 2 0.0825919227880405 1106 3 0.010353823348189906 1106 4 0.04707556076937891 1106 5 -0.02796748688578532 1106 6 0.06845984534374594 1106 7 0.05024765392182218 1106 8 0.023391456374859843 1106 9 0.012828393476731037 1106 10-11 0.11740864622773017 1106 12-13 0.05244891383296846 1106 14-15 -0.06795581947056917 1106 16-17 0.015525419119079231 1106 18-19 -0.04695676961435424 1106 20-21 0.08228204867580047 1106 22-23 0.15983358949891624 1106 24-25 0.07750419871862846 1106 26-27 0.06255833729428417 1106 28-29 0.1324778538860727 1106 30-31 0.09301461331884298 1106 32-33 0.10821920917366157 1106 34-35 0.14399637315702307 1106 36-37 0.16973451409420193 1106 38-39 0.15741328852614345 1106 40-41 0.2327548157757704 1106 42-43 0.20883646436483616 1106 44-45 0.12236891918558257 1106 46-47 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-0.015490926361145796 2216 9 -0.0366436402821364 2216 10-11 -0.07339380438733656 2216 12-13 -0.0855982079202704 2216 14-15 -0.054485985974316975 2216 16-17 -0.0918064237137699 2216 18-19 -0.12615622245228053 2216 20-21 -0.1264323785300263 2216 22-23 -0.15121127714645866 2216 24-25 -0.13454756229801035 2216 26-27 -0.09773660113384608 2216 28-29 -0.2389906762288092 2216 30-31 -0.22644222507799583 2216 32-33 -0.2822494919293703 2216 34-35 -0.1381687562293905 2216 36-37 -0.16314376492620397 2216 38-39 -0.10257064504011737 2216 40-41 -0.13500569112344607 2216 42-43 -0.07304266314392294 2216 44-45 -0.007524810065966392 2216 46-47 -0.10337273601643915 2216 48-49 0.005593560136261999 2216 50-51 -0.013168921027705949 2216 52-53 0.01919822418366124 2216 54-55 -0.0596885183267446 2216 56-57 -0.1700589716306382 2216 58-59 -0.16410253703213584 2216 60-61 -0.055979909250481796 2216 62-63 -0.11604940962212851 2216 64-65 -0.06559871329286437 2216 66-67 -0.011252968298123278 2216 68-69 0.01475620906099806 2216 70-71 -0.06913931601370393 2216 72-73 -0.007008800977565954 2216 74-75 0.023326756835977847 2216 76-77 -0.10056039526160987 2216 78-79 -0.050956166904811084 2216 80-81 -0.06921964697908578 2216 82-83 0.0371296658098359 2216 84-85 0.04093853289072058 2216 86-87 -0.010096651736944295 2216 88-89 0.04490302065578078 2216 90-91 0.03600152489757136 2216 92-93 0.05017523598352369 2216 94-95 0.12823841419817938 2216 96-97 0.0531210234234436 2216 98-99 0.07707676613181036 2216 100-101 0.12442057972067033 2216 102-103 0.08325973063480063 2216 104-105 0.052069741724416474 2216 106-107 0.06512303377168394 2216 108-109 0.043961464767001246 2216 110-111 0.03937504031652139 2216 112-113 0.06082445629125033 2216 114-115 0.007276896446008152 2216 116-117 -0.16521105811300885 2216 118-119 0.0029729023312725644 2216 120-121 -0.04754938171514311 2216 122-123 -0.12429976507612395 2216 124-125 -0.2422553121863551 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 123.0 3 9.0 4 21.0 5 46.0 6 98.0 7 245.0 8 459.0 9 867.0 10 1899.0 11 3010.0 12 3752.0 13 5708.0 14 9366.0 15 10256.0 16 3960.0 17 2843.0 18 2504.0 19 2306.0 20 2154.0 21 2231.0 22 2305.0 23 2509.0 24 3003.0 25 3904.0 26 5035.0 27 6402.0 28 8295.0 29 10785.0 30 13731.0 31 18019.0 32 23355.0 33 31160.0 34 44459.0 35 72196.0 36 163690.0 37 554993.0 38 496615.0 39 4903.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.55723785867599 20.245054608930445 14.666624477246717 23.531083055146844 2 13.749723177187692 20.73818098411828 42.90793136903381 22.60416446966022 3 18.727392803661445 26.240232109337104 29.500084365047563 25.532290721953892 4 11.693127412313078 17.770706346360704 39.7204485056841 30.815717735642124 5 12.202365827435704 38.861649320829464 35.55578728805002 13.38019756368482 6 29.303935629468715 38.50882142028558 18.911150422879803 13.276092527365913 7 26.663705101976255 32.99404962773923 22.79062440680826 17.551620863476263 8 24.088659755763185 37.418996372302956 21.00722639360513 17.48511747832873 9 25.468160103769012 17.155830152068 22.09395366249763 35.28205608166537 10-11 23.570473815198365 27.213099519119226 29.39953836500538 19.81688830067703 12-13 25.480715995613018 24.71302701790648 29.334287273532578 20.471969712947928 14-15 21.040972412629447 27.496019024318226 27.30807610781853 24.154932455233798 16-17 20.083765292405726 29.74027091075724 29.324080857333712 20.851882939503323 18-19 20.547436884398792 27.889173327990214 31.373515702444475 20.189874085166515 20-21 21.289241185481256 27.328962875274527 30.72887692498332 20.652919014260892 22-23 20.68789264002726 28.16759144312913 30.753582142526415 20.390933774317194 24-25 20.92434432539599 27.6214131672748 31.27577088562209 20.17847162170713 26-27 19.943139276147893 28.312811096112885 31.20191851390969 20.54213111382954 28-29 19.979686478392004 28.364023316390018 30.523636713559572 21.132653491658406 30-31 20.668876415750955 27.580449982072423 30.774194313795793 20.976479288380823 32-33 20.094073619049627 28.17621222027714 31.13452534115116 20.595188819522072 34-35 20.602471895893533 28.289314112163332 31.015326756374833 20.092887235568305 36-37 20.434269082319194 27.513748866344674 31.020830257524306 21.031151793811823 38-39 20.65503527513551 28.25652379094341 30.364331776095167 20.72410915782591 40-41 21.57734956657457 27.459372956784005 30.743875624828632 20.219401851812794 42-43 20.37850905869698 28.31894074409972 30.625039546116046 20.677510651087257 44-45 20.285114314639443 28.379413346550525 30.354642977664355 20.980829361145677 46-47 21.411321789382658 27.889502878957252 29.787947134752073 20.91122819690802 48-49 21.03167907535908 28.19394206230359 29.468974753759518 21.305404108577815 50-51 20.73254566258199 28.96367425600574 29.40253727880539 20.90124280260688 52-53 20.903978075633265 28.65112811887035 29.60451906650075 20.840374738995635 54-55 20.62471658616835 28.325465853247 29.857614209183136 21.192203351401513 56-57 21.007951405732605 28.40554673823635 28.96726636154641 21.619235494484634 58-59 21.196125007909224 28.392068103684643 28.842201769556873 21.56960511884926 60-61 20.70222697361483 28.127603452639573 30.05755278088288 21.112616792862717 62-63 20.89883708054753 28.020763029127032 30.108830911353422 20.97156897897201 64-65 20.871945721637523 28.43665634952439 29.364902558370066 21.326495370468017 66-67 20.917324889798156 28.878781926897684 28.923798588994583 21.280094594309578 68-69 21.345115000105455 27.981085092696095 29.918844778858116 20.75495512834033 70-71 21.700206167084975 27.961279079577334 29.365891211271173 20.972623542066522 72-73 21.229903982030248 28.464865912302535 29.217758051589225 21.087472054077995 74-75 20.752252810410646 29.034066342564273 29.5771992913336 20.63648155569148 76-77 21.009599160567777 27.986621548942274 29.811806624765357 21.19197266572459 78-79 21.032404087486555 28.14951859194736 29.361870689473353 21.45620663109274 80-81 21.30032902368549 28.592863507898674 29.114312003037142 20.992495465378695 82-83 20.96847119988189 28.563994843186467 29.666145097336173 20.80138885959547 84-85 20.9657359268555 28.480783224010292 29.61259306519309 20.940887783941115 86-87 20.57693169594837 29.036373199333514 28.853241726952522 21.53345337776559 88-89 21.03909397211735 28.375788285913146 29.014029643768584 21.571088098200917 90-91 20.98433233302641 28.51088946026268 29.392051547553688 21.112726659157225 92-93 20.748430019193048 28.945351222238624 29.459747326682557 20.846471431885767 94-95 20.66100559351465 28.662206487132412 29.337527892599773 21.339260026753166 96-97 21.149526501170566 28.18530782696729 29.23225829413874 21.43290737772341 98-99 21.125598762187394 28.16821201001191 29.401674474399258 21.30451475340143 100-101 20.821571102315755 28.137903988557984 29.589675820500062 21.4508490886262 102-103 20.643726995214575 28.399699053164305 29.329463954102643 21.627109997518474 104-105 20.940520920220106 28.181913753834824 29.090539985189633 21.787025340755434 106-107 20.962882814948646 27.858569686055734 29.059643708508435 22.11890379048718 108-109 20.688361964275888 28.364818197606727 28.82213773237135 22.12468210574604 110-111 20.545192628071295 28.5511130035689 29.147039466203868 21.75665490215594 112-113 20.76910400833174 27.913933912951773 29.114686669874562 22.202275408841928 114-115 20.4639951276359 28.340555312406075 28.585656582700995 22.60979297725703 116-117 20.838412629676537 28.2626054705793 28.17761273566205 22.721369164082116 118-119 20.72312852579038 27.955303573064562 28.85698975687659 22.464578144268472 120-121 20.35547578904908 28.189514146398047 29.143306841654987 22.31170322289788 122-123 20.65079837964458 28.39098786051656 28.6983966496133 22.259817110225562 124-125 20.829445947402224 28.355018657804692 28.342034157146074 22.47350123764701 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 84.0 1 67.5 2 115.0 3 183.0 4 129.5 5 99.0 6 243.5 7 579.5 8 1007.5 9 1145.5 10 870.0 11 647.5 12 661.0 13 742.0 14 827.0 15 958.5 16 1137.5 17 1316.0 18 1559.0 19 1886.0 20 2255.5 21 2803.0 22 3600.0 23 4644.5 24 5865.5 25 7522.5 26 9667.0 27 12436.0 28 15588.5 29 18579.5 30 22253.0 31 27791.5 32 34811.5 33 42086.0 34 49551.0 35 57112.5 36 64025.5 37 67038.0 38 65380.5 39 62664.5 40 59829.0 41 58104.0 42 56810.0 43 55225.5 44 54671.5 45 54914.0 46 54034.0 47 54276.5 48 57197.5 49 53781.5 50 47185.0 51 43107.0 52 39198.0 53 36669.5 54 34064.5 55 29669.0 56 25621.5 57 22812.5 58 20127.0 59 17030.5 60 13377.0 61 10814.5 62 9005.5 63 6369.5 64 3963.0 65 2747.5 66 1987.5 67 1483.0 68 1074.0 69 742.0 70 494.0 71 303.0 72 214.0 73 145.5 74 98.5 75 74.5 76 41.5 77 19.0 78 10.5 79 4.5 80 3.5 81 2.0 82 1.5 83 1.0 84 0.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 1.0 98 6.5 99 7.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01634572796490414 2 0.0 3 0.0 4 0.0 5 0.1357090882247485 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.2955096703435765E-5 18-19 0.0 20-21 0.003954611604412292 22-23 1.6477548351717883E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 9.88652901103073E-4 92-93 0.0 94-95 8.238774175858941E-4 96-97 0.0 98-99 0.0015488895450614809 100-101 6.591019340687153E-5 102-103 2.965958703309219E-4 104-105 0.002339811865943939 106-107 0.007612627338493662 108-109 3.6250606373779343E-4 110-111 0.005437590956066902 112-113 0.008304684369265812 114-115 0.005800097019804695 116-117 0.001812530318688967 118-119 1.977305802206146E-4 120-121 6.591019340687153E-4 122-123 0.0011863834813236876 124-125 0.0014829793516546094 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1517216.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.29602678924278 #Duplication Level Percentage of deduplicated Percentage of total 1 72.3955068559282 40.03183886526215 2 15.324332940822183 16.94749449645958 3 5.460078388629267 9.057619225470283 4 2.5439982449896372 5.626919804269344 5 1.3176551435896122 3.6430547059457363 6 0.7696996365414773 2.5536799033120774 7 0.5081135900468274 1.9667663881045385 8 0.334741122819864 1.4807883275926725 9 0.2288880917782426 1.1390941849237528 >10 1.0225123517242063 9.837990313433867 >50 0.06027969957694707 2.2916023030439154 >100 0.030500642422071955 3.2751727312405525 >500 0.0028593221663013885 1.1237608199221303 >1k 8.339689651712383E-4 1.0242179310194255 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4210 0.2774819142429291 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2997 0.19753284964039397 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2557 0.16853236454137052 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1928 0.12707485288844833 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.591019340687153E-5 0.0 10-11 0.0 0.0 0.0 9.886529011030729E-5 0.0 12-13 0.0 0.0 0.0 1.3182038681374306E-4 0.0 14-15 0.0 0.0 0.0 1.977305802206146E-4 0.0 16-17 0.0 0.0 0.0 1.977305802206146E-4 0.0 18-19 0.0 0.0 0.0 1.977305802206146E-4 0.0 20-21 0.0 0.0 0.0 2.3068567692405035E-4 0.0 22-23 0.0 0.0 0.0 3.2955096703435766E-4 0.0 24-25 0.0 0.0 0.0 7.250121274755869E-4 0.0 26-27 0.0 0.0 0.0 0.0010875181912133802 0.0 28-29 0.0 0.0 0.0 0.00171366502857866 0.0 30-31 0.0 0.0 0.0 0.0035591504439710627 0.0 32-33 0.0 0.0 0.0 0.0090296964967414 0.0 34-35 0.0 0.0 0.0 0.016279817771497267 0.0 36-37 0.0 0.0 0.0 0.028407293358361632 0.0 38-39 0.0 0.0 0.0 0.04986106131229832 0.0 40-41 0.0 0.0 0.0 0.0753683061607576 0.0 42-43 0.0 0.0 0.0 0.10216079978065087 0.0 44-45 0.0 0.0 0.0 0.13310563558517707 0.0 46-47 0.0 0.0 0.0 0.16734598106004683 0.0 48-49 0.0 0.0 0.0 0.20531025246240484 0.0 50-51 0.0 0.0 0.0 0.2532599181659039 0.0 52-53 0.0 0.0 0.0 0.29933114335730704 0.0 54-55 0.0 0.0 0.0 0.34823650686520574 0.0 56-57 0.0 0.0 0.0 0.4095329867335963 0.0 58-59 0.0 0.0 0.0 0.4766625187184949 0.0 60-61 0.0 0.0 0.0 0.5519319595891423 0.0 62-63 0.0 0.0 0.0 0.6301014489696919 0.0 64-65 0.0 0.0 0.0 0.7075459262227659 0.0 66-67 0.0 0.0 0.0 0.7947121570033535 0.0 68-69 0.0 0.0 0.0 0.899871870584017 0.0 70-71 0.0 0.0 0.0 1.0224648303207982 0.0 72-73 0.0 0.0 0.0 1.144827104380655 0.0 74-75 0.0 0.0 0.0 1.263630227996541 0.0 76-77 0.0 0.0 0.0 1.3948574230696222 0.0 78-79 0.0 0.0 0.0 1.554623731887879 0.0 80-81 0.0 0.0 0.0 1.7252652226182694 0.0 82-83 0.0 0.0 0.0 1.908396694999262 0.0 84-85 0.0 0.0 0.0 2.0939997996330124 0.0 86-87 0.0 0.0 0.0 2.275582382468943 0.0 88-89 0.0 0.0 0.0 2.483496087570919 0.0 90-91 0.0 0.0 0.0 2.7161590702971763 0.0 92-93 0.0 0.0 0.0 2.9688917069158247 0.0 94-95 0.0 0.0 0.0 3.217504956446544 0.0 96-97 0.0 0.0 0.0 3.4696773564212346 0.0 98-99 0.0 0.0 0.0 3.7468297196971294 0.0 100-101 0.0 0.0 0.0 4.056212167548985 0.0 102-103 0.0 0.0 0.0 4.39034389302512 0.0 104-105 0.0 0.0 0.0 4.724706304178179 0.0 106-107 0.0 0.0 0.0 5.06282559635543 0.0 108-109 0.0 0.0 0.0 5.422003195326177 0.0 110-111 0.0 0.0 0.0 5.82115532659819 0.0 112-113 0.0 0.0 0.0 6.248648841035159 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 5440 0.0 32.312023 1 TACATGG 5435 0.0 31.787294 2 ACATGGG 5255 0.0 30.382055 3 GTATCAA 9210 0.0 30.21331 1 CATGGGG 2720 0.0 28.034712 4 TAGGACC 1635 0.0 27.327415 4 GTCCTAC 3455 0.0 27.076511 1 ATCAACG 10260 0.0 26.883682 3 CAACGCA 10315 0.0 26.798088 5 TCAACGC 10335 0.0 26.74623 4 GTAGGAC 3705 0.0 26.691622 3 AACGCAG 10555 0.0 26.154232 6 TATCAAC 10600 0.0 26.021372 2 GTCTACG 115 9.965692E-4 25.906767 1 CCTACAG 3740 0.0 25.804682 3 GACGTGG 2110 0.0 25.659084 7 TCCTACA 3820 0.0 25.420221 2 AGTGGAC 2320 0.0 24.61872 8 CTACAGT 3945 0.0 24.614763 4 TACAGTG 4220 0.0 24.563593 5 >>END_MODULE