FastQCFastQC Report
Fri 27 May 2016
SRR939968_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939968_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563599
Sequences flagged as poor quality0
Sequence length125
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34350.21968548201936686No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19660.1257355626346653No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15830.10124079127704737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCG1700.01143.833108-109
CCGCGCC7750.0972.25806108-109
CCGCTCC7000.0868.0876108-109
CGTCGCG901.2655437E-7810.215108-109
CTCCGCC4600.0803.16974110-111
CCTCGCC6850.0780.6452108-109
CGTCGCT1503.778805E-7615.7634110-111
CGTCGCC4000.0546.8952108-109
CGGCGCG901.3748775E-4540.1434108-109
TGCGTCG601.2139384E-5513.13617110-111
CGGCGCC3350.0507.896108-109
GGTCGCC1954.425601E-9498.59387108-109
CGTAGCT1251.809393E-4492.61075110-111
AAGCGCG1052.1822502E-4462.98108-109
CGTTGGT1402.5411448E-4439.83105110-111
TTTTTCG8400.0398.8400392-93
TTGCTCG1902.5055433E-6383.7861108-109
CGCCTCT3452.3355824E-8356.96432110-111
CCGCGCG4850.0350.81476108-109
AATCTCG1405.1671884E-4347.23505108-109
Produced by FastQC (version 0.11.5)