##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939968_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1563599 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.834527266901553 34.0 31.0 34.0 30.0 34.0 2 31.925862705207667 34.0 31.0 34.0 30.0 34.0 3 32.0550256171819 34.0 31.0 34.0 30.0 34.0 4 35.223494642808035 37.0 35.0 37.0 33.0 37.0 5 35.300985738670846 37.0 35.0 37.0 33.0 37.0 6 35.32320499053785 37.0 37.0 37.0 33.0 37.0 7 35.26607525330983 37.0 37.0 37.0 33.0 37.0 8 35.20907278656484 37.0 37.0 37.0 33.0 37.0 9 36.820508327262935 39.0 39.0 39.0 34.0 39.0 10-11 36.830837382218846 39.0 38.0 39.0 33.5 39.0 12-13 36.76076602760682 39.0 38.0 39.0 33.0 39.0 14-15 38.272318222255194 41.0 39.0 41.0 33.5 41.0 16-17 38.30441660553633 41.0 39.0 41.0 33.0 41.0 18-19 38.35535645648277 41.0 39.0 41.0 33.5 41.0 20-21 38.432893280182455 41.0 39.0 41.0 34.0 41.0 22-23 38.45727740936135 41.0 39.0 41.0 34.0 41.0 24-25 38.51889327122875 41.0 39.0 41.0 34.0 41.0 26-27 38.51392556531438 41.0 39.0 41.0 33.0 41.0 28-29 38.46679711358219 41.0 39.0 41.0 33.0 41.0 30-31 38.401641981096176 41.0 39.0 41.0 33.0 41.0 32-33 38.28276527421673 40.5 38.5 41.0 33.0 41.0 34-35 38.17887578592721 40.0 38.0 41.0 33.0 41.0 36-37 38.335746889068105 41.0 39.0 41.0 33.0 41.0 38-39 38.3090025000016 41.0 39.0 41.0 33.0 41.0 40-41 38.254247732315 41.0 39.0 41.0 33.0 41.0 42-43 38.144158444716325 41.0 38.5 41.0 33.0 41.0 44-45 38.03668491729657 41.0 38.0 41.0 33.0 41.0 46-47 37.931787817720526 41.0 38.0 41.0 33.0 41.0 48-49 37.812834045046074 40.0 38.0 41.0 32.0 41.0 50-51 37.67616377344831 40.0 38.0 41.0 32.0 41.0 52-53 37.526180945370264 40.0 37.0 41.0 32.0 41.0 54-55 37.33560938578242 40.0 37.0 41.0 31.0 41.0 56-57 37.13430265688326 40.0 36.5 41.0 31.0 41.0 58-59 36.92246381585048 40.0 36.0 41.0 31.0 41.0 60-61 36.652227968935776 39.5 35.5 41.0 31.0 41.0 62-63 36.363553251185245 39.0 35.0 41.0 31.0 41.0 64-65 36.03619054501826 39.0 35.0 41.0 31.0 41.0 66-67 35.69465924447381 38.0 35.0 40.5 30.0 41.0 68-69 35.314631820562695 37.0 35.0 40.0 30.0 41.0 70-71 34.90938757315655 37.0 35.0 39.5 30.0 41.0 72-73 34.4973461865862 36.0 35.0 39.0 29.5 41.0 74-75 34.0824546447011 36.0 34.0 39.0 29.0 40.5 76-77 33.65876960780865 35.0 34.0 37.0 29.0 39.5 78-79 33.23150213066138 35.0 34.0 37.0 28.5 39.0 80-81 32.82986270776587 35.0 34.0 36.5 27.5 39.0 82-83 32.65671920997647 35.0 34.0 36.0 28.0 37.5 84-85 32.31050928019268 35.0 34.0 36.0 27.5 37.0 86-87 31.74860785917617 35.0 34.0 35.5 14.0 37.0 88-89 16.570750556888306 18.0 17.0 18.5 2.0 19.0 90-91 2.811280897467957 2.0 2.0 2.0 2.0 2.0 92-93 2.3044319547403136 2.0 2.0 2.0 2.0 2.0 94-95 2.1040100434958067 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0033475099313413637 1101 2 0.1583538754955356 1101 3 0.08261577238256734 1101 4 0.09769404830962714 1101 5 0.11449988073712802 1101 6 0.12603095144567078 1101 7 0.15969687905274554 1101 8 0.2264975715639892 1101 9 0.1760231557221772 1101 10-11 0.16492202349932228 1101 12-13 0.18818956902732964 1101 14-15 0.15417421648011498 1101 16-17 0.3455583404347564 1101 18-19 0.21564169978672965 1101 20-21 0.2770208135542873 1101 22-23 0.29250601620472594 1101 24-25 0.25934341198332334 1101 26-27 0.3396174263657912 1101 28-29 0.32638332410591175 1101 30-31 0.23789760385564307 1101 32-33 0.3428188366042093 1101 34-35 0.32026296046996094 1101 36-37 0.3465117990166604 1101 38-39 0.26840465607590147 1101 40-41 0.29187353490964085 1101 42-43 0.316600575001317 1101 44-45 0.3257400376404789 1101 46-47 0.3207988647416613 1101 48-49 0.34753746586693524 1101 50-51 0.28927451056811293 1101 52-53 0.2630232510366781 1101 54-55 0.22378920574749372 1101 56-57 0.24372747971729325 1101 58-59 0.20639176936736447 1101 60-61 0.26391168722854275 1101 62-63 0.2342907805131773 1101 64-65 0.2606940140831 1101 66-67 0.12933293609875562 1101 68-69 0.21067005875543288 1101 70-71 0.1765996555961422 1101 72-73 0.19198956908944353 1101 74-75 0.2196706878336272 1101 76-77 0.2536895766580045 1101 78-79 0.20957137174465856 1101 80-81 0.16854091368598034 1101 82-83 0.21184347358611433 1101 84-85 0.1652375351589228 1101 86-87 0.20462178239378304 1101 88-89 0.9121730543828903 1101 90-91 0.052733242929197566 1101 92-93 -0.24327929561618022 1101 94-95 -0.048196716325158206 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.015146383306611 1102 2 0.04507022470536626 1102 3 0.08708369426407359 1102 4 0.08206947298577916 1102 5 0.10292370205532109 1102 6 0.06508362224767694 1102 7 -0.03015999138541048 1102 8 0.03599760444214439 1102 9 0.08182417713680934 1102 10-11 0.07396351572911186 1102 12-13 -0.04767216737755575 1102 14-15 -0.0679106968311487 1102 16-17 0.019392371515571938 1102 18-19 -0.0525502209550055 1102 20-21 0.008524221922868946 1102 22-23 -0.058804726183986133 1102 24-25 0.03941815498575352 1102 26-27 -0.020295117745732227 1102 28-29 -0.0025755343645954554 1102 30-31 -0.0067533397473624746 1102 32-33 0.00655598672585711 1102 34-35 -5.243617140280321E-4 1102 36-37 0.05548534306243624 1102 38-39 0.08303105065758842 1102 40-41 0.12057068327779064 1102 42-43 0.046659536490039955 1102 44-45 0.0016745443585790554 1102 46-47 0.0013154901279790465 1102 48-49 -0.05264336397766556 1102 50-51 -0.0739407536626473 1102 52-53 -0.03650439949046813 1102 54-55 -0.0276363909909918 1102 56-57 -0.09591086059350573 1102 58-59 -0.14723008802912574 1102 60-61 -0.092095370615084 1102 62-63 -0.08020541658039804 1102 64-65 -0.12474030632546373 1102 66-67 -0.06823802591602401 1102 68-69 0.003694410507840473 1102 70-71 0.0756133109892474 1102 72-73 0.11897069702929741 1102 74-75 0.051861687987063476 1102 76-77 -0.05028341941853398 1102 78-79 0.05224063943859392 1102 80-81 0.09227587193073106 1102 82-83 0.012896670438578894 1102 84-85 -0.059750519111943845 1102 86-87 0.035799916229787954 1102 88-89 1.208237320213719 1102 90-91 0.1094789632895421 1102 92-93 -0.1710644040044933 1102 94-95 4.2985360667424644E-4 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.03637924901387635 1103 2 0.1376423686537649 1103 3 0.13326133812759622 1103 4 0.06531170690192312 1103 5 0.1657216141245783 1103 6 0.12621713098982212 1103 7 0.13347394173290894 1103 8 0.09992944962971961 1103 9 0.14348688295925172 1103 10-11 0.15880798186389455 1103 12-13 0.09888444440312583 1103 14-15 0.0945453802355587 1103 16-17 0.028317426684466795 1103 18-19 0.08309992333289529 1103 20-21 0.12092173507853232 1103 22-23 0.1200401218893532 1103 24-25 0.11890168919046573 1103 26-27 0.14970870163976713 1103 28-29 0.14508594076696113 1103 30-31 0.18509248439114856 1103 32-33 0.21717430280123295 1103 34-35 0.1851206900598612 1103 36-37 0.1554683680157467 1103 38-39 0.14026454242468134 1103 40-41 0.16048538366821674 1103 42-43 0.22995227117005612 1103 44-45 0.1257619234113747 1103 46-47 0.179159659199442 1103 48-49 0.05250007516679034 1103 50-51 0.02922339503876259 1103 52-53 0.07177730140920602 1103 54-55 0.12517488892753192 1103 56-57 0.07809970637305241 1103 58-59 0.034645654629166245 1103 60-61 -0.0010463127301818531 1103 62-63 -0.07953999475049045 1103 64-65 -0.023502825422390572 1103 66-67 -0.026891480338015583 1103 68-69 -0.02393035608526617 1103 70-71 -0.04160363010733903 1103 72-73 -0.09677705377701784 1103 74-75 -0.09498413615145296 1103 76-77 -0.11759457202420265 1103 78-79 -0.14291616755512848 1103 80-81 -0.08918016269836215 1103 82-83 0.0071951766344682255 1103 84-85 -0.062208081495242595 1103 86-87 -0.11217453632495378 1103 88-89 0.8046664069562084 1103 90-91 -0.6045394546361069 1103 92-93 -0.3410644040044932 1103 94-95 -0.11794223941658144 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.02162850417368034 1104 2 0.05615386000575029 1104 3 0.08374851606000533 1104 4 0.1277914265994582 1104 5 0.12052460430153644 1104 6 0.14134848999132998 1104 7 -0.035956923330566326 1104 8 0.13706964838487323 1104 9 0.16076529095141012 1104 10-11 0.23582324985887482 1104 12-13 0.1852101124649721 1104 14-15 0.2698861098020302 1104 16-17 0.19542896261214793 1104 18-19 0.20226165444312016 1104 20-21 0.10728405108088168 1104 22-23 0.013063779783998086 1104 24-25 0.14134952579432536 1104 26-27 0.1597027937213582 1104 28-29 0.13111803608683914 1104 30-31 0.07607756218557427 1104 32-33 0.18504190479271188 1104 34-35 0.18191640227131245 1104 36-37 0.08252401224925876 1104 38-39 -0.02148070256136947 1104 40-41 0.1574980336605094 1104 42-43 0.09695548160339484 1104 44-45 0.008596777835677472 1104 46-47 0.15299028466855447 1104 48-49 0.06516973321221542 1104 50-51 0.029149648484370516 1104 52-53 0.00985006631502472 1104 54-55 0.05394462842191672 1104 56-57 0.07081139585744722 1104 58-59 0.004909405083935781 1104 60-61 -0.03747219416538883 1104 62-63 -0.13543332773420502 1104 64-65 -0.19841654195374758 1104 66-67 -0.13679453639523587 1104 68-69 -0.1636524217098696 1104 70-71 -0.17643240699614893 1104 72-73 -0.03790026296485394 1104 74-75 -0.021162298813408142 1104 76-77 0.010971893287056389 1104 78-79 0.013015902854803585 1104 80-81 0.024563859733696347 1104 82-83 0.02251540875584368 1104 84-85 0.10536273158510312 1104 86-87 0.12093238142960772 1104 88-89 0.8144039224839545 1104 90-91 -0.5832625494348447 1104 92-93 -0.3410644040044932 1104 94-95 -0.11794223941658144 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.0940415362244238 1105 2 0.03079660188085498 1105 3 0.05411673027666808 1105 4 0.12296386506442758 1105 5 0.09956522638938736 1105 6 0.14323611640081424 1105 7 0.033357437819518054 1105 8 0.1174016602884933 1105 9 0.2098504196573927 1105 10-11 0.15795426200079277 1105 12-13 0.26181077802139896 1105 14-15 0.0586078156877079 1105 16-17 0.246851241021794 1105 18-19 0.22398783171227876 1105 20-21 0.2725920151010257 1105 22-23 0.11233052606616667 1105 24-25 0.15175932800212166 1105 26-27 0.1818247352475595 1105 28-29 0.18260462985364256 1105 30-31 0.17014747121941554 1105 32-33 0.052317293182206015 1105 34-35 0.2328316840888931 1105 36-37 0.033421680651393615 1105 38-39 0.12867552120055592 1105 40-41 0.14961969563971422 1105 42-43 0.12392199206909993 1105 44-45 0.0927709188004684 1105 46-47 0.1396134723292306 1105 48-49 0.14442257642994605 1105 50-51 0.10105939820379461 1105 52-53 0.12304597460718725 1105 54-55 0.04707764450526497 1105 56-57 0.11915621375017338 1105 58-59 -0.03638955791400633 1105 60-61 -0.07042433356980382 1105 62-63 -0.033141804791512186 1105 64-65 -0.021804323091515698 1105 66-67 0.05222810280597656 1105 68-69 0.14085714045894093 1105 70-71 -0.02888750402730267 1105 72-73 0.011780124897804 1105 74-75 0.06879631454749102 1105 76-77 0.17450492938830564 1105 78-79 0.014368186998098054 1105 80-81 0.04160948199845649 1105 82-83 0.137954632795946 1105 84-85 0.08931155376369304 1105 86-87 0.11328794409222098 1105 88-89 1.1190139651803612 1105 90-91 -0.822148943687202 1105 92-93 -0.3410644040044932 1105 94-95 -0.11794223941658144 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.15552248572961602 1106 2 -0.1799294227812105 1106 3 -0.14577116085291308 1106 4 -0.18600731450906238 1106 5 -0.21568638788595962 1106 6 -0.1550720879661469 1106 7 -0.2796391779858709 1106 8 -0.2265048379722714 1106 9 -0.26162300124504867 1106 10-11 -0.26242543750746705 1106 12-13 -0.26612292125268766 1106 14-15 -0.24225960454313622 1106 16-17 -0.3401362676819133 1106 18-19 -0.3202823510425361 1106 20-21 -0.36367173525178487 1106 22-23 -0.3717268610052642 1106 24-25 -0.32107868750118485 1106 26-27 -0.43273823718735116 1106 28-29 -0.20935275595453362 1106 30-31 -0.2125356677459962 1106 32-33 -0.19571792627382933 1106 34-35 -0.27333882886480865 1106 36-37 -0.18471981974841611 1106 38-39 -0.1909076321508394 1106 40-41 -0.15811736248852526 1106 42-43 -0.5122491826776283 1106 44-45 -0.25404916569409863 1106 46-47 -0.29931792612993746 1106 48-49 -0.20623043982848088 1106 50-51 -0.29845977427964243 1106 52-53 -0.3518160717134151 1106 54-55 -0.25923005380686703 1106 56-57 -0.42645433557009227 1106 58-59 -0.40135691228607584 1106 60-61 -0.3272863825300476 1106 62-63 -0.2781171270762286 1106 64-65 -0.2658692557347493 1106 66-67 -0.26750560374595267 1106 68-69 -0.32527715676683044 1106 70-71 -0.29301551252238056 1106 72-73 -0.31386079148379764 1106 74-75 -0.3712949186180339 1106 76-77 -0.44001430060136215 1106 78-79 -0.31297736493257844 1106 80-81 -0.3331815394121449 1106 82-83 -0.25179357098275545 1106 84-85 -0.2375334111320484 1106 86-87 -0.17878215393045238 1106 88-89 0.7603392066641135 1106 90-91 -0.1751155959176045 1106 92-93 -0.3222250796216435 1106 94-95 -0.11794223941658144 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 0.018186150947407498 1107 2 -0.010913621555598496 1107 3 0.07282264824979023 1107 4 0.07930145420606038 1107 5 0.10777897489833776 1107 6 0.09114246801129866 1107 7 -0.008846604869404473 1107 8 -0.001477876489680341 1107 9 -0.01861826024755686 1107 10-11 0.035826169159797416 1107 12-13 0.06262146462754004 1107 14-15 0.13736751750430187 1107 16-17 0.06660386215555292 1107 18-19 0.10522486011210219 1107 20-21 0.029437146903475764 1107 22-23 0.15379300194779688 1107 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0.016971804589523742 2216 4 0.026884080893076145 2216 5 0.05812282098398924 2216 6 -0.009437135601359614 2216 7 0.015953877507342895 2216 8 -0.01636133635688708 2216 9 -0.11118662875615115 2216 10-11 -0.10133137182256036 2216 12-13 -0.1762991645523755 2216 14-15 -0.15127472932863384 2216 16-17 -0.2653031640704526 2216 18-19 -0.18223067298291085 2216 20-21 -0.23285755969274646 2216 22-23 -0.25644402962686286 2216 24-25 -0.3126823701455308 2216 26-27 -0.2971984393208089 2216 28-29 -0.32729772555069303 2216 30-31 -0.3136715054590127 2216 32-33 -0.4056905855555115 2216 34-35 -0.3280193737649171 2216 36-37 -0.31646801761816334 2216 38-39 -0.36893938876078636 2216 40-41 -0.29647453547370617 2216 42-43 -0.2747760351688342 2216 44-45 -0.3447512110164297 2216 46-47 -0.33946550299263833 2216 48-49 -0.3334106302623212 2216 50-51 -0.3024863475079158 2216 52-53 -0.32585413054025736 2216 54-55 -0.2857890142531829 2216 56-57 -0.37687461216970064 2216 58-59 -0.4394962928782107 2216 60-61 -0.34454034356045327 2216 62-63 -0.4198189901865703 2216 64-65 -0.34200675059609864 2216 66-67 -0.3669146631604363 2216 68-69 -0.35225441712459826 2216 70-71 -0.31302613610656493 2216 72-73 -0.292206618576202 2216 74-75 -0.2968893295545598 2216 76-77 -0.3677097972895922 2216 78-79 -0.2823980734311178 2216 80-81 -0.30484021560179286 2216 82-83 -0.2889484716980206 2216 84-85 -0.3621629307254892 2216 86-87 -0.4649276060763192 2216 88-89 -1.1719254864453905 2216 90-91 -0.822148943687202 2216 92-93 -0.3410644040044932 2216 94-95 -0.11794223941658144 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13700.0 3 2618.0 4 2147.0 5 1800.0 6 2358.0 7 3022.0 8 3134.0 9 3428.0 10 4175.0 11 5234.0 12 6148.0 13 6782.0 14 7341.0 15 7993.0 16 8799.0 17 10058.0 18 12395.0 19 14774.0 20 14409.0 21 20111.0 22 22654.0 23 28950.0 24 45442.0 25 79556.0 26 170502.0 27 502169.0 28 543858.0 29 19394.0 30 648.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.415735108554045 19.254936847618858 14.096453118734406 23.232874925092688 2 14.811657305706241 20.674462421638466 40.21601626005861 24.297864012596683 3 18.874844439325738 25.74293218674319 29.105410152313492 26.276813221617573 4 12.485010820364039 17.04319315277637 37.83147362992179 32.64032239693779 5 13.61360099515731 38.78502759206741 33.76764817298537 13.833723239789911 6 31.172826280907064 36.765756437552085 17.956202325532313 14.105214956008542 7 28.321711640900254 33.07823809045669 21.216693026792672 17.383357241850373 8 26.012935541657423 35.12473466662488 20.32164256948233 18.54068722223537 9 26.433311865766097 17.705690525511976 20.232041591226395 35.62895601749553 10-11 24.679127532595693 27.42403132281927 27.23939194999196 20.657449194593077 12-13 25.67922221657276 24.686239164630464 27.449975904523715 22.18456271427306 14-15 22.5451026765814 26.202434255841812 25.443160298772256 25.809302768804532 16-17 22.308821116983975 29.292121454763365 26.22818767909455 22.170869749158108 18-19 22.38114776248325 28.49444398429023 27.37540578406388 21.74900246916265 20-21 23.557067196906566 27.39696809031133 26.819155406636995 22.226809306145107 22-23 23.232132658562684 27.38545132908628 26.71676708708724 22.6656489252638 24-25 23.08203701844271 27.60573523006858 26.81969609855212 22.49253165293659 26-27 22.71058628203267 27.602953186846502 27.066434552593087 22.620025978527742 28-29 22.452110146662676 28.057998654376988 26.916265665891526 22.573625533068814 30-31 22.68516416293436 27.664893620423136 26.894139737873967 22.755802478768533 32-33 22.55652249547673 27.79321953899414 26.743929479828676 22.906328485700453 34-35 23.164738262411994 27.68015574330623 26.539709522617223 22.61539647166456 36-37 22.983098607763246 27.31739403772962 26.785192367096677 22.914314987410457 38-39 22.68756247605684 27.674646760454568 26.76031386563946 22.877476897849128 40-41 22.961865542252202 27.531067748188637 26.78001201075212 22.72705469880705 42-43 22.705821633296004 27.69885373423749 26.52793331282509 23.067391319641416 44-45 22.70128808642372 27.529285810903502 26.70340243994862 23.06602366272416 46-47 22.987959189024807 27.852505661617844 26.3927963627503 22.766738786607053 48-49 22.849823659974092 27.723517258426632 26.440003619855094 22.986655461744178 50-51 22.508584362103072 27.796001404452163 26.828521890842854 22.866892342601908 52-53 22.959595139162918 27.82094386092598 26.409008959458273 22.810452040452827 54-55 22.52845084402785 27.462147618213145 26.655214271918055 23.35418726584095 56-57 22.662268266991727 27.797856099933547 26.51440682681429 23.02546880626043 58-59 22.968516863978554 27.426053610932215 26.474530873964486 23.130898651124745 60-61 22.619834113477943 27.640974444214915 26.961964032977765 22.777227409329374 62-63 22.524413228711452 27.685647023309684 26.88224410478646 22.907695643192405 64-65 22.378858767132357 27.85385762393607 26.543450892284685 23.22383271664689 66-67 22.47088287981765 27.82196714119157 26.297631298050206 23.409518680940575 68-69 22.489288310179965 27.561572030617892 26.793246718860473 23.15589294034167 70-71 22.704542532964016 27.589042970736106 26.568704635907288 23.137709860392594 72-73 22.691975372202208 27.413710292728506 26.745412346771776 23.14890198829751 74-75 22.428448726303866 28.08590949469781 26.44719010436819 23.03845167463013 76-77 22.750046527274577 27.558632360343026 26.543634269400272 23.14768684298212 78-79 22.765156033201723 27.66831660530694 26.642186732037448 22.924340629453884 80-81 22.83878411280642 27.80610629707489 26.534168926943547 22.82094066317515 82-83 22.765910500834618 27.567297053575423 26.861293561611422 22.805498883978537 84-85 22.623806559036737 27.662887883302524 26.744077843512898 22.96922771414785 86-87 22.79780568022474 27.63410309337691 26.595238818546513 22.97285240785184 88-89 32.286531324570866 17.53476620404234 32.48206334650756 17.69663912487923 90-91 31.429704412041943 14.051836779800947 15.96408999603456 38.554368812122554 92-93 23.42647230520562 16.91384948590758 33.56516318243506 26.094515026451738 94-95 15.881797758013711 6.7665485454882335 27.269895312210483 50.08175838428757 96-97 27.43521205295049 7.134432285607254 15.146612827648791 50.28374283379347 98-99 57.804833310404916 14.1752648232219 18.716925757784196 9.302976108588986 100-101 31.820119689662178 6.901162314915275 12.163587310889541 49.11513068453301 102-103 24.115145536349342 2.0012780928347773 36.416652813848096 37.466923556967785 104-105 43.47154628491308 4.547971510724119 38.29148166587078 13.689000538492019 106-107 5.782409677661418 1.855222492325895 7.00468212016453 85.35768570984817 108-109 19.604141063767962 9.89427756457786 20.111892749259084 50.38968862239509 110-111 41.11970379662915 2.570818735661711 23.83789925912384 32.47157820858529 112-113 28.31528652250507 2.7861233374669356 38.07440217998733 30.82418796004066 114-115 28.388583936445063 3.8002697356155126 23.044235188149067 44.76691113979036 116-117 19.823728503388846 4.154303801932372 20.210507855204604 55.811459839474175 118-119 20.60777621643987 4.264788840667037 15.774122764871931 59.353312178021156 120-121 25.91612888513899 4.231832974450355 29.544647539995633 40.30739060041502 122-123 34.67248607696819 1.5861732082080688 33.66401774946618 30.07732296535756 124-125 39.121141687885576 1.7794543125838762 18.475950768728346 40.623453230802205 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 34.0 1 92.0 2 187.5 3 284.0 4 374.0 5 400.5 6 381.0 7 346.5 8 313.0 9 284.0 10 274.0 11 259.0 12 244.5 13 255.0 14 248.0 15 264.5 16 345.0 17 451.0 18 601.5 19 792.0 20 1125.5 21 1651.0 22 2469.0 23 3676.5 24 5256.5 25 7211.0 26 9397.0 27 11932.5 28 14895.5 29 17958.0 30 21268.5 31 24909.5 32 28569.5 33 31953.5 34 35160.0 35 38601.0 36 41775.5 37 44598.0 38 47359.5 39 50097.0 40 52852.5 41 55256.0 42 57967.5 43 61026.5 44 63517.0 45 64791.5 46 64469.5 47 63185.5 48 62125.5 49 61108.0 50 59235.0 51 56875.0 52 53715.5 53 49806.5 54 45236.0 55 40733.0 56 36760.5 57 32403.5 58 27745.5 59 23269.0 60 19332.5 61 15871.0 62 12743.5 63 9966.5 64 7544.0 65 5498.5 66 3998.5 67 2947.0 68 2143.5 69 1524.0 70 1063.0 71 730.0 72 522.0 73 392.5 74 292.5 75 211.0 76 138.5 77 86.0 78 53.0 79 32.0 80 19.0 81 18.5 82 18.5 83 10.0 84 6.0 85 6.0 86 4.5 87 5.5 88 8.5 89 8.5 90 6.5 91 7.0 92 6.0 93 3.0 94 5.5 95 7.0 96 4.5 97 4.5 98 3.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 8.314152157938193E-4 3 0.04502433168606529 4 0.051483788362617265 5 0.0531466187942049 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.715276742950078E-4 12-13 0.0017587629564869253 14-15 0.0 16-17 2.8779757469786054E-4 18-19 0.007258894384046036 20-21 0.0019186504979857367 22-23 3.837300995971473E-4 24-25 0.0 26-27 0.0 28-29 7.035051825947701E-4 30-31 0.0 32-33 0.0011511902987914422 34-35 3.1977508299762275E-5 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 3.1977508299762275E-5 46-47 0.0 48-49 3.1977508299762275E-5 50-51 0.0 52-53 0.0 54-55 0.001598875414988114 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 3.1977508299762275E-5 66-67 0.0 68-69 2.2384255809833597E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.5175259129738507E-4 80-81 0.0 82-83 5.755951493957211E-4 84-85 3.1977508299762275E-5 86-87 0.0010552577738921552 88-89 19.341563917602915 90-91 26.213978136338024 92-93 40.292427917899666 94-95 44.773564065978555 96-97 48.27119996878995 98-99 93.02666476507085 100-101 51.546016593768606 102-103 53.06302958750933 104-105 74.82142160489997 106-107 81.67589644147893 108-109 12.623441176414158 110-111 39.12899023342942 112-113 51.93371190439493 114-115 86.79338500472308 116-117 66.44993377458032 118-119 71.71368746078758 120-121 80.36766459942734 122-123 75.84409429783467 124-125 49.8661741277655 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1563599.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.12789786523902 #Duplication Level Percentage of deduplicated Percentage of total 1 67.38493502022448 29.061705952080096 2 14.607072901624008 12.599446964226816 3 6.278464385820523 8.123309123466244 4 3.3970824853455337 5.860361058710981 5 2.0501984866956944 4.421037546883975 6 1.359466433290002 3.517855769169119 7 0.9401951855797033 2.8384049355949625 8 0.700852563330831 2.4181038215938435 9 0.5220962816085454 2.0265223598130975 >10 2.5650394209701304 19.215384068128404 >50 0.1334399235201665 3.9154811033578354 >100 0.05640696381423665 4.220722518101561 >500 0.004007768773702533 1.1847036900515462 >1k 7.421794025375061E-4 0.5969610888214993 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3435 0.21968548201936686 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1966 0.1257355626346653 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1583 0.10124079127704737 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.197750829976228E-5 0.0 22-23 0.0 0.0 0.0 9.593252489928684E-5 0.0 24-25 0.0 0.0 0.0 2.558200663980982E-4 0.0 26-27 0.0 0.0 0.0 7.674601991942947E-4 0.0 28-29 0.0 0.0 0.0 0.0026861106971800314 0.0 30-31 0.0 0.0 0.0 0.00786646704174152 0.0 32-33 0.0 0.0 0.0 0.019218482488157128 0.0 34-35 0.0 0.0 0.0 0.03581480929573375 0.0 36-37 0.0 0.0 0.0 0.06325151141692978 0.0 38-39 0.0 0.0 0.0 0.1064851026382084 0.0 40-41 0.0 0.0 0.0 0.15924799133281614 0.0 42-43 0.0 0.0 0.0 0.2121068125523232 0.0 44-45 0.0 0.0 0.0 0.27184079805627914 0.0 46-47 0.0 0.0 0.0 0.34391810176394333 0.0 48-49 0.0 0.0 0.0 0.4249810853038407 0.0 50-51 0.0 0.0 0.0 0.5115442002712972 0.0 52-53 0.0 0.0 0.0 0.5975636975976577 0.0 54-55 0.0 0.0 0.0 0.6875164284448889 0.0 56-57 0.0 0.0 0.0 0.7968475293217763 0.0 58-59 0.0 0.0 0.0 0.9165073653794866 0.0 60-61 0.0 0.0 0.0 1.0444813535951354 0.0 62-63 0.0 0.0 0.0 1.1759089127071582 0.0 64-65 0.0 0.0 0.0 1.315170961352623 0.0 66-67 0.0 0.0 0.0 1.4728840322870504 0.0 68-69 0.0 0.0 0.0 1.6543563918882014 0.0 70-71 0.0 0.0 0.0 1.854599548861313 0.0 72-73 0.0 0.0 0.0 2.0525083477285415 0.0 74-75 0.0 0.0 0.0 2.263016284865877 0.0 76-77 0.0 0.0 0.0 2.493829939773561 0.0 78-79 0.0 6.395501659952456E-5 0.0 2.5577849563730854 0.0 80-81 0.0 1.2791003319904913E-4 0.0 2.5588402141469775 0.0 82-83 0.0 1.2791003319904913E-4 0.0 2.558904169163577 6.395501659952456E-5 84-85 0.0 1.2791003319904913E-4 0.0 2.558904169163577 6.395501659952456E-5 86-87 0.0 1.2791003319904913E-4 0.0 2.558904169163577 6.395501659952456E-5 88-89 0.0 1.2791003319904913E-4 0.0 2.558904169163577 6.395501659952456E-5 90-91 0.0 1.2791003319904913E-4 0.0 2.558904169163577 6.395501659952456E-5 92-93 0.0 1.2791003319904913E-4 0.0 2.558904169163577 6.395501659952456E-5 94-95 0.0 1.2791003319904913E-4 0.0 2.558904169163577 6.395501659952456E-5 96-97 0.0 1.2791003319904913E-4 0.0 2.558904169163577 6.395501659952456E-5 98-99 0.0 1.2791003319904913E-4 0.0 2.558904169163577 6.395501659952456E-5 100-101 0.0 1.2791003319904913E-4 0.0 2.558904169163577 6.395501659952456E-5 102-103 0.0 1.2791003319904913E-4 0.0 2.558904169163577 6.395501659952456E-5 104-105 0.0 1.2791003319904913E-4 0.0 2.558904169163577 6.395501659952456E-5 106-107 0.0 1.2791003319904913E-4 0.0 2.558904169163577 6.395501659952456E-5 108-109 0.0 1.2791003319904913E-4 0.0 2.558904169163577 6.395501659952456E-5 110-111 0.0 1.2791003319904913E-4 0.0 2.558904169163577 6.395501659952456E-5 112-113 0.0 1.2791003319904913E-4 0.0 2.558904169163577 6.395501659952456E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCTCG 170 0.0 1143.833 108-109 CCGCGCC 775 0.0 972.25806 108-109 CCGCTCC 700 0.0 868.0876 108-109 CGTCGCG 90 1.2655437E-7 810.215 108-109 CTCCGCC 460 0.0 803.16974 110-111 CCTCGCC 685 0.0 780.6452 108-109 CGTCGCT 150 3.778805E-7 615.7634 110-111 CGTCGCC 400 0.0 546.8952 108-109 CGGCGCG 90 1.3748775E-4 540.1434 108-109 TGCGTCG 60 1.2139384E-5 513.13617 110-111 CGGCGCC 335 0.0 507.896 108-109 GGTCGCC 195 4.425601E-9 498.59387 108-109 CGTAGCT 125 1.809393E-4 492.61075 110-111 AAGCGCG 105 2.1822502E-4 462.98 108-109 CGTTGGT 140 2.5411448E-4 439.83105 110-111 TTTTTCG 840 0.0 398.84003 92-93 TTGCTCG 190 2.5055433E-6 383.7861 108-109 CGCCTCT 345 2.3355824E-8 356.96432 110-111 CCGCGCG 485 0.0 350.81476 108-109 AATCTCG 140 5.1671884E-4 347.23505 108-109 >>END_MODULE