FastQCFastQC Report
Fri 27 May 2016
SRR939968_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939968_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563599
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73970.47307525778668313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56050.35846786804033515No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36330.23234857530607272No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33650.21520863085740016No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19340.1236890021034805No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA149450.032.731511
GTACATG72900.030.7311731
TACATGG74550.029.8873312
GAGTACT84400.029.6064512-13
ACATGGG72100.028.8372173
ATCAACG171900.028.2798773
CAACGCA172700.028.1833695
TCAACGC172900.028.150774
TATCAAC173850.028.1340122
AACGCAG174900.027.8255026
AGTACTT86800.027.6911612-13
GGTATCA110350.026.8890721
GTACTTT92300.026.78278714-15
AGAGTAC131750.026.30418210-11
TATATCG1159.976265E-425.9021225
ACGCAGA189850.025.4463187
CGCAGAG191500.025.1968
GCAGAGT199700.023.8336969
TACTTTT102900.023.18544414-15
ATACGGC1300.00205351822.882646
Produced by FastQC (version 0.11.5)