##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939968_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1563599 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.84213343702574 34.0 31.0 34.0 31.0 34.0 2 32.909009918783525 34.0 31.0 34.0 31.0 34.0 3 32.948694646133696 34.0 33.0 34.0 31.0 34.0 4 36.373258744729306 37.0 37.0 37.0 35.0 37.0 5 36.26818832705828 37.0 37.0 37.0 35.0 37.0 6 36.39859580365554 37.0 37.0 37.0 35.0 37.0 7 36.39229111811916 37.0 37.0 37.0 35.0 37.0 8 36.412220780391905 37.0 37.0 37.0 35.0 37.0 9 38.24498160973498 39.0 39.0 39.0 37.0 39.0 10-11 38.24925572349432 39.0 39.0 39.0 37.0 39.0 12-13 38.234582843810976 39.0 39.0 39.0 37.0 39.0 14-15 39.751689211875934 41.0 40.0 41.0 37.0 41.0 16-17 39.773036437091605 41.0 40.0 41.0 37.0 41.0 18-19 39.8311504420251 41.0 40.0 41.0 37.5 41.0 20-21 39.84636917777512 41.0 40.0 41.0 37.5 41.0 22-23 39.791023145960054 41.0 40.0 41.0 37.0 41.0 24-25 39.74035766203483 41.0 40.0 41.0 37.0 41.0 26-27 39.640561614582765 41.0 40.0 41.0 37.0 41.0 28-29 39.54637218366091 41.0 40.0 41.0 36.5 41.0 30-31 39.38521097800651 41.0 39.0 41.0 36.0 41.0 32-33 39.184109224935554 41.0 39.0 41.0 35.0 41.0 34-35 39.03946312321765 41.0 39.0 41.0 35.0 41.0 36-37 38.997075337090905 41.0 39.0 41.0 35.0 41.0 38-39 39.025810965599234 41.0 39.0 41.0 35.0 41.0 40-41 38.94121926401846 41.0 39.0 41.0 35.0 41.0 42-43 38.76891037919569 41.0 39.0 41.0 35.0 41.0 44-45 38.53747636062699 41.0 39.0 41.0 35.0 41.0 46-47 38.20822826057064 41.0 39.0 41.0 34.5 41.0 48-49 37.8565840730264 41.0 38.5 41.0 34.0 41.0 50-51 37.63300245139578 41.0 38.0 41.0 33.0 41.0 52-53 37.476312660726954 41.0 38.0 41.0 33.0 41.0 54-55 37.317981784332176 40.0 37.0 41.0 33.0 41.0 56-57 37.14364424638286 40.0 37.0 41.0 33.0 41.0 58-59 36.93753289686167 40.0 36.5 41.0 32.5 41.0 60-61 36.70694148563666 40.0 36.0 41.0 32.0 41.0 62-63 36.45177919658429 39.0 35.0 41.0 32.0 41.0 64-65 36.16677165948559 39.0 35.0 41.0 31.5 41.0 66-67 35.85393057938768 38.5 35.0 41.0 31.0 41.0 68-69 35.506881240011026 37.0 35.0 40.5 31.0 41.0 70-71 35.13678890815356 37.0 35.0 39.5 31.0 41.0 72-73 34.77410384631865 36.0 35.0 39.0 30.5 41.0 74-75 34.415846070507854 36.0 35.0 39.0 30.0 41.0 76-77 34.05602843184218 35.5 35.0 37.5 30.0 39.5 78-79 33.715963300053275 35.0 35.0 37.0 30.0 39.0 80-81 33.466383644399876 35.0 35.0 37.0 29.5 39.0 82-83 33.27580025313395 35.0 35.0 36.0 30.0 38.0 84-85 33.062085291689236 35.0 35.0 36.0 30.0 37.0 86-87 32.89410616149026 35.0 35.0 35.5 30.0 37.0 88-89 32.735247336433446 35.0 34.5 35.0 29.5 36.0 90-91 32.61452456799985 35.0 34.0 35.0 29.0 36.0 92-93 32.504175303258705 35.0 34.0 35.0 29.0 36.0 94-95 32.42769469665816 35.0 34.0 35.0 29.0 36.0 96-97 32.37984067526264 35.0 34.0 35.0 29.0 36.0 98-99 32.322763381148235 35.0 34.0 35.0 29.0 35.0 100-101 32.27337635800483 35.0 34.0 35.0 29.0 35.0 102-103 31.45554039111051 34.5 32.5 35.0 26.0 35.0 104-105 32.127174230733075 35.0 34.0 35.0 29.0 35.0 106-107 32.19887931624413 35.0 34.0 35.0 29.0 35.0 108-109 32.19216499882643 35.0 34.0 35.0 29.0 35.0 110-111 32.15938197709259 35.0 34.0 35.0 29.0 35.0 112-113 32.09563545384718 35.0 34.0 35.0 29.0 35.0 114-115 32.04429300607125 35.0 34.0 35.0 29.0 35.0 116-117 31.990365176749282 35.0 34.0 35.0 28.0 35.0 118-119 31.91105551998946 35.0 34.0 35.0 27.0 35.0 120-121 31.81693835823635 35.0 34.0 35.0 27.0 35.0 122-123 31.66072599176643 35.0 34.0 35.0 26.0 35.0 124-125 29.725603879255488 33.5 30.5 34.5 13.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.093722328702043 1101 2 0.08132433265525663 1101 3 0.08456281460091475 1101 4 0.04365050174248353 1101 5 -0.0583648679902069 1101 6 0.029145320332617075 1101 7 0.04965068901447722 1101 8 0.1012099524994099 1101 9 0.018203054449202227 1101 10-11 0.05005481891940633 1101 12-13 0.015034835775637134 1101 14-15 0.15311272454994906 1101 16-17 0.15794996060458288 1101 18-19 0.1663097510602185 1101 20-21 0.17580487355711938 1101 22-23 0.14564266454892305 1101 24-25 0.16994457662966056 1101 26-27 0.13723550066024615 1101 28-29 0.0679740622002214 1101 30-31 0.14975742553956906 1101 32-33 0.17949063013751498 1101 34-35 0.11240744109093725 1101 36-37 0.02075924634375781 1101 38-39 -0.06536345638971142 1101 40-41 -0.03604796308400893 1101 42-43 -0.030512004091320932 1101 44-45 -0.04004746516477553 1101 46-47 -0.07855658223823525 1101 48-49 0.022147201793295324 1101 50-51 -0.01441005958383812 1101 52-53 -0.05029143435984906 1101 54-55 0.048478368479287326 1101 56-57 0.08481351043928953 1101 58-59 0.12451250222152765 1101 60-61 0.049726349039531215 1101 62-63 0.028623022668710973 1101 64-65 -0.0396466881394133 1101 66-67 -0.013236114178873493 1101 68-69 -0.03439548455477137 1101 70-71 0.05689368018956742 1101 72-73 0.02888966416860228 1101 74-75 0.0014052599179663616 1101 76-77 -0.055361264123384046 1101 78-79 -0.10582104527704672 1101 80-81 -0.06258371570533683 1101 82-83 -0.04847929794627248 1101 84-85 -0.07439715115133794 1101 86-87 -0.062223796837265866 1101 88-89 -0.04667966564026216 1101 90-91 0.02070651543466795 1101 92-93 -0.008687866910719322 1101 94-95 -0.0028626510355493906 1101 96-97 -0.007449255226177343 1101 98-99 0.03302326816450574 1101 100-101 -0.014596975071370366 1101 102-103 0.00984166615524984 1101 104-105 0.002086507080498734 1101 106-107 0.037610532076641334 1101 108-109 0.09036819798206608 1101 110-111 0.028996906440546866 1101 112-113 -0.033265086081030404 1101 114-115 0.029571569833223066 1101 116-117 -0.04891093351264786 1101 118-119 0.014189491577131008 1101 120-121 -0.039308598176134524 1101 122-123 -0.07451164990290238 1101 124-125 0.06079284094279913 1102 1 -0.03669171684516215 1102 2 0.0385029487041777 1102 3 0.054230745249931545 1102 4 -0.013023544243097263 1102 5 -0.10855621884394395 1102 6 -0.047663745270256186 1102 7 0.011733410448840687 1102 8 -3.817885084202999E-4 1102 9 -0.04260267950077434 1102 10-11 0.07020255142274578 1102 12-13 0.024661120763468602 1102 14-15 0.06142366420758094 1102 16-17 0.10077945450019854 1102 18-19 0.043209779554622685 1102 20-21 0.028677109052374306 1102 22-23 0.08211637298545327 1102 24-25 0.08442718408642946 1102 26-27 0.015046034820642262 1102 28-29 0.05733863708569231 1102 30-31 0.0029897864101755545 1102 32-33 -0.08966966182050129 1102 34-35 -0.04032845966087706 1102 36-37 0.02064746062164602 1102 38-39 0.015068562548108844 1102 40-41 -0.09340434034987055 1102 42-43 -0.06669353533652611 1102 44-45 -0.06975791822572575 1102 46-47 -0.10661918224262479 1102 48-49 -0.16369349259331045 1102 50-51 -0.06714771383717988 1102 52-53 -0.06346855993247402 1102 54-55 -0.14696713050149413 1102 56-57 -0.12808458920343213 1102 58-59 -0.17494895362311524 1102 60-61 -0.1759753803513462 1102 62-63 -0.17580785649310826 1102 64-65 -0.1838993677089249 1102 66-67 -0.16422552741342145 1102 68-69 -0.20705795743029398 1102 70-71 -0.22408433528509164 1102 72-73 -0.20374782131020908 1102 74-75 -0.13272971586179239 1102 76-77 -0.18552762758042007 1102 78-79 -0.031835993877535884 1102 80-81 -0.08893993953981294 1102 82-83 -0.11988385266648294 1102 84-85 -0.09335096830496781 1102 86-87 -0.007282648732136465 1102 88-89 -0.10294207714323278 1102 90-91 -0.022261285893613092 1102 92-93 -0.121879897246302 1102 94-95 -0.13163651509534446 1102 96-97 -0.15603154924454543 1102 98-99 -0.1937654129831614 1102 100-101 -0.10875365043248308 1102 102-103 -0.08077008668870889 1102 104-105 -0.04866552588532613 1102 106-107 -0.055868634133375394 1102 108-109 -0.10868819901067894 1102 110-111 -0.13823869840788205 1102 112-113 -0.1183877215938196 1102 114-115 -0.07048695328012045 1102 116-117 -0.13381481971039833 1102 118-119 -0.10770889300959041 1102 120-121 -0.0857454830806823 1102 122-123 -0.08440885087609828 1102 124-125 -0.019628876173566567 1103 1 0.04263861756325582 1103 2 0.07075638615113178 1103 3 0.057630847100206495 1103 4 0.025574316901021632 1103 5 -0.03545360468675085 1103 6 -0.004772894820419538 1103 7 -0.06089008301576371 1103 8 -0.018797167900707734 1103 9 0.0727966584724058 1103 10-11 0.07413970131240433 1103 12-13 0.08175074900994161 1103 14-15 0.06753850039837772 1103 16-17 0.04068651611962082 1103 18-19 0.03222565122326415 1103 20-21 0.07586795101469335 1103 22-23 -0.019938456415317773 1103 24-25 0.13976392657497172 1103 26-27 0.12240047379535213 1103 28-29 0.12355553574467137 1103 30-31 0.08298002575833863 1103 32-33 0.07103416605253443 1103 34-35 0.12075348737697311 1103 36-37 0.15734645060636865 1103 38-39 0.12102892581410885 1103 40-41 0.19956310999812388 1103 42-43 0.16555289990366617 1103 44-45 0.11406125508949572 1103 46-47 0.10742130324371146 1103 48-49 0.13841374726409583 1103 50-51 0.21131307380498754 1103 52-53 0.14549914504121375 1103 54-55 0.09101050487449669 1103 56-57 0.03984212103252105 1103 58-59 0.12275368599664915 1103 60-61 0.12481905183333453 1103 62-63 0.05915861778969145 1103 64-65 -0.008788181153164487 1103 66-67 0.07922825802199185 1103 68-69 0.1200019644844943 1103 70-71 0.0907637380471158 1103 72-73 0.04306553805152902 1103 74-75 0.021945636370425348 1103 76-77 0.052921489717157044 1103 78-79 -0.041190729915555835 1103 80-81 -0.01578385398557458 1103 82-83 0.014226230511241056 1103 84-85 0.018838812716936104 1103 86-87 0.04512504945152784 1103 88-89 0.04163672951099073 1103 90-91 0.07364815223233734 1103 92-93 0.08647309884943866 1103 94-95 0.08271694781418404 1103 96-97 0.10369133311838397 1103 98-99 0.10898624208389407 1103 100-101 0.10728161522700219 1103 102-103 0.1521600185565788 1103 104-105 0.09707182770490164 1103 106-107 0.14027909121036686 1103 108-109 0.1473124799911858 1103 110-111 0.16214408889477738 1103 112-113 0.22207972875417425 1103 114-115 0.1171333573632296 1103 116-117 0.10683341488474696 1103 118-119 0.020597421707776675 1103 120-121 -0.0219036056405173 1103 122-123 0.1295497300100017 1103 124-125 0.1400507198203229 1104 1 0.006626126535756782 1104 2 0.03316653826569649 1104 3 0.01157120744226603 1104 4 0.061865815725994366 1104 5 0.029570575989026793 1104 6 0.0037644979602831086 1104 7 0.02093130938473564 1104 8 -0.04880505058797269 1104 9 -0.06351954544829397 1104 10-11 0.0017571508943490244 1104 12-13 0.07682734709777606 1104 14-15 0.01071575597771357 1104 16-17 0.039825934800113316 1104 18-19 0.034913917982585474 1104 20-21 0.11783783210246668 1104 22-23 0.06787853085766216 1104 24-25 0.11561858768497046 1104 26-27 0.10657651173713134 1104 28-29 0.1140009526421295 1104 30-31 0.09887548100400778 1104 32-33 0.12331422514809987 1104 34-35 0.13898660597126877 1104 36-37 0.16819917791782757 1104 38-39 0.12866804459194725 1104 40-41 0.06686591580869816 1104 42-43 0.20642621069787026 1104 44-45 0.23283509980874584 1104 46-47 0.2830263937225368 1104 48-49 0.2749691153577345 1104 50-51 0.23364906146801445 1104 52-53 0.3218502024029277 1104 54-55 0.33377053932233736 1104 56-57 0.37528682504662925 1104 58-59 0.30646895136952423 1104 60-61 0.2755479955532465 1104 62-63 0.32292074909788937 1104 64-65 0.2748853149907049 1104 66-67 0.3843942656129684 1104 68-69 0.3412932502638668 1104 70-71 0.30750650023250614 1104 72-73 0.3717770792234063 1104 74-75 0.24390043628783786 1104 76-77 0.22271852690836624 1104 78-79 0.26474674857503544 1104 80-81 0.29569106890831165 1104 82-83 0.3031937050031317 1104 84-85 0.20201050452844527 1104 86-87 0.20673130266769846 1104 88-89 0.30946506146225516 1104 90-91 0.3096926410703915 1104 92-93 0.30839307400504623 1104 94-95 0.28278428251537946 1104 96-97 0.2556536879605886 1104 98-99 0.26327304735037416 1104 100-101 0.31532525434085557 1104 102-103 0.2378132614383972 1104 104-105 0.36762837246578073 1104 106-107 0.2811328591676485 1104 108-109 0.3230726430857871 1104 110-111 0.2823817002930511 1104 112-113 0.3156678960375814 1104 114-115 0.3206147478299357 1104 116-117 0.2544511758410266 1104 118-119 0.3129408495787338 1104 120-121 0.2883643319797322 1104 122-123 0.3285884027031294 1104 124-125 0.1874654524153172 1105 1 0.007114258592970657 1105 2 0.019760402815208522 1105 3 -0.024095079131718933 1105 4 0.020116547484235525 1105 5 -0.10144117495453742 1105 6 -0.00606226305381341 1105 7 -0.0018353496123069135 1105 8 -0.007252833653247137 1105 9 0.043746350072680684 1105 10-11 0.06212933155051559 1105 12-13 0.09376936103642208 1105 14-15 0.09425475835464425 1105 16-17 0.07518823845500577 1105 18-19 0.08162064914184697 1105 20-21 0.01792314280721996 1105 22-23 0.07353359058981823 1105 24-25 0.016857958301535803 1105 26-27 0.07049803996385862 1105 28-29 0.13755894466585517 1105 30-31 0.06869945827742896 1105 32-33 0.014561843378395167 1105 34-35 0.08584511355393687 1105 36-37 0.14189458781485342 1105 38-39 0.08534627564203134 1105 40-41 -0.020528293736994385 1105 42-43 0.046081671961154314 1105 44-45 0.004800504793173843 1105 46-47 0.07399405443616303 1105 48-49 -0.005980499913277981 1105 50-51 -0.005610420502094371 1105 52-53 0.030654310140725727 1105 54-55 -0.019838519876920202 1105 56-57 0.11476857619936709 1105 58-59 0.11901040691792986 1105 60-61 0.23671285809921017 1105 62-63 0.15371447293556173 1105 64-65 0.2124667721499378 1105 66-67 0.13835498711006977 1105 68-69 0.16155489452017235 1105 70-71 0.2618818896189836 1105 72-73 0.18852561927457145 1105 74-75 0.18101399433654564 1105 76-77 0.21701117429463324 1105 78-79 0.19453773930325724 1105 80-81 0.19120959827999684 1105 82-83 0.2530558766062825 1105 84-85 0.25078050753308645 1105 86-87 0.13946173980295384 1105 88-89 0.17370511687121137 1105 90-91 0.23930476082050234 1105 92-93 0.07173533394504261 1105 94-95 0.11195067142701731 1105 96-97 0.18151479533028692 1105 98-99 0.1132483866138898 1105 100-101 0.20782125515722072 1105 102-103 0.13333081337304975 1105 104-105 0.24817686712476927 1105 106-107 0.20777757781142014 1105 108-109 0.22513296307131014 1105 110-111 0.21081952718464692 1105 112-113 0.06873299197596339 1105 114-115 0.17741162018779733 1105 116-117 0.07450179144065316 1105 118-119 0.1604086718626938 1105 120-121 -0.03261318716386086 1105 122-123 0.022138921243250564 1105 124-125 -0.010241910363767204 1106 1 9.337527530774992E-4 1106 2 0.038257800799620156 1106 3 -0.050174061018765315 1106 4 0.024535854268293633 1106 5 0.009422167047461016 1106 6 0.06809098596343688 1106 7 -0.0021596470511298094 1106 8 -2.712000124418523E-4 1106 9 0.003200497148732495 1106 10-11 0.06608519466908547 1106 12-13 0.03570315043502603 1106 14-15 -0.059828664898951445 1106 16-17 -0.01590238702991087 1106 18-19 0.005590151607357541 1106 20-21 0.04024498802947818 1106 22-23 0.09539293487171108 1106 24-25 0.028877514945449434 1106 26-27 -0.0034372510073055196 1106 28-29 0.024093398534830612 1106 30-31 -0.033375800222515295 1106 32-33 -0.01125204420690551 1106 34-35 -2.4794528485472256E-5 1106 36-37 -0.027003289224758475 1106 38-39 -0.11414472821503807 1106 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2216 12-13 -0.09498038535774356 2216 14-15 -0.07235217336883437 2216 16-17 -0.13381807786161204 2216 18-19 -0.12853081516853138 2216 20-21 -0.1710815540176469 2216 22-23 -0.19525557638486646 2216 24-25 -0.14321132128459624 2216 26-27 -0.1754449389866295 2216 28-29 -0.368833491052726 2216 30-31 -0.4251756060017513 2216 32-33 -0.35362714055188604 2216 34-35 -0.30938258489431547 2216 36-37 -0.24007942322697318 2216 38-39 -0.17221655660402746 2216 40-41 -0.18369036930391047 2216 42-43 -0.1642495200773979 2216 44-45 -0.11067889315031465 2216 46-47 -0.04015815209146467 2216 48-49 -0.02633223738588697 2216 50-51 -0.055733690118486834 2216 52-53 -0.04455569341796917 2216 54-55 -0.1455430510773894 2216 56-57 -0.17978208804623108 2216 58-59 -0.19781036046418876 2216 60-61 -0.24693147043770125 2216 62-63 -0.2097937465826547 2216 64-65 -0.18882139608836468 2216 66-67 -0.22255532262818178 2216 68-69 -0.21587968413856373 2216 70-71 -0.17513108633455232 2216 72-73 -0.09581754086775618 2216 74-75 -0.12950263249520333 2216 76-77 -0.1330071570372482 2216 78-79 -0.16852638988078184 2216 80-81 -0.13554893221350994 2216 82-83 -0.031856047842936164 2216 84-85 -0.10479914949919333 2216 86-87 -0.12720826843494137 2216 88-89 -0.09101776627827718 2216 90-91 -0.0890784157079878 2216 92-93 -0.06505845784388953 2216 94-95 -0.034681159235098846 2216 96-97 -0.10123210470255373 2216 98-99 -0.059875131902252576 2216 100-101 -0.044281096123690133 2216 102-103 -0.028344586696690044 2216 104-105 -0.10324933141117043 2216 106-107 -0.11147668508228747 2216 108-109 -0.16533374337549134 2216 110-111 -0.15762418307692627 2216 112-113 -0.15426743620331962 2216 114-115 -0.20147624315578128 2216 116-117 -0.259597488781921 2216 118-119 -0.16782062306976187 2216 120-121 -0.26112847776552073 2216 122-123 -0.1912749823025699 2216 124-125 -0.20239595092097673 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 102.0 3 5.0 4 20.0 5 63.0 6 198.0 7 413.0 8 777.0 9 1652.0 10 3149.0 11 4545.0 12 5572.0 13 9332.0 14 16303.0 15 17702.0 16 6457.0 17 4530.0 18 4103.0 19 3351.0 20 3155.0 21 2992.0 22 2998.0 23 2892.0 24 3340.0 25 4413.0 26 5725.0 27 7301.0 28 9365.0 29 11902.0 30 15448.0 31 19826.0 32 25724.0 33 33783.0 34 49194.0 35 84641.0 36 209439.0 37 618399.0 38 373157.0 39 1631.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.68403243520539 19.1409160892256 14.58381706615802 22.591234409410994 2 15.067290270715189 20.88949916186951 39.99938603184064 24.04382453557466 3 18.660666833376077 26.072541617128174 29.804572655776834 25.462218893718912 4 12.353039366231368 17.349397128036024 37.84806718346584 32.44949632226677 5 13.030134964987491 38.19431013127609 33.91730208358684 14.858252820149579 6 30.49726944056628 37.407545029128315 17.39972972609985 14.695455804205555 7 27.20608033133815 33.81001139038845 21.321259478932898 17.662648799340495 8 26.16099140508532 34.19514850035079 20.07119472447859 19.572665370085296 9 26.54548896488166 16.596007032493628 20.662458852941196 36.19604514968352 10-11 24.681967691204715 26.89868054405253 27.19079508237086 21.228556682371888 12-13 26.376743653583816 24.31656070386333 27.89625089297192 21.410444749580932 14-15 22.765043978667165 25.31278799743413 27.065251384786 24.856916639112715 16-17 21.430757320373484 28.658092214849272 27.574470044579858 22.336680420197386 18-19 21.379554476563364 26.75433407158741 30.634037243564364 21.23207420828486 20-21 22.401978592114286 25.653998372918817 30.549440498971563 21.394582535995333 22-23 22.0538745302495 25.634371026786972 30.57477762798063 21.736976814982896 24-25 22.017825542226618 25.87300196533766 30.63800245459354 21.47117003784218 26-27 21.673939417970974 25.88803139423855 30.903543683514762 21.534485504275715 28-29 21.413706455427512 26.34994650162861 30.67560800435406 21.56073903858982 30-31 21.644488132826893 25.875208413410345 30.687887367541165 21.792416086221593 32-33 21.678704066707642 25.92007285755491 30.495318812559997 21.90590426317745 34-35 22.224720020926082 25.821326311925247 30.288488288877137 21.665465378271538 36-37 22.11858667087917 25.572221522270095 30.38458070131792 21.92461110553281 38-39 21.930942652176167 25.97616140711269 30.169468002985422 21.92342793772572 40-41 22.089327250784887 25.826346780728304 30.12358027857526 21.960745689911544 42-43 22.056198552186334 25.996307237341544 29.694921779816948 22.252572430655174 44-45 22.13096196659118 25.934750533864502 29.55546786612169 22.378819633422637 46-47 22.575417354449577 26.428579194537733 28.800223075097897 22.195780375914797 48-49 22.70240003990793 26.567937175708096 28.20966245181789 22.520000332566088 50-51 22.477662111577203 26.73729005966364 28.29680116193474 22.48824666682442 52-53 22.84767386011375 26.881380712062363 27.82919405806732 22.441751369756567 54-55 22.38812508833787 26.621723344668297 27.964554850700214 23.025596716293627 56-57 22.495793358783168 26.89887240910233 27.8716282115811 22.733706020533397 58-59 22.800315170321802 26.4327042931084 27.934432037881834 22.832548498687963 60-61 22.49959868227084 26.551884466541615 28.50922774957006 22.439289101617486 62-63 22.27185486815993 26.64644195858401 28.518117496877398 22.56358567637866 64-65 22.179887554289817 26.710524885216735 28.22709019384126 22.882497366652192 66-67 22.27885794247758 26.761337145905056 27.920681709312937 23.039123202304427 68-69 22.207963806577006 26.532602029036855 28.331592690964886 22.927841473421253 70-71 22.514564156155124 26.597900100984972 27.97459578830634 22.912939954553565 72-73 22.552297615948845 26.454928661376737 28.06883350526574 22.923940217408685 74-75 22.27067170035284 27.17128880230801 27.70301081031646 22.855028687022696 76-77 22.551178403158353 26.655043908316646 27.7508811402412 23.0428965482838 78-79 22.482011052705968 26.79839907802448 27.783466221198655 22.936123648070893 80-81 22.516131054061812 26.96221985304416 27.57180709376253 22.94984199913149 82-83 22.472769552807335 26.72187690066315 27.860627948725984 22.94472559780353 84-85 22.330725460939792 26.79002097084994 27.749474129876013 23.129779438334253 86-87 22.45745232633175 26.735947004315047 27.55585031712095 23.250750352232256 88-89 22.35269400914173 26.866031508078482 27.252000033256607 23.529274449523182 90-91 22.36296675054418 26.711710458531474 27.592951986438898 23.332370804485446 92-93 22.489205992073416 26.719254744982567 27.569408780640046 23.222130482303964 94-95 22.29916432456044 26.532351444804263 27.627366715795205 23.54111751484009 96-97 22.303992264001195 26.514950444455387 27.657954501122088 23.52310279042133 98-99 22.306704191626725 26.604209630262314 27.204178355909175 23.884907822201782 100-101 22.094763427195847 26.40047096474224 27.587572005354318 23.9171936027076 102-103 22.437669321231315 26.241497164229123 27.404297529540877 23.91653598499868 104-105 22.455790947037396 26.31050633951696 27.49200465218758 23.741698061258067 106-107 22.32620239623791 26.42196876544019 27.478353749698194 23.773475088623705 108-109 22.221444101111828 26.460520000217446 27.165401793494542 24.15263410517618 110-111 22.007717192338834 26.830834487778233 27.229197926220415 23.932250393662514 112-113 22.117852936313593 26.563937611670994 27.161616294059577 24.156593157955836 114-115 21.677549705999642 26.796580916695262 27.134120894636037 24.39174848266906 116-117 21.91547514015153 26.62739982815029 27.11922129759644 24.337903734101744 118-119 21.829321055655985 26.76352243049917 26.985958333879616 24.421198179965227 120-121 21.57300776641966 27.14451130267164 27.060026266468107 24.222454664440594 122-123 21.554629977535637 27.0044688896528 26.95784533964361 24.483055793167953 124-125 21.56856161409177 27.047881814734016 26.85025828563827 24.53329828553594 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 213.0 1 168.0 2 203.5 3 264.0 4 173.0 5 131.5 6 324.0 7 878.0 8 1593.5 9 1833.0 10 1456.0 11 1127.0 12 1192.0 13 1370.5 14 1566.0 15 1799.5 16 2033.5 17 2303.5 18 2551.5 19 2779.5 20 3013.0 21 3394.0 22 3894.0 23 4543.0 24 5324.5 25 6492.5 26 8182.5 27 10124.5 28 12471.5 29 14948.5 30 17832.5 31 21473.0 32 25867.0 33 29246.5 34 31163.5 35 32741.0 36 36111.0 37 39440.5 38 40925.0 39 42790.0 40 44914.5 41 47891.0 42 50242.5 43 52188.5 44 55224.0 45 58255.5 46 59356.0 47 61625.5 48 67811.0 49 66715.0 50 60325.0 51 56357.0 52 53266.5 53 52058.0 54 50960.0 55 47849.5 56 42654.0 57 38060.5 58 35192.0 59 31548.0 60 26670.0 61 21802.5 62 17905.5 63 13890.5 64 10042.0 65 7664.0 66 5514.0 67 4326.5 68 3083.5 69 1813.5 70 1287.0 71 919.0 72 656.5 73 519.5 74 431.0 75 349.0 76 224.5 77 87.0 78 37.5 79 18.5 80 7.5 81 3.5 82 3.5 83 2.0 84 1.0 85 1.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 1.0 98 4.0 99 5.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015093383917487796 2 0.0 3 0.0 4 0.0 5 0.13795097080517446 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.1977508299762275E-5 18-19 0.0 20-21 0.004029166045770047 22-23 1.9186504979857365E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 6.075726576954833E-4 92-93 0.0 94-95 8.633927240935816E-4 96-97 0.0 98-99 0.0011192127904916798 100-101 0.0 102-103 1.9186504979857365E-4 104-105 0.0021424930560840724 106-107 0.0068112092678493654 108-109 2.2384255809833597E-4 110-111 0.004924536278163391 112-113 0.008537994716036529 114-115 0.005148378836261727 116-117 0.0015029428900888272 118-119 1.598875414988114E-4 120-121 5.436176410959588E-4 122-123 7.354826908945324E-4 124-125 0.0012791003319904911 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1563599.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.55606213330347 #Duplication Level Percentage of deduplicated Percentage of total 1 65.49239894974843 26.561138010650964 2 15.25707853861509 12.37534050369529 3 6.6131615655293645 8.046113740475501 4 3.6518972121693847 5.924262809647172 5 2.2382029762134046 4.5386349485127795 6 1.4772062738199208 3.594580165484785 7 0.997538858905699 2.8319373559514927 8 0.7335106915576279 2.3798644145802847 9 0.5530817747480206 2.018773693933063 >10 2.7639819411796696 19.54616775708603 >50 0.14887178417914718 4.109281869292596 >100 0.06675683250381073 4.920641444822505 >500 0.004892265643613622 1.4133905685715762 >1k 0.0011047051453321084 0.9076984577947785 >5k 3.1563004152345954E-4 0.8321742595013193 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7397 0.47307525778668313 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5605 0.35846786804033515 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3633 0.23234857530607272 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3365 0.21520863085740016 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1934 0.1236890021034805 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 6.395501659952456E-5 0.0 6 0.0 0.0 0.0 6.395501659952456E-5 0.0 7 0.0 0.0 0.0 6.395501659952456E-5 0.0 8 0.0 0.0 0.0 6.395501659952456E-5 0.0 9 0.0 0.0 0.0 6.395501659952456E-5 0.0 10-11 0.0 0.0 0.0 6.395501659952456E-5 0.0 12-13 0.0 0.0 0.0 6.395501659952456E-5 0.0 14-15 0.0 0.0 0.0 6.395501659952456E-5 0.0 16-17 0.0 0.0 0.0 6.395501659952456E-5 0.0 18-19 0.0 0.0 0.0 6.395501659952456E-5 0.0 20-21 0.0 0.0 0.0 9.593252489928684E-5 0.0 22-23 0.0 0.0 0.0 1.598875414988114E-4 0.0 24-25 0.0 0.0 0.0 3.197750829976228E-4 0.0 26-27 0.0 0.0 0.0 7.674601991942947E-4 0.0 28-29 0.0 0.0 0.0 0.0026861106971800314 0.0 30-31 0.0 0.0 0.0 0.007834489533441758 0.0 32-33 0.0 0.0 0.0 0.019090572454958078 0.0 34-35 0.0 0.0 0.0 0.035558989229335654 0.0 36-37 0.0 0.0 0.0 0.06286778131733264 0.0 38-39 0.0 0.0 0.0 0.10590950748881267 0.0 40-41 0.0 0.0 0.0 0.15867239618342044 0.0 42-43 0.0 0.0 0.0 0.21159517241952702 0.0 44-45 0.0 0.0 0.0 0.27174486553137983 0.0 46-47 0.0 0.0 0.0 0.34414194432204165 0.0 48-49 0.0 0.0 0.0 0.4255566804532364 0.0 50-51 0.0 0.0 0.0 0.5126953905700886 0.0 52-53 0.0 0.0 0.0 0.5988427979296482 0.0 54-55 0.0 0.0 0.0 0.6885397087104814 0.0 56-57 0.0 0.0 0.0 0.7979347646039682 0.0 58-59 0.0 0.0 0.0 0.9174347131201798 0.0 60-61 0.0 0.0 0.0 1.0455046338607277 0.0 62-63 0.0 0.0 0.0 1.1769321929727508 0.0 64-65 0.0 0.0 0.0 1.3163221516514145 0.0 66-67 0.0 0.0 0.0 1.4739392900609427 0.0 68-69 0.0 0.0 0.0 1.6565308624525854 0.0 70-71 0.0 0.0 0.0 1.8565501768675985 0.0 72-73 0.0 0.0 0.0 2.0546508407846256 0.0 74-75 0.0 0.0 0.0 2.2650628453970616 0.0 76-77 0.0 0.0 0.0 2.496899780570338 0.0 78-79 0.0 0.0 0.0 2.760074673877382 0.0 80-81 0.0 0.0 0.0 3.0449942728282635 0.0 82-83 0.0 0.0 0.0 3.332280207393328 0.0 84-85 0.0 0.0 0.0 3.6229877353464666 0.0 86-87 0.0 0.0 0.0 3.9323381506383672 0.0 88-89 0.0 0.0 0.0 4.274337601904325 0.0 90-91 0.0 0.0 0.0 4.647355236221052 0.0 92-93 0.0 0.0 0.0 5.040902430866226 0.0 94-95 0.0 0.0 0.0 5.427446551193752 0.0 96-97 0.0 0.0 0.0 5.828156707698073 0.0 98-99 0.0 0.0 0.0 6.26036470987766 0.0 100-101 0.0 0.0 0.0 6.74856532909013 0.0 102-103 0.0 0.0 0.0 7.258414721421541 0.0 104-105 0.0 0.0 0.0 7.754737627742151 0.0 106-107 0.0 0.0 0.0 8.261517179276783 0.0 108-109 0.0 0.0 0.0 8.800785879243975 0.0 110-111 0.0 0.0 0.0 9.389715649600697 0.0 112-113 0.0 0.0 0.0 10.005602459454117 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14945 0.0 32.73151 1 GTACATG 7290 0.0 30.731173 1 TACATGG 7455 0.0 29.887331 2 GAGTACT 8440 0.0 29.60645 12-13 ACATGGG 7210 0.0 28.837217 3 ATCAACG 17190 0.0 28.279877 3 CAACGCA 17270 0.0 28.183369 5 TCAACGC 17290 0.0 28.15077 4 TATCAAC 17385 0.0 28.134012 2 AACGCAG 17490 0.0 27.825502 6 AGTACTT 8680 0.0 27.69116 12-13 GGTATCA 11035 0.0 26.889072 1 GTACTTT 9230 0.0 26.782787 14-15 AGAGTAC 13175 0.0 26.304182 10-11 TATATCG 115 9.976265E-4 25.902122 5 ACGCAGA 18985 0.0 25.446318 7 CGCAGAG 19150 0.0 25.196 8 GCAGAGT 19970 0.0 23.833696 9 TACTTTT 10290 0.0 23.185444 14-15 ATACGGC 130 0.002053518 22.88264 6 >>END_MODULE