FastQCFastQC Report
Fri 27 May 2016
SRR939967_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939967_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1675209
Sequences flagged as poor quality0
Sequence length125
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40490.24170118474769417No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21500.12834219491418683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC7250.01749.1663108-109
CGTCGCT953.6379788E-111304.7584110-111
CCGCGCC6650.01046.9727108-109
CCTCGCC6500.0994.62384108-109
CCGTTCG859.199903E-8877.6094108-109
TTGTTCG2300.0864.8904108-109
CCACGCG809.024813E-5621.63995108-109
GTCGCGC501.216959E-5619.7603110-111
CTCCGCC4700.0593.38745110-111
CGTCGCC3000.0580.19727108-109
CCGCGCA951.5105796E-4523.48627108-109
TTTTTCG9450.0412.544292-93
CCGCTCG2551.5090336E-8390.0486108-109
CCGGTCG1303.8667573E-4382.54764108-109
CGCCGCC5700.0380.55453110-111
CAGCGCG2651.8284481E-8375.3298108-109
CGGGTCG850.003216004364.56488110-111
AAGCGCG2053.1006966E-6363.88678108-109
CCGCGCG4301.8189894E-12346.96182108-109
CGTGTCT3602.8003342E-8344.31125110-111
Produced by FastQC (version 0.11.5)