##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR939967_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1675209
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.848549046715963	34.0	31.0	34.0	30.0	34.0
2	31.908368448354803	34.0	31.0	34.0	30.0	34.0
3	32.02901906568076	34.0	31.0	34.0	30.0	34.0
4	35.31007772761488	37.0	35.0	37.0	33.0	37.0
5	35.31887603278158	37.0	35.0	37.0	33.0	37.0
6	35.44749341723928	37.0	37.0	37.0	35.0	37.0
7	35.36669752848749	37.0	36.0	37.0	33.0	37.0
8	35.36491207962708	37.0	37.0	37.0	33.0	37.0
9	37.03069646832127	39.0	38.0	39.0	34.0	39.0
10-11	37.00825389548409	39.0	38.0	39.0	34.0	39.0
12-13	36.94872968089355	39.0	38.0	39.0	34.0	39.0
14-15	38.444966269880354	41.0	39.0	41.0	34.0	41.0
16-17	38.441298369337794	41.0	39.0	41.0	34.0	41.0
18-19	38.45652751388036	41.0	39.0	41.0	34.0	41.0
20-21	38.520170319046755	41.0	39.0	41.0	34.0	41.0
22-23	38.50330705004569	41.0	39.0	41.0	34.0	41.0
24-25	38.520803971325364	41.0	39.0	41.0	34.0	41.0
26-27	38.493754212160994	41.0	39.0	41.0	34.0	41.0
28-29	38.44487135635016	41.0	39.0	41.0	34.0	41.0
30-31	38.37634229520018	41.0	39.0	41.0	34.0	41.0
32-33	38.27501702772609	40.5	38.5	41.0	33.0	41.0
34-35	38.18242589432124	40.0	38.0	41.0	33.0	41.0
36-37	38.34230594510894	41.0	39.0	41.0	33.0	41.0
38-39	38.31974786429633	41.0	39.0	41.0	33.0	41.0
40-41	38.27508895904929	41.0	39.0	41.0	33.0	41.0
42-43	38.15657777626553	41.0	38.5	41.0	33.0	41.0
44-45	38.02053266189473	40.5	38.0	41.0	33.0	41.0
46-47	37.916861418485695	40.0	38.0	41.0	33.0	41.0
48-49	37.79376901628394	40.0	38.0	41.0	33.0	41.0
50-51	37.65016484510291	40.0	38.0	41.0	32.5	41.0
52-53	37.50377117123893	40.0	37.0	41.0	32.0	41.0
54-55	37.324128511726	40.0	37.0	41.0	32.0	41.0
56-57	37.123358936108865	40.0	36.5	41.0	31.5	41.0
58-59	36.91838451202208	40.0	36.0	41.0	31.0	41.0
60-61	36.65577369749088	39.0	35.5	41.0	31.0	41.0
62-63	36.38322860013288	39.0	35.0	41.0	31.0	41.0
64-65	36.06702715899927	39.0	35.0	41.0	31.0	41.0
66-67	35.73391827527192	38.0	35.0	40.5	30.0	41.0
68-69	35.35775834537661	37.0	35.0	40.0	30.0	41.0
70-71	34.96974019361166	37.0	35.0	39.5	30.0	41.0
72-73	34.58038787996006	36.0	34.5	39.0	29.5	41.0
74-75	34.17550138520029	36.0	34.0	39.0	29.0	40.5
76-77	33.76999735555384	35.0	34.0	37.0	29.0	39.5
78-79	33.3587847844657	35.0	34.0	37.0	29.0	39.0
80-81	32.96386540425702	35.0	34.0	36.5	28.0	39.0
82-83	32.82031943476903	35.0	34.0	36.0	29.0	38.0
84-85	32.47811079095206	35.0	34.0	36.0	29.0	37.0
86-87	31.8943552714915	35.0	34.0	35.5	26.5	37.0
88-89	16.523221878583506	18.0	16.5	18.5	2.0	19.0
90-91	2.774904205982656	2.0	2.0	2.0	2.0	2.0
92-93	2.293972274504256	2.0	2.0	2.0	2.0	2.0
94-95	2.1022681349013768	2.0	2.0	2.0	2.0	2.0
96-97	2.0000083571661804	2.0	2.0	2.0	2.0	2.0
98-99	2.0	2.0	2.0	2.0	2.0	2.0
100-101	2.0	2.0	2.0	2.0	2.0	2.0
102-103	2.0	2.0	2.0	2.0	2.0	2.0
104-105	2.0	2.0	2.0	2.0	2.0	2.0
106-107	2.0	2.0	2.0	2.0	2.0	2.0
108-109	2.0	2.0	2.0	2.0	2.0	2.0
110-111	2.0	2.0	2.0	2.0	2.0	2.0
112-113	2.0	2.0	2.0	2.0	2.0	2.0
114-115	2.0	2.0	2.0	2.0	2.0	2.0
116-117	2.0	2.0	2.0	2.0	2.0	2.0
118-119	2.0	2.0	2.0	2.0	2.0	2.0
120-121	2.0	2.0	2.0	2.0	2.0	2.0
122-123	2.0	2.0	2.0	2.0	2.0	2.0
124-125	2.0	2.0	2.0	2.0	2.0	2.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.19136247700468445
1101	2	0.2930901208255676
1101	3	0.2940218271337969
1101	4	0.35374887591889603
1101	5	0.24664125406633275
1101	6	0.31707682524172043
1101	7	0.3539455493378725
1101	8	0.43009276188927714
1101	9	0.4537939620216136
1101	10-11	0.4099931288680452
1101	12-13	0.4829030694235996
1101	14-15	0.442727783076343
1101	16-17	0.5313986214885631
1101	18-19	0.43255829698013315
1101	20-21	0.40054267463757043
1101	22-23	0.4522732126852702
1101	24-25	0.4452167863212111
1101	26-27	0.4252043071522422
1101	28-29	0.453892464468062
1101	30-31	0.38274004336506806
1101	32-33	0.5029978385626563
1101	34-35	0.5739749914930243
1101	36-37	0.4293079699694573
1101	38-39	0.44554497926578307
1101	40-41	0.47246922214582554
1101	42-43	0.47123591000475784
1101	44-45	0.43408698441496085
1101	46-47	0.3863942060278589
1101	48-49	0.4496874387304288
1101	50-51	0.34518518237698714
1101	52-53	0.29062363878469455
1101	54-55	0.3960430459074544
1101	56-57	0.36984757779212174
1101	58-59	0.33997403973705786
1101	60-61	0.1705524093918811
1101	62-63	0.24216648679612263
1101	64-65	0.307896627265805
1101	66-67	0.34450630006248417
1101	68-69	0.27775743055868674
1101	70-71	0.2013768529803741
1101	72-73	0.22832434661870593
1101	74-75	0.3107356889910946
1101	76-77	0.29126794083747143
1101	78-79	0.35093810296947225
1101	80-81	0.4006701409485913
1101	82-83	0.42144827102492854
1101	84-85	0.49984170879439915
1101	86-87	0.5625176790180113
1101	88-89	1.0453954279341815
1101	90-91	-0.059369470073530994
1101	92-93	-0.22545983457162944
1101	94-95	-0.03209881799919323
1101	96-97	0.0
1101	98-99	0.0
1101	100-101	0.0
1101	102-103	0.0
1101	104-105	0.0
1101	106-107	0.0
1101	108-109	0.0
1101	110-111	0.0
1101	112-113	0.0
1101	114-115	0.0
1101	116-117	0.0
1101	118-119	0.0
1101	120-121	0.0
1101	122-123	0.0
1101	124-125	0.0
1102	1	-0.04561860871220702
1102	2	-0.005299889588783913
1102	3	0.07918933622425328
1102	4	0.05141258084007205
1102	5	0.04838498288746251
1102	6	-0.008798505965309289
1102	7	0.030109159794662332
1102	8	0.03078370140507758
1102	9	0.13806457131225613
1102	10-11	0.15762523708048803
1102	12-13	0.07399391438194414
1102	14-15	0.041463728590400706
1102	16-17	0.06028238748120174
1102	18-19	0.041500770495197514
1102	20-21	0.003939949037530255
1102	22-23	0.0061747780020340315
1102	24-25	0.11950413861008258
1102	26-27	0.07628236451772352
1102	28-29	0.14961960292307452
1102	30-31	0.1435546335059641
1102	32-33	0.1167404058906385
1102	34-35	0.0696097130650557
1102	36-37	0.12893067083192022
1102	38-39	0.08321123426006949
1102	40-41	0.03774923227166482
1102	42-43	-0.05123777258932449
1102	44-45	-0.027310865147484265
1102	46-47	0.0070617109302375525
1102	48-49	-0.061708316793875895
1102	50-51	-0.09165658000273424
1102	52-53	-0.13361275857626254
1102	54-55	-0.11411285290489559
1102	56-57	-0.2401565265226182
1102	58-59	-0.18926040784172926
1102	60-61	-0.17879727543211743
1102	62-63	-0.16765901933289484
1102	64-65	-0.1842442149470287
1102	66-67	-0.08174995994818346
1102	68-69	-0.08109136965052954
1102	70-71	-0.12243003389224327
1102	72-73	-0.12130026214516931
1102	74-75	-0.04274148459722937
1102	76-77	-0.07537151884485382
1102	78-79	-0.08439129853581306
1102	80-81	-0.16106210323673054
1102	82-83	-0.07837759807070199
1102	84-85	-0.11526310014090768
1102	86-87	0.09838315428556044
1102	88-89	1.0785420040887637
1102	90-91	0.1560509336756346
1102	92-93	-0.005621941043696399
1102	94-95	0.12377896090574625
1102	96-97	0.0
1102	98-99	0.0
1102	100-101	0.0
1102	102-103	0.0
1102	104-105	0.0
1102	106-107	0.0
1102	108-109	0.0
1102	110-111	0.0
1102	112-113	0.0
1102	114-115	0.0
1102	116-117	0.0
1102	118-119	0.0
1102	120-121	0.0
1102	122-123	0.0
1102	124-125	0.0
1103	1	-0.10996241576760823
1103	2	0.048721279161135556
1103	3	-0.04584417883518199
1103	4	-0.09340439501631437
1103	5	-0.15670930614641065
1103	6	-0.17344490612873642
1103	7	-0.09276917734768375
1103	8	-0.12802788650651564
1103	9	-0.12280771396793
1103	10-11	-0.16955196501851333
1103	12-13	-0.1670068133736322
1103	14-15	-0.15816026436186092
1103	16-17	-0.10033454496520733
1103	18-19	-0.07294581607579431
1103	20-21	-0.06143867537675618
1103	22-23	-0.049427663171044856
1103	24-25	-0.12667450623242615
1103	26-27	-0.09480275734941301
1103	28-29	-0.16511106367549644
1103	30-31	-0.2117956467437736
1103	32-33	-0.09742720770945823
1103	34-35	-0.08829664905079682
1103	36-37	-0.08478752178071858
1103	38-39	-0.12578548445601712
1103	40-41	-0.12616813555132467
1103	42-43	-0.13024713127068566
1103	44-45	-0.20034624428259917
1103	46-47	-0.2053043884275496
1103	48-49	-0.33047036625571735
1103	50-51	-0.4058446218295302
1103	52-53	-0.3953117486558
1103	54-55	-0.37736119630339005
1103	56-57	-0.31167510982509583
1103	58-59	-0.20586344909397525
1103	60-61	-0.24066543676403995
1103	62-63	-0.39136342289251047
1103	64-65	-0.28062714205655936
1103	66-67	-0.22953477411515877
1103	68-69	-0.18271860558377995
1103	70-71	-0.12205466522659236
1103	72-73	-0.0908577356842386
1103	74-75	0.005968345210106918
1103	76-77	0.01714025930461105
1103	78-79	-0.08127152528913228
1103	80-81	-0.047503506145197605
1103	82-83	-0.05525156867958714
1103	84-85	-0.10564395889248601
1103	86-87	-0.1815939916358147
1103	88-89	0.6363781441753282
1103	90-91	-0.47307333378092364
1103	92-93	-0.3269439026641656
1103	94-95	-0.11481378962730293
1103	96-97	0.0
1103	98-99	0.0
1103	100-101	0.0
1103	102-103	0.0
1103	104-105	0.0
1103	106-107	0.0
1103	108-109	0.0
1103	110-111	0.0
1103	112-113	0.0
1103	114-115	0.0
1103	116-117	0.0
1103	118-119	0.0
1103	120-121	0.0
1103	122-123	0.0
1103	124-125	0.0
1104	1	-0.0822411957233804
1104	2	-0.04604008536558979
1104	3	0.07734339475397434
1104	4	0.14723477833084075
1104	5	0.10218677514503582
1104	6	0.14298443272000583
1104	7	0.13683792856953403
1104	8	0.16431232999541123
1104	9	0.1626981697655907
1104	10-11	0.18545302768473704
1104	12-13	0.17237751172057614
1104	14-15	0.19054428030396053
1104	16-17	0.19397001673054604
1104	18-19	0.1757543101102641
1104	20-21	0.11270139365171161
1104	22-23	-0.08960515010433312
1104	24-25	0.0357986295894932
1104	26-27	0.13470757133896427
1104	28-29	0.11267473414989126
1104	30-31	0.054572853037448965
1104	32-33	0.09217199503221707
1104	34-35	0.14157681785300014
1104	36-37	-0.017393988360012713
1104	38-39	0.028911126981434165
1104	40-41	0.15616860698797552
1104	42-43	0.08569229298172587
1104	44-45	0.06088349197887766
1104	46-47	0.047155470488782214
1104	48-49	0.10653096715918764
1104	50-51	0.015488997005576266
1104	52-53	0.008629159633372296
1104	54-55	0.005701500672259385
1104	56-57	-0.01883964792938997
1104	58-59	-0.08514235319650965
1104	60-61	-0.06003064969718963
1104	62-63	-0.02722398354214306
1104	64-65	-0.0746492430418968
1104	66-67	0.013426068852069761
1104	68-69	0.04753418870217985
1104	70-71	0.026200536146966158
1104	72-73	0.013638326859975791
1104	74-75	-0.0031373008199366836
1104	76-77	-0.006987502379409705
1104	78-79	-0.056908797080019724
1104	80-81	-0.03376927895519799
1104	82-83	-0.0021786501681617665
1104	84-85	0.050834802530715706
1104	86-87	-0.10924174327360348
1104	88-89	0.6112742025693834
1104	90-91	-0.6356347497552126
1104	92-93	-0.3269439026641656
1104	94-95	-0.11481378962730293
1104	96-97	0.0
1104	98-99	0.0
1104	100-101	0.0
1104	102-103	0.0
1104	104-105	0.0
1104	106-107	0.0
1104	108-109	0.0
1104	110-111	0.0
1104	112-113	0.0
1104	114-115	0.0
1104	116-117	0.0
1104	118-119	0.0
1104	120-121	0.0
1104	122-123	0.0
1104	124-125	0.0
1105	1	-0.17478472554544666
1105	2	-0.2243241841235566
1105	3	-0.17718294383658773
1105	4	-0.08105531467536764
1105	5	-0.13869856169765882
1105	6	-0.09286641676033724
1105	7	-0.0891823537868035
1105	8	-0.11530368391124313
1105	9	-0.02485691941928536
1105	10-11	-0.16354896404047992
1105	12-13	-0.03212946476698164
1105	14-15	-0.18206934475853842
1105	16-17	-0.10406817821058212
1105	18-19	-0.07119994764513393
1105	20-21	-0.015724438424875586
1105	22-23	-0.14835440252621623
1105	24-25	-0.04613746491255455
1105	26-27	0.0032058554854046406
1105	28-29	-0.019338451152748348
1105	30-31	-0.09973910603273595
1105	32-33	-0.09743765679732519
1105	34-35	0.07295680959728656
1105	36-37	-0.05439774293914468
1105	38-39	-0.08349941908095815
1105	40-41	-0.02978174360742969
1105	42-43	0.04635708235883129
1105	44-45	-0.03284858487828757
1105	46-47	0.006295387822646603
1105	48-49	-0.028082819339317666
1105	50-51	-0.09544018640071528
1105	52-53	-0.05359888541067903
1105	54-55	-0.15510961988433536
1105	56-57	-0.20014607389973804
1105	58-59	-0.3599335081382833
1105	60-61	-0.4386331588350245
1105	62-63	-0.35921210198848996
1105	64-65	-0.4440682914004981
1105	66-67	-0.3048875004911622
1105	68-69	-0.3009845167581773
1105	70-71	-0.28028920823921766
1105	72-73	-0.2391099167571724
1105	74-75	-0.31737954950767033
1105	76-77	-0.20973469019146052
1105	78-79	-0.16641417623665689
1105	80-81	-0.30406466256665254
1105	82-83	-0.17889649613505298
1105	84-85	-0.14294110726675058
1105	86-87	-0.11787388647619323
1105	88-89	0.9700601867908887
1105	90-91	-0.7878723072625315
1105	92-93	-0.3269439026641656
1105	94-95	-0.11481378962730293
1105	96-97	0.0
1105	98-99	0.0
1105	100-101	0.0
1105	102-103	0.0
1105	104-105	0.0
1105	106-107	0.0
1105	108-109	0.0
1105	110-111	0.0
1105	112-113	0.0
1105	114-115	0.0
1105	116-117	0.0
1105	118-119	0.0
1105	120-121	0.0
1105	122-123	0.0
1105	124-125	0.0
1106	1	-0.057980194145606845
1106	2	-0.04244424090051879
1106	3	0.02512532568606929
1106	4	0.048727370449931584
1106	5	-0.036222243909335816
1106	6	0.032578465113935806
1106	7	-0.08766552469096922
1106	8	-0.042594932151722276
1106	9	-0.030402282612122633
1106	10-11	-0.11619323170555163
1106	12-13	-0.08319273736593402
1106	14-15	-0.051224633293863064
1106	16-17	-0.14513792480009613
1106	18-19	-0.06482196826483033
1106	20-21	-0.006673888168471365
1106	22-23	-0.07921766030123933
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2215	122-123	0.0
2215	124-125	0.0
2216	1	0.13064325970414714
2216	2	-0.022217181022877952
2216	3	0.03389777087507184
2216	4	-0.025647955346762785
2216	5	0.06689304715551003
2216	6	0.009801846370024236
2216	7	0.03437396228794398
2216	8	0.01783794149488216
2216	9	-0.1336517338016634
2216	10-11	-0.04810503155982104
2216	12-13	0.030724961660098415
2216	14-15	-0.047531227012207466
2216	16-17	-0.09474310795190632
2216	18-19	-0.12011621214985979
2216	20-21	-0.08439224151887004
2216	22-23	-0.06858156440993213
2216	24-25	-0.10967509233636008
2216	26-27	-0.1259028801926334
2216	28-29	-0.12913773483279556
2216	30-31	-0.16539826608256902
2216	32-33	-0.3101462527412764
2216	34-35	-0.17972625720040725
2216	36-37	-0.23283793768960948
2216	38-39	-0.22838267636816312
2216	40-41	-0.2746981720751762
2216	42-43	-0.2744877344777663
2216	44-45	-0.2672046723447252
2216	46-47	-0.2788934124589062
2216	48-49	-0.20380835569158506
2216	50-51	-0.2187371119374788
2216	52-53	-0.21417278991319222
2216	54-55	-0.17732628239100734
2216	56-57	-0.23436774041149278
2216	58-59	-0.11872210863560895
2216	60-61	-0.15913130354648075
2216	62-63	-0.19236139031589516
2216	64-65	-0.1923419801401991
2216	66-67	-0.22659156969817218
2216	68-69	-0.2555352998643414
2216	70-71	-0.2528872153052859
2216	72-73	-0.23512617677118897
2216	74-75	-0.21994537491524824
2216	76-77	-0.24992031564772077
2216	78-79	-0.20345780815325298
2216	80-81	-0.21080332065989893
2216	82-83	-0.13014053942129067
2216	84-85	-0.2171461868828004
2216	86-87	-0.2667749556821626
2216	88-89	-1.076062179226783
2216	90-91	-0.7878723072625315
2216	92-93	-0.3269439026641656
2216	94-95	-0.11481378962730293
2216	96-97	0.0
2216	98-99	0.0
2216	100-101	0.0
2216	102-103	0.0
2216	104-105	0.0
2216	106-107	0.0
2216	108-109	0.0
2216	110-111	0.0
2216	112-113	0.0
2216	114-115	0.0
2216	116-117	0.0
2216	118-119	0.0
2216	120-121	0.0
2216	122-123	0.0
2216	124-125	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	17331.0
3	3127.0
4	2147.0
5	1872.0
6	2139.0
7	2267.0
8	2477.0
9	2939.0
10	3553.0
11	4363.0
12	5054.0
13	5853.0
14	6244.0
15	7053.0
16	7876.0
17	9287.0
18	11470.0
19	14414.0
20	16120.0
21	22559.0
22	26194.0
23	34364.0
24	53358.0
25	90796.0
26	197272.0
27	551407.0
28	553743.0
29	19288.0
30	642.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.96120663153075	19.211155145417678	14.229030526937237	23.598607696114335
2	14.793289366233841	20.358621578899612	40.33597342871742	24.51211562614912
3	19.043882326734952	25.36451157688171	28.798191222340268	26.793414874043076
4	12.4058401362149	16.842678598410306	38.07262604865108	32.67885521672371
5	13.695867308575526	38.25186740652217	34.110814683157535	13.941450601744766
6	31.4449122467704	36.718761658992996	18.048434553539288	13.787891540697311
7	28.382906252294486	32.27459976635751	21.559518842126565	17.782975139221435
8	25.82424043805877	35.10893267646007	20.17694508565797	18.889881799823186
9	25.90226055375777	17.516978478506264	20.489562794851267	36.091198172884695
10-11	24.70825819291701	26.9562326102542	27.52518062054791	20.810328576280877
12-13	25.61740472278311	24.171826567632383	27.620893905272727	22.589874804311783
14-15	22.531202095500976	25.70967306746386	25.52909847612953	26.23002636090563
16-17	22.489081926738475	28.795866055716203	26.219463420574453	22.495588596970876
18-19	22.52325934381025	28.148067002193027	27.15880052260206	22.16987313139466
20-21	23.373813814064786	27.168952156781746	26.85859550332071	22.598638525832758
22-23	23.040126026968768	27.094485006838003	26.793475633015102	23.07191333317813
24-25	23.064644471227172	27.25089824612929	26.857812965427	22.826644317216537
26-27	22.73029812996468	27.300802467035457	27.010958035683906	22.95794136731596
28-29	22.55446997323884	27.63762775424146	26.928961448529094	22.878940823990607
30-31	22.81694403504279	27.219439484864278	26.78459822028177	23.179018259811162
32-33	22.646781155206618	27.271004265487576	26.795296890292114	23.28691768901369
34-35	23.146498822832747	27.363437853687422	26.6572270428508	22.83283628062903
36-37	23.218503743119506	26.82232703182353	26.938700709822726	23.02046851523424
38-39	22.732118798311134	27.418131110804683	26.712517661975312	23.13723242890887
40-41	23.034857143198252	27.192039918601203	26.900165889748685	22.872937048451863
42-43	22.733073903017477	27.214634114310517	26.63455724032046	23.417734742351552
44-45	22.826386724975915	27.15370527564968	26.727578578395732	23.292329420978675
46-47	23.267335598125367	27.377330231630797	26.402168326459563	22.95316584378427
48-49	22.901399261165274	27.201438868246314	26.7554935797429	23.14166829084551
50-51	22.66750397637545	27.35668707111631	26.983300267758036	22.99250868475021
52-53	23.128875262728414	27.308383610642018	26.632079937488395	22.930661189141176
54-55	22.83747672262986	27.220323087957492	26.697214655035268	23.24498553437738
56-57	22.868519689185053	27.329186985026944	26.66706064735803	23.135232678429976
58-59	23.200448421659626	26.82250393831456	26.677865269348484	23.29918237067733
60-61	22.732596350664306	27.257375049919148	27.046237215774273	22.963791383642278
62-63	22.61117866487107	27.28184960801906	27.143419119644175	22.963552607465694
64-65	22.58256885115162	27.51670240352243	26.7212190963749	23.179509648951054
66-67	22.760145152037744	27.33408189664693	26.46929427910189	23.436478672213436
68-69	22.76010048928384	27.11670552264247	26.955471380044276	23.16772260802941
70-71	23.035454083639713	27.003824597408443	26.70693626884765	23.253785050104195
72-73	22.922960657446325	27.031970339223342	26.820056482504572	23.22501252082576
74-75	22.640219817348164	27.676606321957443	26.602919396923006	23.080254463771386
76-77	22.950718387974277	26.982991376001443	26.76606321957439	23.300227016449888
78-79	23.041457873999413	27.222614047880068	26.737569368313697	22.998358709806823
80-81	23.12974082636853	27.21857392122416	26.619782964394297	23.03190228801302
82-83	22.992639074687933	27.24321378321475	26.88970312148362	22.874444020613698
84-85	22.898887779905543	27.3114144631592	26.822997502399705	22.966700254535557
86-87	22.834998667314952	27.274854576153924	26.817115317401402	23.073031439129725
88-89	32.28474658073963	17.367433222038272	32.4020005722054	17.9458196250167
90-91	30.942915237777306	13.5259020970532	15.391014941377602	40.14016772379189
92-93	23.266155293255448	16.43449953369673	33.00601163280232	27.293333540245502
94-95	14.872451172651168	6.410179467507097	25.957447498738865	52.75992186110287
96-97	25.669873268391697	6.787933462291347	14.066312176701393	53.475881092615566
98-99	57.63766829917578	14.008293373833212	18.67374276889473	9.68029555809628
100-101	31.094777553314767	6.748184996464398	11.855678372611617	50.30135907760922
102-103	23.180207431367986	1.8966225331446436	35.98153537865697	38.941634656830395
104-105	43.01146557357571	4.453038674033149	37.60601200023763	14.929483752153509
106-107	4.789751111967763	1.4158702260524696	5.81620802250234	87.97817063947743
108-109	18.431797005855497	9.336345329402345	18.963518597960004	53.268339066782154
110-111	40.209914183527296	2.4627792688901433	22.836430831157966	34.49087571642459
112-113	27.583457953172815	2.683760588018946	37.048529236965635	32.684252221842605
114-115	26.914253069515226	3.5872975570830086	22.14720977892236	47.3512395944794
116-117	18.318352273334305	3.827827022176005	18.97602911691281	58.87779158757688
118-119	18.887653959347436	4.002440718975744	14.293645564881565	62.816259756795255
120-121	24.456228324625055	4.022583567435008	28.353449301355198	43.16773880658474
122-123	33.60817848360985	1.4669649125187998	32.51825686567956	32.406599738191794
124-125	37.90359411462389	1.6104414270570375	17.835529995075248	42.650434463243826
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	39.0
2	85.0
3	148.5
4	191.0
5	202.0
6	193.5
7	180.0
8	188.5
9	180.5
10	180.0
11	186.0
12	174.5
13	165.5
14	178.5
15	206.0
16	248.0
17	325.5
18	455.5
19	665.5
20	1006.0
21	1564.5
22	2350.5
23	3436.5
24	5006.0
25	7108.0
26	9559.5
27	12152.0
28	14840.0
29	18083.0
30	21875.0
31	25745.5
32	29873.5
33	34338.5
34	38289.5
35	42056.5
36	45831.0
37	48751.5
38	51707.5
39	54362.0
40	56381.5
41	58021.0
42	59887.5
43	63001.0
44	65560.5
45	66394.5
46	65932.5
47	65115.0
48	64423.0
49	63895.0
50	63104.5
51	61401.0
52	58852.5
53	55031.0
54	50478.0
55	46198.0
56	42047.5
57	37323.5
58	32200.0
59	27264.5
60	22834.5
61	18715.5
62	14878.0
63	11598.5
64	8831.0
65	6561.5
66	4825.5
67	3582.0
68	2617.0
69	1870.0
70	1296.0
71	888.0
72	614.5
73	404.5
74	288.0
75	219.5
76	143.0
77	91.0
78	58.5
79	39.0
80	32.0
81	31.0
82	22.5
83	12.0
84	12.5
85	11.5
86	8.5
87	10.5
88	8.5
89	6.0
90	6.5
91	7.5
92	4.0
93	2.0
94	2.5
95	3.5
96	6.0
97	7.5
98	6.5
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.0
2	0.001014798750484268
3	0.04226338325546245
4	0.047516459140322195
5	0.04942666855299846
6	0.0
7	0.0
8	0.0
9	0.0
10-11	7.163285297536009E-4
12-13	0.0018803623906032026
14-15	5.9694044146133406E-5
16-17	2.9847022073066704E-4
18-19	0.0077303787169242764
20-21	0.0023579147437722695
22-23	7.163285297536009E-4
24-25	0.0
26-27	0.0
28-29	0.0011938808829226682
30-31	0.0
32-33	0.0011640338608496015
34-35	5.9694044146133406E-5
36-37	1.1938808829226681E-4
38-39	0.0
40-41	0.0
42-43	0.0
44-45	1.7908213243840023E-4
46-47	0.0
48-49	2.0892915451146694E-4
50-51	1.4923511036533352E-4
52-53	0.0
54-55	0.001820668346457069
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	5.9694044146133406E-5
66-67	0.0
68-69	3.283172428037337E-4
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.4923511036533352E-4
80-81	0.0
82-83	7.163285297536009E-4
84-85	5.9694044146133406E-5
86-87	0.0011640338608496015
88-89	19.358509893392405
90-91	26.23830817527843
92-93	40.376782837245976
94-95	44.79712680626716
96-97	48.38718631525977
98-99	93.00373863798487
100-101	51.628184901107865
102-103	53.05200724208143
104-105	74.8792538722034
106-107	81.72756354580234
108-109	12.612456117415796
110-111	39.138370197390294
112-113	52.039208242076064
114-115	86.8341203993054
116-117	66.47594419561977
118-119	71.77596944620045
120-121	80.42936732073431
122-123	75.8084812104042
124-125	49.93932100412545
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1675209.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.29790856544823
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.6836439519653	35.338537251821315
2	14.3964242020445	14.194272079635917
3	5.524831744871943	8.170879505945486
4	2.7089524758695203	5.341827658542409
5	1.5329424307394968	3.7785427893345833
6	0.9113021484893756	2.6955173995035464
7	0.6236514257274575	2.152129767355654
8	0.4528667579324661	1.7860307219908556
9	0.31867268206423033	1.4138907068437723
>10	1.6794999301599156	14.923119502330506
>50	0.11151262252123331	3.7591322675708203
>100	0.05170406352446521	4.594335834133537
>500	0.0031480201921899207	1.0997059664841426
>1k	8.475438978972864E-4	0.7520785485073924
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	4049	0.24170118474769417	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	2150	0.12834219491418683	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	2.9847022073066703E-5	0.0
22-23	0.0	0.0	0.0	2.6862319865760036E-4	0.0
24-25	0.0	0.0	0.0	5.969404414613341E-4	0.0
26-27	0.0	0.0	0.0	7.760225738997343E-4	0.0
28-29	0.0	0.0	0.0	0.001999750478895469	0.0
30-31	0.0	0.0	0.0	0.00516353481864054	0.0
32-33	0.0	0.0	0.0	0.012147737983738149	0.0
34-35	0.0	0.0	0.0	0.021340620782242692	0.0
36-37	0.0	0.0	0.0	0.03712969545889498	0.0
38-39	0.0	0.0	0.0	0.061663947602955814	0.0
40-41	0.0	0.0	0.0	0.09533138850137504	0.0
42-43	0.0	0.0	0.0	0.12858097109077135	0.0
44-45	0.0	0.0	0.0	0.16618821890283542	0.0
46-47	0.0	0.0	0.0	0.21185416267462748	0.0
48-49	0.0	0.0	0.0	0.2628328763754254	0.0
50-51	0.0	0.0	0.0	0.31858711360791403	0.0
52-53	0.0	0.0	0.0	0.37431150381832956	0.0
54-55	0.0	0.0	0.0	0.4334384545450747	0.0
56-57	0.0	0.0	0.0	0.5047131432555579	0.0
58-59	0.0	0.0	0.0	0.5830914232194311	0.0
60-61	0.0	0.0	0.0	0.6691702348781555	0.0
62-63	0.0	0.0	0.0	0.7534283781904227	0.0
64-65	0.0	0.0	0.0	0.8444021014691301	0.0
66-67	0.0	0.0	0.0	0.9510455113361974	0.0
68-69	0.0	0.0	0.0	1.0686427783040804	0.0
70-71	0.0	0.0	0.0	1.2042079525599494	0.0
72-73	0.0	0.0	0.0	1.345503755053847	0.0
74-75	0.0	0.0	0.0	1.4869786396801832	0.0
76-77	0.0	0.0	0.0	1.6388999820320926	0.0
78-79	0.0	0.0	0.0	1.6807156599564592	0.0
80-81	0.0	0.0	0.0	1.6814021414641398	0.0
82-83	0.0	0.0	0.0	1.6814021414641398	0.0
84-85	0.0	0.0	0.0	1.6814021414641398	0.0
86-87	0.0	0.0	0.0	1.6814021414641398	0.0
88-89	0.0	0.0	0.0	1.6814021414641398	0.0
90-91	0.0	0.0	0.0	1.6814021414641398	0.0
92-93	0.0	0.0	0.0	1.6814021414641398	0.0
94-95	0.0	0.0	0.0	1.6814021414641398	0.0
96-97	0.0	0.0	0.0	1.6814021414641398	0.0
98-99	0.0	0.0	0.0	1.6814021414641398	0.0
100-101	0.0	0.0	0.0	1.6814021414641398	0.0
102-103	0.0	0.0	0.0	1.6814021414641398	0.0
104-105	0.0	0.0	0.0	1.6814021414641398	0.0
106-107	0.0	0.0	0.0	1.6814021414641398	0.0
108-109	0.0	0.0	0.0	1.6814021414641398	0.0
110-111	0.0	0.0	0.0	1.6814021414641398	0.0
112-113	0.0	0.0	0.0	1.6814021414641398	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCTCC	725	0.0	1749.1663	108-109
CGTCGCT	95	3.6379788E-11	1304.7584	110-111
CCGCGCC	665	0.0	1046.9727	108-109
CCTCGCC	650	0.0	994.62384	108-109
CCGTTCG	85	9.199903E-8	877.6094	108-109
TTGTTCG	230	0.0	864.8904	108-109
CCACGCG	80	9.024813E-5	621.63995	108-109
GTCGCGC	50	1.216959E-5	619.7603	110-111
CTCCGCC	470	0.0	593.38745	110-111
CGTCGCC	300	0.0	580.19727	108-109
CCGCGCA	95	1.5105796E-4	523.48627	108-109
TTTTTCG	945	0.0	412.5442	92-93
CCGCTCG	255	1.5090336E-8	390.0486	108-109
CCGGTCG	130	3.8667573E-4	382.54764	108-109
CGCCGCC	570	0.0	380.55453	110-111
CAGCGCG	265	1.8284481E-8	375.3298	108-109
CGGGTCG	85	0.003216004	364.56488	110-111
AAGCGCG	205	3.1006966E-6	363.88678	108-109
CCGCGCG	430	1.8189894E-12	346.96182	108-109
CGTGTCT	360	2.8003342E-8	344.31125	110-111
>>END_MODULE