FastQCFastQC Report
Fri 27 May 2016
SRR939967_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939967_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1675209
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54160.32330294309545854No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42210.2519685603408291No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37610.22450930003360775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24640.14708612477607272No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20510.1224324845437196No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG69800.031.3264081
TACATGG68800.030.1336542
GGACCGA4700.029.1143636
GTATCAA133050.028.5249251
ACATGGG68000.028.1227073
AGGACCG5800.026.7058475
GAGTACT67850.026.65637212-13
CATGGGG32450.025.518854
AGAGTAC106550.025.35010110-11
AGTACTT72850.025.31683712-13
GTACTTT70150.025.2337614-15
ATCAACG151650.024.9062213
CAACGCA152900.024.7415715
TATCAAC153250.024.7239382
TCAACGC153700.024.6127934
AACGCAG156000.024.2936156
ACGCAGA167150.022.6374787
CATGGGA37750.022.5673274
CGCAGAG168900.022.2972538
TACTTTT81750.022.16267814-15
Produced by FastQC (version 0.11.5)