FastQCFastQC Report
Fri 27 May 2016
SRR939966_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939966_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533396
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40520.759660739863066No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21220.39782825518001635No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15640.29321554717320714No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC15100.2830917367209353No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT12900.2418465830264944No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT9400.17622929305806567No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC9300.174354513344682No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC8890.16666791651980892No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG7810.1464202956152652No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC6870.12879736630945865No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6710.12579771876804474No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA6620.12411041702599945No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA6610.12392293905466108No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCG52.1061054E-46215.123110-111
CGTTGCG251.3920635E-62486.0488110-111
CGTTGCC150.00189509392071.7075110-111
TGTCGTT150.00189509392071.7075110-111
CGGCGCG353.432433E-91987.5773108-109
CCGCTCG353.432433E-91987.5773108-109
CGGCTCG253.3673532E-61855.0721108-109
CCGCGCC1000.01855.0721108-109
TCGCGTT200.00336869921553.7808110-111
GACCGGT150.00340906531545.8934108-109
CCGCACG150.00340906531545.8934108-109
CCGCACC305.817861E-61545.8934108-109
CCGCTCC1100.01475.6255108-109
CCTTACG200.0060596981159.42108-109
GGTCGGC200.0060596981159.42108-109
CCTCGCG602.9616785E-81159.42108-109
CATCGCG200.0060596981159.42108-109
CGTCGCA200.0060596981159.42108-109
CCGCGCG401.3786092E-51159.42108-109
CTCGTCC300.007577971035.8538110-111
Produced by FastQC (version 0.11.5)