##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR939966_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	533396
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.555066029741507	33.0	31.0	34.0	28.0	34.0
2	31.62011713623649	34.0	31.0	34.0	28.0	34.0
3	31.811940846950485	34.0	31.0	34.0	28.0	34.0
4	34.843710863973484	37.0	35.0	37.0	32.0	37.0
5	34.991308521248754	37.0	35.0	37.0	32.0	37.0
6	34.91630608403513	37.0	36.0	37.0	32.0	37.0
7	34.845356920561834	37.0	36.0	37.0	32.0	37.0
8	34.841646731509044	37.0	36.0	37.0	32.0	37.0
9	36.31144590510615	39.0	38.0	39.0	31.0	39.0
10-11	36.34567469572325	39.0	38.0	39.0	31.5	39.0
12-13	36.26758730849125	39.0	38.0	39.0	31.0	39.0
14-15	37.763371866305704	41.0	39.0	41.0	31.0	41.0
16-17	37.867197166832895	41.0	39.0	41.0	31.5	41.0
18-19	37.990700155231764	41.0	39.0	41.0	32.0	41.0
20-21	38.125102175494376	41.0	39.0	41.0	32.0	41.0
22-23	38.219031451304474	41.0	39.0	41.0	32.0	41.0
24-25	38.34168610188303	41.0	39.0	41.0	32.0	41.0
26-27	38.37313740635475	41.0	39.0	41.0	33.0	41.0
28-29	38.367525253282736	41.0	39.0	41.0	33.0	41.0
30-31	38.340218149367445	41.0	39.0	41.0	33.0	41.0
32-33	38.23086974780463	40.5	38.0	41.0	32.0	41.0
34-35	38.12365672033536	40.0	38.0	41.0	32.0	41.0
36-37	38.293099310830975	41.0	39.0	41.0	33.0	41.0
38-39	38.272008976445264	41.0	39.0	41.0	33.0	41.0
40-41	38.24036925661235	41.0	39.0	41.0	33.0	41.0
42-43	38.137128887355736	41.0	38.0	41.0	32.0	41.0
44-45	38.03122914307569	41.0	38.0	41.0	32.0	41.0
46-47	37.93397119588448	41.0	38.0	41.0	32.0	41.0
48-49	37.83013745884858	41.0	38.0	41.0	31.0	41.0
50-51	37.720713128707374	40.0	38.0	41.0	31.0	41.0
52-53	37.63487540214025	40.0	38.0	41.0	31.0	41.0
54-55	37.477534514694526	40.0	37.5	41.0	30.5	41.0
56-57	37.28871138891181	40.0	37.0	41.0	30.0	41.0
58-59	37.137851614935244	40.0	37.0	41.0	30.5	41.0
60-61	36.890003862046214	40.0	36.0	41.0	31.0	41.0
62-63	36.69267298592415	40.0	36.0	41.0	31.0	41.0
64-65	36.41886609573375	39.0	35.0	41.0	30.0	41.0
66-67	36.08891236529708	39.0	35.0	41.0	30.0	41.0
68-69	35.70168223983681	38.5	35.0	41.0	30.0	41.0
70-71	35.27721711448905	37.5	35.0	40.0	29.5	41.0
72-73	34.86222150147358	37.0	34.5	39.5	29.0	41.0
74-75	34.4448299199844	36.5	34.0	39.0	29.0	41.0
76-77	33.975353208498	36.0	34.0	39.0	28.5	40.5
78-79	33.45915417438451	35.5	34.0	38.0	27.5	39.5
80-81	32.93837036648194	35.0	34.0	37.0	26.0	39.0
82-83	32.666518871532595	35.0	34.0	37.0	26.0	39.0
84-85	32.21792533127358	35.0	34.0	36.5	25.5	38.0
86-87	31.528971908300775	35.0	34.0	36.0	10.5	37.0
88-89	16.315656660342412	18.0	16.5	18.5	2.0	19.0
90-91	2.849252712806245	2.0	2.0	2.0	2.0	2.0
92-93	2.3175061305296625	2.0	2.0	2.0	2.0	2.0
94-95	2.106614972740703	2.0	2.0	2.0	2.0	2.0
96-97	2.0	2.0	2.0	2.0	2.0	2.0
98-99	2.0	2.0	2.0	2.0	2.0	2.0
100-101	2.0	2.0	2.0	2.0	2.0	2.0
102-103	2.0	2.0	2.0	2.0	2.0	2.0
104-105	2.0	2.0	2.0	2.0	2.0	2.0
106-107	2.0	2.0	2.0	2.0	2.0	2.0
108-109	2.0	2.0	2.0	2.0	2.0	2.0
110-111	2.0	2.0	2.0	2.0	2.0	2.0
112-113	2.0	2.0	2.0	2.0	2.0	2.0
114-115	2.0	2.0	2.0	2.0	2.0	2.0
116-117	2.0	2.0	2.0	2.0	2.0	2.0
118-119	2.0	2.0	2.0	2.0	2.0	2.0
120-121	2.0	2.0	2.0	2.0	2.0	2.0
122-123	2.0	2.0	2.0	2.0	2.0	2.0
124-125	2.0	2.0	2.0	2.0	2.0	2.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.08766039976531204
1101	2	0.22142891303827028
1101	3	0.308117996161851
1101	4	0.3133294871657313
1101	5	0.22948976151886313
1101	6	0.3029737051193777
1101	7	0.224823612793962
1101	8	0.2522107640750946
1101	9	0.5614226595529175
1101	10-11	0.38709035206878184
1101	12-13	0.44764205816938585
1101	14-15	0.40764768077674063
1101	16-17	0.556815891480376
1101	18-19	0.49931080609148637
1101	20-21	0.25251520926033066
1101	22-23	0.43147223388061207
1101	24-25	0.3908570611999167
1101	26-27	0.27929020896002044
1101	28-29	0.30365820018466394
1101	30-31	0.279022521971207
1101	32-33	0.2913361763275546
1101	34-35	0.39030798974813763
1101	36-37	0.42413727106471555
1101	38-39	0.2980425357611125
1101	40-41	0.2984639935548117
1101	42-43	0.2858487716024172
1101	44-45	0.3167698813079127
1101	46-47	0.3140153965675907
1101	48-49	0.2428641299342047
1101	50-51	0.2651717400243996
1101	52-53	0.19712482987373647
1101	54-55	0.172963138593083
1101	56-57	0.29554371420845627
1101	58-59	0.33332316310826116
1101	60-61	0.07878222864370343
1101	62-63	0.10665244358338555
1101	64-65	0.12676580625240064
1101	66-67	0.22409397930604058
1101	68-69	0.257915975677399
1101	70-71	0.22511094462474546
1101	72-73	0.16500544228198066
1101	74-75	0.17084493619487517
1101	76-77	0.10442027096827644
1101	78-79	-0.030412766155038184
1101	80-81	0.1285616813204129
1101	82-83	0.144432045842386
1101	84-85	0.1153419900597612
1101	86-87	0.29500443697821765
1101	88-89	0.9747811856801114
1101	90-91	-0.13095848842335744
1101	92-93	-0.28371052944789366
1101	94-95	-0.07497908929070585
1101	96-97	0.0
1101	98-99	0.0
1101	100-101	0.0
1101	102-103	0.0
1101	104-105	0.0
1101	106-107	0.0
1101	108-109	0.0
1101	110-111	0.0
1101	112-113	0.0
1101	114-115	0.0
1101	116-117	0.0
1101	118-119	0.0
1101	120-121	0.0
1101	122-123	0.0
1101	124-125	0.0
1102	1	-0.00697215683272745
1102	2	0.09321235303132624
1102	3	0.041623878886131394
1102	4	0.2515825824019515
1102	5	0.27360032990603145
1102	6	0.04814639917825758
1102	7	0.1160938235619895
1102	8	0.21938034908854576
1102	9	0.325348263410028
1102	10-11	0.09469409870525425
1102	12-13	0.1427800319239907
1102	14-15	0.036317972032748
1102	16-17	0.17767214152969757
1102	18-19	0.06881256972512517
1102	20-21	0.07681666041123236
1102	22-23	0.10041610535235179
1102	24-25	0.27843034866035765
1102	26-27	0.16532475566278038
1102	28-29	0.21400879634806813
1102	30-31	0.2528974657479921
1102	32-33	0.26870090523299694
1102	34-35	0.2039684567362272
1102	36-37	0.1669623990958442
1102	38-39	0.23564265254759675
1102	40-41	0.0905130438755819
1102	42-43	0.09420452846559613
1102	44-45	0.061595372324035225
1102	46-47	0.20882471107830725
1102	48-49	0.08492620036907539
1102	50-51	0.0646659283189237
1102	52-53	0.19726094924845938
1102	54-55	0.1091034244043172
1102	56-57	0.16057642231327662
1102	58-59	-0.10892079437514468
1102	60-61	-0.18095523851010142
1102	62-63	-0.006397656237496108
1102	64-65	-0.0643773597658921
1102	66-67	-0.18553261960342837
1102	68-69	-0.11474730838992286
1102	70-71	0.10532984036125015
1102	72-73	0.09027985105043967
1102	74-75	0.012849797037475241
1102	76-77	-0.031054016279114194
1102	78-79	0.09478746741792321
1102	80-81	0.16965592327358792
1102	82-83	0.2407078986598563
1102	84-85	0.1703776178265315
1102	86-87	0.3011258633514444
1102	88-89	1.3729323294774076
1102	90-91	0.1403688530271623
1102	92-93	0.026738309394657822
1102	94-95	0.1538000974650764
1102	96-97	0.0
1102	98-99	0.0
1102	100-101	0.0
1102	102-103	0.0
1102	104-105	0.0
1102	106-107	0.0
1102	108-109	0.0
1102	110-111	0.0
1102	112-113	0.0
1102	114-115	0.0
1102	116-117	0.0
1102	118-119	0.0
1102	120-121	0.0
1102	122-123	0.0
1102	124-125	0.0
1103	1	-0.146561970882793
1103	2	-0.048287905554094124
1103	3	-0.10942088340825507
1103	4	0.06372606257578184
1103	5	-0.13316611728465233
1103	6	0.07528629003205367
1103	7	-0.24063827422189377
1103	8	0.06634023361278452
1103	9	-0.11583706128835303
1103	10-11	0.0556766461190179
1103	12-13	0.02958136884922169
1103	14-15	-0.03143719774595155
1103	16-17	0.0401046970483705
1103	18-19	-0.07052251838688761
1103	20-21	-0.13410027577606343
1103	22-23	0.0435987129740667
1103	24-25	-0.08439587421711536
1103	26-27	-0.19194574012652765
1103	28-29	-0.07046990316023738
1103	30-31	-0.05645560755905166
1103	32-33	6.558882126697085E-4
1103	34-35	-0.020126687261118548
1103	36-37	-0.08823246624675818
1103	38-39	-0.044501846529989564
1103	40-41	-0.02249264500370174
1103	42-43	-0.14412246244918236
1103	44-45	-0.25879196548115857
1103	46-47	-0.2159307906159995
1103	48-49	-0.12088849808944957
1103	50-51	-0.30852107911444193
1103	52-53	-0.27239578825260224
1103	54-55	-0.3137126866253155
1103	56-57	-0.25661449091780497
1103	58-59	-0.2945619704370799
1103	60-61	-0.202099421110006
1103	62-63	-0.12847313230483337
1103	64-65	-0.12650926148798902
1103	66-67	-0.19857381573468302
1103	68-69	-0.23304627945151424
1103	70-71	-0.17077377906276325
1103	72-73	-0.18920660602051953
1103	74-75	-0.13107228770135748
1103	76-77	-0.043363840209458715
1103	78-79	-0.07313095853015739
1103	80-81	-0.20524855449964008
1103	82-83	-0.38616877539129035
1103	84-85	-0.26473094945585274
1103	86-87	-0.18786881827972834
1103	88-89	0.48028580417077293
1103	90-91	-0.6390095384943235
1103	92-93	-0.3401172146846623
1103	94-95	-0.1181545767558867
1103	96-97	0.0
1103	98-99	0.0
1103	100-101	0.0
1103	102-103	0.0
1103	104-105	0.0
1103	106-107	0.0
1103	108-109	0.0
1103	110-111	0.0
1103	112-113	0.0
1103	114-115	0.0
1103	116-117	0.0
1103	118-119	0.0
1103	120-121	0.0
1103	122-123	0.0
1103	124-125	0.0
1104	1	-0.11717891341807274
1104	2	-0.042603545585301106
1104	3	0.20372542369876712
1104	4	0.09784701096734238
1104	5	0.15896746794746974
1104	6	0.2610530223846581
1104	7	0.18688844689138762
1104	8	0.10989814383871988
1104	9	0.0771058289175457
1104	10-11	0.20032003802744214
1104	12-13	0.21538507264877182
1104	14-15	0.2645016736636663
1104	16-17	0.2329052471835027
1104	18-19	0.02929761552368859
1104	20-21	0.18380425655362131
1104	22-23	0.1331102831883868
1104	24-25	0.2059963558635971
1104	26-27	0.20124097677201291
1104	28-29	0.2802024226025779
1104	30-31	0.18573232570009424
1104	32-33	0.23826398348069233
1104	34-35	0.23156290786457134
1104	36-37	0.17320372658151229
1104	38-39	0.17417686789885778
1104	40-41	0.3372986881497013
1104	42-43	0.17184106891554052
1104	44-45	0.17609946786487285
1104	46-47	0.1798032589775005
1104	48-49	0.060546104966611836
1104	50-51	0.04305334995399335
1104	52-53	0.12619245704555482
1104	54-55	0.1575983948264792
1104	56-57	0.21723652893955858
1104	58-59	0.12174021938171364
1104	60-61	0.19113166152487793
1104	62-63	0.11936773519319388
1104	64-65	0.20048747393324362
1104	66-67	0.14225671084964375
1104	68-69	0.10480712136703829
1104	70-71	0.16515433365578502
1104	72-73	0.1667997752512207
1104	74-75	0.2561962248264251
1104	76-77	0.18659486268430214
1104	78-79	0.09872156976224034
1104	80-81	0.03915211985955125
1104	82-83	0.020173306618779918
1104	84-85	-0.05044814006953402
1104	86-87	0.07976101619910025
1104	88-89	0.8906790324805485
1104	90-91	-0.5374018351236369
1104	92-93	-0.3401172146846623
1104	94-95	-0.1181545767558867
1104	96-97	0.0
1104	98-99	0.0
1104	100-101	0.0
1104	102-103	0.0
1104	104-105	0.0
1104	106-107	0.0
1104	108-109	0.0
1104	110-111	0.0
1104	112-113	0.0
1104	114-115	0.0
1104	116-117	0.0
1104	118-119	0.0
1104	120-121	0.0
1104	122-123	0.0
1104	124-125	0.0
1105	1	-0.09677760868346752
1105	2	-0.16971093458850817
1105	3	0.025045074691245617
1105	4	-0.1508037748387281
1105	5	0.08553211610943379
1105	6	-0.313626859362536
1105	7	-0.11002853352860598
1105	8	-0.1472819400884262
1105	9	0.05157005205231968
1105	10-11	0.07781606182177825
1105	12-13	0.08926522037492646
1105	14-15	-0.15362443270995385
1105	16-17	0.022433224395577156
1105	18-19	-0.004996596738301662
1105	20-21	-0.13665725287695096
1105	22-23	-0.18052023969389097
1105	24-25	-0.019318370923905093
1105	26-27	0.00732614395236908
1105	28-29	0.14094536652235234
1105	30-31	0.1361901509627117
1105	32-33	0.024921005414974218
1105	34-35	0.07651784420034602
1105	36-37	-0.017703567172468127
1105	38-39	0.018628600805662643
1105	40-41	0.005538464583608516
1105	42-43	0.07648132339271285
1105	44-45	0.07975757707799147
1105	46-47	0.049091675414274505
1105	48-49	0.01821231934924583
1105	50-51	-0.1452462717448597
1105	52-53	-0.07131399024837748
1105	54-55	-0.08127047575139557
1105	56-57	-0.055160965826225095
1105	58-59	-0.4009156164301899
1105	60-61	-0.3382859703638488
1105	62-63	-0.20382275426199215
1105	64-65	-0.19409050911958303
1105	66-67	-0.0703027075909688
1105	68-69	-0.15823078961987136
1105	70-71	-0.1554716154667588
1105	72-73	-0.22611134644313324
1105	74-75	-0.1338295643493126
1105	76-77	-0.08395066374735904
1105	78-79	0.062083867245320334
1105	80-81	-0.01139017169291634
1105	82-83	-0.12945513156358146
1105	84-85	-0.17203556717242918
1105	86-87	-0.304819393013112
1105	88-89	0.7911714529789009
1105	90-91	-0.8440504104289284
1105	92-93	-0.3401172146846623
1105	94-95	-0.1181545767558867
1105	96-97	0.0
1105	98-99	0.0
1105	100-101	0.0
1105	102-103	0.0
1105	104-105	0.0
1105	106-107	0.0
1105	108-109	0.0
1105	110-111	0.0
1105	112-113	0.0
1105	114-115	0.0
1105	116-117	0.0
1105	118-119	0.0
1105	120-121	0.0
1105	122-123	0.0
1105	124-125	0.0
1106	1	0.24490380277196877
1106	2	0.2298492494264579
1106	3	0.344361590539787
1106	4	0.21582581921617106
1106	5	0.4091271483794543
1106	6	0.5160514514744605
1106	7	0.3342277393258186
1106	8	0.3993823674120378
1106	9	0.46955026875858863
1106	10-11	0.3416449885696622
1106	12-13	0.4813375793726209
1106	14-15	0.4794030515763197
1106	16-17	0.3487935364795689
1106	18-19	0.3266389794880453
1106	20-21	0.3543956092971925
1106	22-23	0.4311904928272199
1106	24-25	0.3074581654830908
1106	26-27	0.15156051026346518
1106	28-29	0.2510594490594684
1106	30-31	0.30236981832027254
1106	32-33	0.2909835570217325
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2216	20-21	0.188283709184752
2216	22-23	0.014227950664960076
2216	24-25	0.011109269098689367
2216	26-27	-0.030277172439653555
2216	28-29	-0.005301874095749781
2216	30-31	0.07815906620476198
2216	32-33	-0.08577320373334629
2216	34-35	-0.026561112624953864
2216	36-37	-0.10868295523140858
2216	38-39	-0.07291549796737229
2216	40-41	-0.07513110264263645
2216	42-43	-0.19344205563085382
2216	44-45	-0.17579263083823093
2216	46-47	-0.17749849861930045
2216	48-49	-0.11143884344145505
2216	50-51	-0.04596114417157082
2216	52-53	-0.19218197628462264
2216	54-55	-0.21433685492897325
2216	56-57	-0.16843487619123465
2216	58-59	-0.18142090417597245
2216	60-61	-0.08282624450522746
2216	62-63	-0.21703955183888013
2216	64-65	-0.09033839145440226
2216	66-67	-0.09432793989242327
2216	68-69	-0.10533600549337763
2216	70-71	-0.08338757624503046
2216	72-73	0.05215563338128959
2216	74-75	0.020489189180125322
2216	76-77	0.008448201697476065
2216	78-79	0.047438608248455694
2216	80-81	0.06986207431560132
2216	82-83	0.11683061205775402
2216	84-85	0.12499007582772492
2216	86-87	0.13350392953937984
2216	88-89	-1.1261343635414551
2216	90-91	-0.8440504104289284
2216	92-93	-0.3401172146846623
2216	94-95	-0.1181545767558867
2216	96-97	0.0
2216	98-99	0.0
2216	100-101	0.0
2216	102-103	0.0
2216	104-105	0.0
2216	106-107	0.0
2216	108-109	0.0
2216	110-111	0.0
2216	112-113	0.0
2216	114-115	0.0
2216	116-117	0.0
2216	118-119	0.0
2216	120-121	0.0
2216	122-123	0.0
2216	124-125	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4504.0
3	816.0
4	584.0
5	567.0
6	616.0
7	862.0
8	924.0
9	1083.0
10	1312.0
11	1622.0
12	2000.0
13	2449.0
14	2838.0
15	3348.0
16	3883.0
17	4535.0
18	6060.0
19	7401.0
20	5693.0
21	9058.0
22	9164.0
23	9504.0
24	15174.0
25	26935.0
26	47180.0
27	136919.0
28	219027.0
29	8975.0
30	363.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.74068796916362	19.45815116723785	14.074533742285281	20.726627121313246
2	13.394889293012618	21.312360561690323	43.372016723223155	21.920733422073905
3	21.0134787876969	28.236428365101457	27.71479590496948	23.035296942232158
4	11.217637282450882	17.020885465711746	40.08362439926618	31.6778528525712
5	12.365431042730041	41.396872203807604	33.18101412329227	13.056682630170084
6	28.39728831862256	37.00046494536892	20.666071736570952	13.936174999437567
7	28.457468747422176	33.78315547923119	20.731876504510723	17.02749926883591
8	26.01631808262529	34.87840178778994	20.671321119768425	18.43395900981635
9	27.399343077188433	18.7449849642667	20.940914442552998	32.91475751599187
10-11	24.516605594973278	28.449378924781982	27.27734610448837	19.756669375756367
12-13	25.774416439891972	26.015424116328624	27.667325039769548	20.54283440400986
14-15	22.308566243466394	27.273732836391723	27.18355593217797	23.234144987963916
16-17	20.91603869181253	30.847430935264615	27.43447860823462	20.802051764688237
18-19	20.565515586271033	30.65581208728162	28.907536082976787	19.871136243470566
20-21	23.537445958056328	28.104341728817246	27.79480747381801	20.563404839308415
22-23	21.74151468338483	29.009902660707322	27.634272729999697	21.614309925908152
24-25	21.682014863253567	28.982969501083623	28.424285146495286	20.910730489167523
26-27	21.301903276365028	28.841048676780478	28.815645411664132	21.041402635190366
28-29	20.34256424485694	30.25081132156831	28.299421247907247	21.107203185667494
30-31	21.18641684602059	29.20503715813392	28.32792146922737	21.280624526618123
32-33	20.89126071799179	29.306671191181692	28.570343866266835	21.23172422455968
34-35	21.638185584791756	29.366698225517673	28.360689545271327	20.63442664441924
36-37	21.202164995613014	28.99628043704865	28.873669843793355	20.927884723544985
38-39	20.820553584953768	29.788843560881595	28.65835139371124	20.732251460453398
40-41	21.36273987806433	29.60117811157189	28.34020127635003	20.695880734013752
42-43	20.528087949665913	29.653109509632618	28.09179296432669	21.727009576374776
44-45	21.207695595767497	29.149074983689417	28.144005579344427	21.49922384119866
46-47	21.5727152059633	29.520187627953714	28.05551597687272	20.851581189210265
48-49	21.03615325199289	29.267279844618255	28.75837089142026	20.938196011968593
50-51	20.339953805427864	29.641954570337987	29.15648036355728	20.86161126067687
52-53	20.706285761423032	30.346309308656235	27.895784745292428	21.051620184628305
54-55	20.361407722167694	29.370634614861828	28.56174650089569	21.70621116207478
56-57	21.14985864160961	29.304681699900257	28.102104252750305	21.443355405739826
58-59	21.80443797853752	29.177009201418834	28.174564488672583	20.84398833137106
60-61	21.104488972545727	29.437509842593496	29.04314992988324	20.41485125497754
62-63	20.501934772664214	29.48287951165738	28.912196566903386	21.10298914877502
64-65	20.31351941146915	30.288941049426693	28.23568230732889	21.161857231775265
66-67	20.704410981709646	29.577555887183255	27.882942504255748	21.835090626851343
68-69	20.60341829546424	29.531519353874103	28.291067868153867	21.57399448250779
70-71	21.3083712663762	29.319773676593	28.080919241989065	21.290935815041735
72-73	21.013749634417955	28.828768869657818	29.115891382762527	21.041590113161703
74-75	20.55836564203706	30.057499493809477	28.17015875634613	21.213976107807333
76-77	20.53127507517867	29.167635302851913	28.431221831434804	21.86986779053461
78-79	20.88163638732683	29.2757049425894	28.51360484594423	21.329053824139542
80-81	21.108238531972493	29.41173162153447	28.304580461795737	21.1754493846973
82-83	20.91412898182016	29.19142170969623	28.8415858475833	21.052863460900312
84-85	20.804617957390008	29.488316372826194	28.678786492587122	21.02827917719668
86-87	20.655336620484075	29.587543823468753	28.365079960254224	21.392039595792948
88-89	30.91077824509434	18.86076450505587	33.92590160269398	16.30255564715581
90-91	30.841582463619083	15.655617248807802	17.38853688936677	36.11426339820635
92-93	22.54123138127085	18.065688286058858	35.88390997227748	23.509170360392815
94-95	16.2898181200068	7.197858235594085	29.193098759136493	47.31922488526262
96-97	28.810321836594948	7.422424045842793	16.456013262695066	47.311240854867194
98-99	57.698382951381454	14.044763332619404	19.154262154636967	9.102591561362177
100-101	31.94317855553136	6.970492852036481	13.096603007173977	47.98972558525818
102-103	23.947905005597313	2.0911962258116104	37.51819126819127	36.442707500399806
104-105	42.29078491191432	4.263636464979395	41.32316164585137	12.122416977254908
106-107	6.0843793584379355	2.1012798424809254	7.63034293215194	84.18399786692919
108-109	19.638269790001182	11.635780279211511	22.318249616379266	46.407700314408046
110-111	41.019978207833155	2.7324972990967193	26.346945474888923	29.9005790181812
112-113	27.740133571468917	2.7923799210413045	40.1483737897642	29.31911271772558
114-115	29.053004786385394	3.824853749335224	24.784258110264137	42.33788335401524
116-117	20.514741723883404	4.452460203612663	21.583499296686767	53.44929877581717
118-119	21.495406908194	4.485366806906224	17.50465549348231	56.514570791417476
120-121	26.62662423125703	4.337026490224701	31.856230108401736	37.18011917011654
122-123	35.10546230716988	1.6823517997477444	36.09197632676822	27.12020956631416
124-125	38.99788615739854	2.070152802747066	20.78927945296654	38.14268158688785
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19.0
1	38.5
2	71.5
3	112.5
4	146.5
5	156.5
6	130.0
7	99.0
8	100.5
9	102.0
10	91.0
11	82.0
12	86.5
13	95.0
14	97.5
15	122.5
16	164.0
17	229.0
18	327.5
19	456.5
20	662.0
21	904.0
22	1277.5
23	1887.0
24	2631.0
25	3559.5
26	4645.0
27	5878.5
28	7201.5
29	8703.0
30	10386.5
31	12181.0
32	14023.5
33	15569.5
34	17062.0
35	18326.5
36	19126.0
37	19968.0
38	20518.5
39	20839.0
40	21125.0
41	21169.0
42	21551.0
43	22680.0
44	23881.0
45	24034.0
46	22849.0
47	21415.0
48	20487.0
49	19347.0
50	18044.5
51	16357.0
52	13954.0
53	11593.5
54	9607.0
55	7850.0
56	6447.0
57	5356.0
58	4258.0
59	3331.0
60	2569.5
61	1970.0
62	1511.0
63	1100.0
64	814.5
65	587.5
66	398.5
67	269.5
68	195.5
69	133.5
70	92.5
71	80.0
72	67.5
73	55.5
74	45.0
75	28.0
76	19.0
77	13.5
78	9.5
79	8.0
80	5.5
81	3.0
82	3.0
83	2.0
84	0.5
85	1.5
86	1.5
87	1.0
88	0.5
89	0.5
90	1.0
91	0.5
92	0.5
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.0
2	0.0011248678280302066
3	0.04911922849065235
4	0.05586843545883358
5	0.05718078125820217
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0012186068136993903
12-13	0.0019685186990528614
14-15	0.0
16-17	2.8121695700755164E-4
18-19	0.007874074796211446
20-21	0.002624691598737148
22-23	7.499118853534709E-4
24-25	0.0
26-27	0.0
28-29	0.001312345799368574
30-31	0.0
32-33	0.0010311288423610226
34-35	1.8747797133836772E-4
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0017810407277144935
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	2.8121695700755164E-4
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	2.8121695700755164E-4
80-81	0.0
82-83	5.624339140151033E-4
84-85	0.0
86-87	0.0011248678280302066
88-89	19.496584151362214
90-91	26.070967911270426
92-93	40.15046981979617
94-95	44.85335473081913
96-97	47.9804872927431
98-99	92.99732281456929
100-101	51.35330973610601
102-103	53.108009808847456
104-105	74.77362034960892
106-107	81.71864805885308
108-109	12.64370186503086
110-111	39.17680297565036
112-113	51.87131137091392
114-115	86.78045954600334
116-117	66.34629805997795
118-119	71.45844738243257
120-121	80.41370763935237
122-123	75.84580686769304
124-125	49.57939317130237
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	533396.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.28180658344887
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.39317860074127	65.90227740151097
2	9.576198898323065	14.609795043330326
3	2.2159678134363254	5.071140844190937
4	0.7606504030231945	2.3209514768415103
5	0.36012376217114583	1.3735445586021644
6	0.1776158348670702	0.8129314056892585
7	0.11286940923114828	0.6026917710911014
8	0.0799915196944033	0.4881518106915691
9	0.05912357043499566	0.4059047488000729
>10	0.2411920013878718	3.2243542194811474
>50	0.010561621713904581	0.5463495356289712
>100	0.009087900080362357	1.4029707767863788
>500	0.002210570289813007	1.264509852099439
>1k	0.0012280946054516707	1.9744265552561695
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	4052	0.759660739863066	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	2122	0.39782825518001635	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	1564	0.29321554717320714	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	1510	0.2830917367209353	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	1290	0.2418465830264944	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	940	0.17622929305806567	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	930	0.174354513344682	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	889	0.16666791651980892	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	781	0.1464202956152652	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	687	0.12879736630945865	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	671	0.12579771876804474	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	662	0.12411041702599945	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	661	0.12392293905466108	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	1.8747797133836775E-4	0.0
18-19	0.0	0.0	0.0	3.7495594267673545E-4	0.0
20-21	0.0	0.0	0.0	5.624339140151032E-4	0.0
22-23	0.0	0.0	0.0	6.561728996842871E-4	0.0
24-25	0.0	0.0	0.0	7.49911885353471E-4	0.0
26-27	0.0	0.0	0.0	0.0013123457993685743	0.0
28-29	0.0	0.0	0.0	0.0029996475414138836	0.0
30-31	0.0	0.0	0.0	0.008811464652903285	0.0
32-33	0.0	0.0	0.0	0.019778925976197795	0.0
34-35	0.0	0.0	0.0	0.03346481788389864	0.0
36-37	0.0	0.0	0.0	0.05727452024387135	0.0
38-39	0.0	0.0	0.0	0.10208175539374123	0.0
40-41	0.0	0.0	0.0	0.15738775693855972	0.0
42-43	0.0	0.0	0.0	0.20781933122858065	0.0
44-45	0.0	0.0	0.0	0.26340654973040667	0.0
46-47	0.0	0.0	0.0	0.33924138913677643	0.0
48-49	0.0	0.0	0.0	0.42388769319604946	0.0
50-51	0.0	0.0	0.0	0.5168767669798798	0.0
52-53	0.0	0.0	0.0	0.6040540236522209	0.0
54-55	0.0	0.0	0.0	0.7017300467195104	0.0
56-57	0.0	0.0	0.0	0.8070926666116731	0.0
58-59	0.0	0.0	0.0	0.9357025549497935	0.0
60-61	0.0	0.0	0.0	1.0799668538946674	0.0
62-63	0.0	0.0	0.0	1.2243248918252105	0.0
64-65	0.0	0.0	0.0	1.3587465972748203	0.0
66-67	0.0	0.0	0.0	1.519227740740463	0.0
68-69	0.0	0.0	0.0	1.7091429257062294	0.0
70-71	0.0	0.0	0.0	1.9294295420288117	0.0
72-73	0.0	0.0	0.0	2.14437303616825	0.0
74-75	0.0	0.0	0.0	2.3513487165258082	0.0
76-77	0.0	0.0	0.0	2.5779158448882256	0.0
78-79	0.0	0.0	0.0	2.641564616157601	0.0
80-81	0.0	0.0	0.0	2.64287696195697	0.0
82-83	0.0	0.0	0.0	2.64287696195697	0.0
84-85	0.0	0.0	0.0	2.64287696195697	0.0
86-87	0.0	0.0	0.0	2.64287696195697	0.0
88-89	0.0	0.0	0.0	2.64287696195697	0.0
90-91	0.0	0.0	0.0	2.64287696195697	0.0
92-93	0.0	0.0	0.0	2.64287696195697	0.0
94-95	0.0	0.0	0.0	2.64287696195697	0.0
96-97	0.0	0.0	0.0	2.64287696195697	0.0
98-99	0.0	0.0	0.0	2.64287696195697	0.0
100-101	0.0	0.0	0.0	2.64287696195697	0.0
102-103	0.0	0.0	0.0	2.64287696195697	0.0
104-105	0.0	0.0	0.0	2.64287696195697	0.0
106-107	0.0	0.0	0.0	2.64287696195697	0.0
108-109	0.0	0.0	0.0	2.64287696195697	0.0
110-111	0.0	0.0	0.0	2.64287696195697	0.0
112-113	0.0	0.0	0.0	2.64287696195697	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTCG	5	2.1061054E-4	6215.123	110-111
CGTTGCG	25	1.3920635E-6	2486.0488	110-111
CGTTGCC	15	0.0018950939	2071.7075	110-111
TGTCGTT	15	0.0018950939	2071.7075	110-111
CGGCGCG	35	3.432433E-9	1987.5773	108-109
CCGCTCG	35	3.432433E-9	1987.5773	108-109
CGGCTCG	25	3.3673532E-6	1855.0721	108-109
CCGCGCC	100	0.0	1855.0721	108-109
TCGCGTT	20	0.0033686992	1553.7808	110-111
GACCGGT	15	0.0034090653	1545.8934	108-109
CCGCACG	15	0.0034090653	1545.8934	108-109
CCGCACC	30	5.817861E-6	1545.8934	108-109
CCGCTCC	110	0.0	1475.6255	108-109
CCTTACG	20	0.006059698	1159.42	108-109
GGTCGGC	20	0.006059698	1159.42	108-109
CCTCGCG	60	2.9616785E-8	1159.42	108-109
CATCGCG	20	0.006059698	1159.42	108-109
CGTCGCA	20	0.006059698	1159.42	108-109
CCGCGCG	40	1.3786092E-5	1159.42	108-109
CTCGTCC	30	0.00757797	1035.8538	110-111
>>END_MODULE