FastQCFastQC Report
Fri 27 May 2016
SRR939966_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939966_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533396
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44390.8322147147710145No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34250.6421120518339095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27210.5101275600116986No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22060.41357640477243923No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16050.30090214399808024No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14500.2718430584406332No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14230.2667811532144973No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT12930.24240901694050948No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9600.17997885248483303No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT9130.17116738783192975No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC9070.17004252000389952No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG8770.16441818086374851No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC8570.16066862143698116No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7460.13985856661842233No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA7220.1353590953063015No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC7140.13385927153559457No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA6910.1295472781948121No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG6650.12467285094001454No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG6230.1167987761438031No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6210.11642382020112638No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5860.10986209120428349No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC300.001309088339.66285328-29
GTACACG801.18478696E-437.250281
CCGTGAA650.001923758536.6703072
CATGGGT9650.035.81534
TACCTGG6450.035.1068462
TACACGG1051.4670637E-534.0512
GTACGAA350.002790211233.9967328-29
TCGAGGT452.3942279E-433.0523832-33
GTACATG62850.031.673111
TACATGG62850.031.5723482
ACATGGG60550.030.6065333
GGTCCGT400.00536209429.74993104-105
GTCCGTT400.00536209429.74993104-105
GATTGCG400.00536455229.7471476-77
CGTAAAC400.00536455229.7471440-41
ATGGGTA5850.029.5399685
CATGGGG24400.029.3061874
ATGGGGT7350.029.1865715
GAGTACT39200.028.912412-13
ACCTGGG8000.028.3048933
Produced by FastQC (version 0.11.5)