##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939966_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 533396 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.828834486947784 34.0 31.0 34.0 31.0 34.0 2 32.86921536719436 34.0 31.0 34.0 31.0 34.0 3 32.925305026659366 34.0 33.0 34.0 31.0 34.0 4 36.333258967071366 37.0 37.0 37.0 35.0 37.0 5 36.25765097601032 37.0 37.0 37.0 35.0 37.0 6 36.41339080158082 37.0 37.0 37.0 35.0 37.0 7 36.41500311213432 37.0 37.0 37.0 35.0 37.0 8 36.44633255592468 37.0 37.0 37.0 35.0 37.0 9 38.26759293283039 39.0 39.0 39.0 37.0 39.0 10-11 38.273822825817966 39.0 39.0 39.0 37.0 39.0 12-13 38.260331911000456 39.0 39.0 39.0 37.0 39.0 14-15 39.709783162978354 41.0 40.0 41.0 37.0 41.0 16-17 39.754549152974526 41.0 40.0 41.0 37.5 41.0 18-19 39.86568328221434 41.0 40.0 41.0 38.0 41.0 20-21 39.90130128459906 41.0 40.0 41.0 38.0 41.0 22-23 39.838782068106994 41.0 40.0 41.0 37.5 41.0 24-25 39.78374134789162 41.0 40.0 41.0 37.0 41.0 26-27 39.658048616787525 41.0 40.0 41.0 37.0 41.0 28-29 39.567340212525025 41.0 40.0 41.0 36.0 41.0 30-31 39.37354704572213 41.0 39.0 41.0 35.5 41.0 32-33 39.10523419748179 41.0 39.0 41.0 35.0 41.0 34-35 38.933212847490424 41.0 38.5 41.0 35.0 41.0 36-37 38.883211535144625 41.0 38.5 41.0 35.0 41.0 38-39 38.902601444330294 41.0 39.0 41.0 35.0 41.0 40-41 38.79885769672063 41.0 39.0 41.0 35.0 41.0 42-43 38.5718256229893 41.0 39.0 41.0 35.0 41.0 44-45 38.23729743005197 41.0 39.0 41.0 35.0 41.0 46-47 37.72446924986314 41.0 39.0 41.0 33.5 41.0 48-49 37.20580862998598 41.0 39.0 41.0 32.5 41.0 50-51 36.916365139596095 41.0 38.0 41.0 31.0 41.0 52-53 36.79750691793714 41.0 38.0 41.0 31.0 41.0 54-55 36.679815746649766 41.0 38.0 41.0 31.0 41.0 56-57 36.53318172614718 40.5 38.0 41.0 30.0 41.0 58-59 36.375490254895055 40.0 37.0 41.0 30.0 41.0 60-61 36.17413610150807 40.0 37.0 41.0 29.0 41.0 62-63 35.98150154856805 40.0 36.0 41.0 29.0 41.0 64-65 35.76044439778326 39.5 36.0 41.0 28.0 41.0 66-67 35.48617256222394 39.0 35.5 41.0 28.0 41.0 68-69 35.163591403010145 39.0 35.0 41.0 28.0 41.0 70-71 34.80276380025347 37.5 35.0 40.5 27.5 41.0 72-73 34.445030521413734 37.0 35.0 40.0 26.5 41.0 74-75 34.0922494731869 37.0 35.0 39.0 26.0 41.0 76-77 33.70920479343677 36.0 35.0 39.0 26.0 41.0 78-79 33.33629142325777 36.0 35.0 38.0 26.0 39.5 80-81 33.026859968953644 35.5 35.0 37.0 26.0 39.0 82-83 32.76488200136484 35.0 35.0 37.0 26.0 39.0 84-85 32.4941647481421 35.0 34.0 36.5 26.0 38.0 86-87 32.284128489902436 35.0 34.0 36.0 26.0 37.0 88-89 32.075686731809014 35.0 34.0 36.0 25.0 37.0 90-91 31.90374131039603 35.0 34.0 35.5 25.0 36.5 92-93 31.74575174916947 35.0 34.0 35.0 24.5 36.0 94-95 31.634198606663716 35.0 34.0 35.0 24.0 36.0 96-97 31.554022714831007 35.0 34.0 35.0 24.0 36.0 98-99 31.490849200218975 35.0 34.0 35.0 23.5 36.0 100-101 31.425820591080548 35.0 34.0 35.0 23.0 36.0 102-103 30.60802386969531 34.5 32.0 35.0 19.5 35.0 104-105 31.23518924026427 35.0 34.0 35.0 20.0 35.0 106-107 31.27632659412519 35.0 34.0 35.0 20.0 35.0 108-109 31.28613637897547 35.0 34.0 35.0 20.0 35.0 110-111 31.26760230672896 35.0 34.0 35.0 19.5 35.0 112-113 31.194065947251197 35.0 34.0 35.0 19.0 35.0 114-115 31.134865090851825 35.0 34.0 35.0 18.0 35.0 116-117 31.071489100030746 35.0 34.0 35.0 13.0 35.0 118-119 30.990331761018084 35.0 34.0 35.0 4.5 35.0 120-121 30.89693117308716 35.0 34.0 35.0 2.0 35.0 122-123 30.738809439890815 35.0 34.0 35.0 2.0 35.0 124-125 28.79963010596255 33.0 29.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14335211516225144 1101 2 0.14222368935824647 1101 3 0.18249648851315214 1101 4 0.11421281865384003 1101 5 0.07638116666992545 1101 6 0.08242942727027724 1101 7 0.11082553902047465 1101 8 0.0920544963925849 1101 9 0.21018725690395712 1101 10-11 0.18999324445950805 1101 12-13 0.10396511768326633 1101 14-15 0.34262002743707143 1101 16-17 0.28509853576549915 1101 18-19 0.2519000610998674 1101 20-21 0.25942002733387426 1101 22-23 0.15859968091467636 1101 24-25 0.12984532104775326 1101 26-27 0.14373702280429512 1101 28-29 0.22278702268000927 1101 30-31 0.2814181925114525 1101 32-33 0.34753551456655885 1101 34-35 0.35622844547438604 1101 36-37 0.29813376189469665 1101 38-39 0.32644242853982774 1101 40-41 0.3645030823406401 1101 42-43 0.45134673855839225 1101 44-45 0.6595852357873682 1101 46-47 0.8162212596838714 1101 48-49 0.9686114687626386 1101 50-51 1.0041543333755314 1101 52-53 1.082151556354134 1101 54-55 1.0140907248265307 1101 56-57 0.9538259798394932 1101 58-59 0.9009163138142284 1101 60-61 0.8275819311612693 1101 62-63 0.8321242671978482 1101 64-65 0.8543525076823713 1101 66-67 0.8909729375668007 1101 68-69 0.7278978618992582 1101 70-71 0.7343566524801375 1101 72-73 0.6227839126767805 1101 74-75 0.6599330044798535 1101 76-77 0.5918529817546627 1101 78-79 0.5957814537906501 1101 80-81 0.6809749051356704 1101 82-83 0.5847271604204849 1101 84-85 0.5740990173484946 1101 86-87 0.6159194265230994 1101 88-89 0.6297699990581975 1101 90-91 0.5853941797409234 1101 92-93 0.6761963771101058 1101 94-95 0.7119731995850493 1101 96-97 0.7518973932712036 1101 98-99 0.7676117973454417 1101 100-101 0.7803085922292965 1101 102-103 0.8764791659655131 1101 104-105 0.8770049032434954 1101 106-107 0.769131641664881 1101 108-109 0.7463654053194055 1101 110-111 0.7260691785020015 1101 112-113 0.7487965072292546 1101 114-115 0.7114888370105028 1101 116-117 0.7074445021731748 1101 118-119 0.7150892230284427 1101 120-121 0.769425539162377 1101 122-123 0.6555280903273832 1101 124-125 0.733195096542886 1102 1 0.06650976901659789 1102 2 0.05629488176396791 1102 3 0.17893765732485178 1102 4 0.044002839722750764 1102 5 -0.06215284739180049 1102 6 5.131821133659287E-4 1102 7 -0.0557687936789506 1102 8 0.003671594564245595 1102 9 0.09387423757895164 1102 10-11 0.13442103017209917 1102 12-13 0.07131027696267012 1102 14-15 0.09025076121914566 1102 16-17 0.18848928911718588 1102 18-19 0.01902151114603612 1102 20-21 -0.013848494764808095 1102 22-23 0.15042068196417802 1102 24-25 0.10743888832228521 1102 26-27 0.08957334813354834 1102 28-29 -0.0786976648767066 1102 30-31 0.05013559290757996 1102 32-33 0.07752793922744416 1102 34-35 -0.28418362140955367 1102 36-37 -0.11661013640637208 1102 38-39 -0.1391844702268088 1102 40-41 -0.25449089684295956 1102 42-43 -0.08716518076424506 1102 44-45 -0.08065559686865953 1102 46-47 -0.25610547416131624 1102 48-49 -0.16318759536734717 1102 50-51 -0.12603891668167932 1102 52-53 0.10562721663173846 1102 54-55 0.10738773335668128 1102 56-57 0.026340124417991717 1102 58-59 -0.18041599895258287 1102 60-61 -0.2677272768212404 1102 62-63 -0.2622380233953123 1102 64-65 -0.17024761699509128 1102 66-67 -0.06248607274670803 1102 68-69 -0.10229617725578066 1102 70-71 0.040889593368817145 1102 72-73 0.0682828986860784 1102 74-75 0.029909410454905583 1102 76-77 0.008301331356570074 1102 78-79 0.17882019060284904 1102 80-81 0.09042001154551116 1102 82-83 0.07633115956826231 1102 84-85 0.17162756296229276 1102 86-87 0.06398298046187989 1102 88-89 0.07527490330117814 1102 90-91 0.07456183935568816 1102 92-93 0.0708735177352331 1102 94-95 0.05059085801616803 1102 96-97 0.009883063254708446 1102 98-99 0.08156817601770783 1102 100-101 0.12306309600512577 1102 102-103 0.10001874316689552 1102 104-105 0.12121884344566425 1102 106-107 0.16564343473187293 1102 108-109 0.08396512755696861 1102 110-111 0.09776779047892603 1102 112-113 0.12201610526282636 1102 114-115 0.14732137467019513 1102 116-117 0.16688092850694503 1102 118-119 0.08120299515277551 1102 120-121 0.10288841605522592 1102 122-123 0.24846803209196366 1102 124-125 0.12258922723327004 1103 1 0.030728493688734204 1103 2 0.05169823642738436 1103 3 0.08578901316155196 1103 4 0.034863581824318146 1103 5 -0.06884133300750506 1103 6 0.04670348554599002 1103 7 0.027020995670419268 1103 8 -0.11884772215272221 1103 9 -0.1097637119544359 1103 10-11 0.009900571047900542 1103 12-13 -8.408449893053671E-4 1103 14-15 -0.017193411346106302 1103 16-17 0.07384717906014515 1103 18-19 0.016045849051572247 1103 20-21 0.03835295485734491 1103 22-23 -0.03186467283818217 1103 24-25 0.034602707976318925 1103 26-27 0.0020286746072954998 1103 28-29 -0.014773032878501624 1103 30-31 -0.04617396857785394 1103 32-33 -0.07458011088409222 1103 34-35 0.01745627330372912 1103 36-37 0.0037362908693694408 1103 38-39 0.00511198445126837 1103 40-41 0.037091182801340494 1103 42-43 -0.024921354008753838 1103 44-45 0.01764159883033045 1103 46-47 0.15080377393402955 1103 48-49 0.16011944201562045 1103 50-51 0.2948744687804137 1103 52-53 0.1925987567973877 1103 54-55 0.26274160943637526 1103 56-57 0.2343527989631511 1103 58-59 0.13442333073224688 1103 60-61 0.2204762106084175 1103 62-63 0.16794584241814903 1103 64-65 0.20243186830413862 1103 66-67 0.20458249484443058 1103 68-69 0.11230656606538503 1103 70-71 0.17048334409247445 1103 72-73 0.15180791972026242 1103 74-75 0.11278796793419588 1103 76-77 0.07888743258310882 1103 78-79 0.16639844163912443 1103 80-81 0.10296719373631191 1103 82-83 0.17633038767147724 1103 84-85 0.18108329854133132 1103 86-87 0.19728569522286676 1103 88-89 0.08521693385284834 1103 90-91 0.10496299411327215 1103 92-93 0.1016876372711657 1103 94-95 0.08220196097947863 1103 96-97 0.20941259216609964 1103 98-99 0.11769680489745227 1103 100-101 0.2794281568691872 1103 102-103 0.2046918062848988 1103 104-105 0.17575142134934651 1103 106-107 0.1731964447433718 1103 108-109 0.0804336997794941 1103 110-111 -0.030369005068770605 1103 112-113 0.048990810205278734 1103 114-115 0.11485346627890536 1103 116-117 -0.040942631326633006 1103 118-119 -0.05825559644435785 1103 120-121 -0.0662689042279041 1103 122-123 -0.05698889220811765 1103 124-125 0.1618286001443252 1104 1 0.13303588169297598 1104 2 0.1469329866298068 1104 3 0.27020998484511694 1104 4 0.14053537150115147 1104 5 0.05922775923256296 1104 6 0.17365788866046472 1104 7 0.11746239777557577 1104 8 0.1402869981028232 1104 9 0.2111170218548395 1104 10-11 0.2101702777534129 1104 12-13 0.21517407668668653 1104 14-15 0.30285084347590185 1104 16-17 0.20062504351542287 1104 18-19 0.1826817061791104 1104 20-21 0.3268808997255235 1104 22-23 0.3969209260438049 1104 24-25 0.29985435412999095 1104 26-27 0.2163909726045503 1104 28-29 0.21087110215601257 1104 30-31 0.16675568585002765 1104 32-33 0.1137478184560834 1104 34-35 0.058906811053425656 1104 36-37 0.13902867955759035 1104 38-39 0.16745545658773864 1104 40-41 0.10474338500558389 1104 42-43 0.12599609244733756 1104 44-45 0.09780216582444723 1104 46-47 0.29888643469512033 1104 48-49 0.1407001895043294 1104 50-51 0.11998566040711722 1104 52-53 0.27574014670197045 1104 54-55 0.32368072116038604 1104 56-57 0.22602128339263317 1104 58-59 0.2357186253761938 1104 60-61 0.2171090360773107 1104 62-63 0.15949601821737502 1104 64-65 0.0515467718641176 1104 66-67 0.03646130983561591 1104 68-69 0.05515055765069121 1104 70-71 0.11938233440226043 1104 72-73 0.024816545158834913 1104 74-75 0.19635730233724047 1104 76-77 0.04959403087725178 1104 78-79 0.06973197215350524 1104 80-81 0.03965913654887032 1104 82-83 0.04533592969137601 1104 84-85 -0.06489328374416914 1104 86-87 0.009765591998586842 1104 88-89 0.08623734578871733 1104 90-91 0.08288457027999385 1104 92-93 0.0761625502958374 1104 94-95 0.10272203832374416 1104 96-97 -0.006370333680269624 1104 98-99 0.02327542652796666 1104 100-101 0.07811400678729541 1104 102-103 0.15626660506779544 1104 104-105 0.06788167915124887 1104 106-107 0.042163166743971914 1104 108-109 0.025021819374732246 1104 110-111 0.05652313812903742 1104 112-113 0.1887142268106352 1104 114-115 0.11891108258183536 1104 116-117 0.1193757298030711 1104 118-119 0.14045668805831824 1104 120-121 0.09489240589228842 1104 122-123 0.3089997797405353 1104 124-125 0.3895911990891605 1105 1 0.03709116218396247 1105 2 0.05211688052576591 1105 3 0.09032913622847616 1105 4 0.024480486384291567 1105 5 -0.0520089530518959 1105 6 -0.05666371224530309 1105 7 0.007931185684313391 1105 8 0.11145164446762834 1105 9 0.13185709032644866 1105 10-11 0.07836602388727698 1105 12-13 0.133491580078811 1105 14-15 0.23195666895416167 1105 16-17 0.12225380518570006 1105 18-19 0.03481987146346199 1105 20-21 0.10592337548639819 1105 22-23 0.14575771740308596 1105 24-25 0.14643481725686058 1105 26-27 0.1820221784057665 1105 28-29 0.09661516929772773 1105 30-31 0.005487074062486386 1105 32-33 -0.11817774337954035 1105 34-35 -0.08937376080132253 1105 36-37 -0.15325253927300508 1105 38-39 -0.1743185152148996 1105 40-41 -0.0913621798851949 1105 42-43 0.08446024369591498 1105 44-45 0.10379837382717483 1105 46-47 0.07127853799559603 1105 48-49 0.0631870927365128 1105 50-51 -0.09088892512398417 1105 52-53 -0.17968411668908146 1105 54-55 -0.19436451323356607 1105 56-57 -0.1364846696836466 1105 58-59 -0.028381404276863975 1105 60-61 -0.03564465482998713 1105 62-63 -0.16330269142525822 1105 64-65 -0.2371922542575291 1105 66-67 -0.1587780262952947 1105 68-69 -0.09281641063851254 1105 70-71 -0.07670885324769472 1105 72-73 -0.025872214534565785 1105 74-75 -0.13868991791309782 1105 76-77 -0.3557147652984085 1105 78-79 -0.24777681144205843 1105 80-81 -0.24353117319080297 1105 82-83 -0.1604913457316286 1105 84-85 -0.2096361557777371 1105 86-87 -0.21187485117986427 1105 88-89 -0.19853895332978055 1105 90-91 -0.1697424211925025 1105 92-93 -0.37140365240528794 1105 94-95 -0.2954628350217483 1105 96-97 -0.15379815543842312 1105 98-99 -0.3533229182921822 1105 100-101 -0.23458469117383274 1105 102-103 -0.24011033024360273 1105 104-105 -0.15868566474336632 1105 106-107 -0.1095859144604816 1105 108-109 -0.2869436393041802 1105 110-111 -0.35053249280719 1105 112-113 -0.2969256053350797 1105 114-115 -0.31503627874518614 1105 116-117 -0.3865288232893853 1105 118-119 -0.21566397293532802 1105 120-121 -0.46106201825428883 1105 122-123 -0.31839874838943416 1105 124-125 -0.5222412701120973 1106 1 0.007754930446431274 1106 2 0.08128965574778135 1106 3 0.02800670561324381 1106 4 -0.008916881460272919 1106 5 -0.0785445174824062 1106 6 -0.012923085718163918 1106 7 -0.008994146705354922 1106 8 -0.020425746294549185 1106 9 -0.028155042528304364 1106 10-11 0.06763434534052237 1106 12-13 0.048377076262021035 1106 14-15 -0.036754046715749666 1106 16-17 0.03955323393045518 1106 18-19 -0.01272743824450373 1106 20-21 0.09307940062836906 1106 22-23 0.052647325275955836 1106 24-25 0.06195813087430935 1106 26-27 -0.004239048697691317 1106 28-29 0.04760433398438124 1106 30-31 0.08097449465127227 1106 32-33 0.11688704943511397 1106 34-35 0.1362223499446742 1106 36-37 0.19158425697929005 1106 38-39 0.2367282805318638 1106 40-41 0.0755572545889649 1106 42-43 0.16051554929219947 1106 44-45 0.25622241710102145 1106 46-47 0.3262150932759198 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-0.01656780391250834 2216 72-73 0.12722535207563368 2216 74-75 0.06207693356797961 2216 76-77 0.06609911118396639 2216 78-79 0.005878406998313324 2216 80-81 0.07354362531014402 2216 82-83 0.11163761366053393 2216 84-85 0.12492927365908457 2216 86-87 0.0363392511867886 2216 88-89 0.18764231880944493 2216 90-91 0.16019687240572722 2216 92-93 0.1222067742079922 2216 94-95 0.136382357236581 2216 96-97 0.22393427435776658 2216 98-99 0.15503865057685573 2216 100-101 0.15610079062707882 2216 102-103 0.16627667627623666 2216 104-105 0.0323405686361653 2216 106-107 -0.010044741915688604 2216 108-109 0.0785313594123842 2216 110-111 -0.007993733117267254 2216 112-113 -0.08201852591621517 2216 114-115 -0.07388747747201663 2216 116-117 -0.16187920061881655 2216 118-119 -0.0033834400537635645 2216 120-121 -0.011128756793226557 2216 122-123 -0.23298111914768782 2216 124-125 -0.3956363365939488 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 43.0 3 3.0 4 6.0 5 22.0 6 65.0 7 163.0 8 310.0 9 596.0 10 1308.0 11 2257.0 12 2725.0 13 5037.0 14 9588.0 15 10779.0 16 3448.0 17 1847.0 18 1527.0 19 1243.0 20 1135.0 21 1115.0 22 1005.0 23 1036.0 24 1267.0 25 1518.0 26 1861.0 27 2310.0 28 2931.0 29 3720.0 30 4706.0 31 6118.0 32 7633.0 33 10463.0 34 15365.0 35 25927.0 36 54195.0 37 179754.0 38 168110.0 39 2260.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.58593798342049 18.97774085942671 14.764423626827975 19.671897530324824 2 14.035913280189577 21.41279649641167 42.71723072538977 21.834059498008983 3 20.794869102880412 28.493464517919143 28.82567548313073 21.885990896069714 4 11.034578437033648 17.41014180833752 40.133971758318395 31.421307996310432 5 11.626461686356091 40.31175131993 33.238766513206805 14.823020480507093 6 27.819481210957715 37.685509452639316 19.5580769259612 14.936932410441772 7 26.593562756376127 35.1489325004312 20.854299619794674 17.403205123398 8 26.202483708164294 33.518436583701416 20.038957922444112 20.24012178569018 9 27.772049284209103 17.1114893999955 21.37848802765675 33.73797328813865 10-11 24.47290568358218 27.839635092876584 26.850876272038036 20.8365829515032 12-13 27.025793219296734 25.42154421855432 28.08523123532985 19.4674313268191 14-15 22.87953040517739 25.87177256672341 29.399451814411808 21.84924521368739 16-17 19.55584552175637 29.730003346484924 29.48450071288565 21.22965041887305 18-19 18.980832252210362 28.015864385934652 33.7189442740478 19.284359087807182 20-21 21.694642920754674 25.475046356274923 33.48235461753495 19.34795610543545 22-23 20.09304575327596 26.25247589256337 33.43844647525701 20.216031878903664 24-25 20.19784550315338 26.30503415848638 34.11452279357176 19.38259754478849 26-27 19.863103585328723 26.081560416651044 34.61818236357228 19.437153634447952 28-29 18.82850640049794 27.55541848832762 34.08180788757321 19.534267223601226 30-31 19.60878971720823 26.386774553989905 34.07955815191715 19.924877576884718 32-33 19.469118628561144 26.540881446430042 34.233290088414606 19.7567098365942 34-35 20.265806267763537 26.58700102737928 33.94485522951054 19.20233747534665 36-37 19.882601294347914 26.372994923096538 34.24922571597837 19.495178066577175 38-39 19.707028174189535 27.02288731074099 33.89470487217751 19.37537964289196 40-41 20.223529985226733 27.06085159993701 33.35139371123893 19.364224703597326 42-43 19.718089374498497 27.106783702914903 32.697095591268024 20.47803133131857 44-45 20.678257804707947 26.72161021080023 32.28567518316598 20.314456801325846 46-47 21.16963756758581 27.42099678287801 31.52151497199079 19.887850677545387 48-49 21.36705187140511 27.53460843350906 31.015980622276885 20.082359072808945 50-51 20.614140338510225 28.395601016880516 30.8577492144673 20.13250943014196 52-53 20.95244433779031 28.996467915019984 29.62648763770257 20.424600109487134 54-55 20.416632295705256 27.99074233777531 30.397115838888933 21.195509527630506 56-57 21.25681482425815 27.894847355435736 30.020191377513143 20.82814644279297 58-59 21.667485320474846 27.823699465312824 30.229885488455103 20.278929725757227 60-61 20.945601391836462 28.097323564481176 31.147027724242406 19.810047319439967 62-63 20.179941356890566 28.072388994293167 31.10934465200339 20.638324996812873 64-65 20.1165738025782 28.803459343527138 30.40583356455617 20.674133289338503 66-67 20.51374588485853 28.070982909508135 29.946512534777163 21.468758670856175 68-69 20.366106982429564 27.995616765030107 30.30056468364967 21.337711568890654 70-71 21.005969298607415 27.704369736555957 30.103619074758715 21.186041890077917 72-73 20.812773249143227 27.24439253387727 31.0998770144508 20.8429572025287 74-75 20.225217286968782 28.494120690818832 30.09827595257557 21.18238606963682 76-77 20.280617027499268 27.678872732453936 30.25903831299822 21.78147192704857 78-79 20.553116258839587 27.74598984619307 30.33627923718963 21.36461465777771 80-81 20.844925721227757 27.822762075456136 30.12602269233365 21.206289510982458 82-83 20.568489452489334 27.653750684294597 30.679363924738844 21.09839593847723 84-85 20.416351078748246 28.058796841371137 30.28462905608591 21.240223023794705 86-87 20.235341097421053 28.142412016588054 30.073247643401903 21.548999242588994 88-89 20.272367996760384 28.05926553629948 29.641017180481295 22.027349286458843 90-91 20.57618145038068 27.655697227737253 29.72209879806746 22.046022523814614 92-93 20.85139371123893 27.97724392383895 30.0619989651216 21.109363399800525 94-95 20.361949382205687 28.014330990767533 30.011089457141544 21.612630169885232 96-97 19.751460453396728 27.959527255547474 30.642618242356523 21.64639404869928 98-99 20.4123471672927 28.107906945351907 29.378470168386656 22.101275718968736 100-101 19.864059595553393 27.727080562171974 29.779685055460725 22.629174786813913 102-103 20.71217524006644 27.231371181528104 29.700184104058163 22.356269474347293 104-105 21.223867277359794 27.124880245527134 29.40851133238217 22.242741144730896 106-107 20.271806943385897 27.522304499898286 30.2865906762175 21.919297880498316 108-109 19.98417681472156 27.559058070527538 29.830266341328983 22.626498773421925 110-111 19.61403758755681 28.498335108217965 29.42593106448081 22.461696239744416 112-113 20.095191399962314 27.537538917007986 29.096469180917094 23.270800502112603 114-115 19.486317036770387 27.682595750744333 29.305127455171327 23.525959757313956 116-117 20.26858799134579 27.48058614651413 29.10718771912063 23.143638143019448 118-119 19.921334169485867 27.033973852422825 30.017126128735622 23.027565849355685 120-121 19.404151926072995 28.2901286298419 29.378448455260774 22.927270988824333 122-123 19.607583972642804 27.59754625150922 29.169572618806573 23.625297157041402 124-125 19.5881226951538 27.52926111809999 29.32026839799245 23.56234778875376 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 94.0 1 60.5 2 72.5 3 109.5 4 64.5 5 39.0 6 119.5 7 307.5 8 550.0 9 640.0 10 532.5 11 445.0 12 497.5 13 637.5 14 762.5 15 908.0 16 1066.5 17 1215.0 18 1400.0 19 1600.5 20 1793.5 21 2036.5 22 2377.0 23 2792.5 24 3262.5 25 3912.5 26 4707.5 27 5538.5 28 6685.5 29 8034.0 30 9310.0 31 10400.0 32 11846.0 33 13592.0 34 15185.0 35 16612.5 36 17557.0 37 18139.5 38 18557.5 39 18833.0 40 19016.0 41 19063.0 42 18926.5 43 18984.0 44 18996.0 45 18732.0 46 18440.0 47 21197.5 48 27835.0 49 26547.5 50 20593.0 51 17539.5 52 14439.0 53 12719.5 54 11424.0 55 9891.5 56 8081.0 57 6734.0 58 5550.0 59 4338.0 60 3339.5 61 2437.0 62 1709.5 63 1172.5 64 867.5 65 664.0 66 456.0 67 383.5 68 285.5 69 155.5 70 115.5 71 102.0 72 103.0 73 97.5 74 72.5 75 61.5 76 37.5 77 14.5 78 9.5 79 4.5 80 2.5 81 1.0 82 1.5 83 1.0 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.5 97 0.5 98 1.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016310583506437992 2 0.0 3 0.0 4 0.0 5 0.14848255329998725 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 9.373898566918386E-5 18-19 0.0 20-21 0.00506190522613593 22-23 4.6869492834591937E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 9.373898566918387E-4 92-93 0.0 94-95 0.0012186068136993903 96-97 0.0 98-99 0.0019685186990528614 100-101 9.373898566918386E-5 102-103 3.7495594267673545E-4 104-105 0.0024372136273987806 106-107 0.007405379867865525 108-109 2.8121695700755164E-4 110-111 0.005249383197474296 112-113 0.009280159581249203 114-115 0.005999295082827767 116-117 0.002624691598737148 118-119 9.373898566918386E-5 120-121 9.373898566918387E-4 122-123 0.0014998237707069418 124-125 0.0016873017420453097 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 533396.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.35820560791869 #Duplication Level Percentage of deduplicated Percentage of total 1 85.34833402862031 60.90303967907612 2 10.087440498473416 14.39643306295424 3 2.451951427678143 5.2490056235066005 4 0.8661340686494761 2.472230918188499 5 0.3722718139817016 1.328232432206956 6 0.205375556700044 0.8793138721105505 7 0.13734122882182007 0.6860296551298152 8 0.08710888481633987 0.49727469704007093 9 0.0638775001214789 0.41023654086495087 >10 0.33577833788370776 4.21778443500354 >50 0.021536722921928538 1.077274602780526 >100 0.016809144887657848 2.3622070524529732 >500 0.003939643333043486 2.0420983849193144 >1k 0.0021011431109565254 3.4788390437658143 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4439 0.8322147147710145 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3425 0.6421120518339095 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2721 0.5101275600116986 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2206 0.41357640477243923 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1605 0.30090214399808024 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1450 0.2718430584406332 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1423 0.2667811532144973 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1293 0.24240901694050948 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 960 0.17997885248483303 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 913 0.17116738783192975 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 907 0.17004252000389952 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 877 0.16441818086374851 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 857 0.16066862143698116 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 746 0.13985856661842233 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 722 0.1353590953063015 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 714 0.13385927153559457 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 691 0.1295472781948121 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 665 0.12467285094001454 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 623 0.1167987761438031 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 621 0.11642382020112638 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 586 0.10986209120428349 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.8747797133836775E-4 0.0 18-19 0.0 0.0 0.0 3.7495594267673545E-4 0.0 20-21 0.0 0.0 0.0 5.624339140151032E-4 0.0 22-23 0.0 0.0 0.0 6.561728996842871E-4 0.0 24-25 0.0 0.0 0.0 7.49911885353471E-4 0.0 26-27 0.0 0.0 0.0 0.0013123457993685743 0.0 28-29 0.0 0.0 0.0 0.0029996475414138836 0.0 30-31 0.0 0.0 0.0 0.008811464652903285 0.0 32-33 0.0 0.0 0.0 0.019966403947536165 0.0 34-35 0.0 0.0 0.0 0.03365229585523701 0.0 36-37 0.0 0.0 0.0 0.0575557372008789 0.0 38-39 0.0 0.0 0.0 0.1022692333650796 0.0 40-41 0.0 0.0 0.0 0.1575752349098981 0.0 42-43 0.0 0.0 0.0 0.20819428717125738 0.0 44-45 0.0 0.0 0.0 0.2638752446587526 0.0 46-47 0.0 0.0 0.0 0.339522606093784 0.0 48-49 0.0 0.0 0.0 0.42388769319604946 0.0 50-51 0.0 0.0 0.0 0.516970505965549 0.0 52-53 0.0 0.0 0.0 0.6039602846665517 0.0 54-55 0.0 0.0 0.0 0.7017300467195104 0.0 56-57 0.0 0.0 0.0 0.8076551005256882 0.0 58-59 0.0 0.0 0.0 0.9360775108924702 0.0 60-61 0.0 0.0 0.0 1.0806230267943517 0.0 62-63 0.0 0.0 0.0 1.2254497596532408 0.0 64-65 0.0 0.0 0.0 1.3608088549595423 0.0 66-67 0.0 0.0 0.0 1.5219461713248692 0.0 68-69 0.0 0.0 0.0 1.7126112681759893 0.0 70-71 0.0 0.0 0.0 1.9339290133409324 0.0 72-73 0.0 0.0 0.0 2.149997375308401 0.0 74-75 0.0 0.0 0.0 2.359316530307689 0.0 76-77 0.0 0.0 0.0 2.586727309541129 0.0 78-79 0.0 0.0 0.0 2.8582891510247546 0.0 80-81 0.0 0.0 0.0 3.1650968511199933 0.0 82-83 0.0 0.0 0.0 3.475747849627669 0.0 84-85 0.0 0.0 0.0 3.7629641017180484 0.0 86-87 0.0 0.0 0.0 4.064897374558489 0.0 88-89 0.0 0.0 0.0 4.415668658932575 0.0 90-91 0.0 0.0 0.0 4.829526280662022 0.0 92-93 0.0 0.0 0.0 5.263725262281682 0.0 94-95 0.0 0.0 0.0 5.658647608905953 0.0 96-97 0.0 0.0 0.0 6.0531012606018795 0.0 98-99 0.0 0.0 0.0 6.5109224666101735 0.0 100-101 0.0 0.0 0.0 7.026486887790684 0.0 102-103 0.0 0.0 0.0 7.575141170912418 0.0 104-105 0.0 0.0 0.0 8.088830812379545 0.0 106-107 0.0 0.0 0.0 8.576367276844971 0.0 108-109 0.0 0.0 0.0 9.101680552535075 0.0 110-111 0.0 0.0 0.0 9.709765352571072 0.0 112-113 0.0 0.0 0.0 10.375405889807947 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTTAC 30 0.0013090883 39.662853 28-29 GTACACG 80 1.18478696E-4 37.25028 1 CCGTGAA 65 0.0019237585 36.670307 2 CATGGGT 965 0.0 35.8153 4 TACCTGG 645 0.0 35.106846 2 TACACGG 105 1.4670637E-5 34.051 2 GTACGAA 35 0.0027902112 33.99673 28-29 TCGAGGT 45 2.3942279E-4 33.05238 32-33 GTACATG 6285 0.0 31.67311 1 TACATGG 6285 0.0 31.572348 2 ACATGGG 6055 0.0 30.606533 3 GGTCCGT 40 0.005362094 29.74993 104-105 GTCCGTT 40 0.005362094 29.74993 104-105 GATTGCG 40 0.005364552 29.74714 76-77 CGTAAAC 40 0.005364552 29.74714 40-41 ATGGGTA 585 0.0 29.539968 5 CATGGGG 2440 0.0 29.306187 4 ATGGGGT 735 0.0 29.186571 5 GAGTACT 3920 0.0 28.9124 12-13 ACCTGGG 800 0.0 28.304893 3 >>END_MODULE