FastQCFastQC Report
Fri 27 May 2016
SRR939965_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939965_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951889
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22650.23794791199394047No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13060.13720087110997184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC4600.02011.4431108-109
CCGCGCT501.0693839E-81500.4279108-109
CGCGACT250.0056755181198.8713110-111
CGTCGTT250.0056755181198.8713110-111
CGTTTCG250.0056755181198.8713110-111
AATCGCG451.5744432E-51111.4281108-109
CGTCGCC1700.01029.7054108-109
AACGTCG250.0081568091000.2853108-109
CGTGTCG250.0081568091000.2853108-109
GTCGTAC300.008171842999.0595110-111
CGTCGTC602.1633914E-5999.0595110-111
CTCCGCC1900.0946.47736110-111
CCACGCG654.7421083E-5769.45026108-109
CCGCGCC3300.0757.79193108-109
TTGTTCG1701.8189894E-12735.5039108-109
CGGCGCC2450.0612.41956108-109
CCTCGCG1355.667789E-7555.714108-109
CCGTGCG951.47916E-4526.46594108-109
CGGCGCT1151.5211914E-4521.24835110-111
GGCGTTG1151.5211914E-4521.24835110-111
Produced by FastQC (version 0.11.5)