FastQCFastQC Report
Fri 27 May 2016
SRR939965_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939965_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951889
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26010.2732461452963528No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20680.21725222163508562No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14830.15579547615320694No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12630.13268353768138932No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10540.11072719613316258No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGCG851.7110533E-434.997259
GTACCTG10450.033.6330641
TACCTGG10700.031.1737672
GTACATG39400.026.9125271
TACATGG39000.026.8802382
GTCCTAC14900.025.9870991
GAGTACT29500.023.99980712-13
AGAGTAC48050.023.83527410-11
GTAGGAC15900.023.6008933
ACATGGG41900.023.4560533
AGTACTT30000.023.40149512-13
GTGTAGC8800.022.338911
TAGGACC7900.021.8653244
GTACTTT31900.021.82346214-15
TCCTACA19950.021.4968382
AGACCCG2556.3171683E-721.0226445
GTATCAA76400.020.5845451
TGTAGGA18350.020.4498212
AGTACAT30300.020.4444642
AGTGGAC13450.020.3478438
Produced by FastQC (version 0.11.5)