FastQCFastQC Report
Fri 27 May 2016
SRR939964_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939964_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences702934
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39870.567194075119428No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22180.3155346021105822No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14700.20912347389655359No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT14040.19973425670119813No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT12440.17697251804579092No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT8850.12590086693772104No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA8340.11864556274130998No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC8330.1185033018747137No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC8050.11451999761001744No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC7450.10598434561423975No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG7320.10413495434848791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGCT252.701814E-61998.2607108-109
CCGCTCG750.01998.2605108-109
CGTACGT150.00206451141986.7194110-111
TGTCGCG150.00206451141986.7194110-111
CCGCTCC2550.01763.1711108-109
CGTGTCG150.00294083051665.217108-109
CCGTTCG150.00294083051665.217108-109
CCGCGCC1950.01537.1237108-109
CGTCGCG200.00366983591490.0397110-111
CGCCGCT200.0052274511248.9128108-109
CGTCGTG501.2690542E-51192.0317110-111
CTCCGCC1400.01064.314110-111
CCGGACG250.0081668105999.1304108-109
CGGTGCG250.0081668105999.1304108-109
CCGTTAC250.0081668105999.1304108-109
GTCGACT300.008255303993.3597110-111
CGCTATT300.008255303993.3597110-111
GACGGTA300.008255303993.3597110-111
CGCGTCT300.008255303993.3597110-111
TTGTTCG1051.70985E-10951.5527108-109
Produced by FastQC (version 0.11.5)