##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR939964_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	702934
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7370578176614	34.0	31.0	34.0	30.0	34.0
2	31.793797141694668	34.0	31.0	34.0	30.0	34.0
3	31.867404336680256	34.0	31.0	34.0	30.0	34.0
4	35.076808064484005	37.0	35.0	37.0	32.0	37.0
5	35.07772706968222	37.0	35.0	37.0	32.0	37.0
6	35.15983435144693	37.0	36.0	37.0	33.0	37.0
7	35.0553921136266	37.0	36.0	37.0	32.0	37.0
8	35.08146710786504	37.0	36.0	37.0	33.0	37.0
9	36.704209783564316	39.0	38.0	39.0	33.0	39.0
10-11	36.686778559580276	39.0	38.0	39.0	33.0	39.0
12-13	36.65273055507345	39.0	38.0	39.0	33.0	39.0
14-15	38.15604523326515	41.0	39.0	41.0	33.0	41.0
16-17	38.18412041528792	41.0	39.0	41.0	33.0	41.0
18-19	38.21415524074806	41.0	39.0	41.0	33.0	41.0
20-21	38.272142903885715	41.0	39.0	41.0	33.0	41.0
22-23	38.276473324664906	41.0	39.0	41.0	33.0	41.0
24-25	38.311691424799484	41.0	39.0	41.0	33.0	41.0
26-27	38.275249881212176	41.0	39.0	41.0	33.0	41.0
28-29	38.23253178819064	41.0	39.0	41.0	33.0	41.0
30-31	38.16915883994799	41.0	39.0	41.0	33.0	41.0
32-33	38.058078710092275	40.0	38.0	41.0	33.0	41.0
34-35	37.96972902150131	40.0	38.0	41.0	33.0	41.0
36-37	38.14565947869928	41.0	39.0	41.0	33.0	41.0
38-39	38.10765164297075	41.0	39.0	41.0	33.0	41.0
40-41	38.049843939829344	41.0	39.0	41.0	33.0	41.0
42-43	37.976524823098615	41.0	38.0	41.0	33.0	41.0
44-45	37.887223409310124	41.0	38.0	41.0	33.0	41.0
46-47	37.77515100990989	40.0	38.0	41.0	32.0	41.0
48-49	37.673002017259094	40.0	38.0	41.0	32.0	41.0
50-51	37.55520859710869	40.0	38.0	41.0	32.0	41.0
52-53	37.45884108607636	40.0	38.0	41.0	31.5	41.0
54-55	37.30521001971736	40.0	37.5	41.0	31.0	41.0
56-57	37.097229611883904	40.0	37.0	41.0	30.5	41.0
58-59	36.925796731983375	40.0	37.0	41.0	30.5	41.0
60-61	36.690343616897174	40.0	36.0	41.0	31.0	41.0
62-63	36.47067790148151	39.5	36.0	41.0	30.0	41.0
64-65	36.1985157923788	39.0	35.0	41.0	30.0	41.0
66-67	35.86886820099754	39.0	35.0	41.0	30.0	41.0
68-69	35.46804323023214	38.5	35.0	40.5	29.0	41.0
70-71	35.04851380072667	37.0	35.0	40.0	28.5	41.0
72-73	34.67110141208136	37.0	34.5	39.5	28.5	41.0
74-75	34.265754679671204	36.5	34.0	39.0	28.0	41.0
76-77	33.809211106590375	36.0	34.0	39.0	27.5	40.5
78-79	33.3308020383137	35.5	34.0	37.5	26.0	39.5
80-81	32.849480178793456	35.0	34.0	37.0	26.0	39.0
82-83	32.61818961666387	35.0	34.0	37.0	26.0	39.0
84-85	32.180198283195864	35.0	34.0	36.5	25.0	38.0
86-87	31.528303510713666	35.0	34.0	36.0	10.0	37.0
88-89	16.15817772365542	18.0	16.5	18.5	2.0	19.0
90-91	2.7614996571513117	2.0	2.0	2.0	2.0	2.0
92-93	2.2934123260505253	2.0	2.0	2.0	2.0	2.0
94-95	2.0994595509678007	2.0	2.0	2.0	2.0	2.0
96-97	2.0000220504343225	2.0	2.0	2.0	2.0	2.0
98-99	2.0	2.0	2.0	2.0	2.0	2.0
100-101	2.0	2.0	2.0	2.0	2.0	2.0
102-103	2.0	2.0	2.0	2.0	2.0	2.0
104-105	2.0	2.0	2.0	2.0	2.0	2.0
106-107	2.0	2.0	2.0	2.0	2.0	2.0
108-109	2.0	2.0	2.0	2.0	2.0	2.0
110-111	2.0	2.0	2.0	2.0	2.0	2.0
112-113	2.0	2.0	2.0	2.0	2.0	2.0
114-115	2.0	2.0	2.0	2.0	2.0	2.0
116-117	2.0	2.0	2.0	2.0	2.0	2.0
118-119	2.0	2.0	2.0	2.0	2.0	2.0
120-121	2.0	2.0	2.0	2.0	2.0	2.0
122-123	2.0	2.0	2.0	2.0	2.0	2.0
124-125	2.0	2.0	2.0	2.0	2.0	2.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.0137862626422951
1101	2	-0.021955181637672183
1101	3	0.03648811773259908
1101	4	-0.03812681080460578
1101	5	-0.03933608317655768
1101	6	-0.0983811541801316
1101	7	0.08850257856246913
1101	8	-0.1612949298729376
1101	9	-0.16965141679506957
1101	10-11	-0.16278691929932165
1101	12-13	-0.08900721670838152
1101	14-15	-0.07700932186548926
1101	16-17	0.07389500964096385
1101	18-19	-0.009516432811714992
1101	20-21	-0.0884570876800268
1101	22-23	-0.045033623182824556
1101	24-25	-0.2781005792694202
1101	26-27	-0.11394299459550439
1101	28-29	-0.10284951915330254
1101	30-31	-0.0746248331838757
1101	32-33	-0.06994628916766743
1101	34-35	-0.03368747485296808
1101	36-37	-0.052227053283885994
1101	38-39	0.007910084875867085
1101	40-41	-0.10330356574210242
1101	42-43	-0.21668053815842114
1101	44-45	-0.1964278993456574
1101	46-47	-0.127930446095462
1101	48-49	-0.16804325169949408
1101	50-51	-0.15324524641750514
1101	52-53	-0.20111287885644202
1101	54-55	-0.07156058343895921
1101	56-57	-0.12277202626931683
1101	58-59	-0.2718740040076426
1101	60-61	-0.12807197884516341
1101	62-63	-0.05019275697839731
1101	64-65	-0.11519214227924124
1101	66-67	-0.1450974842817203
1101	68-69	-0.22786522026442668
1101	70-71	-0.30375928913245787
1101	72-73	-0.22337284673583468
1101	74-75	-0.33362084044046725
1101	76-77	-0.272536297508033
1101	78-79	-0.1803594027820168
1101	80-81	-0.1312255720804245
1101	82-83	-0.34878431184811376
1101	84-85	-0.3215864081667661
1101	86-87	-0.4098209245953939
1101	88-89	0.3323078608678891
1101	90-91	-0.1644453956440266
1101	92-93	-0.12632032652099667
1101	94-95	-0.0022677321464774636
1101	96-97	0.0
1101	98-99	0.0
1101	100-101	0.0
1101	102-103	0.0
1101	104-105	0.0
1101	106-107	0.0
1101	108-109	0.0
1101	110-111	0.0
1101	112-113	0.0
1101	114-115	0.0
1101	116-117	0.0
1101	118-119	0.0
1101	120-121	0.0
1101	122-123	0.0
1101	124-125	0.0
1102	1	0.23936698734179274
1102	2	0.21264119088566957
1102	3	0.3228951502079198
1102	4	0.3859703114862896
1102	5	0.33362344815871836
1102	6	0.2556635730808381
1102	7	0.3670060070402812
1102	8	0.12682973294036515
1102	9	0.45226329231911677
1102	10-11	0.250294307084566
1102	12-13	0.28908016121307867
1102	14-15	0.3976618917561794
1102	16-17	0.3881926647520615
1102	18-19	0.2721504199489644
1102	20-21	0.2381102500033876
1102	22-23	0.24236214243055088
1102	24-25	0.36688161309891854
1102	26-27	0.4100339144420886
1102	28-29	0.3440416847219012
1102	30-31	0.28742106699112213
1102	32-33	0.27247938888412193
1102	34-35	0.3646222349760251
1102	36-37	0.28811471967377145
1102	38-39	0.2781243295202742
1102	40-41	0.15892150644324232
1102	42-43	0.25135181506818327
1102	44-45	0.2800418172453405
1102	46-47	0.25140967497348043
1102	48-49	0.0973076781270592
1102	50-51	0.01958113204344869
1102	52-53	-0.13763464611648857
1102	54-55	0.017733236744128078
1102	56-57	-0.09633919803339808
1102	58-59	-0.09687293440187261
1102	60-61	-0.17372014428865867
1102	62-63	-0.12978707898921016
1102	64-65	-0.18285227269809923
1102	66-67	-0.08606724626574191
1102	68-69	0.01160646753172756
1102	70-71	0.032083356727852674
1102	72-73	-0.0330805886989296
1102	74-75	-0.10024590552988144
1102	76-77	-0.11494058344444369
1102	78-79	0.027771270693946803
1102	80-81	0.003873984737552405
1102	82-83	0.1678968423243461
1102	84-85	0.2513961849124158
1102	86-87	0.3151599529404834
1102	88-89	0.9460674781890326
1102	90-91	0.1857617948198902
1102	92-93	0.054526008174823115
1102	94-95	0.14651495213616172
1102	96-97	0.0
1102	98-99	0.0
1102	100-101	0.0
1102	102-103	0.0
1102	104-105	0.0
1102	106-107	0.0
1102	108-109	0.0
1102	110-111	0.0
1102	112-113	0.0
1102	114-115	0.0
1102	116-117	0.0
1102	118-119	0.0
1102	120-121	0.0
1102	122-123	0.0
1102	124-125	0.0
1103	1	0.023161734122222555
1103	2	0.016487690570954783
1103	3	0.07715077758700062
1103	4	0.03683402550653625
1103	5	-0.007367250305676976
1103	6	-0.07466382898329016
1103	7	-0.037616786217860465
1103	8	-0.06967544734557407
1103	9	0.08012793549382735
1103	10-11	0.03845607987133093
1103	12-13	-0.08540006178398585
1103	14-15	-0.06895798415674648
1103	16-17	0.00577066876840604
1103	18-19	0.07382389708476467
1103	20-21	-0.02601839293154029
1103	22-23	0.05110069035194442
1103	24-25	0.13518845387380907
1103	26-27	0.12041047177491748
1103	28-29	0.11030538475488072
1103	30-31	0.06590164966088707
1103	32-33	0.13204568355596535
1103	34-35	0.189174392512669
1103	36-37	0.08640677390442875
1103	38-39	0.17289109176662976
1103	40-41	0.1585238682939618
1103	42-43	0.13770323527580075
1103	44-45	0.12115890069939184
1103	46-47	0.10830390122757905
1103	48-49	0.08090060856036274
1103	50-51	0.01018917872097802
1103	52-53	0.07421706882291801
1103	54-55	0.08252677462466096
1103	56-57	0.10469835779080938
1103	58-59	0.20198410569164338
1103	60-61	0.14211344714313867
1103	62-63	-0.02118091060244609
1103	64-65	0.009283883399852755
1103	66-67	0.11385282646807582
1103	68-69	0.008092165546536023
1103	70-71	0.07537695954928836
1103	72-73	-0.10858637843026742
1103	74-75	-0.017601611837143594
1103	76-77	0.0036240227639083855
1103	78-79	-0.03225410621306679
1103	80-81	0.12543686251877517
1103	82-83	-0.018778782856742282
1103	84-85	0.031342933371320214
1103	86-87	0.16388411395396218
1103	88-89	0.9881716633805091
1103	90-91	-0.4663068227381322
1103	92-93	-0.30470708970610527
1103	94-95	-0.10878272697584634
1103	96-97	0.0
1103	98-99	0.0
1103	100-101	0.0
1103	102-103	0.0
1103	104-105	0.0
1103	106-107	0.0
1103	108-109	0.0
1103	110-111	0.0
1103	112-113	0.0
1103	114-115	0.0
1103	116-117	0.0
1103	118-119	0.0
1103	120-121	0.0
1103	122-123	0.0
1103	124-125	0.0
1104	1	0.06229486465640832
1104	2	0.23985480223319655
1104	3	0.20428065300115605
1104	4	0.10800877507542594
1104	5	0.2660357064283474
1104	6	0.21901968809640238
1104	7	0.19722849870202452
1104	8	0.2758884828243495
1104	9	0.24026627809521273
1104	10-11	0.28504789169438993
1104	12-13	0.3018788128745129
1104	14-15	0.3200860180908407
1104	16-17	0.3118391425433771
1104	18-19	0.2908648379694654
1104	20-21	0.21548341869776522
1104	22-23	0.17977093506721076
1104	24-25	0.3699039215066833
1104	26-27	0.40178847258869155
1104	28-29	0.3731495458833223
1104	30-31	0.39560558874364204
1104	32-33	0.30767975381231594
1104	34-35	0.34297881405070996
1104	36-37	0.3023810817070256
1104	38-39	0.30612141088464284
1104	40-41	0.34838746326402514
1104	42-43	0.27435433678516574
1104	44-45	0.23077700536700974
1104	46-47	0.3694371867674917
1104	48-49	0.413575813458543
1104	50-51	0.3856043034037029
1104	52-53	0.440312474841015
1104	54-55	0.4014074395991827
1104	56-57	0.4844613928027215
1104	58-59	0.21521965625256456
1104	60-61	0.3474843951968509
1104	62-63	0.14879254466871572
1104	64-65	0.25314953219564984
1104	66-67	0.35738715054627335
1104	68-69	0.5426584401779166
1104	70-71	0.44999574236690165
1104	72-73	0.30253140653805133
1104	74-75	0.15029961077043907
1104	76-77	0.27370695082188234
1104	78-79	0.287072200194487
1104	80-81	0.1994210906871956
1104	82-83	0.28313456894810685
1104	84-85	0.1985911944009473
1104	86-87	0.36774278749255274
1104	88-89	0.9507426269347903
1104	90-91	-0.5167960595287386
1104	92-93	-0.30470708970610527
1104	94-95	-0.10878272697584634
1104	96-97	0.0
1104	98-99	0.0
1104	100-101	0.0
1104	102-103	0.0
1104	104-105	0.0
1104	106-107	0.0
1104	108-109	0.0
1104	110-111	0.0
1104	112-113	0.0
1104	114-115	0.0
1104	116-117	0.0
1104	118-119	0.0
1104	120-121	0.0
1104	122-123	0.0
1104	124-125	0.0
1105	1	0.0998151221172705
1105	2	0.12357888797194505
1105	3	0.0606285003592788
1105	4	0.18481321795702854
1105	5	0.22146164820917136
1105	6	0.14701779230384204
1105	7	0.039545187792043635
1105	8	-0.03335312370695931
1105	9	0.12710542621164933
1105	10-11	0.008771480552020705
1105	12-13	0.04368409663184991
1105	14-15	-0.053866709404267965
1105	16-17	0.18539532068672315
1105	18-19	0.1532039249313044
1105	20-21	0.17600048083083664
1105	22-23	0.03148918818610014
1105	24-25	0.11470578060648506
1105	26-27	0.2686816660818465
1105	28-29	0.16595107627715322
1105	30-31	0.17970405529207767
1105	32-33	0.3128702503876539
1105	34-35	0.3028327310027663
1105	36-37	0.2551209988425427
1105	38-39	0.29848186373445174
1105	40-41	0.2948819669944598
1105	42-43	0.40375173465698566
1105	44-45	0.26816539822414853
1105	46-47	0.38917101137609933
1105	48-49	0.34505399655541424
1105	50-51	0.2895218287828598
1105	52-53	0.3125872730308359
1105	54-55	0.33607043953802673
1105	56-57	0.27581241255565914
1105	58-59	0.3116337034639187
1105	60-61	0.32297668971740023
1105	62-63	0.5880297174916151
1105	64-65	0.4564054412587666
1105	66-67	0.4551198437948045
1105	68-69	0.529948794568817
1105	70-71	0.36598938993294894
1105	72-73	0.5105764851217103
1105	74-75	0.5244988677420608
1105	76-77	0.5480044373678723
1105	78-79	0.46562356241317104
1105	80-81	0.19205196152867643
1105	82-83	0.25700053335612694
1105	84-85	0.3296818102277541
1105	86-87	0.141895987406933
1105	88-89	0.7723078406701127
1105	90-91	-0.766306822738132
1105	92-93	-0.30470708970610527
1105	94-95	-0.10878272697584634
1105	96-97	0.0
1105	98-99	0.0
1105	100-101	0.0
1105	102-103	0.0
1105	104-105	0.0
1105	106-107	0.0
1105	108-109	0.0
1105	110-111	0.0
1105	112-113	0.0
1105	114-115	0.0
1105	116-117	0.0
1105	118-119	0.0
1105	120-121	0.0
1105	122-123	0.0
1105	124-125	0.0
1106	1	-0.047563420847733795
1106	2	-0.03885912886650189
1106	3	-0.18598583329364615
1106	4	-0.051817332438467645
1106	5	0.024654810960846874
1106	6	-0.1708790497383461
1106	7	-0.2331751052328812
1106	8	-0.0797833804782897
1106	9	-0.26951845771692007
1106	10-11	-0.27633654991897316
1106	12-13	-0.2890699781524262
1106	14-15	-0.20555130312430236
1106	16-17	-0.22773321853307493
1106	18-19	-0.20686300190234874
1106	20-21	-0.1917750165325387
1106	22-23	-0.18775096566696448
1106	24-25	-0.12999100750472792
1106	26-27	-0.19089070628152882
1106	28-29	0.019076731688230097
1106	30-31	-0.014627668851019848
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2215	114-115	0.0
2215	116-117	0.0
2215	118-119	0.0
2215	120-121	0.0
2215	122-123	0.0
2215	124-125	0.0
2216	1	0.2907484456235565
2216	2	0.15312758097508805
2216	3	0.2322086890385222
2216	4	0.2691476814004261
2216	5	0.21219965921545736
2216	6	0.3170993567692477
2216	7	0.2833418662511633
2216	8	0.31211046709492507
2216	9	0.20678432754812803
2216	10-11	0.3468555754812641
2216	12-13	0.289360197260784
2216	14-15	0.25369520097309106
2216	16-17	0.22354574816313288
2216	18-19	0.2554113246954586
2216	20-21	0.2215242622773772
2216	22-23	0.18797565143295714
2216	24-25	0.07166332777629236
2216	26-27	0.05731423290574611
2216	28-29	0.10710857922526884
2216	30-31	0.017935555881699372
2216	32-33	-0.10396838954945054
2216	34-35	-0.03925281341547304
2216	36-37	-0.0488693952219208
2216	38-39	0.07667012533036655
2216	40-41	0.04358909651489995
2216	42-43	-0.018989273597732392
2216	44-45	0.0324391796706891
2216	46-47	-0.06143580859245645
2216	48-49	-0.10237745981250868
2216	50-51	-0.06609125592625986
2216	52-53	-0.059157518635835515
2216	54-55	-0.07472149737235156
2216	56-57	-0.07534165092705081
2216	58-59	0.025359627206057667
2216	60-61	-0.10258345179203587
2216	62-63	-0.11743534736687167
2216	64-65	-0.030847040069843956
2216	66-67	-0.10111541563915694
2216	68-69	-0.30076501734156125
2216	70-71	-0.2790239994145338
2216	72-73	-0.17642294982325524
2216	74-75	-0.2930927694434686
2216	76-77	-0.1825994344874715
2216	78-79	-0.1401492117613543
2216	80-81	-0.14511391897446657
2216	82-83	-0.047570270496073874
2216	84-85	-0.09299777900180572
2216	86-87	-0.3326427557141365
2216	88-89	-1.3088113368063024
2216	90-91	-0.766306822738132
2216	92-93	-0.30470708970610527
2216	94-95	-0.10878272697584634
2216	96-97	0.0
2216	98-99	0.0
2216	100-101	0.0
2216	102-103	0.0
2216	104-105	0.0
2216	106-107	0.0
2216	108-109	0.0
2216	110-111	0.0
2216	112-113	0.0
2216	114-115	0.0
2216	116-117	0.0
2216	118-119	0.0
2216	120-121	0.0
2216	122-123	0.0
2216	124-125	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9658.0
3	1472.0
4	1043.0
5	955.0
6	851.0
7	894.0
8	1050.0
9	1285.0
10	1486.0
11	1873.0
12	2240.0
13	2683.0
14	2939.0
15	3466.0
16	3953.0
17	4544.0
18	5805.0
19	7089.0
20	7357.0
21	10458.0
22	11623.0
23	14568.0
24	22295.0
25	37663.0
26	69738.0
27	190367.0
28	274902.0
29	10300.0
30	377.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.78694728096806	19.872278193969848	14.532801088011107	22.80797343705099
2	13.292485322172446	20.557494263306072	43.95139779011027	22.198622624411215
3	19.95107192057655	26.068789634875273	28.589055366434028	25.391083078114146
4	11.610004497298775	17.18580105999624	39.75526724771008	31.4489271949949
5	12.494075820952451	39.37184488542317	34.39211874260812	13.741960551016255
6	29.50618407987094	37.38003852424268	19.663012459206698	13.450764936679688
7	28.267518714417	32.29335897822555	21.70715885132886	17.731963456028588
8	24.601598443097075	37.38160339377523	20.537347745307525	17.479450417820168
9	25.797727809438726	18.759655956320223	21.669601982547437	33.77301425169362
10-11	24.269859210632074	27.19682414808313	28.85147088008115	19.681845761203647
12-13	26.233700275704695	24.663335827668913	28.44175631574305	20.661207580883335
14-15	21.12004825488595	28.385097320658836	26.45091857841561	24.0439358460396
16-17	20.534931213069836	30.36860632935073	28.71794762363047	20.37851483394897
18-19	21.078883983764243	29.324947040303435	29.329001711498055	20.267167264434274
20-21	22.492853083292612	28.33401263718559	27.918890408727528	21.254243870794273
22-23	21.20185252759337	29.64257541453186	28.202463540188482	20.95310851768629
24-25	21.877302847778026	28.609015924681408	29.102945653503742	20.410735574036824
26-27	20.50192478952505	29.32992286615813	29.304671562337287	20.863480781979533
28-29	20.14153654428334	30.300614003141146	27.808182345844813	21.7496671067307
30-31	21.17417851462584	28.64998705426114	28.510286883263582	21.665547547849442
32-33	20.519342691346328	29.40433359367331	29.15501894222302	20.921304772757342
34-35	21.157264520754808	29.50627619824635	29.003810460022176	20.332648820976665
36-37	20.638794423654584	28.741765757358273	29.272825950107656	21.346613868879487
38-39	21.077796777506848	29.68970059778016	28.45139088449271	20.781111740220275
40-41	22.251377796492985	28.542508969547637	28.888202875376635	20.31791035858274
42-43	20.305675924055457	29.837651899040306	28.722753487525143	21.13391868937909
44-45	20.50599381022529	29.43614154112729	28.576245121337934	21.48161952730948
46-47	21.827511544469324	28.79260357302392	28.30834758313014	21.071537299376615
48-49	21.28700652337668	28.934813890645415	28.432703804840713	21.34547578113719
50-51	20.050886711981494	30.046206329470476	28.892257310074633	21.010649648473397
52-53	20.470911920607055	29.67895990235214	28.751916965177386	21.09821121186342
54-55	20.29859600553688	28.724554467559038	29.209102561731516	21.76774696517257
56-57	21.125240776516716	28.87376339741711	27.926377156319088	22.07461866974709
58-59	21.820754153305998	29.14398791351677	27.52797559941616	21.50728233376106
60-61	20.796547044245976	28.431687754469127	29.776764248137095	20.995000953147805
62-63	20.85899956468175	28.58020809919566	29.42011625558018	21.140676080542413
64-65	20.941098980202252	29.5897833863374	28.115390716191502	21.353726917268844
66-67	21.112864081122837	29.823923725413763	27.484230382937803	21.578981810525597
68-69	21.47869315857946	28.475036329998016	28.97636540687108	21.069905104551438
70-71	22.30991814309736	28.441290362964377	28.231953497767925	21.016837996170338
72-73	21.54085589827779	28.908261657566715	28.671966358150268	20.87891608600523
74-75	20.58287122261834	30.06334876389533	28.87860026688139	20.475179746604944
76-77	21.00161608344453	28.51932044829244	29.147473304748384	21.33159016351464
78-79	21.08583837827726	28.66246568833521	28.724918430885516	21.52677750250202
80-81	21.59953850574876	29.13374513112184	28.41682149390981	20.849894869219586
82-83	21.142162905383827	28.910011601447938	29.54165389274458	20.406171600423654
84-85	20.817545329679124	29.182988148950077	29.334993993030633	20.664472528340163
86-87	20.299291817346347	30.137098357437342	28.12195024796351	21.441659577252796
88-89	29.99902192905549	18.58819424312729	34.51647608968999	16.89630773812723
90-91	30.482752100820072	14.93943290640052	16.5411785815232	38.03663641125621
92-93	22.422692097520468	17.519942921366546	35.142374442516896	24.914990538596086
94-95	15.299628072778223	6.833776349137664	27.112953576064786	50.753642002019326
96-97	26.23406323883598	7.147651192080782	14.750342488454823	51.86794308062841
98-99	57.052469925774254	14.1714870744817	18.765293063731765	10.010749936012285
100-101	30.911586003991616	6.922829743139227	12.482348744586702	49.68323550828245
102-103	22.98337576301316	1.9938154377240083	37.016290264551714	38.00651853471112
104-105	41.422752944242085	4.666035993678165	40.300853674425454	13.610357387654293
106-107	5.289324249792203	1.6790827384235343	6.3034544903713945	86.72813852141287
108-109	17.769674588850002	10.739683291772604	20.973799236845682	50.51684288253171
110-111	39.98732395352583	2.6920841241272084	24.560131849558907	32.76046007278806
112-113	27.23032815270493	2.763117413540182	38.48433549783228	31.52221893592261
114-115	27.889453983018537	3.902729816383195	23.0640752297224	45.143740970875875
116-117	19.134482004133854	4.09264424414813	19.85588861957697	56.91698513214104
118-119	19.78598660327779	4.189386920222165	15.158649391679452	60.865977084820585
120-121	24.97616847252643	4.157237143709568	29.461225559040333	41.405368824723666
122-123	33.984431416587825	1.5939965750295486	33.416746985465984	31.00482502291665
124-125	38.13815651817448	1.9245577901106112	19.511181394660316	40.42610429705459
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10.0
1	27.0
2	77.5
3	129.5
4	144.5
5	151.0
6	134.5
7	108.0
8	104.0
9	96.0
10	85.5
11	88.5
12	97.0
13	110.5
14	123.5
15	150.0
16	196.0
17	232.5
18	306.0
19	463.0
20	675.5
21	987.0
22	1408.5
23	1970.0
24	2773.0
25	3845.0
26	5177.0
27	6758.0
28	8489.0
29	10615.0
30	13246.5
31	16194.5
32	19511.0
33	23051.5
34	26295.0
35	28927.0
36	30162.5
37	30271.0
38	30129.5
39	29509.5
40	28722.5
41	27848.0
42	27254.0
43	27517.0
44	27865.5
45	27182.0
46	25683.5
47	24401.5
48	23435.0
49	22397.5
50	21162.0
51	19607.5
52	17526.5
53	15197.5
54	13160.0
55	11339.5
56	9667.5
57	8246.5
58	6929.5
59	5676.5
60	4552.0
61	3585.5
62	2772.5
63	2125.5
64	1627.0
65	1220.0
66	903.5
67	632.5
68	455.0
69	348.5
70	264.5
71	189.5
72	145.0
73	117.0
74	84.0
75	60.5
76	45.5
77	34.0
78	26.5
79	21.0
80	16.5
81	11.0
82	7.0
83	8.0
84	8.0
85	6.0
86	5.5
87	3.0
88	1.5
89	2.0
90	2.0
91	1.5
92	1.5
93	1.0
94	0.5
95	0.5
96	1.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.0
2	4.2678259978888485E-4
3	0.03841043398099964
4	0.04125565131292554
5	0.043389564311869966
6	0.0
7	0.0
8	0.0
9	0.0
10-11	6.401738996833274E-4
12-13	0.0011380869327703596
14-15	0.0
16-17	3.556521664907374E-4
18-19	0.005832695530448093
20-21	0.0012092173660685072
22-23	3.556521664907374E-4
24-25	0.0
26-27	0.0
28-29	8.535651995777697E-4
30-31	0.0
32-33	9.958260661740647E-4
34-35	7.113043329814748E-5
36-37	7.113043329814748E-5
38-39	0.0
40-41	0.0
42-43	0.0
44-45	7.113043329814748E-5
46-47	0.0
48-49	7.113043329814748E-5
50-51	0.0
52-53	0.0
54-55	0.0015648695325592445
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	7.113043329814748E-5
66-67	0.0
68-69	3.556521664907374E-4
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	3.556521664907374E-4
80-81	0.0
82-83	6.401738996833274E-4
84-85	7.113043329814748E-5
86-87	0.0011380869327703596
88-89	19.2749959455653
90-91	26.26484136490766
92-93	40.38259637462407
94-95	44.76750306572168
96-97	48.49359968361183
98-99	93.05233492760344
100-101	51.74354918100419
102-103	53.14375176047822
104-105	74.88654695888945
106-107	81.68661638219235
108-109	12.628852779919594
110-111	39.06013935874492
112-113	52.14152395530732
114-115	86.75529992858505
116-117	66.51556191619696
118-119	71.90191397769918
120-121	80.45008492973736
122-123	75.86736450363762
124-125	50.02724295595319
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	702934.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.6041509541433
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.90967458050322	69.88669482009632
2	8.046153319580924	12.649221002650402
3	1.5770670961626463	3.7189206027474317
4	0.5521113024260036	1.7359296063752898
5	0.25875243026444267	1.0169507544128844
6	0.14018732051348615	0.661158318209936
7	0.10579363965263874	0.5821073454870979
8	0.07041942142191915	0.4428207065241573
9	0.05198504370219924	0.3677616200272864
>10	0.2558514640695266	3.5577834487569957
>50	0.015369565504516201	0.8527794958153139
>100	0.013922617908306827	2.031985372566348
>500	0.0018081321934497064	1.028713024771963
>1k	9.040660967248532E-4	1.467173881558576
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	3987	0.567194075119428	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	2218	0.3155346021105822	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	1470	0.20912347389655359	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	1404	0.19973425670119813	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	1244	0.17697251804579092	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	885	0.12590086693772104	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	834	0.11864556274130998	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	833	0.1185033018747137	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	805	0.11451999761001744	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	745	0.10598434561423975	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	732	0.10413495434848791	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	1.4226086659629495E-4	0.0	0.0	0.0	0.0
14-15	1.4226086659629495E-4	0.0	0.0	0.0	0.0
16-17	1.4226086659629495E-4	0.0	0.0	0.0	0.0
18-19	1.4226086659629495E-4	0.0	0.0	0.0	0.0
20-21	1.4226086659629495E-4	0.0	0.0	0.0	0.0
22-23	1.4226086659629495E-4	0.0	0.0	2.845217331925899E-4	0.0
24-25	1.4226086659629495E-4	0.0	0.0	4.2678259978888485E-4	0.0
26-27	1.4226086659629495E-4	0.0	0.0	4.2678259978888485E-4	0.0
28-29	1.4226086659629495E-4	0.0	0.0	0.0011380869327703596	0.0
30-31	1.4226086659629495E-4	0.0	0.0	0.003983304264696259	0.0
32-33	1.4226086659629495E-4	0.0	0.0	0.00789547809609437	0.0
34-35	1.4226086659629495E-4	0.0	0.0	0.014012695359735053	0.0
36-37	1.4226086659629495E-4	0.0	0.0	0.0211257386895498	0.0
38-39	1.4226086659629495E-4	0.0	0.0	0.03464052101619782	0.0
40-41	1.4226086659629495E-4	0.0	0.0	0.05519721623936244	0.0
42-43	2.1339129989444243E-4	0.0	0.0	0.0761095636290178	0.0
44-45	2.845217331925899E-4	0.0	0.0	0.09979599791730091	0.0
46-47	2.845217331925899E-4	0.0	0.0	0.12518956260473957	0.0
48-49	4.2678259978888485E-4	0.0	0.0	0.1554911271897504	0.0
50-51	4.2678259978888485E-4	0.0	0.0	0.19397269160404818	0.0
52-53	4.2678259978888485E-4	0.0	0.0	0.23124503865227747	0.0
54-55	4.2678259978888485E-4	0.0	0.0	0.27321199429818444	0.0
56-57	4.2678259978888485E-4	0.0	0.0	0.31887773247559514	0.0
58-59	4.2678259978888485E-4	0.0	0.0	0.3711586009497335	0.0
60-61	4.2678259978888485E-4	0.0	0.0	0.43439355615178665	0.0
62-63	4.2678259978888485E-4	0.0	0.0	0.5015406851852379	0.0
64-65	4.2678259978888485E-4	0.0	0.0	0.564775640387291	0.0
66-67	4.2678259978888485E-4	0.0	0.0	0.6399605083834329	0.0
68-69	4.2678259978888485E-4	0.0	0.0	0.7318610282046394	0.0
70-71	4.2678259978888485E-4	0.0	0.0	0.8399081563845254	0.0
72-73	4.2678259978888485E-4	0.0	0.0	0.9367878065366022	0.0
74-75	4.2678259978888485E-4	0.0	0.0	1.0388599783194439	0.0
76-77	4.2678259978888485E-4	0.0	0.0	1.1471204977992244	0.0
78-79	4.2678259978888485E-4	0.0	0.0	1.177777714550726	0.0
80-81	4.2678259978888485E-4	0.0	0.0	1.1780622362839186	0.0
82-83	4.2678259978888485E-4	0.0	0.0	1.1780622362839186	0.0
84-85	4.2678259978888485E-4	0.0	0.0	1.1780622362839186	0.0
86-87	4.2678259978888485E-4	0.0	0.0	1.1780622362839186	0.0
88-89	4.2678259978888485E-4	0.0	0.0	1.1780622362839186	0.0
90-91	4.2678259978888485E-4	0.0	0.0	1.1780622362839186	0.0
92-93	4.2678259978888485E-4	0.0	0.0	1.1780622362839186	0.0
94-95	4.2678259978888485E-4	0.0	0.0	1.1780622362839186	0.0
96-97	4.2678259978888485E-4	0.0	0.0	1.1780622362839186	0.0
98-99	4.2678259978888485E-4	0.0	0.0	1.1780622362839186	0.0
100-101	4.2678259978888485E-4	0.0	0.0	1.1780622362839186	0.0
102-103	4.2678259978888485E-4	0.0	0.0	1.1780622362839186	0.0
104-105	4.2678259978888485E-4	0.0	0.0	1.1780622362839186	0.0
106-107	4.2678259978888485E-4	0.0	0.0	1.1780622362839186	0.0
108-109	4.2678259978888485E-4	0.0	0.0	1.1780622362839186	0.0
110-111	4.2678259978888485E-4	0.0	0.0	1.1780622362839186	0.0
112-113	4.2678259978888485E-4	0.0	0.0	1.1780622362839186	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCGCT	25	2.701814E-6	1998.2607	108-109
CCGCTCG	75	0.0	1998.2605	108-109
CGTACGT	15	0.0020645114	1986.7194	110-111
TGTCGCG	15	0.0020645114	1986.7194	110-111
CCGCTCC	255	0.0	1763.1711	108-109
CGTGTCG	15	0.0029408305	1665.217	108-109
CCGTTCG	15	0.0029408305	1665.217	108-109
CCGCGCC	195	0.0	1537.1237	108-109
CGTCGCG	20	0.0036698359	1490.0397	110-111
CGCCGCT	20	0.005227451	1248.9128	108-109
CGTCGTG	50	1.2690542E-5	1192.0317	110-111
CTCCGCC	140	0.0	1064.314	110-111
CCGGACG	25	0.0081668105	999.1304	108-109
CGGTGCG	25	0.0081668105	999.1304	108-109
CCGTTAC	25	0.0081668105	999.1304	108-109
GTCGACT	30	0.008255303	993.3597	110-111
CGCTATT	30	0.008255303	993.3597	110-111
GACGGTA	30	0.008255303	993.3597	110-111
CGCGTCT	30	0.008255303	993.3597	110-111
TTGTTCG	105	1.70985E-10	951.5527	108-109
>>END_MODULE