FastQCFastQC Report
Fri 27 May 2016
SRR939964_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939964_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences702934
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44250.6295043346886052No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23680.33687373210002647No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21790.30998642831332673No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16220.23074712561919042No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC13870.1973158219690611No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT13540.19262121337138338No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT13050.18565043090816494No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10170.14467930132843196No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC9620.13685495366563574No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT9550.13585912759946167No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC9050.12874608426964693No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG8840.12575860607112474No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA8470.12049495400706182No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC7600.10811825861318416No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA7350.10456173694827679No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA7100.10100521528336942No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG300.0042124759.5832823
GTACCTG8500.034.355351
TACCTGG8350.034.2514652
GTACACG1251.261089E-633.373771
GTACATG40100.032.3988341
TACATGG39500.032.280562
ACATGGG38900.031.8594423
TAGGACC9400.029.7916414
CATGGGC9450.028.3729924
GAGTACT30900.028.20683312-13
GTACTTT31850.027.17966814-15
CATGGGG19550.027.1248684
TGCACCG900.00946175226.4814575
AACTCGC450.00953414326.44189854-55
AGTACTT31150.026.07052412-13
GTGTAGC6250.025.7454781
GTAGGAC22350.025.3262253
TACACGG1651.0970649E-525.2777562
GCACCGT1200.001288087124.7892826
AGAGTAC52350.023.80907210-11
Produced by FastQC (version 0.11.5)