##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939964_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 702934 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.81573661254115 34.0 31.0 34.0 31.0 34.0 2 32.88502619022554 34.0 31.0 34.0 31.0 34.0 3 32.93694856131586 34.0 31.0 34.0 31.0 34.0 4 36.34450602759292 37.0 37.0 37.0 35.0 37.0 5 36.25118432171441 37.0 37.0 37.0 35.0 37.0 6 36.39172525443356 37.0 37.0 37.0 35.0 37.0 7 36.394149379600364 37.0 37.0 37.0 35.0 37.0 8 36.41549846785047 37.0 37.0 37.0 35.0 37.0 9 38.245374387922624 39.0 39.0 39.0 37.0 39.0 10-11 38.243133779273734 39.0 39.0 39.0 37.0 39.0 12-13 38.23221739167546 39.0 39.0 39.0 37.0 39.0 14-15 39.74930434436234 41.0 40.0 41.0 37.0 41.0 16-17 39.78291987583472 41.0 40.0 41.0 37.5 41.0 18-19 39.8299242887668 41.0 40.0 41.0 37.5 41.0 20-21 39.85008265356349 41.0 40.0 41.0 38.0 41.0 22-23 39.80088671198149 41.0 40.0 41.0 37.5 41.0 24-25 39.76106647281252 41.0 40.0 41.0 37.0 41.0 26-27 39.65790813931322 41.0 40.0 41.0 37.0 41.0 28-29 39.58047981745085 41.0 40.0 41.0 37.0 41.0 30-31 39.45362366879394 41.0 39.5 41.0 36.5 41.0 32-33 39.290523007849956 41.0 39.0 41.0 35.0 41.0 34-35 39.177645411944795 41.0 39.0 41.0 35.0 41.0 36-37 39.161666671408696 41.0 39.0 41.0 35.0 41.0 38-39 39.211047552117265 41.0 39.0 41.0 35.0 41.0 40-41 39.141353953571745 41.0 39.0 41.0 35.0 41.0 42-43 39.032808201054436 41.0 39.0 41.0 35.0 41.0 44-45 38.85984672814233 41.0 39.0 41.0 35.0 41.0 46-47 38.60923002728563 41.0 39.0 41.0 35.0 41.0 48-49 38.34086912853839 41.0 39.0 41.0 35.0 41.0 50-51 38.17026207296844 41.0 39.0 41.0 35.0 41.0 52-53 38.07477942452634 41.0 39.0 41.0 34.0 41.0 54-55 37.96485374160305 41.0 38.5 41.0 34.0 41.0 56-57 37.80947713441091 41.0 38.0 41.0 34.0 41.0 58-59 37.650939490763 40.0 38.0 41.0 34.0 41.0 60-61 37.45119954362714 40.0 37.0 41.0 33.0 41.0 62-63 37.256394625953504 40.0 37.0 41.0 33.0 41.0 64-65 37.01945132828971 39.5 36.5 41.0 33.0 41.0 66-67 36.746129081819916 39.0 36.0 41.0 32.5 41.0 68-69 36.409619537538376 39.0 35.5 41.0 32.0 41.0 70-71 36.040527275675956 38.5 35.0 40.5 32.0 41.0 72-73 35.69050010385043 37.0 35.0 40.0 31.5 41.0 74-75 35.34087979810338 37.0 35.0 39.0 31.0 41.0 76-77 34.961724002537935 36.0 35.0 39.0 31.0 41.0 78-79 34.59041602767827 36.0 35.0 38.5 31.0 40.0 80-81 34.28709380966065 35.5 35.0 37.0 31.0 39.0 82-83 34.03290138192206 35.0 35.0 37.0 31.0 39.0 84-85 33.75238855995015 35.0 35.0 36.5 31.0 38.5 86-87 33.53973772786634 35.0 35.0 36.0 31.0 37.0 88-89 33.323262923688425 35.0 35.0 36.0 31.0 37.0 90-91 33.13721345104946 35.0 34.5 36.0 30.0 37.0 92-93 32.98650797941201 35.0 34.0 35.0 30.0 36.0 94-95 32.87649907388176 35.0 34.0 35.0 30.0 36.0 96-97 32.77706726378294 35.0 34.0 35.0 30.0 36.0 98-99 32.68587306916439 35.0 34.0 35.0 29.5 36.0 100-101 32.65048781251156 35.0 34.0 35.0 30.0 36.0 102-103 31.80647116229973 34.5 32.5 35.0 27.5 35.0 104-105 32.471604019723046 35.0 34.0 35.0 29.0 35.0 106-107 32.52309889690924 35.0 34.0 35.0 29.0 35.0 108-109 32.536771588797805 35.0 34.0 35.0 29.0 35.0 110-111 32.52472067078844 35.0 34.0 35.0 29.5 35.0 112-113 32.457558604363996 35.0 34.0 35.0 29.0 35.0 114-115 32.39339255179007 35.0 34.0 35.0 29.0 35.0 116-117 32.33024722093397 35.0 34.0 35.0 29.0 35.0 118-119 32.25558018249224 35.0 34.0 35.0 29.0 35.0 120-121 32.174248933754804 35.0 34.0 35.0 29.0 35.0 122-123 32.014688434476064 35.0 34.0 35.0 29.0 35.0 124-125 30.008135187656308 33.5 30.5 34.5 22.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09406665695595251 1101 2 0.09695214381744677 1101 3 0.1301743737166987 1101 4 0.07974339991696411 1101 5 -0.021547439595487106 1101 6 -0.020791107942443432 1101 7 -0.018236009666559028 1101 8 0.04804576971577035 1101 9 0.10921117615837517 1101 10-11 0.08909082766437848 1101 12-13 0.03867269391787431 1101 14-15 0.08257432683936372 1101 16-17 0.15983789221571953 1101 18-19 0.13687732750048553 1101 20-21 0.12872291893463483 1101 22-23 0.1532225656066757 1101 24-25 0.14865886867982425 1101 26-27 0.18461269093064914 1101 28-29 0.11355745830743302 1101 30-31 0.048303238762507306 1101 32-33 0.0536575937019137 1101 34-35 0.15885492485114128 1101 36-37 0.08816546968983374 1101 38-39 -0.0247610930910227 1101 40-41 0.12685330402881334 1101 42-43 0.021546331429021848 1101 44-45 -0.03795742940170044 1101 46-47 0.010926132875347605 1101 48-49 -0.034026563784635755 1101 50-51 0.05944392379060304 1101 52-53 0.0027835591455627196 1101 54-55 -0.09571017953406624 1101 56-57 -1.1520935629505402E-5 1101 58-59 0.09386113216133651 1101 60-61 0.02573819726850246 1101 62-63 -0.03739364433437231 1101 64-65 -0.022545018462771793 1101 66-67 0.05062281136900282 1101 68-69 -0.19183197090190163 1101 70-71 -0.14502036901092907 1101 72-73 -0.036665368714409396 1101 74-75 -0.14370018547342767 1101 76-77 -0.073883656701625 1101 78-79 -0.029595473603762912 1101 80-81 -0.08929444928833874 1101 82-83 -0.21764349481976808 1101 84-85 -0.23169406176212703 1101 86-87 -0.16289400666983056 1101 88-89 -0.14034588138186876 1101 90-91 -0.05942540168295807 1101 92-93 -0.09730509507313911 1101 94-95 -0.04581247340153283 1101 96-97 -0.07257032657955875 1101 98-99 -0.009440839515406196 1101 100-101 -0.08558519434239287 1101 102-103 -0.028280945614966413 1101 104-105 0.0366946266911512 1101 106-107 -0.0406046129331088 1101 108-109 -0.030878607852280027 1101 110-111 -0.028054356232189548 1101 112-113 0.0045363017547543905 1101 114-115 0.018282794568861505 1101 116-117 -0.09655582978822252 1101 118-119 -0.05049260401492717 1101 120-121 0.06212831794698559 1101 122-123 0.012791628994449411 1101 124-125 0.09431754978107776 1102 1 0.07891582167211197 1102 2 0.08310048822423965 1102 3 0.11727127582533825 1102 4 0.08330179569614415 1102 5 -0.006267208101498056 1102 6 0.053746326694209756 1102 7 -0.05684095155826441 1102 8 0.03238882828738099 1102 9 0.004307372848963098 1102 10-11 0.07139015657806169 1102 12-13 0.05247165186096936 1102 14-15 0.04804849612090578 1102 16-17 0.21014327770685526 1102 18-19 0.017852119240416187 1102 20-21 0.03411968006401622 1102 22-23 0.1298686367098938 1102 24-25 0.12891707673013997 1102 26-27 0.033942282904746435 1102 28-29 0.13255000445960974 1102 30-31 0.1179173563064353 1102 32-33 0.16840072657199556 1102 34-35 0.11963795975577085 1102 36-37 0.28564756553276993 1102 38-39 0.22722550396604646 1102 40-41 0.20485052201028964 1102 42-43 0.09820628131929254 1102 44-45 0.09582572968154324 1102 46-47 -0.009716736715191132 1102 48-49 0.06916743557047056 1102 50-51 0.10433449529400463 1102 52-53 0.1028438744757878 1102 54-55 0.011481014845124093 1102 56-57 -0.021981223439141218 1102 58-59 0.027781409486635766 1102 60-61 0.05075758061207836 1102 62-63 -0.1056866683133677 1102 64-65 -0.02030510745397862 1102 66-67 0.08725133050242562 1102 68-69 -0.09666627592424959 1102 70-71 0.004994188062703131 1102 72-73 -0.011591722443995423 1102 74-75 0.08676646526220821 1102 76-77 0.09971909902024123 1102 78-79 0.11266731131573948 1102 80-81 0.16226274018423226 1102 82-83 0.0892436184655736 1102 84-85 0.117398129907059 1102 86-87 0.16423578712061726 1102 88-89 0.028187464751951552 1102 90-91 0.13362435220336266 1102 92-93 0.11742843743296305 1102 94-95 0.01443294112026905 1102 96-97 0.11153230548544713 1102 98-99 0.14268058004450523 1102 100-101 0.07338118038277486 1102 102-103 0.1230787059335583 1102 104-105 0.15677535322999603 1102 106-107 0.23791081599968322 1102 108-109 0.005219282467741948 1102 110-111 0.09339859624496682 1102 112-113 0.06538695661436833 1102 114-115 0.19950740447580984 1102 116-117 0.1523607378312093 1102 118-119 0.16423675441988195 1102 120-121 0.3558864117321008 1102 122-123 0.39932002259268273 1102 124-125 0.48282862724453324 1103 1 0.059965066858993055 1103 2 0.07169386824841695 1103 3 0.06957867609109769 1103 4 0.10472829757942748 1103 5 -0.002234877522319323 1103 6 0.10954155303823399 1103 7 0.08613394128924057 1103 8 0.08999340811973155 1103 9 0.1378718291936849 1103 10-11 0.09611315864617609 1103 12-13 0.13498106829202072 1103 14-15 0.1274072796279171 1103 16-17 0.15679182752651855 1103 18-19 0.14527166745154574 1103 20-21 0.12916319046954783 1103 22-23 0.10276130438231235 1103 24-25 0.2075385312065734 1103 26-27 0.19214082223395934 1103 28-29 0.22159497353775492 1103 30-31 0.07996439350258555 1103 32-33 0.179051534336935 1103 34-35 0.15787916026331317 1103 36-37 0.06798413925069013 1103 38-39 0.019624420951586785 1103 40-41 -0.03761883087857143 1103 42-43 -0.03770653801511514 1103 44-45 0.12561112497182592 1103 46-47 0.148646652600533 1103 48-49 0.01749595661631531 1103 50-51 0.042134597201268775 1103 52-53 0.020208073055080433 1103 54-55 0.0352608751722272 1103 56-57 0.011345948834090791 1103 58-59 -0.011413423215607565 1103 60-61 0.11675102657625303 1103 62-63 0.08648545505437255 1103 64-65 0.11932582839440897 1103 66-67 0.13774538091654875 1103 68-69 0.056282540652667024 1103 70-71 0.1912861623660902 1103 72-73 0.1235847669506569 1103 74-75 0.09802322161392141 1103 76-77 0.16220273890794346 1103 78-79 0.24236178934073394 1103 80-81 0.02254987888801452 1103 82-83 0.14491345891076435 1103 84-85 0.0632673888813855 1103 86-87 0.06839650087110272 1103 88-89 0.026845186225408213 1103 90-91 -0.08222122831836032 1103 92-93 -0.13996433514398632 1103 94-95 -0.10529110999321745 1103 96-97 -0.030154259740193368 1103 98-99 -0.06212854365293197 1103 100-101 0.04944096597788672 1103 102-103 -0.06803388161177537 1103 104-105 -0.10092913568508521 1103 106-107 -0.10158324440534727 1103 108-109 -0.09623589332229443 1103 110-111 -0.0011783387441965942 1103 112-113 0.08110515305560284 1103 114-115 -0.04345300155469545 1103 116-117 -0.028771931439756315 1103 118-119 -0.06378624465096294 1103 120-121 -0.05633646544966098 1103 122-123 -0.04058566323321955 1103 124-125 -0.05821452348829936 1104 1 0.058401446700500514 1104 2 0.11419837310203462 1104 3 0.11246875766285314 1104 4 -0.0014719310540058927 1104 5 0.00543596784009992 1104 6 -0.015830945072629277 1104 7 0.041240410840309494 1104 8 0.036900075303087476 1104 9 -0.1104880165756228 1104 10-11 0.05491457694847668 1104 12-13 0.013094261216011205 1104 14-15 0.006423380846648286 1104 16-17 -0.0059157348148417555 1104 18-19 0.03242653753738267 1104 20-21 0.22468061892472235 1104 22-23 0.12921302978968896 1104 24-25 0.1094576957257587 1104 26-27 0.12438394869924707 1104 28-29 0.12508260214406164 1104 30-31 0.21901905239313635 1104 32-33 0.22391503242842248 1104 34-35 0.2125070496667334 1104 36-37 0.19650131582157115 1104 38-39 0.1613335527258215 1104 40-41 0.08113976709472581 1104 42-43 0.09311150465021001 1104 44-45 0.05743767357580509 1104 46-47 0.029878366769992226 1104 48-49 -0.0424215028399999 1104 50-51 -0.06285464927719886 1104 52-53 -0.13622950808072432 1104 54-55 -0.0977708428723787 1104 56-57 -0.07551537917737505 1104 58-59 -0.10336959534945578 1104 60-61 -0.12120890444620613 1104 62-63 -0.054244234352935905 1104 64-65 -0.028758882229297456 1104 66-67 0.030215009484102495 1104 68-69 0.0921702390115442 1104 70-71 0.08467807494286461 1104 72-73 0.008615089943816656 1104 74-75 -0.03842927882202929 1104 76-77 -0.013739405208617939 1104 78-79 -0.01671088896426909 1104 80-81 -0.05812090834726291 1104 82-83 -0.032961412725121875 1104 84-85 -0.053233938355049304 1104 86-87 -0.06803468627892073 1104 88-89 -0.07980649655539196 1104 90-91 -0.024070834022573706 1104 92-93 -0.07571543471578934 1104 94-95 -0.14387687659336024 1104 96-97 -0.14540488460698242 1104 98-99 -0.1191163950799563 1104 100-101 -0.01010157340325435 1104 102-103 0.03101283323583459 1104 104-105 0.04892554645631719 1104 106-107 0.0828454626532249 1104 108-109 0.03871595802722538 1104 110-111 -0.010613342909969958 1104 112-113 -0.0311916441387865 1104 114-115 -0.026292900025950416 1104 116-117 -0.054188024908192745 1104 118-119 -0.18921880747672049 1104 120-121 -0.20141600275759686 1104 122-123 -0.1617546000877681 1104 124-125 -0.08528142783039172 1105 1 0.11917956252730733 1105 2 0.15031971911475495 1105 3 0.04595166495248293 1105 4 -0.0060719035344334316 1105 5 -0.028296526655985588 1105 6 0.019548901429324417 1105 7 -0.023105693611746858 1105 8 0.05649247990191242 1105 9 0.08061819367388523 1105 10-11 0.08411207572538615 1105 12-13 0.11610440523509169 1105 14-15 0.10893091050662917 1105 16-17 0.03455714312057978 1105 18-19 0.089803884345109 1105 20-21 0.1341813680685604 1105 22-23 0.149837418243699 1105 24-25 0.10822695942439253 1105 26-27 0.09648120744435573 1105 28-29 0.10563639525805257 1105 30-31 0.018489300680805343 1105 32-33 0.027595392629010007 1105 34-35 0.023733314038061337 1105 36-37 0.017107166787816652 1105 38-39 0.014137299973867812 1105 40-41 -0.03646339306916957 1105 42-43 0.036247283148256315 1105 44-45 -0.11718068350589306 1105 46-47 -0.18031954480045442 1105 48-49 -0.1455329341633842 1105 50-51 -0.1387073737145741 1105 52-53 -0.06673112867759556 1105 54-55 -0.1272894032931191 1105 56-57 -0.09402611180946963 1105 58-59 -0.07169246869828072 1105 60-61 -0.04566195300295561 1105 62-63 -0.015529551133752761 1105 64-65 0.10168292152559388 1105 66-67 -0.01423578399681702 1105 68-69 -0.049391190782976935 1105 70-71 0.18458462307153667 1105 72-73 0.10543810853481261 1105 74-75 0.037202715735212166 1105 76-77 0.09576554831388506 1105 78-79 0.15066160369717352 1105 80-81 -4.186777332719771E-4 1105 82-83 0.05938012042066987 1105 84-85 0.053347060911093536 1105 86-87 -0.059130958906123965 1105 88-89 -0.04069813060627325 1105 90-91 0.040664175456392115 1105 92-93 -0.06914131534200862 1105 94-95 -0.04711119043876266 1105 96-97 -0.10364107131197642 1105 98-99 -0.10065248393758708 1105 100-101 0.004768549517486065 1105 102-103 -0.05801106292365432 1105 104-105 0.0816165016911512 1105 106-107 0.1339152859164301 1105 108-109 0.11379756119503526 1105 110-111 0.005495160636996843 1105 112-113 -0.05054707466716479 1105 114-115 -0.014764302606678825 1105 116-117 -0.0161297980857924 1105 118-119 0.18518111302230267 1105 120-121 -0.13444502825906568 1105 122-123 -0.12722996242876405 1105 124-125 -0.30823676204027706 1106 1 0.023393106690775767 1106 2 0.1092863043856056 1106 3 0.06626379043186859 1106 4 0.03835654055713178 1106 5 -0.0153615209632747 1106 6 0.05598507562635291 1106 7 -0.05401300725029046 1106 8 -0.021434024172869215 1106 9 -0.01962131225852204 1106 10-11 0.0718671242632638 1106 12-13 0.1445085167223823 1106 14-15 -0.1676792472414519 1106 16-17 -0.046505971093225185 1106 18-19 0.06758591709319006 1106 20-21 0.05708910866059824 1106 22-23 0.08870816368952461 1106 24-25 0.10707718114059617 1106 26-27 0.07020343674085439 1106 28-29 0.189477984274113 1106 30-31 0.15420995324551967 1106 32-33 0.16223836212805054 1106 34-35 0.023201962917489993 1106 36-37 -0.03345639310551718 1106 38-39 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28-29 -0.15527469456112897 2216 30-31 -0.19749684318711758 2216 32-33 -0.251646205248349 2216 34-35 -0.11653238466477234 2216 36-37 -0.09748537939929491 2216 38-39 -0.05888684998682692 2216 40-41 -0.12185119279401135 2216 42-43 -0.16052084774797493 2216 44-45 -0.15730266168985452 2216 46-47 -0.02855927553945037 2216 48-49 0.0839438985803298 2216 50-51 0.04158817463448372 2216 52-53 0.03577722430982533 2216 54-55 -0.04539327514846292 2216 56-57 -0.14420545536292195 2216 58-59 -0.01832854869041256 2216 60-61 -0.1667986963205621 2216 62-63 -0.10732642613375276 2216 64-65 -0.1548981270200045 2216 66-67 -0.1283554036902146 2216 68-69 -0.18170005477669093 2216 70-71 -0.2289711699709187 2216 72-73 -0.17586102668531112 2216 74-75 -0.10848014098648662 2216 76-77 -0.19798936520813015 2216 78-79 -0.16947892303238632 2216 80-81 -0.15333669561849206 2216 82-83 0.03285324345526419 2216 84-85 0.10428800037649921 2216 86-87 0.03548504934859409 2216 88-89 0.0018529700226537216 2216 90-91 0.0895936418479053 2216 92-93 0.06342550855735851 2216 94-95 0.14072047229708318 2216 96-97 0.08200714507953677 2216 98-99 -0.00875426754607389 2216 100-101 -0.0400734807498111 2216 102-103 0.10840378573986698 2216 104-105 -0.019516065132748395 2216 106-107 -0.14597735362760034 2216 108-109 -0.16887428923735825 2216 110-111 -0.10897147654853256 2216 112-113 -0.09408087969860901 2216 114-115 -0.1220786943126484 2216 116-117 -0.44258972831378074 2216 118-119 -0.42373938619153506 2216 120-121 -0.3847613362386255 2216 122-123 -0.4686306847165014 2216 124-125 -0.6417144300827289 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50.0 3 4.0 4 11.0 5 20.0 6 55.0 7 152.0 8 271.0 9 475.0 10 1012.0 11 1611.0 12 1983.0 13 3300.0 14 5774.0 15 6372.0 16 2356.0 17 1538.0 18 1269.0 19 1149.0 20 1139.0 21 1069.0 22 1195.0 23 1258.0 24 1536.0 25 1882.0 26 2502.0 27 3154.0 28 4177.0 29 5249.0 30 6484.0 31 8656.0 32 10999.0 33 15217.0 34 21595.0 35 36304.0 36 80795.0 37 249582.0 38 219890.0 39 2849.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.998571485484355 19.68931232107998 14.956433152919349 22.355683040516315 2 13.6115197159335 20.838798521625073 43.36850970361371 22.181172058827713 3 20.03843888615432 26.260075625876684 28.828026528806404 24.873458959162594 4 11.492259586248496 17.407324158455843 39.59119917374888 31.509217081546776 5 12.11075375109321 38.93017995561646 34.45806780597725 14.500998487313085 6 29.094481131941265 37.80824373269752 19.131241339869746 13.96603379549147 7 27.428321862365458 32.82114679329781 21.753109111239464 17.997422233097275 8 24.872605393963017 36.50314254254311 20.259370011978366 18.364882051515504 9 25.921921545977288 17.960149886049045 21.916709107825202 34.201219460148465 10-11 24.20362366879394 27.010146045005644 28.614848620211852 20.171381665988555 12-13 26.717728833716965 24.401793055962578 28.707389313932747 20.173088796387713 14-15 21.367368771463607 27.728634551750236 27.57278777239399 23.331208904392163 16-17 19.890572863578136 29.821526502862643 29.656930563132928 20.63097007042629 18-19 20.28952931569678 28.032005849766833 31.77787672811388 19.90058810642251 20-21 21.659556881482477 27.006513687136728 30.659143596320693 20.674785835060096 22-23 20.474043345617606 28.262640100777958 30.904007004949985 20.359309548654455 24-25 21.100202863995765 27.251420474752962 31.942685942065687 19.705690719185583 26-27 19.8395581946527 27.974674720528526 32.05030628764578 20.135460797172993 28-29 19.449407768012357 28.893608788307297 30.63843831711085 21.018545126569492 30-31 20.47951870303613 27.226453692665313 31.30549952058088 20.988528083717675 32-33 19.861822020275017 28.08969263117163 31.85989011770664 20.18859523084671 34-35 20.428802704094554 28.1062660221301 31.73548298986818 19.729448283907168 36-37 20.039790364386985 27.532101164547456 31.82745464012269 20.60065383094288 38-39 20.469844964107583 28.486529318541997 31.015500744024333 20.028124973326086 40-41 21.663271373983903 27.30128290849497 31.25371656513983 19.781729152381306 42-43 19.89368845439259 28.566835577735606 31.010521613693463 20.528954354178346 44-45 20.189946709079372 28.2059197591808 30.58857588336885 21.015557648370972 46-47 21.594345984117997 27.825300810602414 29.984891895967476 20.595461309312114 48-49 21.25875259981734 28.14730828214313 29.659541294061746 20.934397823977786 50-51 20.097477145791782 29.237737824603734 29.986741287233226 20.678043742371262 52-53 20.496376615727794 29.086443392978573 29.69403955421135 20.723140437082286 54-55 20.346931575368384 28.011235763243775 30.166701283477536 21.4751313779103 56-57 21.020750170001737 28.168220629532787 28.98892356892681 21.822105631538665 58-59 21.720744764088806 28.423721145939734 28.514270187528272 21.34126390244319 60-61 20.75529139293305 27.718605160655198 30.809933791792687 20.71616965461907 62-63 20.748889653936217 27.849627418790384 30.419498843419156 20.981984083854247 64-65 20.73530374117627 28.94553400461494 29.244993128800147 21.074169125408645 66-67 20.994218518381526 29.04554339383214 28.562781143037615 21.397456944748726 68-69 21.25974842588351 27.8489872448907 30.028210329846043 20.863053999379744 70-71 22.095673278003343 27.689086030836464 29.398136951691058 20.81710373946914 72-73 21.33578685907923 28.12042097835643 29.85906215946305 20.684730003101286 74-75 20.49836826786014 29.31775956206415 29.81681068208395 20.367061487991762 76-77 20.86433434717911 27.77373124646126 30.148847544719704 21.213086861639926 78-79 20.746755740937274 27.942666025544362 29.88097033291888 21.429607900599486 80-81 21.41218094444144 28.242480801896054 29.48349347164883 20.861844782013677 82-83 20.9414397368743 28.18586097699073 30.541416406092182 20.331282880042792 84-85 20.64304756918857 28.378624451228706 30.298505976379005 20.679822003203714 86-87 19.975559583118756 29.344789126717448 29.116247044530496 21.563404245633304 88-89 20.594180961512745 28.448830188893982 29.269035215254917 21.68795363433836 90-91 20.896986406214545 28.19577725567854 29.800770060809622 21.10646627729729 92-93 20.61281713503686 28.991697655825437 29.950393635817875 20.44509157331983 94-95 20.348826870480952 28.897859309815022 29.70818469899101 21.045129120713018 96-97 20.58671226601644 28.419382189508546 29.993569808829847 21.000335735645166 98-99 20.975858887697843 28.350922816584024 29.76279982558706 20.910418470131074 100-101 20.294736273061392 28.11816480506335 30.11344600876185 21.4736529131134 102-103 20.37946717539225 28.374325860359377 29.717910526323276 21.528296437925093 104-105 21.101543911313126 27.877184184600722 29.460004481290614 21.561267422795545 106-107 20.58097118760777 27.808183903074724 29.813826306175063 21.797018603142444 108-109 20.096054878747076 28.449784936370044 29.435513986782496 22.018646198100384 110-111 19.7224956893363 29.079836906110046 29.64164584838983 21.556021556163824 112-113 20.35250482298644 27.89308050830578 29.156802544943407 22.597612123764378 114-115 19.974093245820384 28.216524127354248 28.639411699012175 23.1699709278132 116-117 20.733109127017997 28.04108267750141 28.266925016075707 22.958883179404886 118-119 20.402385719823737 27.40120850864059 29.65583466406803 22.540571107467645 120-121 19.754072590339593 28.229299888609265 29.546214351052946 22.470413169998192 122-123 20.293543577720623 28.050027883367857 28.92344592902829 22.73298260988323 124-125 20.5067652636326 28.14480552089898 28.661287270833895 22.687141944634522 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 89.0 1 62.0 2 84.5 3 117.0 4 62.5 5 36.5 6 106.0 7 307.0 8 537.5 9 571.0 10 426.5 11 324.5 12 326.0 13 362.5 14 465.0 15 574.5 16 641.5 17 748.0 18 900.5 19 1070.5 20 1295.0 21 1539.0 22 1923.5 23 2435.0 24 3010.5 25 3799.5 26 4691.5 27 5778.5 28 7201.5 29 8789.0 30 10709.5 31 13363.0 32 16572.0 33 20225.0 34 24417.5 35 28342.0 36 31605.0 37 32748.0 38 31264.0 39 28516.5 40 26119.0 41 24889.5 42 23825.5 43 22922.5 44 22516.0 45 22420.5 46 22256.5 47 24854.0 48 30552.0 49 29036.5 50 22668.5 51 19590.0 52 17557.0 53 16204.0 54 15033.0 55 13470.5 56 11833.5 57 10219.5 58 8840.0 59 7521.5 60 5929.5 61 4664.0 62 3788.0 63 2822.0 64 1948.5 65 1439.5 66 1057.5 67 822.0 68 616.5 69 405.0 70 298.0 71 226.5 72 173.5 73 142.0 74 103.5 75 79.0 76 48.0 77 19.0 78 11.0 79 5.0 80 2.0 81 0.0 82 1.0 83 1.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 1.0 98 3.0 99 3.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015079651859207266 2 0.0 3 0.0 4 0.0 5 0.1240514756719692 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.113043329814748E-5 18-19 0.0 20-21 0.004196695564590701 22-23 3.556521664907374E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 3.556521664907374E-4 92-93 0.0 94-95 9.246956328759172E-4 96-97 0.0 98-99 6.401738996833274E-4 100-101 7.113043329814748E-5 102-103 1.4226086659629495E-4 104-105 0.001635999965857392 106-107 0.005974956397044388 108-109 3.556521664907374E-4 110-111 0.0036987825315036686 112-113 0.007113043329814748 114-115 0.004054434697994406 116-117 0.0014226086659629496 118-119 2.1339129989444243E-4 120-121 4.2678259978888485E-4 122-123 8.535651995777697E-4 124-125 7.113043329814748E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 702934.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.05008224586473 #Duplication Level Percentage of deduplicated Percentage of total 1 88.20756512728396 67.08192582637416 2 8.455529746175113 12.860874652579463 3 1.6703357520665005 3.8108751396859706 4 0.6145321402517407 1.8694087923548868 5 0.2914357655452807 1.1081856969552575 6 0.16962659963038826 0.7740070111786438 7 0.10559160940504439 0.5621175405808796 8 0.08058768454402697 0.49029600300616427 9 0.05952120675998448 0.4073933402522946 >10 0.30353767740347076 4.066107590093173 >50 0.020587902326538232 1.0730754412448993 >100 0.01759279760658959 2.4598053486437887 >500 0.0020587316324935825 1.2074140006395515 >1k 0.0014972593690862417 2.2285136164108756 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4425 0.6295043346886052 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2368 0.33687373210002647 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2179 0.30998642831332673 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1622 0.23074712561919042 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1387 0.1973158219690611 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1354 0.19262121337138338 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1305 0.18565043090816494 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1017 0.14467930132843196 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 962 0.13685495366563574 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 955 0.13585912759946167 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 905 0.12874608426964693 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 884 0.12575860607112474 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 847 0.12049495400706182 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 760 0.10811825861318416 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 735 0.10456173694827679 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 710 0.10100521528336942 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.845217331925899E-4 0.0 24-25 0.0 0.0 0.0 4.2678259978888485E-4 0.0 26-27 0.0 0.0 0.0 5.690434663851798E-4 0.0 28-29 0.0 0.0 0.0 0.0012803477993666547 0.0 30-31 0.0 0.0 0.0 0.0041255651312925535 0.0 32-33 0.0 0.0 0.0 0.008108869395988812 0.0 34-35 0.0 0.0 0.0 0.014012695359735053 0.0 36-37 0.0 0.0 0.0 0.021267999556146097 0.0 38-39 0.0 0.0 0.0 0.03464052101619782 0.0 40-41 0.0 0.0 0.0 0.054841564072871704 0.0 42-43 0.0 0.0 0.0 0.07575391146252707 0.0 44-45 0.0 0.0 0.0 0.09951147618410833 0.0 46-47 0.0 0.0 0.0 0.12483391043824882 0.0 48-49 0.0 0.0 0.0 0.15470869242347077 0.0 50-51 0.0 0.0 0.0 0.19290573510457598 0.0 52-53 0.0 0.0 0.0 0.23003582128620895 0.0 54-55 0.0 0.0 0.0 0.2722161682320104 0.0 56-57 0.0 0.0 0.0 0.3177396455428248 0.0 58-59 0.0 0.0 0.0 0.3701627748835595 0.0 60-61 0.0 0.0 0.0 0.43304207791912186 0.0 62-63 0.0 0.0 0.0 0.500118076519275 0.0 64-65 0.0 0.0 0.0 0.5637086838878188 0.0 66-67 0.0 0.0 0.0 0.6396759866502403 0.0 68-69 0.0 0.0 0.0 0.731149723871658 0.0 70-71 0.0 0.0 0.0 0.8381298955520717 0.0 72-73 0.0 0.0 0.0 0.9340848500712726 0.0 74-75 0.0 0.0 0.0 1.0359436305542198 0.0 76-77 0.0 0.0 0.0 1.1446309326337891 0.0 78-79 0.0 0.0 0.0 1.284331103631351 0.0 80-81 0.0 0.0 0.0 1.4255250137281736 0.0 82-83 0.0 0.0 0.0 1.5755390975539667 0.0 84-85 0.0 0.0 0.0 1.7239171814139023 0.0 86-87 0.0 0.0 0.0 1.8823957868021748 0.0 88-89 0.0 0.0 0.0 2.070109000275986 0.0 90-91 0.0 0.0 0.0 2.272190561276023 0.0 92-93 0.0 0.0 0.0 2.488640469802286 0.0 94-95 0.0 0.0 0.0 2.6996844653978895 0.0 96-97 0.0 0.0 0.0 2.9128623739924375 0.0 98-99 0.0 0.0 0.0 3.137847934514478 0.0 100-101 0.0 0.0 0.0 3.3912856683557773 0.0 102-103 0.0 0.0 0.0 3.6877573143424565 0.0 104-105 2.845217331925899E-4 0.0 0.0 3.9732748736012202 0.0 106-107 2.845217331925899E-4 0.0 0.0 4.250825824330592 0.0 108-109 2.845217331925899E-4 0.0 0.0 4.546443905117693 0.0 110-111 2.845217331925899E-4 0.0 0.0 4.876987028654184 0.0 112-113 2.845217331925899E-4 0.0 0.0 5.253195890368086 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAGCGG 30 0.00421247 59.583282 3 GTACCTG 850 0.0 34.35535 1 TACCTGG 835 0.0 34.251465 2 GTACACG 125 1.261089E-6 33.37377 1 GTACATG 4010 0.0 32.398834 1 TACATGG 3950 0.0 32.28056 2 ACATGGG 3890 0.0 31.859442 3 TAGGACC 940 0.0 29.791641 4 CATGGGC 945 0.0 28.372992 4 GAGTACT 3090 0.0 28.206833 12-13 GTACTTT 3185 0.0 27.179668 14-15 CATGGGG 1955 0.0 27.124868 4 TGCACCG 90 0.009461752 26.481457 5 AACTCGC 45 0.009534143 26.441898 54-55 AGTACTT 3115 0.0 26.070524 12-13 GTGTAGC 625 0.0 25.745478 1 GTAGGAC 2235 0.0 25.326225 3 TACACGG 165 1.0970649E-5 25.277756 2 GCACCGT 120 0.0012880871 24.789282 6 AGAGTAC 5235 0.0 23.809072 10-11 >>END_MODULE