FastQCFastQC Report
Fri 27 May 2016
SRR939963_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939963_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2199780
Sequences flagged as poor quality0
Sequence length125
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT115060.523052305230523No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT62680.2849375846675577No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT52200.2372964569184191No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT37370.16988062442607899No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC36420.16556201074652918No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC35200.16001600160016002No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT32810.14915127876424006No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA31130.1415141514151415No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG30090.13678640591331861No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC27890.1267854058133086No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC27750.12614897853421705No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27240.1238305648746693No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25450.11569338752057023No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24400.11092018292738365No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA23860.10846539199374483No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG23660.1075562101664712No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT23650.10751075107510752No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC23370.10623789651692442No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA23270.10578330560328761No Hit
CTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACA23180.10537417378101445No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT23000.1045559101364682No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA22460.10210111920282937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC7800.01322.6643108-109
CCGCGCG3500.01122.9152108-109
CGTCGCG1400.01052.7329108-109
CCGTTCG951.1641532E-101034.2639108-109
CCGCGCC9350.0919.4993108-109
CTCCGCC6350.0918.1251110-111
CCGCTCG4350.0734.0896108-109
CCTCGCC9100.0701.82196108-109
CGGCGCC5350.0688.7038108-109
CGTCGCA757.724608E-5655.0338108-109
CCGCGCT1651.8335413E-9595.4853108-109
CCGTGCG1305.291404E-7566.8562108-109
TTGTTCG3850.0510.41592108-109
CATCGCG1052.1176996E-4467.8813108-109
CGTCGCC5300.0463.46732108-109
GGTCGCC4400.0446.61398108-109
CCTCGCA1651.3716417E-6446.61398108-109
CCTCTCG5850.0419.89346108-109
CCTCGCG6200.0396.18982108-109
CGCGTCT2452.730094E-6375.7311110-111
Produced by FastQC (version 0.11.5)