##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939963_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2199780 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.025774850212294 34.0 31.0 34.0 30.0 34.0 2 32.06689305294166 34.0 31.0 34.0 30.0 34.0 3 32.217674949313114 34.0 31.0 34.0 30.0 34.0 4 35.494176690396316 37.0 35.0 37.0 35.0 37.0 5 35.50739664875579 37.0 35.0 37.0 35.0 37.0 6 35.56701715626108 37.0 37.0 37.0 35.0 37.0 7 35.55129240196747 37.0 37.0 37.0 35.0 37.0 8 35.48537353735374 37.0 37.0 37.0 35.0 37.0 9 37.191135931774994 39.0 39.0 39.0 35.0 39.0 10-11 37.17226222622263 39.0 39.0 39.0 35.0 39.0 12-13 37.122199719972 39.0 38.5 39.0 35.0 39.0 14-15 38.63741987835147 41.0 39.0 41.0 35.0 41.0 16-17 38.639451899735434 41.0 39.0 41.0 35.0 41.0 18-19 38.667282637354646 41.0 39.0 41.0 35.0 41.0 20-21 38.71264262789916 41.0 39.0 41.0 35.0 41.0 22-23 38.70385106692487 41.0 39.0 41.0 35.0 41.0 24-25 38.70681477238632 41.0 39.0 41.0 34.5 41.0 26-27 38.66651733355154 41.0 39.0 41.0 34.0 41.0 28-29 38.620495458636775 41.0 39.0 41.0 34.0 41.0 30-31 38.54757907608943 41.0 39.0 41.0 34.0 41.0 32-33 38.43097627944613 40.5 39.0 41.0 34.0 41.0 34-35 38.3305421451236 40.0 38.0 41.0 33.5 41.0 36-37 38.491453918119085 41.0 39.0 41.0 34.0 41.0 38-39 38.46276377637764 41.0 39.0 41.0 34.0 41.0 40-41 38.422016292538345 41.0 39.0 41.0 34.0 41.0 42-43 38.29983498349835 41.0 38.5 41.0 33.0 41.0 44-45 38.1604758203093 40.5 38.0 41.0 33.0 41.0 46-47 38.048977397739776 40.0 38.0 41.0 33.0 41.0 48-49 37.91226849957723 40.0 38.0 41.0 33.0 41.0 50-51 37.76016442553346 40.0 38.0 41.0 33.0 41.0 52-53 37.60903704006764 40.0 37.0 41.0 32.5 41.0 54-55 37.40926342634263 40.0 37.0 41.0 32.0 41.0 56-57 37.176067606760675 40.0 36.0 41.0 32.0 41.0 58-59 36.953145996417824 40.0 36.0 41.0 31.0 41.0 60-61 36.65600287301457 39.0 35.0 41.0 31.0 41.0 62-63 36.38481779996181 39.0 35.0 41.0 31.0 41.0 64-65 36.049239696696944 38.5 35.0 41.0 31.0 41.0 66-67 35.69064611006555 37.5 35.0 40.0 30.0 41.0 68-69 35.300217294456715 37.0 35.0 40.0 30.0 41.0 70-71 34.878479666148436 36.5 35.0 39.0 30.0 41.0 72-73 34.490665884770294 36.0 34.0 39.0 29.5 41.0 74-75 34.10117307185264 35.5 34.0 38.5 29.0 40.0 76-77 33.69832324141505 35.0 34.0 37.0 29.0 39.0 78-79 33.27350985098509 35.0 34.0 37.0 28.5 39.0 80-81 32.8805537371919 35.0 34.0 36.0 28.0 39.0 82-83 32.740860449681335 35.0 34.0 36.0 29.0 37.0 84-85 32.405387811508426 35.0 34.0 36.0 28.5 37.0 86-87 31.84768044986317 35.0 34.0 35.0 26.5 37.0 88-89 16.50294120321123 18.0 16.5 18.5 2.0 19.0 90-91 2.7805955595559553 2.0 2.0 2.0 2.0 2.0 92-93 2.295150651428779 2.0 2.0 2.0 2.0 2.0 94-95 2.101421733082399 2.0 2.0 2.0 2.0 2.0 96-97 2.0000131831364953 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15146546101914282 1101 2 0.14201960828920335 1101 3 0.2493536103460059 1101 4 0.17750609548212992 1101 5 0.1639090217417234 1101 6 0.16391890384521446 1101 7 0.2053223155645938 1101 8 0.2379535625532938 1101 9 0.3013342251493256 1101 10-11 0.2872393937184725 1101 12-13 0.33123770825235255 1101 14-15 0.2769670823290866 1101 16-17 0.3478498687698206 1101 18-19 0.33075663212864015 1101 20-21 0.29363669867500164 1101 22-23 0.280166534523282 1101 24-25 0.26040478832703684 1101 26-27 0.33169570942570203 1101 28-29 0.32561562038098657 1101 30-31 0.2738742192921322 1101 32-33 0.2998218892084026 1101 34-35 0.31074844468503926 1101 36-37 0.257132980876392 1101 38-39 0.29278785275709396 1101 40-41 0.32156261009188114 1101 42-43 0.3766047278251321 1101 44-45 0.29903402879036634 1101 46-47 0.280283390576507 1101 48-49 0.3247624064862009 1101 50-51 0.22564603342380707 1101 52-53 0.20546690489264563 1101 54-55 0.20289717473292512 1101 56-57 0.26564046893988547 1101 58-59 0.23614279949185146 1101 60-61 0.1462312972523918 1101 62-63 0.15357915904576913 1101 64-65 0.2711350159497812 1101 66-67 0.2024462493739847 1101 68-69 0.17574265841098935 1101 70-71 0.1923639233728025 1101 72-73 0.18808857145471336 1101 74-75 0.1845659154125201 1101 76-77 0.15958948625742408 1101 78-79 0.04840135163821202 1101 80-81 0.13777200035023895 1101 82-83 0.18159918352456828 1101 84-85 0.16127372464115552 1101 86-87 0.3152797626839181 1101 88-89 0.9574175471354138 1101 90-91 0.0056095054597902205 1101 92-93 -0.11965948629660383 1101 94-95 0.03528174087651337 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.07288110061158193 1102 2 -0.03264925508827332 1102 3 0.03552594767882056 1102 4 0.07152791173533757 1102 5 0.02197560574865065 1102 6 0.048624788776507444 1102 7 0.044162237477991084 1102 8 0.04632184729435096 1102 9 0.14607962457611023 1102 10-11 0.10769751421576501 1102 12-13 0.09882848009448963 1102 14-15 0.018103140511605886 1102 16-17 0.06408658974608272 1102 18-19 -0.006243224869649566 1102 20-21 0.05867310932111991 1102 22-23 0.08685709690352894 1102 24-25 0.08794921884875606 1102 26-27 0.05139723581828548 1102 28-29 0.08470189972304354 1102 30-31 0.05957892459628766 1102 32-33 0.08279290588481558 1102 34-35 0.026864189502049385 1102 36-37 0.08522350260250988 1102 38-39 0.12621091372786708 1102 40-41 0.12640850520314473 1102 42-43 0.05301607525737495 1102 44-45 0.0509587715379638 1102 46-47 0.06398475698249939 1102 48-49 -0.02205783269374706 1102 50-51 -0.030373653692826963 1102 52-53 0.001360947748231922 1102 54-55 -0.04141658964736905 1102 56-57 -0.07293361224673589 1102 58-59 -0.11266362933904617 1102 60-61 -0.12721481931975376 1102 62-63 -0.15240664050089947 1102 64-65 -0.06354678880850173 1102 66-67 -0.08876676126129013 1102 68-69 -0.08712916658122083 1102 70-71 -0.010560050672118848 1102 72-73 -0.05590050781700029 1102 74-75 -0.04119554129822234 1102 76-77 -0.05267858113199253 1102 78-79 -0.036583578392303195 1102 80-81 -0.09105056959322155 1102 82-83 -0.05816071605644879 1102 84-85 0.051543088360254785 1102 86-87 0.19011031113656784 1102 88-89 1.0718350781588377 1102 90-91 -0.04618927524517025 1102 92-93 -0.11337962921064992 1102 94-95 0.040215847770565194 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.047132484258455065 1103 2 0.2152658388389952 1103 3 0.18582270131187073 1103 4 0.14165509367808937 1103 5 0.12962765739105464 1103 6 0.06908644363931415 1103 7 0.16286824156509283 1103 8 0.11145842042939336 1103 9 0.24260353404262958 1103 10-11 0.1562965506806009 1103 12-13 0.22264992435582798 1103 14-15 0.19348660670029716 1103 16-17 0.21862088937332658 1103 18-19 0.20928286552095443 1103 20-21 0.24723721205347715 1103 22-23 0.22066669091636726 1103 24-25 0.1706319809123329 1103 26-27 0.2010470253618024 1103 28-29 0.21869728556626455 1103 30-31 0.16839631593928317 1103 32-33 0.2548149739960692 1103 34-35 0.24019115232579935 1103 36-37 0.22581140277593903 1103 38-39 0.21567034152290177 1103 40-41 0.24053252609184028 1103 42-43 0.23218909153138867 1103 44-45 0.1902921667489892 1103 46-47 0.23165413155671644 1103 48-49 0.2012495539893706 1103 50-51 0.1411028923507942 1103 52-53 0.1506548700005652 1103 54-55 0.11918380530446626 1103 56-57 0.13369584878127228 1103 58-59 0.14632563135339893 1103 60-61 0.10721054946194641 1103 62-63 0.1415550879695502 1103 64-65 0.1135357386419642 1103 66-67 0.03799830081519673 1103 68-69 -0.017556449690822262 1103 70-71 0.08036660870399004 1103 72-73 0.10302866025152468 1103 74-75 0.1167162961125996 1103 76-77 0.09112667685617737 1103 78-79 0.09417068987205113 1103 80-81 0.1221221399734631 1103 82-83 0.11936657936165318 1103 84-85 0.12261981419490553 1103 86-87 0.24282497266787928 1103 88-89 0.7732077575204528 1103 90-91 -0.5347811488555019 1103 92-93 -0.31899170666388255 1103 94-95 -0.10829088015854538 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.019229815111117432 1104 2 0.027308110123804852 1104 3 0.12039730932514914 1104 4 0.13631641615481271 1104 5 0.1297619971291084 1104 6 0.0886431857945027 1104 7 0.17198057949609336 1104 8 0.08900245114623573 1104 9 0.15565785517319597 1104 10-11 0.12788691166148425 1104 12-13 0.1746475593231196 1104 14-15 0.19649868533314674 1104 16-17 0.17110953812483132 1104 18-19 0.20901944326104172 1104 20-21 0.1814591272134436 1104 22-23 0.013478728900210513 1104 24-25 0.20400045082457297 1104 26-27 0.2045291909959701 1104 28-29 0.17227769989411001 1104 30-31 0.2075370561083716 1104 32-33 0.1871820616177331 1104 34-35 0.24531127257007057 1104 36-37 0.1669234356863214 1104 38-39 0.1317350058945621 1104 40-41 0.1791364075098656 1104 42-43 0.0850699286108707 1104 44-45 0.09504304666916852 1104 46-47 0.14214231869401317 1104 48-49 0.1375996719296566 1104 50-51 0.1272879490149066 1104 52-53 0.07731425414678483 1104 54-55 0.11322942099543098 1104 56-57 0.18192253871851705 1104 58-59 0.09004396747203458 1104 60-61 0.04839597923528771 1104 62-63 -0.006266653406235889 1104 64-65 0.01077283164028131 1104 66-67 0.07497485434554818 1104 68-69 -2.0855499153071833E-4 1104 70-71 -0.02658762666759884 1104 72-73 -0.029673225272027537 1104 74-75 -0.04228779930986093 1104 76-77 -0.09549398355429162 1104 78-79 0.012778775021395461 1104 80-81 0.020643473909899512 1104 82-83 0.06362161090035556 1104 84-85 0.1067574658770738 1104 86-87 0.12241093677855375 1104 88-89 1.115192376028773 1104 90-91 -0.575539988711407 1104 92-93 -0.31899170666388255 1104 94-95 -0.10829088015854538 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.08207345169783054 1105 2 -0.11412476529046955 1105 3 -0.0371081470200636 1105 4 -0.0348682443159376 1105 5 0.01360028621459719 1105 6 -0.007261033868473987 1105 7 0.021429227314136767 1105 8 -0.09519256632984963 1105 9 -0.001025985319571987 1105 10-11 -0.07408943330639772 1105 12-13 0.07846207247180104 1105 14-15 -0.044619957008457334 1105 16-17 0.06449855312982322 1105 18-19 0.021799872901510753 1105 20-21 0.04759738678746572 1105 22-23 -0.14431960863053916 1105 24-25 -0.008268724703725638 1105 26-27 -0.019749602113179776 1105 28-29 -0.10266209344224819 1105 30-31 -0.07314079632656245 1105 32-33 -0.07873142244146436 1105 34-35 0.011021660575849523 1105 36-37 -0.18944894122540745 1105 38-39 -0.12163327663646584 1105 40-41 -0.04657850102096717 1105 42-43 -0.07642538246111741 1105 44-45 -0.10719863346527347 1105 46-47 -0.07767193477760514 1105 48-49 -0.053760437325294674 1105 50-51 -0.18403656184969464 1105 52-53 -0.1901400202062007 1105 54-55 -0.14814284126529742 1105 56-57 -0.17801339966415952 1105 58-59 -0.20097734063926964 1105 60-61 -0.3051334996208368 1105 62-63 -0.2762078121317586 1105 64-65 -0.20392009576301007 1105 66-67 -0.185041101499813 1105 68-69 -0.13000346073501134 1105 70-71 -0.12966259621064324 1105 72-73 -0.17313110532445108 1105 74-75 -0.23196000763995528 1105 76-77 -0.07024187792171688 1105 78-79 -0.11719867893432223 1105 80-81 -0.19330079736687367 1105 82-83 -0.2258916643451201 1105 84-85 -0.2505249719426246 1105 86-87 -0.06752601112849987 1105 88-89 0.9790499741290795 1105 90-91 -0.7892152023866208 1105 92-93 -0.31899170666388255 1105 94-95 -0.10829088015854538 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.06199446586578006 1106 2 -0.0366249288404461 1106 3 -0.05237500202393619 1106 4 -0.02993174884491623 1106 5 -0.059375762545478494 1106 6 -0.037263956342357574 1106 7 -0.11096817161745065 1106 8 -0.03146844710244068 1106 9 -0.011556223401683496 1106 10-11 -0.14789751580833865 1106 12-13 -0.14747572597120495 1106 14-15 -0.07060593008608151 1106 16-17 -0.14557884910038155 1106 18-19 -0.132936120292527 1106 20-21 -0.05376308241576311 1106 22-23 -0.08066371855060339 1106 24-25 -0.23602523701634937 1106 26-27 -0.2962721000674975 1106 28-29 -0.1260715472691203 1106 30-31 -0.11787992910886658 1106 32-33 -0.06436916309203866 1106 34-35 -0.19602937663574949 1106 36-37 -0.10872371823798943 1106 38-39 -0.1333308378229603 1106 40-41 -0.054316867141906755 1106 42-43 -0.32796929903509664 1106 44-45 -0.08944048612897859 1106 46-47 -0.21186462207946732 1106 48-49 -0.16495271239551101 1106 50-51 -0.23052213226583973 1106 52-53 -0.251384655076464 1106 54-55 -0.20123725926400482 1106 56-57 -0.38789206605238036 1106 58-59 -0.28301981944942867 1106 60-61 -0.27763234429615835 1106 62-63 -0.18813602878373104 1106 64-65 -0.2344834685473529 1106 66-67 -0.245579638493588 1106 68-69 -0.27641806902876453 1106 70-71 -0.227029113001727 1106 72-73 -0.22727705208933457 1106 74-75 -0.3218390301719509 1106 76-77 -0.25203936981253605 1106 78-79 -0.22930792898791452 1106 80-81 -0.11996741451622484 1106 82-83 -0.1837470484533128 1106 84-85 -0.23393567132043103 1106 86-87 -0.13113695472189946 1106 88-89 0.7655700657323052 1106 90-91 -0.3040952509745347 1106 92-93 -0.28832058610512146 1106 94-95 -0.10829088015854538 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.014295501638230235 1107 2 -0.10837258490145985 1107 3 -0.002271168613042107 1107 4 -0.01653083015472845 1107 5 -0.04858982342698681 1107 6 -0.09672219856047803 1107 7 -0.08096508036950212 1107 8 -0.045519590677962185 1107 9 -0.021551409848079572 1107 10-11 -0.034191778086970714 1107 12-13 -0.06380428266181326 1107 14-15 -0.0795869909918352 1107 16-17 -0.13330533206595163 1107 18-19 -0.08466836825839152 1107 20-21 -0.06200339746688144 1107 22-23 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2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores warn #Quality Count 2 17565.0 3 3360.0 4 2393.0 5 2133.0 6 2315.0 7 2830.0 8 3080.0 9 3804.0 10 4640.0 11 5727.0 12 7036.0 13 7975.0 14 8655.0 15 9131.0 16 10076.0 17 11494.0 18 14488.0 19 17955.0 20 20874.0 21 28729.0 22 34674.0 23 46635.0 24 72324.0 25 127870.0 26 270553.0 27 739631.0 28 699226.0 29 23806.0 30 801.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.36101791997382 19.16428006437007 14.491630981279947 22.983071034376167 2 14.785488449902603 21.108027448386533 39.29074242021307 24.815741681497798 3 19.252901399047595 25.78139602783815 29.111768726695235 25.853933846419018 4 12.566515851921386 17.06780841979257 38.354563828838906 32.01111189944714 5 13.562701570080554 38.73065484379925 33.92071686746166 13.785926718658539 6 30.908409022720452 36.7966796679668 18.261326132613263 14.033585176699487 7 28.188364290974555 32.771731718626405 21.625253434434352 17.414650555964688 8 26.68966896689669 33.37606487921519 20.72807280728073 19.20619334660739 9 26.551291492785644 17.47679313385884 20.723072307230723 35.2488430661248 10-11 24.87804390192234 26.697054733524052 27.506004044973853 20.918897319579756 12-13 26.19370221741863 24.099411635680084 27.27366953177474 22.433216615126543 14-15 22.87407053163068 25.715265033441998 25.705195840127576 25.70546859479975 16-17 22.459706106306328 28.67227330067848 26.374674682638 22.493345910377197 18-19 22.653050786777367 28.097925226315702 27.284632126884546 21.96439186002239 20-21 23.872404717565036 26.793640965633834 26.637372248903446 22.696582067897676 22-23 23.231761189198945 26.810254633844092 26.787843164263116 23.170141012693847 24-25 23.373178226913602 27.011632981479966 26.750811444780844 22.86437734682559 26-27 22.719294656738402 26.964310067370374 27.20894816754403 23.1074471083472 28-29 22.590852328286246 28.152640912539646 26.528229786007596 22.72827697316651 30-31 22.803712189400756 27.054409986453194 26.43832565074689 23.70355217339916 32-33 22.73781111489942 27.282213887941815 26.357176951926352 23.62279804523241 34-35 23.7588065704812 27.140693288140895 26.15445740433807 22.94604273703983 36-37 23.665160757130945 26.33017968176789 26.883621892754633 23.121037668346535 38-39 22.82360054187237 27.312731273127312 26.462532616898056 23.401135568102266 40-41 23.249393121130296 27.20549327660039 26.524175144787204 23.020938457482114 42-43 22.996140523143225 27.052341597796143 25.896885143059762 24.054632736000872 44-45 23.05182436757197 26.83986915031349 26.443851555794772 23.66445492631977 46-47 23.885638563856386 27.375964869214194 25.903226686304993 22.835169880624427 48-49 23.07415006667726 27.25730421127065 26.15250965382604 23.516036068226054 50-51 22.643603127224658 27.357828835455216 26.974835642505745 23.023732394814385 52-53 23.480598059805978 27.338802062024385 26.051900644609916 23.12869923355972 54-55 22.75988697804 27.05892193633866 26.51999693601476 23.66119414960658 56-57 23.1744083499259 26.997404285883135 26.347293820291117 23.480893543899846 58-59 23.570129740246752 26.56556564747384 26.321154842757004 23.543149769522405 60-61 22.58387202356599 27.252861649801346 27.13430433952486 23.028961987107802 62-63 22.550260151074234 27.186679392184537 27.14817553304774 23.114884923693488 64-65 22.361955987841505 27.632782118745137 26.528534577458597 23.476727315954765 66-67 22.729204738655685 27.15642018747329 26.187323277782326 23.9270517960887 68-69 23.101890744842184 26.70406606514869 26.761753966781953 23.432289223227176 70-71 23.252370691614615 26.813454072679992 26.27076343998036 23.66341179572503 72-73 22.996163252688905 26.541836001782 26.974788387929706 23.487212357599397 74-75 22.69436034512542 27.9163143587086 26.269967905881497 23.119357390284485 76-77 23.10324214239606 26.801021011192027 26.41911918464574 23.676617661766176 78-79 23.309477056811154 26.983519417139195 26.48626299879919 23.220740527250456 80-81 23.727577303184862 27.00838265644746 26.182300048186637 23.081739992181035 82-83 23.417216574141587 26.88827074177048 26.807898483655517 22.88661420043241 84-85 23.03067193780104 27.57176344265414 26.558207311232785 22.83935730831204 86-87 23.26681362282697 27.54941586517371 26.2314473151255 22.952323196873824 88-89 32.42950062632621 17.616287241902757 31.96060806739704 17.993604064373997 90-91 31.073826061793376 13.655628767630299 15.226303587371119 40.044241583205206 92-93 23.312619825162397 16.57627917943881 32.714645363230474 27.39645563216832 94-95 15.185003132855297 6.516586019314197 26.172474181731953 52.125936666098546 96-97 26.299037241401173 6.863474613659319 14.347331037318895 52.49015710762062 98-99 57.719473303202825 14.137204646097057 18.734388553018594 9.408933497681517 100-101 31.493115146677308 6.739291206404734 11.754469578691584 50.013124068226375 102-103 23.658150416607317 1.934397065193062 35.917374254743336 38.49007826345628 104-105 43.43998886003429 4.5351957812805175 37.53851968663421 14.486295672050984 106-107 5.091532187019974 1.5049395368920497 6.116662902973649 87.28686537311432 108-109 18.759131300890182 9.500999924583061 19.034585167828695 52.70528360669806 110-111 40.54949251571852 2.5019225437215216 23.002815246724577 33.94576969383538 112-113 27.879403232294326 2.7324926076819462 37.25827795115882 32.12982620886491 114-115 27.58671149651467 3.669884292853909 22.250685617779062 46.49271859285236 116-117 18.80938704844168 3.935903306302376 19.2602341478746 57.994475497381345 118-119 19.477771699151507 4.078418038856118 14.689121237440006 61.75468902455237 120-121 25.001913731312126 4.1083751841241485 28.5999605655366 42.28975051902713 122-123 34.08306272753705 1.5253980516382668 32.78876563295409 31.602773587870587 124-125 38.46828411417319 1.6443777168631255 17.893921311106144 41.99341685785753 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 16.0 1 40.0 2 93.5 3 146.5 4 185.0 5 203.0 6 198.5 7 194.5 8 189.5 9 179.5 10 165.0 11 147.0 12 150.0 13 160.0 14 159.5 15 175.5 16 215.5 17 290.5 18 399.5 19 559.0 20 880.0 21 1404.5 22 2272.5 23 3610.5 24 5449.5 25 7989.5 26 11121.0 27 14603.0 28 18166.5 29 22227.5 30 26765.5 31 31742.0 32 37058.5 33 42260.0 34 47390.0 35 52592.5 36 57650.0 37 62220.0 38 66022.5 39 68863.0 40 71558.5 41 74538.5 42 78319.0 43 84121.5 44 90094.5 45 91825.0 46 89331.5 47 87279.0 48 87107.5 49 87184.0 50 87657.0 51 86784.0 52 83087.5 53 76648.0 54 68984.5 55 62513.5 56 56510.5 57 49924.0 58 43161.0 59 36441.0 60 29738.5 61 23725.0 62 18567.0 63 14169.0 64 10417.5 65 7449.0 66 5358.0 67 3868.5 68 2832.0 69 2040.0 70 1389.0 71 896.5 72 582.0 73 384.5 74 307.5 75 239.0 76 171.0 77 119.0 78 69.0 79 42.0 80 34.0 81 30.0 82 21.5 83 13.5 84 7.5 85 11.0 86 14.0 87 9.0 88 6.0 89 5.0 90 6.5 91 8.5 92 4.5 93 2.0 94 3.0 95 3.5 96 4.0 97 5.5 98 6.0 99 3.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 6.818863704552274E-4 3 0.042140577694133055 4 0.04800480048004801 5 0.05014137777414105 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.909681877278637E-4 12-13 0.0017047159261380682 14-15 4.5459091363681824E-5 16-17 2.2729545681840913E-4 18-19 0.00647792051932466 20-21 0.0019774704743201594 22-23 6.136977334097047E-4 24-25 0.0 26-27 0.0 28-29 0.001318313649546773 30-31 0.0 32-33 0.0013637727409104548 34-35 9.091818272736365E-5 36-37 9.091818272736365E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 1.818363654547273E-4 46-47 0.0 48-49 2.0456591113656822E-4 50-51 9.091818272736365E-5 52-53 0.0 54-55 0.0013865022865922956 56-57 0.0 58-59 0.0 60-61 0.0 62-63 2.2729545681840912E-5 64-65 6.818863704552273E-5 66-67 0.0 68-69 5.455090963641818E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 4.318613679549773E-4 80-81 0.0 82-83 7.273454618189092E-4 84-85 2.2729545681840912E-5 86-87 0.0014092318322741365 88-89 19.308589949904082 90-91 26.244647191991927 92-93 40.33087399649056 94-95 44.78779696151433 96-97 48.377064979225196 98-99 93.02962114393257 100-101 51.59345480002546 102-103 53.084990317213546 104-105 74.86275900317304 106-107 81.68121357590304 108-109 12.598305285073963 110-111 39.1725081599069 112-113 52.02617989071634 114-115 86.79724790660885 116-117 66.44364436443644 118-119 71.78938348380292 120-121 80.40281300857359 122-123 75.84442535162607 124-125 49.94981316313449 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2199780.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.8276250103073 #Duplication Level Percentage of deduplicated Percentage of total 1 66.02485813188898 26.29613291035613 2 15.18401941055105 12.09486862465309 3 6.641001489378646 7.934859510355952 4 3.6279129875373206 5.7796463215064025 5 2.171238500920867 4.323763641130902 6 1.4348714997447305 3.4288514417886224 7 0.9702631819874786 2.705029472145339 8 0.671762239401821 2.1403755653584007 9 0.5206758033511035 1.8663552583027432 >10 2.5267349022096655 17.64495658423158 >50 0.14727580284644867 4.001394416626741 >100 0.06842376003975245 4.969500834514511 >500 0.0046818114148865385 1.2677375230622019 >1k 0.005937907160343893 4.500775192638727 >5k 2.2838104462861124E-4 0.5224670373072128 >10k+ 1.1419052231430562E-4 0.5232856660216566 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11506 0.523052305230523 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6268 0.2849375846675577 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5220 0.2372964569184191 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3737 0.16988062442607899 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3642 0.16556201074652918 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3520 0.16001600160016002 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3281 0.14915127876424006 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3113 0.1415141514151415 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3009 0.13678640591331861 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2789 0.1267854058133086 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2775 0.12614897853421705 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2724 0.1238305648746693 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2545 0.11569338752057023 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2440 0.11092018292738365 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2386 0.10846539199374483 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2366 0.1075562101664712 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 2365 0.10751075107510752 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2337 0.10623789651692442 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2327 0.10578330560328761 No Hit CTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACA 2318 0.10537417378101445 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 2300 0.1045559101364682 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2246 0.10210111920282937 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 4.5459091363681824E-5 14-15 0.0 0.0 0.0 0.0 4.5459091363681824E-5 16-17 0.0 0.0 0.0 0.0 4.5459091363681824E-5 18-19 0.0 0.0 0.0 0.0 4.5459091363681824E-5 20-21 0.0 0.0 0.0 4.5459091363681824E-5 4.5459091363681824E-5 22-23 0.0 0.0 0.0 1.3637727409104546E-4 4.5459091363681824E-5 24-25 0.0 0.0 0.0 3.636727309094546E-4 4.5459091363681824E-5 26-27 0.0 0.0 0.0 7.955340988644319E-4 4.5459091363681824E-5 28-29 0.0 0.0 0.0 0.0017047159261380685 4.5459091363681824E-5 30-31 0.0 0.0 0.0 0.005455090963641819 4.5459091363681824E-5 32-33 0.0 0.0 0.0 0.012296684213875933 4.5459091363681824E-5 34-35 0.0 0.0 0.0 0.02368418660047823 4.5459091363681824E-5 36-37 0.0 0.0 0.0 0.0403904026766313 4.5459091363681824E-5 38-39 0.0 0.0 0.0 0.06830228477393194 4.5459091363681824E-5 40-41 0.0 0.0 0.0 0.10453318059078635 4.5459091363681824E-5 42-43 0.0 0.0 0.0 0.14058224004218606 4.5459091363681824E-5 44-45 0.0 0.0 0.0 0.1851094200329124 4.5459091363681824E-5 46-47 0.0 0.0 0.0 0.23688732509614596 4.5459091363681824E-5 48-49 0.0 0.0 0.0 0.29000627335460816 4.5459091363681824E-5 50-51 0.0 0.0 0.0 0.35235341715989776 4.5459091363681824E-5 52-53 0.0 0.0 0.0 0.41549609506405183 4.5459091363681824E-5 54-55 0.0 0.0 0.0 0.485048504850485 4.5459091363681824E-5 56-57 0.0 0.0 0.0 0.5657611215667021 4.5459091363681824E-5 58-59 0.0 0.0 0.0 0.6532926019874714 4.5459091363681824E-5 60-61 0.0 0.0 0.0 0.7441880551691533 4.5459091363681824E-5 62-63 0.0 0.0 0.0 0.8420842084208421 4.5459091363681824E-5 64-65 0.0 0.0 0.0 0.943526170798898 4.5459091363681824E-5 66-67 0.0 0.0 0.0 1.060583331060379 4.5459091363681824E-5 68-69 0.0 0.0 0.0 1.1962105301439234 4.5459091363681824E-5 70-71 0.0 0.0 0.0 1.3457027520933913 4.5459091363681824E-5 72-73 0.0 0.0 0.0 1.499990908181727 4.5459091363681824E-5 74-75 0.0 0.0 0.0 1.6574612006655212 4.5459091363681824E-5 76-77 0.0 0.0 0.0 1.8292965660202385 4.5459091363681824E-5 78-79 0.0 0.0 0.0 1.8771195301348316 4.5459091363681824E-5 80-81 0.0 4.5459091363681824E-5 0.0 1.8777332278682413 4.5459091363681824E-5 82-83 0.0 4.5459091363681824E-5 0.0 1.8777332278682413 4.5459091363681824E-5 84-85 4.5459091363681824E-5 4.5459091363681824E-5 0.0 1.8777332278682413 4.5459091363681824E-5 86-87 4.5459091363681824E-5 4.5459091363681824E-5 0.0 1.8777332278682413 4.5459091363681824E-5 88-89 4.5459091363681824E-5 4.5459091363681824E-5 0.0 1.8777332278682413 4.5459091363681824E-5 90-91 4.5459091363681824E-5 4.5459091363681824E-5 0.0 1.8777332278682413 4.5459091363681824E-5 92-93 4.5459091363681824E-5 4.5459091363681824E-5 0.0 1.8777332278682413 4.5459091363681824E-5 94-95 4.5459091363681824E-5 4.5459091363681824E-5 0.0 1.8777332278682413 4.5459091363681824E-5 96-97 4.5459091363681824E-5 4.5459091363681824E-5 0.0 1.8777332278682413 4.5459091363681824E-5 98-99 4.5459091363681824E-5 4.5459091363681824E-5 0.0 1.8777332278682413 4.5459091363681824E-5 100-101 4.5459091363681824E-5 4.5459091363681824E-5 0.0 1.8777332278682413 4.5459091363681824E-5 102-103 4.5459091363681824E-5 4.5459091363681824E-5 0.0 1.8777332278682413 4.5459091363681824E-5 104-105 4.5459091363681824E-5 4.5459091363681824E-5 0.0 1.8777332278682413 4.5459091363681824E-5 106-107 4.5459091363681824E-5 4.5459091363681824E-5 0.0 1.8777332278682413 4.5459091363681824E-5 108-109 4.5459091363681824E-5 4.5459091363681824E-5 0.0 1.8777332278682413 4.5459091363681824E-5 110-111 4.5459091363681824E-5 4.5459091363681824E-5 0.0 1.8777332278682413 4.5459091363681824E-5 112-113 4.5459091363681824E-5 4.5459091363681824E-5 0.0 1.8777332278682413 4.5459091363681824E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCTCC 780 0.0 1322.6643 108-109 CCGCGCG 350 0.0 1122.9152 108-109 CGTCGCG 140 0.0 1052.7329 108-109 CCGTTCG 95 1.1641532E-10 1034.2639 108-109 CCGCGCC 935 0.0 919.4993 108-109 CTCCGCC 635 0.0 918.1251 110-111 CCGCTCG 435 0.0 734.0896 108-109 CCTCGCC 910 0.0 701.82196 108-109 CGGCGCC 535 0.0 688.7038 108-109 CGTCGCA 75 7.724608E-5 655.0338 108-109 CCGCGCT 165 1.8335413E-9 595.4853 108-109 CCGTGCG 130 5.291404E-7 566.8562 108-109 TTGTTCG 385 0.0 510.41592 108-109 CATCGCG 105 2.1176996E-4 467.8813 108-109 CGTCGCC 530 0.0 463.46732 108-109 GGTCGCC 440 0.0 446.61398 108-109 CCTCGCA 165 1.3716417E-6 446.61398 108-109 CCTCTCG 585 0.0 419.89346 108-109 CCTCGCG 620 0.0 396.18982 108-109 CGCGTCT 245 2.730094E-6 375.7311 110-111 >>END_MODULE