FastQCFastQC Report
Fri 27 May 2016
SRR939963_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939963_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2199780
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT117550.5343716189800798No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT63170.2871650801443781No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57290.2604351344225332No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT53120.24147869332387784No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT39000.17729045631835913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38760.17619943812563074No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC36760.16710761985289438No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC34770.1580612606715217No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT33500.15228795606833412No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC29780.13537717408104447No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC29500.13410431952286136No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG29080.13219503768558674No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27550.12523979670694343No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA25490.11587522388602496No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25300.115011501150115No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG25220.11464782841920555No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA23940.10882906472465428No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA23490.1067834056132886No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT22990.10451045104510452No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA22930.10423769649692241No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC22840.10382856467464928No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA22530.10241933284237514No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG600.001301056939.71445
TAAGGTG17450.029.7004555
AGGTAAG17550.027.8340232
GTACATG123850.027.564321
GGTAAGG17800.027.1084233
TACATGG123800.026.8986472
TAAGGTA11800.026.7567374
GTAAGGT18750.026.3703634
ACATGGG120350.026.3332713
GAGTACT79600.025.07642212-13
AGAGTAC147000.024.18280810-11
GTACTTT81350.024.13500614-15
GTGTAAG13900.023.1455551
AAGGTAA20100.022.8235591
CATGGGG50250.022.5245864
AGTACTT84800.022.31091512-13
CATGGGA67950.022.005114
TGTAAGG15550.020.6872432
GTGTGCA29750.020.5985379
ACTTTTT94650.020.58763116-17
Produced by FastQC (version 0.11.5)