FastQCFastQC Report
Fri 27 May 2016
SRR939962_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939962_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2127021
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT55240.2597059455454366No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA49590.2331429732005467No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC40750.19158249965562166No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT31610.14861160280034846No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA31290.14710715126931045No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA27000.12693809793133212No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24240.11396220347612929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGCG550.02664.2107108-109
CCGCTCC3950.02349.4514108-109
CCGCTCG1100.02220.1755108-109
CCGCGCA352.8539944E-92093.3083108-109
CCGCGCC3600.01831.6449108-109
CGGCGCA150.00308498181628.1287108-109
CCGCGTG200.00342919931543.9628110-111
CGCCGTC609.616997E-91543.9626110-111
CGCCGCT458.398894E-61372.4114110-111
CTCCGCC2950.01360.7806110-111
CCTCGCG1550.01260.4868108-109
CGTCGCC2150.01249.494108-109
TTGTTCG2550.01245.0397108-109
CGGCGTT501.1519727E-51235.1702110-111
CGTCGCG602.462366E-81221.0966108-109
CCGCGCG1100.01110.0878108-109
CTCGTCG300.0077140391029.3085110-111
CGCCGCA300.0077140391029.3085110-111
CCGTTCG502.3303768E-5976.87726108-109
CATCGCG756.008668E-8976.8772108-109
Produced by FastQC (version 0.11.5)