##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939962_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2127021 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.039275587782164 34.0 31.0 34.0 30.0 34.0 2 32.15568769654836 34.0 31.0 34.0 30.0 34.0 3 32.254790620308874 34.0 31.0 34.0 30.0 34.0 4 35.50046661504518 37.0 35.0 37.0 33.0 37.0 5 35.530855595689935 37.0 35.0 37.0 35.0 37.0 6 35.66467938022239 37.0 37.0 37.0 35.0 37.0 7 35.646255490660415 37.0 37.0 37.0 35.0 37.0 8 35.6830139429747 37.0 37.0 37.0 35.0 37.0 9 37.37466437801977 39.0 39.0 39.0 35.0 39.0 10-11 37.34377446202929 39.0 39.0 39.0 35.0 39.0 12-13 37.29566680347773 39.0 39.0 39.0 35.0 39.0 14-15 38.85309571461683 41.0 40.0 41.0 36.0 41.0 16-17 38.86209962195954 41.0 40.0 41.0 36.0 41.0 18-19 38.88349785921248 41.0 40.0 41.0 36.0 41.0 20-21 38.937554448216545 41.0 40.0 41.0 36.0 41.0 22-23 38.93979161465731 41.0 40.0 41.0 36.0 41.0 24-25 38.96957928483076 41.0 40.0 41.0 36.0 41.0 26-27 38.937192439566886 41.0 39.0 41.0 35.0 41.0 28-29 38.89147450824416 41.0 39.0 41.0 35.0 41.0 30-31 38.81481047906908 41.0 39.0 41.0 35.0 41.0 32-33 38.728710482877226 41.0 39.0 41.0 35.0 41.0 34-35 38.684323756088915 41.0 39.0 41.0 34.0 41.0 36-37 38.852485706535106 41.0 39.0 41.0 35.0 41.0 38-39 38.80971932105983 41.0 39.0 41.0 35.0 41.0 40-41 38.73253578596544 41.0 39.0 41.0 35.0 41.0 42-43 38.69910546252247 41.0 39.0 41.0 34.5 41.0 44-45 38.63771020596411 41.0 39.0 41.0 34.0 41.0 46-47 38.54913162587488 41.0 39.0 41.0 34.0 41.0 48-49 38.48625213385293 41.0 39.0 41.0 34.0 41.0 50-51 38.39151846643733 41.0 39.0 41.0 33.0 41.0 52-53 38.32307532459717 41.0 39.0 41.0 33.0 41.0 54-55 38.20704661590083 40.5 38.5 41.0 33.0 41.0 56-57 38.0330255319529 40.0 38.0 41.0 33.0 41.0 58-59 37.898870768083626 40.0 38.0 41.0 33.0 41.0 60-61 37.714847667230366 40.0 38.0 41.0 32.5 41.0 62-63 37.50551898641339 40.0 37.0 41.0 32.0 41.0 64-65 37.249830631667486 40.0 37.0 41.0 32.0 41.0 66-67 36.961251440394804 39.5 36.0 41.0 31.0 41.0 68-69 36.56960345008348 39.0 36.0 41.0 31.0 41.0 70-71 36.154612953985875 39.0 35.0 40.5 30.5 41.0 72-73 35.77211437968877 37.5 35.0 40.0 30.0 41.0 74-75 35.353243573993865 37.0 35.0 39.5 30.0 41.0 76-77 34.868088749476385 37.0 35.0 39.0 30.0 41.0 78-79 34.37746359814971 36.0 34.5 39.0 29.0 40.0 80-81 33.89969657093184 36.0 34.0 37.5 29.0 39.0 82-83 33.64284156103771 35.5 34.0 37.0 29.0 39.0 84-85 33.17387275442979 35.0 34.0 37.0 29.0 39.0 86-87 32.52093655868936 35.0 34.0 36.5 28.5 37.5 88-89 16.743697405902434 18.0 17.0 18.5 2.0 19.5 90-91 2.8486281987813 2.0 2.0 2.0 2.0 2.0 92-93 2.328879921730909 2.0 2.0 2.0 2.0 2.0 94-95 2.1107273976138456 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.21631725872044427 1101 2 0.19699484037958115 1101 3 0.24300847432377992 1101 4 0.2319557066277227 1101 5 0.20401788937665088 1101 6 0.20826524516922262 1101 7 0.2747363181416844 1101 8 0.29261553834553666 1101 9 0.35515303460059755 1101 10-11 0.3058036856852837 1101 12-13 0.38541105162531863 1101 14-15 0.4266173869685659 1101 16-17 0.41836126777402427 1101 18-19 0.462928220885793 1101 20-21 0.4019772542834872 1101 22-23 0.3342231743128323 1101 24-25 0.43493258033026905 1101 26-27 0.4186028111829927 1101 28-29 0.40522693430522594 1101 30-31 0.3745500080613482 1101 32-33 0.47512322897897263 1101 34-35 0.39669396348258346 1101 36-37 0.42821660832996145 1101 38-39 0.36277596203538565 1101 40-41 0.46334005886505025 1101 42-43 0.43813968441449447 1101 44-45 0.404579161070572 1101 46-47 0.40319796401827546 1101 48-49 0.45258427501073584 1101 50-51 0.41250383344629427 1101 52-53 0.3381363936952084 1101 54-55 0.3643317978999363 1101 56-57 0.3914467423551571 1101 58-59 0.4065851360586237 1101 60-61 0.32791721292070264 1101 62-63 0.389558716749022 1101 64-65 0.37525733329778177 1101 66-67 0.2691650530895373 1101 68-69 0.30872287768396234 1101 70-71 0.3043494248357206 1101 72-73 0.21949089226644247 1101 74-75 0.19303147570889934 1101 76-77 0.25689618578556406 1101 78-79 0.218038175314355 1101 80-81 0.2611405893397887 1101 82-83 0.26452723743301476 1101 84-85 0.3487895282497391 1101 86-87 0.39303492140187046 1101 88-89 0.7064251307205112 1101 90-91 -0.13608793204132397 1101 92-93 -0.17200651119221977 1101 94-95 0.013606544193877745 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.07053331497649395 1102 2 0.016381557457101792 1102 3 -0.006071832727954529 1102 4 0.08168676810645081 1102 5 0.13969461191394572 1102 6 0.07956924721427328 1102 7 0.12048353381533161 1102 8 0.14266429758848886 1102 9 0.1702634556132523 1102 10-11 0.17543030665396486 1102 12-13 0.1449133181148312 1102 14-15 0.1945917691673884 1102 16-17 0.2376059676390483 1102 18-19 0.09806548841150686 1102 20-21 0.14330920741991093 1102 22-23 0.14837945005345432 1102 24-25 0.15928295678108384 1102 26-27 0.17647240571858447 1102 28-29 0.20731963682965926 1102 30-31 0.16710125786541852 1102 32-33 0.2726740106522314 1102 34-35 0.21762972015555704 1102 36-37 0.27219597270300255 1102 38-39 0.21402713367738357 1102 40-41 0.24733713857037287 1102 42-43 0.2237226229925824 1102 44-45 0.14156117958751935 1102 46-47 0.22142013995340903 1102 48-49 0.1962674019983197 1102 50-51 0.15266808950228494 1102 52-53 0.20357929278198128 1102 54-55 0.19376912570406546 1102 56-57 0.15049034584730236 1102 58-59 0.1571050768532274 1102 60-61 0.12798485519301295 1102 62-63 0.10782444462931551 1102 64-65 0.051007315677125575 1102 66-67 0.1280388671143129 1102 68-69 0.03774141250816143 1102 70-71 0.1121207119009 1102 72-73 -0.002242159557219736 1102 74-75 0.06987309706155287 1102 76-77 -0.006052891049947107 1102 78-79 0.06825893318132614 1102 80-81 0.06195934486925836 1102 82-83 0.17342740354055053 1102 84-85 0.11006332796759466 1102 86-87 0.06149205692521775 1102 88-89 0.7806059806987875 1102 90-91 0.00713169284387849 1102 92-93 -0.09394596136469158 1102 94-95 0.0634098766540685 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.0067158902141741805 1103 2 0.10129501383318029 1103 3 0.10165678338504591 1103 4 0.07402251692104045 1103 5 0.07759734182864975 1103 6 0.03488335729985437 1103 7 0.06562860127532133 1103 8 0.06985185821933015 1103 9 0.07208487014288778 1103 10-11 0.13045030905610133 1103 12-13 0.14554208235954746 1103 14-15 0.20667687458934125 1103 16-17 0.23258560594306488 1103 18-19 0.1433303547079987 1103 20-21 0.15239010294444455 1103 22-23 0.16776591986815248 1103 24-25 0.1475966675372149 1103 26-27 0.16711588048025305 1103 28-29 0.17146313674189173 1103 30-31 0.1392215946161457 1103 32-33 0.12899496477245265 1103 34-35 0.18941899328355305 1103 36-37 0.10836516109924332 1103 38-39 0.06769855226190913 1103 40-41 0.08506104280110804 1103 42-43 0.22345058621731084 1103 44-45 0.19188233696541346 1103 46-47 0.17562351875280768 1103 48-49 0.1535976638824721 1103 50-51 0.06812881201142318 1103 52-53 0.14813871125974742 1103 54-55 0.18090383934173104 1103 56-57 0.12079573535776689 1103 58-59 0.11489677470146376 1103 60-61 0.03711430671555149 1103 62-63 0.033134438233886954 1103 64-65 0.0894112091906365 1103 66-67 0.013821403995827097 1103 68-69 -0.017727912522403244 1103 70-71 0.008123105824523691 1103 72-73 0.06436476167427685 1103 74-75 0.10108444089242852 1103 76-77 0.036782635515720585 1103 78-79 0.0479809013314636 1103 80-81 0.008914740077095473 1103 82-83 0.03048439678480719 1103 84-85 0.1140992245032777 1103 86-87 0.05518285684679114 1103 88-89 0.8375525167467401 1103 90-91 -0.6701628860277951 1103 92-93 -0.36000100723625117 1103 94-95 -0.12330436051888416 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.026878697864546552 1104 2 0.10553802213197372 1104 3 0.11492907625837745 1104 4 0.12337778855830805 1104 5 0.11785566673705716 1104 6 0.15763268741168446 1104 7 0.1633209276644152 1104 8 0.218598236159103 1104 9 0.2813084042226066 1104 10-11 0.271584037720352 1104 12-13 0.30199357023717965 1104 14-15 0.29892321869803595 1104 16-17 0.3113806480808208 1104 18-19 0.2617588321978559 1104 20-21 0.21471417313177454 1104 22-23 0.12535390201848173 1104 24-25 0.23154454402947522 1104 26-27 0.20845499334400586 1104 28-29 0.25737190188129944 1104 30-31 0.22810108963312814 1104 32-33 0.24583896540571715 1104 34-35 0.19923903822962075 1104 36-37 0.1904083151015925 1104 38-39 0.1923939904531906 1104 40-41 0.22818242936287447 1104 42-43 0.23969820736705572 1104 44-45 0.20069566102311853 1104 46-47 0.23083649359496405 1104 48-49 0.15393104779819566 1104 50-51 0.16114703021070653 1104 52-53 0.1469377080205021 1104 54-55 0.22013754245202932 1104 56-57 0.14159248678606673 1104 58-59 0.07747418381903515 1104 60-61 0.1267118088653021 1104 62-63 0.11927903554224883 1104 64-65 0.1712267424505356 1104 66-67 0.17366273295936452 1104 68-69 0.17235035222580564 1104 70-71 0.13273215980906627 1104 72-73 0.18016985262732277 1104 74-75 0.25698231439573505 1104 76-77 0.21082546237484223 1104 78-79 0.17055729880129178 1104 80-81 0.17401318851164405 1104 82-83 0.20907602546541426 1104 84-85 0.2169463559892435 1104 86-87 0.2879923997068161 1104 88-89 0.7335004405368188 1104 90-91 -0.713760962320487 1104 92-93 -0.36000100723625117 1104 94-95 -0.12330436051888416 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.1510639336733135 1105 2 -0.12827849557805848 1105 3 -0.09679087686903642 1105 4 -0.03595642069381455 1105 5 -0.033075564391182866 1105 6 0.06659135278716377 1105 7 -0.004386417948595067 1105 8 -0.04024690442502532 1105 9 -0.03434095254029046 1105 10-11 -0.06497420247305286 1105 12-13 -0.025958788859256288 1105 14-15 -0.08962263001851767 1105 16-17 0.016454399266081055 1105 18-19 0.032432343976523725 1105 20-21 -0.012194711979965689 1105 22-23 -0.14576502959597093 1105 24-25 -0.005351979867469936 1105 26-27 -0.009596352141230113 1105 28-29 0.0035283038221081142 1105 30-31 -0.14201705012384025 1105 32-33 -0.10984048950715675 1105 34-35 -0.05049564327890721 1105 36-37 -0.12200300022244903 1105 38-39 -0.0391360597818462 1105 40-41 0.010821542415243357 1105 42-43 -0.012330086827333275 1105 44-45 -0.01883684464262103 1105 46-47 0.06625360158348315 1105 48-49 0.09210774129722665 1105 50-51 0.021393535782287643 1105 52-53 -0.021746955330783635 1105 54-55 -0.12253363578484056 1105 56-57 0.027382175490245686 1105 58-59 -0.05874905106169592 1105 60-61 -0.03335930279501298 1105 62-63 -0.0690865857380416 1105 64-65 -0.0874006793556461 1105 66-67 0.04522578145937928 1105 68-69 0.0597323721853229 1105 70-71 -0.015982164573415503 1105 72-73 -0.0023500404803158403 1105 74-75 -0.04906345866564976 1105 76-77 0.01773570087357257 1105 78-79 0.024719867479383595 1105 80-81 -0.09602408427065967 1105 82-83 -0.0352252485677198 1105 84-85 -0.05612731617839728 1105 86-87 -0.06076965048114147 1105 88-89 0.6161957275557377 1105 90-91 -0.8445502643426659 1105 92-93 -0.36000100723625117 1105 94-95 -0.12330436051888416 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 0.03342009737181684 1106 2 0.02077594418890527 1106 3 0.07871978104799382 1106 4 0.05043388193759313 1106 5 0.1127482655513461 1106 6 0.0639301318349581 1106 7 0.004717287396331926 1106 8 0.139629068179147 1106 9 0.1312151750288848 1106 10-11 0.04314797909275825 1106 12-13 0.08788680982178221 1106 14-15 0.08201308817716324 1106 16-17 0.050275405886957 1106 18-19 0.011681622261868085 1106 20-21 0.0732720647088172 1106 22-23 0.057217215897793494 1106 24-25 -0.02862768463893417 1106 26-27 -0.1302108766893042 1106 28-29 0.03667199020368628 1106 30-31 0.06951621065789482 1106 32-33 0.09657051150943374 1106 34-35 0.01098389619220086 1106 36-37 0.04494768206670585 1106 38-39 0.04456654816581107 1106 40-41 0.1259680195354207 1106 42-43 -0.15872310199800665 1106 44-45 0.08576758893357095 1106 46-47 0.024354033960612753 1106 48-49 0.1279466571938528 1106 50-51 0.11035443774639475 1106 52-53 -0.013290227255922105 1106 54-55 0.038107541701570824 1106 56-57 -0.07366520678718302 1106 58-59 0.026655614180000953 1106 60-61 0.11155484900292123 1106 62-63 0.18821812772657864 1106 64-65 0.15147831695595215 1106 66-67 0.09644125156768979 1106 68-69 0.09558635774451574 1106 70-71 0.10938806037998461 1106 72-73 0.18966739634113594 1106 74-75 0.09003278515986324 1106 76-77 0.12601143008232896 1106 78-79 0.184517143861882 1106 80-81 0.1081307438863135 1106 82-83 0.21671547959837767 1106 84-85 0.232592552642231 1106 86-87 0.32329393697829545 1106 88-89 0.8255628261650934 1106 90-91 -0.27991688886858235 1106 92-93 -0.35099342190123206 1106 94-95 -0.12330436051888416 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.13811536155834858 1107 2 -0.14490571374973626 1107 3 -0.11274665773963477 1107 4 -0.0912816248962045 1107 5 -0.136266297327019 1107 6 -0.10320882231255268 1107 7 -0.10204159725651607 1107 8 -0.16262796640645405 1107 9 -0.12388728947343708 1107 10-11 -0.0924756717145172 1107 12-13 -0.12573308193039878 1107 14-15 -0.13980689990426498 1107 16-17 -0.1104958726544325 1107 18-19 -0.11777587918049903 1107 20-21 -0.18506109726707365 1107 22-23 -0.09119339107532198 1107 24-25 -0.10298216796262238 1107 26-27 -0.13815210793999455 1107 28-29 -0.06666460617083203 1107 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14522.0 3 2462.0 4 1731.0 5 1438.0 6 1593.0 7 1906.0 8 2259.0 9 2611.0 10 3175.0 11 3762.0 12 4766.0 13 5806.0 14 6738.0 15 7881.0 16 9123.0 17 10763.0 18 13770.0 19 17617.0 20 18381.0 21 26399.0 22 31286.0 23 38022.0 24 58647.0 25 101028.0 26 179474.0 27 532334.0 28 988985.0 29 38918.0 30 1624.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.22409745837018 21.145348353401307 15.243290968918503 23.38726321931001 2 12.40101062719441 21.042869850248895 45.43515831717596 21.120961205380734 3 18.186047058546855 27.058876097973965 30.049598438437027 24.705478405042154 4 11.02721136433124 18.293139537618476 41.301065406053766 29.378583691996518 5 11.681487945020544 39.80709210517032 36.16926597628753 12.342153973521617 6 28.07997664338998 38.70464842613214 20.638113116889773 12.57726181358811 7 26.624325758890016 32.60818769537301 23.839962087821416 16.927524457915556 8 23.000337091171172 39.41230481504414 21.838007241113274 15.749350852671412 9 25.33403541104456 18.01210143769217 22.96682682814454 33.68703632311873 10-11 23.07240969474492 27.222934751538315 31.233309888820354 18.47134566489642 12-13 25.507061042906926 25.118130689531554 30.034497146786244 19.340311120775276 14-15 19.86797964570628 30.18360659899611 26.97672636441445 22.971687390883165 16-17 19.321660334637663 30.92168515813461 30.597898371287112 19.158756135940617 18-19 20.360621708063327 29.184404874270204 31.246460488531923 19.208512929134542 20-21 21.62063938967029 29.034079515971346 29.121104250319057 20.22417684403931 22-23 20.218785789642936 31.101799143587343 29.37106137624047 19.308353690529252 24-25 20.941730241497382 29.231117135185787 30.816385921906743 19.010766701410095 26-27 19.11283433496895 30.683477031961605 30.388251926050565 19.815436707018875 28-29 19.270295848752163 30.760827814211478 28.761391049079005 21.207485287957354 30-31 20.31608996808212 29.213792435523672 29.894791823870097 20.57532577252411 32-33 19.28391026593374 30.57979422622327 30.568581229113732 19.56771427872925 34-35 19.744717902210112 30.61389423087094 30.470430571981122 19.170957294937825 36-37 19.112410164744688 29.97921738809512 30.317437295444304 20.59093515171589 38-39 20.377937030240886 30.719395812265137 29.278836457185896 19.623830700308083 40-41 21.80385148994768 28.752960125922595 30.441848011843796 19.001340372285934 42-43 19.31215065577632 30.918641611906985 30.42595724254721 19.343250489769495 44-45 19.087431109523077 30.823723829258576 29.686803235046256 20.402041826172095 46-47 20.842953595662667 29.197478539233977 29.58539196369006 20.37417590141329 48-49 20.033774984508828 29.6853388170669 29.53113231763906 20.749753880785214 50-51 19.081498350860606 31.090843826699206 29.825222488661947 20.002435333778244 52-53 19.234530359596828 30.459196218561075 30.123797555360287 20.18247586648181 54-55 19.356114174383354 29.45568201478896 30.67009654949337 20.51810726133432 56-57 20.10605443011611 29.61921861608324 28.721625221377696 21.553101732422952 58-59 20.60868228381384 30.103393431470586 28.2276714710386 21.060252813676968 60-61 19.702179715197925 28.901548221667767 30.888223482513805 20.508048580620503 62-63 20.09924210885603 28.89478498209115 30.56853471793055 20.437438191122276 64-65 20.3640212814322 29.99503765482058 29.054918892336858 20.586022171410356 66-67 20.491052979730807 30.792808345568755 28.225978963066183 20.490159711634252 68-69 21.09717634741089 28.871147798945422 30.239521169225693 19.792154684417994 70-71 21.889017550837533 28.77409296852264 29.488472375213977 19.84841710542585 72-73 20.778591278600448 29.939525749863304 29.096327680826846 20.185555290709402 74-75 19.79138898957744 30.78295888945149 30.11159739372578 19.31405472724529 76-77 20.36357421953051 29.107634574364806 30.518974659864668 20.00981654624002 78-79 20.050107815488712 29.041447080613263 29.991111951099512 20.917333152798513 80-81 20.648785319938074 29.810801115738865 29.651681859276422 19.888731705046638 82-83 19.95964282204995 29.871622425314072 30.73713438648768 19.431600366148302 84-85 19.991241263007055 29.456695027171833 30.656355208862735 19.895708500958385 86-87 18.971403801417587 31.120321805964846 28.97809868941926 20.930175703198316 88-89 29.04137110431062 18.6931017268778 35.97630508125858 16.289222087553 90-91 30.789827208163125 16.317897194563443 18.097332409260535 34.794943188012894 92-93 22.418472156427523 18.392230646571193 37.027825049244214 22.16147214775707 94-95 15.952670797188429 7.3383350527276185 28.89370395012024 47.81529019996371 96-97 27.66357445991079 7.367536517673558 16.035229216141747 48.9336598062739 98-99 57.331658750765925 14.033656401385256 18.979542784794702 9.655142063054127 100-101 30.750816062182455 7.160657279138677 13.255491936999658 48.833034721679205 102-103 23.182211257599274 2.0832059898372375 37.938708782041715 36.795873970521775 104-105 40.692471863245174 4.404615606195791 43.13111531346505 11.77179721709398 106-107 5.864573372539871 1.9241368878998952 7.022439504603008 85.18885023495723 108-109 18.886072545130602 12.15046895485259 23.454465996571084 45.508992503445725 110-111 40.657656095727305 2.875348534408228 26.757982263310335 29.709013106554128 112-113 27.082158829922765 2.74068483079559 40.17191056418776 30.00524577509388 114-115 28.748221753965623 3.907684518652419 24.94571670000321 42.39837702737875 116-117 20.058359390387594 4.32501540292582 21.243070435704787 54.373554770981805 118-119 20.867303685370278 4.34309736326222 16.856817360397468 57.93278159097004 120-121 25.696853045443756 4.303961917271697 31.328724799075736 38.67046023820881 122-123 34.56161245813459 1.7256042144676742 35.249557749455576 28.463225577942158 124-125 38.887238148347905 2.1093647684402193 20.985543737505026 38.01785334570685 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 7.0 1 15.0 2 36.0 3 57.5 4 69.0 5 68.5 6 64.5 7 67.5 8 75.5 9 81.0 10 94.0 11 101.5 12 109.0 13 125.5 14 162.0 15 227.0 16 312.0 17 413.5 18 629.0 19 1010.5 20 1578.0 21 2433.0 22 3708.5 23 5541.5 24 8112.5 25 11492.0 26 15726.0 27 21296.0 28 28520.5 29 37176.0 30 47456.0 31 59889.5 32 74401.5 33 90087.0 34 104708.5 35 114853.5 36 118899.5 37 117950.0 38 113345.0 39 107220.0 40 101289.0 41 95035.5 42 88365.5 43 82555.5 44 78190.5 45 73477.5 46 67535.0 47 62127.5 48 57821.0 49 53479.0 50 48597.5 51 43104.5 52 36952.0 53 31071.0 54 25824.0 55 21033.0 56 16979.0 57 13525.5 58 10690.0 59 8294.5 60 6236.0 61 4714.5 62 3448.5 63 2447.5 64 1751.5 65 1251.5 66 892.0 67 596.5 68 422.0 69 310.0 70 228.5 71 172.5 72 127.0 73 98.5 74 63.5 75 43.0 76 36.0 77 22.0 78 11.0 79 8.0 80 7.5 81 7.0 82 6.0 83 6.0 84 6.0 85 6.0 86 5.0 87 4.0 88 4.0 89 3.5 90 4.0 91 4.5 92 3.5 93 2.5 94 2.0 95 1.0 96 2.0 97 2.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 0.0011753527586234457 3 0.04447534838631118 4 0.050352112179428414 5 0.05340802935184937 6 0.0 7 0.0 8 0.0 9 4.7014110344937825E-5 10-11 6.111834344841917E-4 12-13 0.001574972696555417 14-15 2.3507055172468913E-5 16-17 1.880564413797513E-4 18-19 0.006511454282773889 20-21 0.001857057358625044 22-23 4.231269931044405E-4 24-25 0.0 26-27 0.0 28-29 0.0010343104275886321 30-31 0.0 32-33 0.0011753527586234457 34-35 7.052116551740673E-5 36-37 1.1753527586234457E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 1.645493862072824E-4 46-47 0.0 48-49 1.4104233103481346E-4 50-51 1.1753527586234457E-4 52-53 0.0 54-55 0.0013634092000031968 56-57 0.0 58-59 0.0 60-61 0.0 62-63 2.3507055172468913E-5 64-65 1.1753527586234457E-4 66-67 0.0 68-69 3.0559171724209587E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.526058275870337E-4 80-81 0.0 82-83 6.581975448291296E-4 84-85 9.402822068987565E-5 86-87 0.0011753527586234457 88-89 19.336645007266032 90-91 26.26217606690296 92-93 40.35427952991532 94-95 44.811875388160246 96-97 48.35939560540305 98-99 93.0560864232182 100-101 51.59960809037617 102-103 53.081939482496885 104-105 74.86526931327899 106-107 81.66115896363976 108-109 12.591953723070906 110-111 39.122063204829665 112-113 52.00698535651506 114-115 86.81381142922426 116-117 66.46302504770757 118-119 71.77268113478898 120-121 80.38585420642296 122-123 75.84184641336404 124-125 49.92158046394464 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2127021.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.5713498748225 #Duplication Level Percentage of deduplicated Percentage of total 1 81.67876144988989 56.825016901724545 2 12.120709233229066 16.86508205591942 3 3.3054176318679103 6.898870996472691 4 1.240463415751503 3.452028572166609 5 0.5755258028692795 2.0020053496703385 6 0.3046471737147389 1.2716829066530357 7 0.17610822510570334 0.8576460861264023 8 0.11715871715378022 0.652071208159281 9 0.07862774562686539 0.4923214560787678 >10 0.36071965131534467 4.319429671460711 >50 0.02371124415906355 1.1346503770820657 >100 0.014698364619137502 2.0507348030578343 >500 0.002030202763376401 1.053444436308957 >1k 0.0013534685089173016 1.864937991804592 >5k 6.767342544586509E-5 0.2600771873148511 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5524 0.2597059455454366 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 4959 0.2331429732005467 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 4075 0.19158249965562166 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3161 0.14861160280034846 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 3129 0.14710715126931045 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 2700 0.12693809793133212 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2424 0.11396220347612929 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.7014110344937825E-5 0.0 2 0.0 0.0 0.0 4.7014110344937825E-5 0.0 3 0.0 0.0 0.0 1.880564413797513E-4 0.0 4 0.0 0.0 0.0 1.880564413797513E-4 0.0 5 0.0 0.0 0.0 3.2909877241456476E-4 0.0 6 0.0 0.0 0.0 3.761128827595026E-4 0.0 7 0.0 0.0 0.0 3.761128827595026E-4 0.0 8 0.0 0.0 0.0 3.761128827595026E-4 0.0 9 0.0 0.0 0.0 3.761128827595026E-4 0.0 10-11 0.0 0.0 0.0 4.466340482769094E-4 0.0 12-13 0.0 0.0 0.0 5.171552137943161E-4 0.0 14-15 0.0 0.0 0.0 6.346904896566607E-4 0.0 16-17 0.0 0.0 0.0 7.757328206914741E-4 0.0 18-19 0.0 0.0 0.0 7.992398758639431E-4 0.0 20-21 0.0 0.0 0.0 8.22746931036412E-4 0.0 22-23 0.0 0.0 0.0 8.932680965538188E-4 0.0 24-25 0.0 0.0 0.0 0.0011048315931060388 0.0 26-27 0.0 0.0 0.0 0.001316395089658259 0.0 28-29 0.0 0.0 0.0 0.002115634965522202 0.0 30-31 0.0 0.0 0.0 0.003996199379319715 0.0 32-33 0.0 0.0 0.0 0.008580075137951153 0.0 34-35 2.3507055172468913E-5 0.0 0.0 0.01490347297934529 0.0 36-37 4.7014110344937825E-5 0.0 0.0 0.0247999432069547 0.0 38-39 4.7014110344937825E-5 0.0 0.0 0.04442833427596624 0.0 40-41 4.7014110344937825E-5 0.0 0.0 0.06894619282085132 0.0 42-43 4.7014110344937825E-5 0.0 0.0 0.09426329124160035 0.0 44-45 4.7014110344937825E-5 0.0 0.0 0.12418777247615327 0.0 46-47 4.7014110344937825E-5 0.0 0.0 0.1588841859107174 0.0 48-49 4.7014110344937825E-5 0.0 0.0 0.19677755884873727 0.0 50-51 4.7014110344937825E-5 0.0 0.0 0.23951338515228576 0.0 52-53 4.7014110344937825E-5 0.0 0.0 0.2859163120627394 0.0 54-55 4.7014110344937825E-5 0.0 0.0 0.33490501504216463 0.0 56-57 4.7014110344937825E-5 0.0 0.0 0.3926618496009207 0.0 58-59 4.7014110344937825E-5 0.0 0.0 0.45977449211831944 0.0 60-61 4.7014110344937825E-5 0.0 0.0 0.5376298588495365 0.0 62-63 4.7014110344937825E-5 0.0 0.0 0.6194109037945559 0.0 64-65 4.7014110344937825E-5 0.0 0.0 0.6994994407671575 0.0 66-67 4.7014110344937825E-5 0.0 0.0 0.7945149577742767 0.0 68-69 4.7014110344937825E-5 0.0 0.0 0.9090883446848903 0.0 70-71 4.7014110344937825E-5 0.0 0.0 1.039270416230023 0.0 72-73 4.7014110344937825E-5 0.0 0.0 1.1680185574096353 0.0 74-75 4.7014110344937825E-5 0.0 0.0 1.2962260363202809 0.0 76-77 4.7014110344937825E-5 0.0 0.0 1.43484714067233 0.0 78-79 4.7014110344937825E-5 0.0 0.0 1.4745270498034575 0.0 80-81 4.7014110344937825E-5 0.0 0.0 1.475208754403459 0.0 82-83 4.7014110344937825E-5 0.0 0.0 1.4752557685138041 0.0 84-85 4.7014110344937825E-5 0.0 0.0 1.4752557685138041 0.0 86-87 4.7014110344937825E-5 0.0 0.0 1.4752557685138041 0.0 88-89 4.7014110344937825E-5 0.0 0.0 1.4752557685138041 0.0 90-91 4.7014110344937825E-5 0.0 0.0 1.4752557685138041 0.0 92-93 4.7014110344937825E-5 0.0 0.0 1.4752557685138041 0.0 94-95 4.7014110344937825E-5 0.0 0.0 1.4752557685138041 0.0 96-97 4.7014110344937825E-5 0.0 0.0 1.4752557685138041 0.0 98-99 4.7014110344937825E-5 0.0 0.0 1.4752557685138041 0.0 100-101 4.7014110344937825E-5 0.0 0.0 1.4752557685138041 0.0 102-103 4.7014110344937825E-5 0.0 0.0 1.4752557685138041 0.0 104-105 4.7014110344937825E-5 0.0 0.0 1.4752557685138041 0.0 106-107 4.7014110344937825E-5 0.0 0.0 1.4752557685138041 0.0 108-109 4.7014110344937825E-5 0.0 0.0 1.4752557685138041 0.0 110-111 4.7014110344937825E-5 0.0 0.0 1.4752557685138041 0.0 112-113 4.7014110344937825E-5 0.0 0.0 1.4752557685138041 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGCGCG 55 0.0 2664.2107 108-109 CCGCTCC 395 0.0 2349.4514 108-109 CCGCTCG 110 0.0 2220.1755 108-109 CCGCGCA 35 2.8539944E-9 2093.3083 108-109 CCGCGCC 360 0.0 1831.6449 108-109 CGGCGCA 15 0.0030849818 1628.1287 108-109 CCGCGTG 20 0.0034291993 1543.9628 110-111 CGCCGTC 60 9.616997E-9 1543.9626 110-111 CGCCGCT 45 8.398894E-6 1372.4114 110-111 CTCCGCC 295 0.0 1360.7806 110-111 CCTCGCG 155 0.0 1260.4868 108-109 CGTCGCC 215 0.0 1249.494 108-109 TTGTTCG 255 0.0 1245.0397 108-109 CGGCGTT 50 1.1519727E-5 1235.1702 110-111 CGTCGCG 60 2.462366E-8 1221.0966 108-109 CCGCGCG 110 0.0 1110.0878 108-109 CTCGTCG 30 0.007714039 1029.3085 110-111 CGCCGCA 30 0.007714039 1029.3085 110-111 CCGTTCG 50 2.3303768E-5 976.87726 108-109 CATCGCG 75 6.008668E-8 976.8772 108-109 >>END_MODULE