FastQCFastQC Report
Fri 27 May 2016
SRR939962_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939962_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2127021
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT58630.2756437289523705No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA48850.22966392903502128No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40460.19021909045561844No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC38570.1813334236004252No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30700.1443333187589591No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29530.1388326678486014No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA29380.1381274561934273No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA29300.13775134331066782No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT22170.10423028263472715No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG350.00775662651.0672843
GTATCGC650.001940155636.6119776
TACGACG851.6977632E-435.0461774
GTACATG96700.031.7968671
TAGGACC33350.031.620374
TACATGG96900.030.8037432
ACATGGG91850.029.4487063
ATAACGC850.007165461328.0369423
CATGGGG45900.027.6475374
GTATCAA158450.027.2650871
AGAGTAC115550.027.23718610-11
AGTACTT72750.026.29205512-13
GAGTACT69700.025.351312-13
CAACGCA174100.024.5363485
TCAACGC173850.024.5030924
ATCAACG174600.024.431963
AACGCAG175750.024.3394156
TATCAAC179800.023.924182
GTAGGAC111600.023.4363443
TGTAGGA120550.023.4261362
Produced by FastQC (version 0.11.5)