FastQCFastQC Report
Fri 27 May 2016
SRR939961_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939961_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077900
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT125711.1662491882363857No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT75900.704146952407459No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT56730.5263011411077094No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT38780.35977363391780315No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC37850.35114574635866036No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT35940.33342610631784025No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC32870.30494480007421837No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC32520.3016977456164765No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA29860.277020131737638No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG28730.266536784488357No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC26590.24668336580387792No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA23740.2202430652194081No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC17730.16448650153075425No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA17590.16318767974765747No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT17000.1577140736617497No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC16090.14927173207162076No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG15420.14305594210965766No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC13680.12691344280545505No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA13320.12357361536320624No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG12390.11494572780406345No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC12000.11132758140829391No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11810.10956489470266258No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT11230.1041840616012617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCG600.02812.6704108-109
CGTCGCG100.00140537112410.86108-109
CCGCGCT352.9813236E-92066.4517108-109
CGGCGTG150.0019127772065.6812110-111
CCGCTCC3050.01897.0703108-109
CCACGCG458.1454345E-91607.2401108-109
CCGTTCA458.1454345E-91607.2401108-109
CCGCGCC2000.01567.0592108-109
CTCCGCC2000.01394.3348110-111
CGTTGCG601.9599729E-51032.8406110-111
CCGCGAC250.008779946964.3441108-109
CCGTTGC502.4131437E-5964.3441108-109
CGCGTGT652.4916346E-5953.3913110-111
CGGCGCG608.475061E-6803.6201108-109
CCGCTCG901.3013232E-7803.62006108-109
GTCGTCG906.610156E-5688.56036110-111
CGTTGCC957.773255E-5652.32043110-111
CCGTTCC2300.0628.92004108-109
TTGTTCG1551.4533725E-9622.15753108-109
CGGCGCC1651.9863364E-9584.451108-109
Produced by FastQC (version 0.11.5)