FastQCFastQC Report
Fri 27 May 2016
SRR939960_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939960_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562517
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29680.5276284983387168No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16130.2867468894273418No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC10460.18594993573527557No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT9940.17670577067004198No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT9010.1601729369956819No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA9000.15999516459058127No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC8920.1585729853497761No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT6780.12052969065823789No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA6340.11270770483380946No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC6010.10684121546548815No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC5960.10595235343998492No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA5750.10221913293287135No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCG108.7503355E-43049.6992110-111
CAGCGCG201.4919024E-62433.5984108-109
CCGCGCA100.00137586362433.5984108-109
CTCCGCC700.02178.3567110-111
GGTCGCC352.8303475E-92085.9417108-109
CTCGTCG150.0019686132033.1329110-111
CGTCGCG252.913428E-61946.8788108-109
CCGCACG252.913428E-61946.8788108-109
AATCGCG150.00309527321622.399108-109
CGTCGCT200.0034993791524.8496110-111
CGCGTTC200.0034993791524.8496110-111
CGGTTGT250.005467191219.8796110-111
GTTCGCG250.00109813011219.8796110-111
CATCGTA250.005467191219.8796110-111
CGCGTCG250.005467191219.8796110-111
TCGTTCG200.00550196231216.7992108-109
CCGCTCA200.00550196231216.7992108-109
GGACGGC401.192797E-51216.7992108-109
CGTGGCC653.3629476E-81123.1993108-109
CCTCGCG451.69808E-51081.5994108-109
Produced by FastQC (version 0.11.5)