##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939960_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 562517 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.602428015508863 33.0 31.0 34.0 28.0 34.0 2 31.722275060131516 34.0 31.0 34.0 28.0 34.0 3 31.877054382356445 34.0 31.0 34.0 28.0 34.0 4 35.01379513863581 37.0 35.0 37.0 32.0 37.0 5 35.090886142107706 37.0 35.0 37.0 32.0 37.0 6 35.11536718001412 37.0 36.0 37.0 32.0 37.0 7 35.033271883338635 37.0 36.0 37.0 32.0 37.0 8 35.02555833868132 37.0 37.0 37.0 32.0 37.0 9 36.5569218352512 39.0 38.0 39.0 32.0 39.0 10-11 36.54342090994584 39.0 38.0 39.0 32.0 39.0 12-13 36.46710321643613 39.0 38.0 39.0 32.0 39.0 14-15 37.96180204331603 41.0 39.0 41.0 32.0 41.0 16-17 38.011817420629065 41.0 39.0 41.0 32.0 41.0 18-19 38.059455980885915 41.0 39.0 41.0 32.0 41.0 20-21 38.14412542198725 41.0 39.0 41.0 32.0 41.0 22-23 38.17467205435569 41.0 39.0 41.0 32.5 41.0 24-25 38.23568887695839 41.0 39.0 41.0 33.0 41.0 26-27 38.22395234277364 41.0 39.0 41.0 33.0 41.0 28-29 38.18678102172912 41.0 39.0 41.0 33.0 41.0 30-31 38.1119406169058 40.5 38.0 41.0 33.0 41.0 32-33 38.00004088765317 40.0 38.0 41.0 32.0 41.0 34-35 37.92946613169024 40.0 38.0 41.0 32.0 41.0 36-37 38.09582732610748 41.0 38.0 41.0 33.0 41.0 38-39 38.04165029679103 41.0 38.0 41.0 33.0 41.0 40-41 37.97321858717159 41.0 38.0 41.0 32.0 41.0 42-43 37.91860690432467 41.0 38.0 41.0 32.0 41.0 44-45 37.83395612932587 41.0 38.0 41.0 32.0 41.0 46-47 37.7276766746605 40.0 38.0 41.0 32.0 41.0 48-49 37.633974617656 40.0 38.0 41.0 31.0 41.0 50-51 37.51805989863418 40.0 38.0 41.0 31.0 41.0 52-53 37.44282128362343 40.0 38.0 41.0 31.0 41.0 54-55 37.29510219246707 40.0 37.5 41.0 30.5 41.0 56-57 37.078638512258294 40.0 37.0 41.0 30.0 41.0 58-59 36.92059084436559 40.0 37.0 41.0 30.5 41.0 60-61 36.69284306074306 40.0 36.0 41.0 30.0 41.0 62-63 36.47222395056505 40.0 36.0 41.0 30.0 41.0 64-65 36.192066195332764 39.0 35.0 41.0 30.0 41.0 66-67 35.85950380166288 39.0 35.0 41.0 29.5 41.0 68-69 35.437632995980564 38.5 35.0 40.5 29.0 41.0 70-71 35.00032087919121 37.5 35.0 40.0 28.5 41.0 72-73 34.6062945653198 37.0 34.0 39.5 28.0 41.0 74-75 34.18626281516825 36.5 34.0 39.0 28.0 41.0 76-77 33.699123759815265 36.0 34.0 39.0 26.0 40.5 78-79 33.18499174247178 35.5 34.0 38.5 26.0 39.5 80-81 32.69496655212198 35.0 33.5 37.0 25.0 39.0 82-83 32.43929961227839 35.0 34.0 37.0 24.5 39.0 84-85 31.959913211511832 35.0 34.0 36.5 21.0 39.0 86-87 31.25681268299447 35.0 34.0 36.0 2.0 37.0 88-89 15.89578803840595 18.0 16.5 18.5 2.0 19.5 90-91 2.7638711363389907 2.0 2.0 2.0 2.0 2.0 92-93 2.2962177143090785 2.0 2.0 2.0 2.0 2.0 94-95 2.098040592550981 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18520516233239093 1101 2 0.22564657923653186 1101 3 0.35808133603220327 1101 4 0.304207784038077 1101 5 0.3253470003056904 1101 6 0.40336944257293794 1101 7 0.4547193260233229 1101 8 0.21195933689630664 1101 9 0.258237589065331 1101 10-11 0.20361218019946392 1101 12-13 0.39691933728661155 1101 14-15 0.27401002655707885 1101 16-17 0.2785589129713344 1101 18-19 0.3503524596033998 1101 20-21 0.4774532122110742 1101 22-23 0.15115048973957101 1101 24-25 0.26283081343542136 1101 26-27 0.38381233793883496 1101 28-29 0.48514705376666 1101 30-31 0.3561675664058228 1101 32-33 0.41224392611102445 1101 34-35 0.4087176944485975 1101 36-37 0.5427277029754123 1101 38-39 0.41643903232769475 1101 40-41 0.3387305447470652 1101 42-43 0.4008194573944621 1101 44-45 0.41156623447817964 1101 46-47 0.3825328763301741 1101 48-49 0.44731523146000285 1101 50-51 0.4133524562431532 1101 52-53 0.37135067176519954 1101 54-55 0.43278304628785946 1101 56-57 0.3746653349826019 1101 58-59 0.40212948074331223 1101 60-61 0.46913705880751166 1101 62-63 0.3135456923218314 1101 64-65 0.39540986889704044 1101 66-67 0.23681787950186362 1101 68-69 0.3471399329575462 1101 70-71 0.4334667258127709 1101 72-73 0.2597458907375625 1101 74-75 0.40874019686301466 1101 76-77 0.3840843812377912 1101 78-79 0.3254699475952876 1101 80-81 0.1825816585589024 1101 82-83 0.10474270284575482 1101 84-85 -0.05408405711219899 1101 86-87 0.043734311174418394 1101 88-89 0.6321156447710976 1101 90-91 -0.15035011632833806 1101 92-93 -0.16567143178683041 1101 94-95 0.007967836669062311 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.21420204601806603 1102 2 0.2814639800619325 1102 3 0.2228080268432855 1102 4 0.12155639011910324 1102 5 0.2847338444057499 1102 6 0.24920789957621992 1102 7 0.2713065407121107 1102 8 0.2584058963168374 1102 9 0.45491239745410894 1102 10-11 0.42302008047740713 1102 12-13 0.4129051875350669 1102 14-15 0.4092976539469504 1102 16-17 0.40196748704696716 1102 18-19 0.3576471618690462 1102 20-21 0.40237025087022005 1102 22-23 0.44009683859832194 1102 24-25 0.37507119076213513 1102 26-27 0.17885655591238248 1102 28-29 0.224813523908999 1102 30-31 0.3207822383505672 1102 32-33 0.23846307881042605 1102 34-35 0.28297525361645626 1102 36-37 0.27112322222075136 1102 38-39 0.21033189849462275 1102 40-41 0.35570350861466693 1102 42-43 0.25247447297603287 1102 44-45 0.31080400083883575 1102 46-47 0.30958248468527216 1102 48-49 0.2904652356712347 1102 50-51 0.23072127625493977 1102 52-53 0.23486115774178273 1102 54-55 0.204690736004018 1102 56-57 0.2145760567883812 1102 58-59 0.08450377536135534 1102 60-61 0.08790906251339692 1102 62-63 0.2665348273340413 1102 64-65 0.2742248272058063 1102 66-67 0.3820802394782845 1102 68-69 0.1914707676244376 1102 70-71 0.22334459998105416 1102 72-73 0.08364296814007588 1102 74-75 0.07476142149031517 1102 76-77 0.09184316166396655 1102 78-79 0.26662298389614136 1102 80-81 0.2888345011429152 1102 82-83 0.10738146305799745 1102 84-85 0.09134759453439045 1102 86-87 0.22245746454761672 1102 88-89 0.9472137665600329 1102 90-91 0.20406410080296844 1102 92-93 -0.0835877124235691 1102 94-95 0.048511928305548846 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.005942374169769948 1103 2 0.058750862816815186 1103 3 0.074791740166404 1103 4 -0.023999248946701357 1103 5 -0.15196738461093418 1103 6 -0.0986322530950332 1103 7 -0.09223372012306896 1103 8 0.05316729846116175 1103 9 -0.19033280134203068 1103 10-11 -0.11909604379020067 1103 12-13 0.1009404927632076 1103 14-15 -0.06142701167620146 1103 16-17 -0.09451064978856749 1103 18-19 -0.06566837288882965 1103 20-21 -0.048784423543288824 1103 22-23 0.030515017980512482 1103 24-25 -0.1192338641857944 1103 26-27 -0.08866616341366296 1103 28-29 -0.0017849983953155402 1103 30-31 -0.07130790696815126 1103 32-33 -0.3045149256795199 1103 34-35 -0.3019415974081596 1103 36-37 -0.14964098368937329 1103 38-39 -0.1678227465087545 1103 40-41 -0.16356829654648664 1103 42-43 -0.13232277684996063 1103 44-45 -0.15290144689773655 1103 46-47 -0.20798026509660872 1103 48-49 -0.41702487108754127 1103 50-51 -0.30414279992383086 1103 52-53 -0.24278877673898336 1103 54-55 -0.2923943932535096 1103 56-57 -0.2648727973602405 1103 58-59 -0.43186704717464863 1103 60-61 -0.38331802617775423 1103 62-63 -0.43777571750496946 1103 64-65 -0.30979583177719405 1103 66-67 -0.3385545618562844 1103 68-69 -0.30641006906110135 1103 70-71 -0.29196990869002803 1103 72-73 -0.4310514769397713 1103 74-75 -0.35090855762848605 1103 76-77 -0.38673155804114856 1103 78-79 -0.4254210855509797 1103 80-81 -0.49352072748817477 1103 82-83 -0.24764374795968536 1103 84-85 -0.34328103720828906 1103 86-87 -0.4342446140950713 1103 88-89 0.20369624955934285 1103 90-91 -0.5910469551187614 1103 92-93 -0.31857465759328196 1103 94-95 -0.10945151816964716 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.10615960193559815 1104 2 -0.025172279324262803 1104 3 0.11093493404709065 1104 4 -0.024129684944554697 1104 5 0.0072328563850945216 1104 6 0.08212874778385526 1104 7 -0.14270002881538346 1104 8 -0.02982726359997656 1104 9 0.10146339551694439 1104 10-11 -0.12187169076828042 1104 12-13 0.04654712884988044 1104 14-15 0.09341449305831873 1104 16-17 -0.09295473467134485 1104 18-19 0.03325568540985557 1104 20-21 -0.09155423195765877 1104 22-23 -0.2253755648509994 1104 24-25 -0.18089127093181645 1104 26-27 -0.2119444858825119 1104 28-29 0.023385267166155188 1104 30-31 -0.07604087031874229 1104 32-33 -0.14929453542743687 1104 34-35 -0.08406146187894592 1104 36-37 -0.14861224739679813 1104 38-39 -0.15480166246138793 1104 40-41 -0.18119501356559198 1104 42-43 -0.07821280067005887 1104 44-45 -0.2716843858692144 1104 46-47 -0.27947704922814154 1104 48-49 -0.237126455884912 1104 50-51 -0.36811156360796815 1104 52-53 -0.3977312140908751 1104 54-55 -0.3570184425458862 1104 56-57 -0.293175856084396 1104 58-59 -0.39469434059662944 1104 60-61 -0.4422028915646976 1104 62-63 -0.2447917766607972 1104 64-65 -0.2693047713014707 1104 66-67 -0.36132107831451066 1104 68-69 -0.2840263201441928 1104 70-71 -0.23546627666861042 1104 72-73 -0.2974997668306685 1104 74-75 -0.545676676586119 1104 76-77 -0.5615235592088652 1104 78-79 -0.5122223600970202 1104 80-81 -0.5210411702252173 1104 82-83 -0.34592727234034726 1104 84-85 -0.39078380897324294 1104 86-87 -0.5467619667412116 1104 88-89 0.06142085568921374 1104 90-91 -0.5894071882886358 1104 92-93 -0.31857465759328196 1104 94-95 -0.10945151816964716 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.18471261122864746 1105 2 -0.05853115637384221 1105 3 0.053124030529357213 1105 4 0.022256487390386326 1105 5 0.022990896573880093 1105 6 0.011974755665917769 1105 7 6.427921839815554E-4 1105 8 -0.06346286424221859 1105 9 -0.014879109226889398 1105 10-11 0.014039125171002809 1105 12-13 0.006525599146193883 1105 14-15 0.001972550276235552 1105 16-17 0.06065726844255437 1105 18-19 0.009516754353555257 1105 20-21 0.18116447090810084 1105 22-23 -0.04392699286638191 1105 24-25 -0.006371431978870135 1105 26-27 0.0623037990393982 1105 28-29 0.02069101328594769 1105 30-31 -0.009394577806709492 1105 32-33 -0.058308730435463474 1105 34-35 0.11671975010957425 1105 36-37 -0.107257274886706 1105 38-39 0.02053770405444766 1105 40-41 0.16232083570215394 1105 42-43 0.11853767371324864 1105 44-45 0.181996342005057 1105 46-47 0.17863581750664537 1105 48-49 0.20446814101091348 1105 50-51 0.031028768703620813 1105 52-53 -0.018002585666799575 1105 54-55 0.09250078822334018 1105 56-57 0.012296581029403342 1105 58-59 0.04630879763136164 1105 60-61 -0.028773282748467466 1105 62-63 0.1310733646810931 1105 64-65 0.10298577022530964 1105 66-67 0.08599956830641986 1105 68-69 0.19292503732187072 1105 70-71 0.16176131784313696 1105 72-73 0.0848668584795007 1105 74-75 0.056879823681484254 1105 76-77 0.15799883411570903 1105 78-79 0.2535265573359595 1105 80-81 -0.001851453395559588 1105 82-83 0.04784279772241007 1105 84-85 0.19980667660063745 1105 86-87 0.2210927868227408 1105 88-89 0.48083955368950626 1105 90-91 -0.759382374392854 1105 92-93 -0.31857465759328196 1105 94-95 -0.10945151816964716 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.3657843178896485 1106 2 -0.28155267545078644 1106 3 -0.2277344319892265 1106 4 -0.32662331723904714 1106 5 -0.197163771791665 1106 6 -0.14845385621205054 1106 7 -0.35278504104316966 1106 8 -0.2116763915303892 1106 9 -0.5030356534330238 1106 10-11 -0.44797510290289466 1106 12-13 -0.4975490451519633 1106 14-15 -0.35097968657517953 1106 16-17 -0.46495414940978463 1106 18-19 -0.4040817626705788 1106 20-21 -0.4717032646337671 1106 22-23 -0.35816126058067965 1106 24-25 -0.43989190695586444 1106 26-27 -0.37714725213919564 1106 28-29 -0.20314454982015917 1106 30-31 -0.37843745669110973 1106 32-33 -0.2824340755193404 1106 34-35 -0.35591023148595013 1106 36-37 -0.30701881307598455 1106 38-39 -0.5736436507979619 1106 40-41 -0.4149226519456093 1106 42-43 -0.7712080260106831 1106 44-45 -0.4362347049263491 1106 46-47 -0.6085381224664559 1106 48-49 -0.5573996476207768 1106 50-51 -0.5098127935530528 1106 52-53 -0.5341374692231184 1106 54-55 -0.4526631708621025 1106 56-57 -0.6930991151551993 1106 58-59 -0.3572137124557031 1106 60-61 -0.37072707690150963 1106 62-63 -0.36119985327034243 1106 64-65 -0.4588683035341532 1106 66-67 -0.3384160011704722 1106 68-69 -0.41972936559452734 1106 70-71 -0.20526857173002355 1106 72-73 -0.1933747179344607 1106 74-75 -0.41425037027335776 1106 76-77 -0.27743729882121926 1106 78-79 -0.08577495749379693 1106 80-81 -0.07712176910306567 1106 82-83 -0.09617884523079567 1106 84-85 -0.3350622800072749 1106 86-87 -0.37296690384052766 1106 88-89 0.3326171780966689 1106 90-91 -0.2545688966551882 1106 92-93 -0.28367694399520715 1106 94-95 -0.10945151816964716 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 0.10731909507089554 1107 2 0.08962278605419272 1107 3 0.25786780747275984 1107 4 0.2387597553642422 1107 5 0.23824260009216403 1107 6 0.13652280183473664 1107 7 0.09355407017035589 1107 8 0.23638242848480218 1107 9 0.3263547247322265 1107 10-11 0.25828540524332055 1107 12-13 0.25383995041861596 1107 14-15 0.37064085092983134 1107 16-17 0.45892419932838635 1107 18-19 0.5161352705543649 1107 20-21 0.447043694938138 1107 22-23 0.535556041482117 1107 24-25 0.5627644670615979 1107 26-27 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-0.569422172208462 2216 42-43 -0.42891902407103544 2216 44-45 -0.4615664368349641 2216 46-47 -0.5240445615283349 2216 48-49 -0.4907702554949154 2216 50-51 -0.5877441945925952 2216 52-53 -0.6246858421433927 2216 54-55 -0.5311249287938651 2216 56-57 -0.6465485057624818 2216 58-59 -0.4753774581328969 2216 60-61 -0.5558688006879606 2216 62-63 -0.6406398378083153 2216 64-65 -0.7660179976298238 2216 66-67 -0.8829964670101944 2216 68-69 -0.7021760480453452 2216 70-71 -0.6242758264600923 2216 72-73 -0.7040313250769046 2216 74-75 -0.6331848015950143 2216 76-77 -0.5762209293154754 2216 78-79 -0.6733822080822591 2216 80-81 -0.740150716695517 2216 82-83 -0.7325495934596802 2216 84-85 -0.8036671077505773 2216 86-87 -0.6584565233410942 2216 88-89 -1.2465181676016872 2216 90-91 -0.759382374392854 2216 92-93 -0.31857465759328196 2216 94-95 -0.10945151816964716 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6784.0 3 1232.0 4 916.0 5 884.0 6 819.0 7 792.0 8 881.0 9 1031.0 10 1213.0 11 1575.0 12 2049.0 13 2343.0 14 2687.0 15 3192.0 16 3565.0 17 4261.0 18 5375.0 19 6676.0 20 6070.0 21 9140.0 22 10023.0 23 11556.0 24 17983.0 25 31002.0 26 53474.0 27 142755.0 28 225429.0 29 8489.0 30 321.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.53809218210294 20.19565630905377 14.924526725414522 22.341724783428766 2 12.952125295550301 20.855451458640736 44.812003342162804 21.380419903646157 3 19.370317082323314 27.640885461591402 28.8420599591309 24.146737496954383 4 11.20747152911296 17.733854343599987 39.88432006915184 31.17435405813521 5 11.693153218849769 39.912492107392815 34.095529449429506 14.298825224327905 6 28.03559714639735 37.371492772662876 20.966299685165072 13.626610395774705 7 27.941733316504212 32.623014060019514 22.23577242998878 17.199480193487485 8 24.5797015912408 37.73414847906819 20.786394011203218 16.899755918487795 9 26.125965970806213 19.130977374906003 22.256571801385558 32.48648485290222 10-11 23.70467553223167 27.447341263809665 29.70248714030863 19.14549606365003 12-13 26.317988284548584 25.399418671834027 28.7746775584217 19.50791548519569 14-15 20.697952239665646 29.97518297224795 26.462044702648985 22.86482008543742 16-17 19.805782956898927 31.056149613788076 29.592455312302786 19.545612117010215 18-19 20.65295172331644 29.540167455884824 30.518252762559854 19.288628058238878 20-21 22.34700135998791 28.804455071510475 28.213171438476103 20.635372130025512 22-23 20.601727780755482 31.002100394034986 28.542603866396092 19.853567958813436 24-25 21.27171267712798 29.39724488326575 30.085668522018 19.245373917588267 26-27 19.68838275109908 30.472234616909354 29.827098558799115 20.012284073192454 28-29 19.33875012110885 31.258616505825227 28.05040972568512 21.352223647380804 30-31 20.529512885832784 29.157963226000284 29.39742265567085 20.915101232496085 32-33 19.486105543294013 30.396696227701447 30.157855392407402 19.959342836597134 34-35 20.112050146129175 30.610773737991455 29.83852903739627 19.4386470784831 36-37 19.518982998720926 29.718506034934087 29.903478386856207 20.85903257948878 38-39 20.406405495300586 30.902443837252918 28.805440546685702 19.885710120760795 40-41 21.891249508903734 29.00134573710661 29.78452206777751 19.32288268621215 42-43 19.459323007126898 30.908488099026343 29.68043632459108 19.951752569255685 44-45 19.536510961465986 30.634239737180806 28.980153453412882 20.849095847940326 46-47 21.170471292423162 29.511641425948014 28.950502829247824 20.367384452380996 48-49 20.306870998450712 29.879656863398672 29.023593085713927 20.78987905243668 50-51 18.947444208303224 31.25348323115855 29.54997764510019 20.249094915438036 52-53 19.21924137403847 30.602008472632825 29.655459301674437 20.52329085165426 54-55 19.283708601736684 29.55030901755262 30.013146468008927 21.152835912701764 56-57 20.368539973014148 29.74034562511 28.082617947546474 21.80849645432938 58-59 20.970921767697686 30.260063251421737 27.663519502521698 21.105495478358876 60-61 19.887932275824554 28.980190820899637 30.74831516203066 20.383561741245153 62-63 20.06614911193795 29.115297848776127 30.118823075569274 20.69972996371665 64-65 20.396379483979583 30.384086511240117 28.348679549844313 20.870854454935987 66-67 20.54240138520258 31.052928178170614 27.599699209090574 20.804971227536235 68-69 21.10790032968039 29.07267279332355 29.681190440424203 20.13823643657186 70-71 22.032134139945995 29.010501015969297 28.825617714664624 20.131747129420088 72-73 20.841592342986967 30.04646970669331 28.875305101890252 20.23663284842947 74-75 19.97246305444991 31.07346089095974 29.506574912402648 19.447501142187704 76-77 20.51458000380433 29.241338483992486 29.882652435393066 20.361429076810122 78-79 20.237131243494623 29.277415466773803 29.519275468853746 20.966177820877824 80-81 20.842747863620122 30.00327101225385 29.143297002579477 20.01068412154655 82-83 20.282553469875364 29.8212663529554 30.356009550001513 19.540170627167726 84-85 20.005724276532334 29.912455901293562 30.137871511324555 19.94394831084955 86-87 19.061383906746382 31.41176407844141 28.399101169936834 21.127750844875372 88-89 29.32943784179193 19.14706802568204 35.14623516606516 16.377258966460875 90-91 30.666011044343687 15.720111937870143 17.5087315877389 36.10514543004727 92-93 22.4484870515569 17.88766743291138 36.52082194270595 23.143023572825776 94-95 16.076335951599376 7.148687351988009 28.56142407055312 48.2135526258595 96-97 27.63595354723186 7.458441301297338 15.741255671008341 49.16434948046246 98-99 57.63948442919862 14.023321002982323 18.665762156488793 9.67143241133027 100-101 31.020266370617623 7.15682634444375 12.93731872203862 48.885588562900004 102-103 23.35328546339391 2.1325261444974224 37.5689884725241 36.94519991958457 104-105 41.31104516466764 4.4991351898161085 42.212287025017595 11.977532620498653 106-107 5.939494461580244 2.0301072765718056 6.968612388213156 85.0617858736348 108-109 18.569451238549885 11.581664356388318 22.723884277937163 47.125000127124636 110-111 40.57904324490938 2.8090273364858014 26.061474589616722 30.550454828988098 112-113 27.51624730756255 2.8116276224618795 39.63230850791697 30.039816562058597 114-115 29.173287726574916 3.984755851221417 24.560316733550593 42.28163968865307 116-117 20.23183638823311 4.352900607443169 20.985967797554313 54.42929520676941 118-119 20.93609042729485 4.399524000416657 16.435242908142815 58.229142664145684 120-121 25.87892486588056 4.27775205831359 30.843386618011493 38.99993645779436 122-123 34.612520002654726 1.7292617637732564 34.774753515673964 28.883464717898043 124-125 39.010659980560064 2.207161502525948 20.541475125231226 38.240703391682764 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 24.0 1 68.0 2 159.0 3 237.5 4 279.5 5 271.0 6 224.5 7 177.5 8 142.0 9 108.0 10 97.5 11 100.0 12 101.5 13 104.5 14 120.5 15 148.0 16 186.5 17 237.5 18 304.5 19 451.0 20 640.0 21 905.5 22 1282.5 23 1760.0 24 2363.0 25 3187.5 26 4281.5 27 5608.0 28 7207.5 29 9062.5 30 11497.5 31 14490.0 32 18069.0 33 21944.0 34 25397.5 35 28032.5 36 29243.5 37 28986.0 38 27894.5 39 26585.0 40 25046.5 41 23241.5 42 21693.0 43 21019.0 44 20694.0 45 19882.5 46 18560.0 47 17212.5 48 16252.5 49 15497.5 50 14543.0 51 13188.5 52 11634.0 53 9790.5 54 8125.5 55 6930.5 56 5800.0 57 4764.0 58 3872.0 59 3104.5 60 2419.0 61 1854.0 62 1413.0 63 1041.0 64 742.0 65 559.5 66 428.0 67 308.0 68 228.0 69 170.0 70 128.5 71 99.5 72 77.0 73 55.5 74 44.0 75 38.0 76 27.5 77 18.5 78 10.5 79 4.5 80 3.0 81 4.0 82 4.5 83 2.5 84 1.5 85 1.0 86 0.5 87 1.5 88 2.5 89 2.0 90 1.5 91 1.5 92 0.5 93 0.5 94 1.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 0.001244406835704521 3 0.04159874279355113 4 0.04906518380777826 5 0.05013181823838213 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.222034178522605E-4 12-13 0.00213326886120775 14-15 0.0 16-17 3.555448102012917E-4 18-19 0.007199782406576157 20-21 0.00213326886120775 22-23 6.222034178522605E-4 24-25 0.0 26-27 0.0 28-29 0.0011555206331541978 30-31 0.0 32-33 0.0015110654433554897 34-35 1.7777240510064585E-4 36-37 8.888620255032293E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 1.7777240510064585E-4 46-47 0.0 48-49 8.888620255032293E-5 50-51 8.888620255032293E-5 52-53 0.0 54-55 0.0015110654433554897 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 8.888620255032293E-5 66-67 0.0 68-69 4.444310127516146E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.6665860765096873E-4 80-81 0.0 82-83 7.110896204025834E-4 84-85 8.888620255032293E-5 86-87 0.0014221792408051668 88-89 19.31657176583108 90-91 26.247028978679754 92-93 40.33611428632379 94-95 44.78842417206947 96-97 48.374449127759696 98-99 93.05558765335093 100-101 51.64110595768662 102-103 53.13368307091163 104-105 74.87000392877016 106-107 81.67202057893361 108-109 12.599352552900623 110-111 39.15357224759429 112-113 52.04820476536709 114-115 86.79897674203623 116-117 66.49079050055377 118-119 71.84022882863984 120-121 80.41605853689755 122-123 75.89281746151671 124-125 49.97848953898282 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 562517.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.35684599682861 #Duplication Level Percentage of deduplicated Percentage of total 1 90.27924088876206 71.64275815920082 2 7.03053551588174 11.158422484181225 3 1.3256042238840786 3.1558731074254296 4 0.4736991219886961 1.5036507308995952 5 0.24643717914994007 0.9778238636847326 6 0.14001125153141677 0.6666510795361124 7 0.09322176412589724 0.5178449625504056 8 0.0611910643115228 0.388474389356124 9 0.050069528735540575 0.357602389290007 >10 0.26055511005139104 3.7796618372547046 >50 0.02150966003737059 1.223483533229315 >100 0.014787870520800871 2.237686915010713 >500 0.0024646450867696628 1.3895528995384123 >1k 6.721759327553626E-4 1.0005136488423092 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2968 0.5276284983387168 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1613 0.2867468894273418 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1046 0.18594993573527557 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 994 0.17670577067004198 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 901 0.1601729369956819 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 900 0.15999516459058127 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 892 0.1585729853497761 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 678 0.12052969065823789 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 634 0.11270770483380946 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 601 0.10684121546548815 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 596 0.10595235343998492 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 575 0.10221913293287135 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.7777240510064585E-4 0.0 10-11 0.0 0.0 0.0 1.7777240510064585E-4 0.0 12-13 0.0 0.0 0.0 1.7777240510064585E-4 0.0 14-15 0.0 0.0 0.0 1.7777240510064585E-4 0.0 16-17 0.0 0.0 0.0 1.7777240510064585E-4 0.0 18-19 0.0 0.0 0.0 2.666586076509688E-4 0.0 20-21 0.0 0.0 0.0 3.555448102012917E-4 0.0 22-23 0.0 0.0 0.0 3.555448102012917E-4 0.0 24-25 0.0 0.0 0.0 3.555448102012917E-4 0.0 26-27 0.0 0.0 0.0 4.4443101275161467E-4 0.0 28-29 0.0 0.0 0.0 9.777482280535522E-4 0.0 30-31 0.0 0.0 0.0 0.0028443584816103336 0.0 32-33 0.0 0.0 0.0 0.007555327216777449 0.0 34-35 8.888620255032293E-5 0.0 0.0 0.013244044179998115 0.0 36-37 1.7777240510064585E-4 0.0 0.0 0.024088160891137512 0.0 38-39 1.7777240510064585E-4 0.0 0.0 0.04346535304710791 0.0 40-41 1.7777240510064585E-4 0.0 0.0 0.07039787241985576 0.0 42-43 3.555448102012917E-4 0.0 0.0 0.09750816419770425 0.0 44-45 3.555448102012917E-4 0.0 0.0 0.127996131672465 0.0 46-47 4.4443101275161467E-4 0.0 0.0 0.16328395408494323 0.0 48-49 5.333172153019376E-4 0.0 0.0 0.20274942801728657 0.0 50-51 5.333172153019376E-4 0.0 0.0 0.24408151220318675 0.0 52-53 5.333172153019376E-4 0.0 0.0 0.2865691170222411 0.0 54-55 5.333172153019376E-4 0.0 0.0 0.3324343975382077 0.0 56-57 5.333172153019376E-4 0.0 0.0 0.39065486020866924 0.0 58-59 5.333172153019376E-4 0.0 0.0 0.4538529502219488 0.0 60-61 5.333172153019376E-4 0.0 0.0 0.5303839706177769 0.0 62-63 5.333172153019376E-4 0.0 0.0 0.6150925216482346 0.0 64-65 5.333172153019376E-4 0.0 0.0 0.6940234695129214 0.0 66-67 5.333172153019376E-4 0.0 0.0 0.7845096237091501 0.0 68-69 5.333172153019376E-4 0.0 0.0 0.9024616144934287 0.0 70-71 5.333172153019376E-4 0.0 0.0 1.032502128824551 0.0 72-73 5.333172153019376E-4 0.0 0.0 1.1595205122689625 0.0 74-75 5.333172153019376E-4 0.0 0.0 1.2902721162204875 0.0 76-77 5.333172153019376E-4 0.0 0.0 1.429379023211743 0.0 78-79 5.333172153019376E-4 0.0 0.0 1.4684000661313348 0.0 80-81 5.333172153019376E-4 0.0 0.0 1.4691111557517373 0.0 82-83 5.333172153019376E-4 0.0 0.0 1.4691111557517373 0.0 84-85 5.333172153019376E-4 0.0 0.0 1.4691111557517373 0.0 86-87 5.333172153019376E-4 0.0 0.0 1.4691111557517373 0.0 88-89 5.333172153019376E-4 0.0 0.0 1.4691111557517373 0.0 90-91 5.333172153019376E-4 0.0 0.0 1.4691111557517373 0.0 92-93 5.333172153019376E-4 0.0 0.0 1.4691111557517373 0.0 94-95 5.333172153019376E-4 0.0 0.0 1.4691111557517373 0.0 96-97 5.333172153019376E-4 0.0 0.0 1.4691111557517373 0.0 98-99 5.333172153019376E-4 0.0 0.0 1.4691111557517373 0.0 100-101 5.333172153019376E-4 0.0 0.0 1.4691111557517373 0.0 102-103 5.333172153019376E-4 0.0 0.0 1.4691111557517373 0.0 104-105 5.333172153019376E-4 0.0 0.0 1.4691111557517373 0.0 106-107 5.333172153019376E-4 0.0 0.0 1.4691111557517373 0.0 108-109 5.333172153019376E-4 0.0 0.0 1.4691111557517373 0.0 110-111 5.333172153019376E-4 0.0 0.0 1.4691111557517373 0.0 112-113 5.333172153019376E-4 0.0 0.0 1.4691111557517373 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTGCG 10 8.7503355E-4 3049.6992 110-111 CAGCGCG 20 1.4919024E-6 2433.5984 108-109 CCGCGCA 10 0.0013758636 2433.5984 108-109 CTCCGCC 70 0.0 2178.3567 110-111 GGTCGCC 35 2.8303475E-9 2085.9417 108-109 CTCGTCG 15 0.001968613 2033.1329 110-111 CGTCGCG 25 2.913428E-6 1946.8788 108-109 CCGCACG 25 2.913428E-6 1946.8788 108-109 AATCGCG 15 0.0030952732 1622.399 108-109 CGTCGCT 20 0.003499379 1524.8496 110-111 CGCGTTC 20 0.003499379 1524.8496 110-111 CGGTTGT 25 0.00546719 1219.8796 110-111 GTTCGCG 25 0.0010981301 1219.8796 110-111 CATCGTA 25 0.00546719 1219.8796 110-111 CGCGTCG 25 0.00546719 1219.8796 110-111 TCGTTCG 20 0.0055019623 1216.7992 108-109 CCGCTCA 20 0.0055019623 1216.7992 108-109 GGACGGC 40 1.192797E-5 1216.7992 108-109 CGTGGCC 65 3.3629476E-8 1123.1993 108-109 CCTCGCG 45 1.69808E-5 1081.5994 108-109 >>END_MODULE