FastQCFastQC Report
Fri 27 May 2016
SRR939960_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939960_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562517
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33670.5985596879738746No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24890.4424755162955075No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17960.31927923956075993No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16600.2951021924670721No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11810.20994921042386275No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC10660.18950538383728846No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT9340.16603942636400323No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA9200.1635506126925942No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT9100.16177288864158773No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC8320.14790664104373732No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC6980.1240851387602508No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC6550.11644092534092304No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT6430.11430765647971529No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA6330.11252993242870882No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6210.11039666356750108No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA5870.1043524017940791No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG5710.10150804331246878No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG8650.049.5665932
GTACCTG10450.041.598651
ACCTGGG10850.038.4185523
GTGTAGC3900.038.172281
AATGCCG902.3924332E-433.0532348
ATGCCGG902.3924332E-433.0532349
GTAGCCC3800.032.9085243
CCTGGGG6950.032.559034
GTACACG750.00389315931.7593351
TAGGACC8850.030.279034
GACGCAA400.00536399729.74791176-77
GAGTACT30350.028.03262712-13
TGTAGCC5100.028.0229422
TACATGG42550.027.8500652
GTACATG42800.027.8265231
AGACCCG1107.7093026E-427.0676145
CTACACT3100.026.8929864
AGTACTT31050.026.82581312-13
TAGCCCT5900.026.2418254
ACATGGG42300.025.621453
Produced by FastQC (version 0.11.5)