FastQCFastQC Report
Fri 27 May 2016
SRR939959_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939959_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1717885
Sequences flagged as poor quality0
Sequence length125
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC8600.01289.0365108-109
CCTCGCC9000.01007.7921108-109
CCGCGCC7700.0916.1746108-109
TTGTTCG3150.0719.85156108-109
GGTCGCC2550.0691.622108-109
CTCCGCC4900.0683.8696110-111
CGCCGCT2950.0619.59216110-111
CGTCGCC3450.0584.2273108-109
CGCCGTC1051.1083801E-4580.253110-111
CATCGCG1355.546008E-7559.8845108-109
CGTCGCT1706.509781E-7537.58734110-111
CGTCGGT1251.8691254E-4487.4125110-111
CCGTTCG1051.9602847E-4479.90103108-109
CGCCTCT3202.910383E-11475.98877110-111
CGTTGCA1352.3539836E-4451.3079110-111
TTATTCG1152.5746386E-4438.1705108-109
CGTGGTT2859.511496E-9427.5548110-111
CGCCGCC5850.0416.5919110-111
CGTCGCG754.826925E-7406.1771110-111
CCGCACC6350.0396.76855108-109
Produced by FastQC (version 0.11.5)