FastQCFastQC Report
Fri 27 May 2016
SRR939959_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939959_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1717885
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38940.2266740788818809No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24650.14349039662142693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17570.10227692773381221No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA78600.037.4480631
ATCAACG91450.031.9840983
TCAACGC92200.031.5947044
TATCAAC92900.031.549012
CAACGCA92800.031.3904275
GGTATCA53250.031.3302151
AACGCAG94650.030.8003226
GTACATG72250.029.0288431
TACATGG74200.028.1799072
ACGCAGA106150.027.3514027
CGCAGAG106850.027.0608568
ACATGGG75650.026.7735713
GAGTACT52600.025.1665712-13
AGTACTT56500.024.27181812-13
GCAGAGT120050.023.9862849
GTACCGT1250.001634669523.7979626
AGAGTAC93750.023.63930910-11
TACCTGG17600.023.3546162
GTACTTT56250.023.21758714-15
CATGGGG41700.022.999914
Produced by FastQC (version 0.11.5)